pcr 2.1-topo vector Search Results


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  • 99
    Thermo Fisher topo ta pcr 2 1 topo vector cloning kit
    Topo Ta Pcr 2 1 Topo Vector Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    topo ta pcr 2 1 topo vector cloning kit - by Bioz Stars, 2020-08
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    94
    Thermo Fisher pcr 2 1 topo vector
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Pcr 2 1 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 7104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector/product/Thermo Fisher
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    92
    Stratagene pcr 2 1 topo vector
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Pcr 2 1 Topo Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector/product/Stratagene
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    pcr 2 1 topo vector - by Bioz Stars, 2020-08
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    85
    Thermo Fisher pcr 2 1 topo vector kits
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Pcr 2 1 Topo Vector Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector kits/product/Thermo Fisher
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    pcr 2 1 topo vector kits - by Bioz Stars, 2020-08
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    85
    Thermo Fisher topoisomerase activated pcr 2 1 topo vector
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Topoisomerase Activated Pcr 2 1 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific pcr 2 1 topo vector
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Pcr 2 1 Topo Vector, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector/product/Fisher Scientific
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo vector - by Bioz Stars, 2020-08
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    92
    Meridian Life Science pcr 2 1 topo vector
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Pcr 2 1 Topo Vector, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector/product/Meridian Life Science
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    Price from $9.99 to $1999.99
    pcr 2 1 topo vector - by Bioz Stars, 2020-08
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    90
    Thermo Fisher pcr 2 1 topo vector cloning vector
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Pcr 2 1 Topo Vector Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector cloning vector/product/Thermo Fisher
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    Price from $9.99 to $1999.99
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    84
    Thermo Fisher topo ta cloning pcr 2 1 topo vector kit
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Topo Ta Cloning Pcr 2 1 Topo Vector Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/topo ta cloning pcr 2 1 topo vector kit/product/Thermo Fisher
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    86
    Thermo Fisher wild type genomic sequenceinto pcr 2 1 topo vector
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Wild Type Genomic Sequenceinto Pcr 2 1 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR 2.1-TOPO vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.

    Journal: Molecular Pathology

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype

    doi:

    Figure Lengend Snippet: (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR 2.1-TOPO vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.

    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen).

    Techniques: Clone Assay, Polymerase Chain Reaction, Plasmid Preparation

    Genomic organization and transcription of T. cruzi CaNA2 gene. A) Southern blot hybridization. CL strain genomic DNA was digested with the indicated restriction enzymes, electrophoresed in agarose gel (1), transferred to Hybond-N membrane and hybridized to the 32 P-labeled TcCaNA2 gene probe (2). Size markers are indicated in kilobases (kb). B) Chromoblot hybridization. Chromosomal bands of epimastigotes from clone CL-Brener (CLB) and CL strain (CL) were separated by PFGE, stained with ethidium bromide (1), transferred to Hybond-N membrane and hybridized with the same probe described above (2). Size markers are indicated in megabase pair (Mbp). C) Northern blot hybridization. Total RNA (12 µg) extracted from different developmental forms of T. cruzi CL strain (TCT: tissue culture trypomastigotes, MT: metacyclic trypomastigotes, A: amastigotes, E: epimastigotes) was subjected to formaldehyde-agarose gel electrophoresis, stained with ethidium bromide (1), transferred to Hybond-N membrane and hybridized with the same probe described above (2). M, molecular size markers, are indicated in kilobases (kb). D) Amplification of TcCaNA2 gene by RT-PCR (upper) and immunoblotting of TcCaNA2 protein expression (lower) in different developmental forms of T. cruzi . M, molecular size markers (kb). (+): positive control, corresponds to plasmid pCR 2.1-TOPO with TcCaNA2.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: A Cytoplasmic New Catalytic Subunit of Calcineurin in Trypanosoma cruzi and Its Molecular and Functional Characterization

    doi: 10.1371/journal.pntd.0002676

    Figure Lengend Snippet: Genomic organization and transcription of T. cruzi CaNA2 gene. A) Southern blot hybridization. CL strain genomic DNA was digested with the indicated restriction enzymes, electrophoresed in agarose gel (1), transferred to Hybond-N membrane and hybridized to the 32 P-labeled TcCaNA2 gene probe (2). Size markers are indicated in kilobases (kb). B) Chromoblot hybridization. Chromosomal bands of epimastigotes from clone CL-Brener (CLB) and CL strain (CL) were separated by PFGE, stained with ethidium bromide (1), transferred to Hybond-N membrane and hybridized with the same probe described above (2). Size markers are indicated in megabase pair (Mbp). C) Northern blot hybridization. Total RNA (12 µg) extracted from different developmental forms of T. cruzi CL strain (TCT: tissue culture trypomastigotes, MT: metacyclic trypomastigotes, A: amastigotes, E: epimastigotes) was subjected to formaldehyde-agarose gel electrophoresis, stained with ethidium bromide (1), transferred to Hybond-N membrane and hybridized with the same probe described above (2). M, molecular size markers, are indicated in kilobases (kb). D) Amplification of TcCaNA2 gene by RT-PCR (upper) and immunoblotting of TcCaNA2 protein expression (lower) in different developmental forms of T. cruzi . M, molecular size markers (kb). (+): positive control, corresponds to plasmid pCR 2.1-TOPO with TcCaNA2.

    Article Snippet: The amplification product was cloned in the vector pCR 2.1-TOPO (TOPO Cloning Vector Kit for sequencing, Invitrogen by Life Technologies) according to the manufacturer's instructions.

    Techniques: Southern Blot, Hybridization, Agarose Gel Electrophoresis, Labeling, Staining, Northern Blot, Amplification, Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control, Plasmid Preparation, Polymerase Chain Reaction