Journal:

Article Title: Novel ?-Lactamase Genes from Two Environmental Isolates of Vibrio harveyi

doi:

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Ampicillin-resistant (Amp r ) V. harveyi strains were grown routinely in LB media containing 100 μg of ampicillin/ml. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant characteristic(s) Source Geographic location Reference or source Strains E. coli TOP10 F − mcrA ( mrr-hsdRMS - mcrBC ) φ80 lac2 ΔM15 Δ lac74 recA1 deoR araD139 Δ( ara-leu ) 7697 galU galK rpsL endA1 nupG ) Invitrogen V. harveyi isolates W3B Amp r Seawater near shrimp hatchery Besuki, northern coast of East Java E 2 Amp r Shrimp egg Besuki, East Java GCB Amp r Shrimp gut Besuki, East Java P1B Amp r Shrimp larvae Besuki, East Java M 1 Amp r Mysis (prawn larval stage) Besuki, East Java 30 M3.4L Amp r Mysis (prawn larval stage) Labuhan, northern coast of West Java AP5 Amp r Seawater Pacitan, southern coast of East Java AP6 Amp r Seawater Pacitan, East Java HB3 Amp r Seawater Pacitan, East Java Plasmids pCR 2.1-TOPO PCR TOPO vector Invitrogen pAS900 Km r Amp s ; derivative of pCR 2.1-TOPO (Invitrogen) cloning vector carrying an Xmn I- Sca I deletion in ampicillin resistance gene A. Suwanto (unpublished) pVHA1 pAS900 recombinant plasmid containing a 1.1-kb Hin dIII chromosomal fragment from W3B This study pVHA4 pAS900 recombinant plasmid containing a 1.1-kb Hin dIII chromosomal fragment from HB3 This study Open in a separate window Bacterial strains and plasmids

Techniques: Plasmid Preparation, Northern Blot, Clone Assay, Recombinant

Journal:

Article Title: Improvement and Optimization of Two Engineered Phage Resistance Mechanisms in Lactococcus lactis

doi: 10.1128/AEM.67.2.608-616.2001

Figure Lengend Snippet: Bacteriophages, bacterial strains, and plasmids used in this study

Article Snippet: The bacterial strains, bacteriophages, and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Phage, bacterial strain, or plasmid Relevant feature(s) Source or reference Phages Tuc2009 P335 type lysogenic phage of L. lactis subsp. cremoris UC509 4 Q30 P335 type lytic phage of L. lactis subsp. lactis 33 Q33 P335 type lytic phage of L. lactis subsp. lactis 33 ul36 P335 type lytic phage of L. lactis subsp. cremoris 34 E. coli strains JM101 Transformation host New England Biolabs TOP10 Transformation host Invitrogen L. lactis strains UC509.9 Prophage-cured derivative of L. lactis subsp. cremoris UC509, host for Tuc2009 11 SMQ86 Propagating host for phages Q30, Q33, and u136 15 Plasmids pCR 2.1-TOPO Cloning vector, Ap r Invitrogen pNZ8048 E. coli-L. lactis high-copy-number shuttle vector, Cm r (Per − ) 13 pNZRep-Cii pNZ8048 containing one copy of ori 2009 (Per + ) 31 pNZRep-3 pNZ8048 containing rep 2009 in antisense orientation (Per + ) 31 pSMG-1 pNZ8048 containing two copies of ori 2009 (Per + ) This study pSMG-2 pNZ8048 containing three copies of ori 2009 (Per + ) This study pNZ44 pNZ8048 containing constitutive P44 promoter from L. lactis chromosome This study pNZ44- rep 2009 pNZ44 containing rep 2009 in sense orientation (Per + ) This study pNZ44- orf14 rev pNZ44 containing orf14 in antisense orientation This study pNZ44- orf15 rev pNZ44 containing orf15 in antisense orientation This study pNZ44- rep 2009 rev pNZ44 containing rep 2009 in antisense orientation This study pNZ44- orf17 rev pNZ44 containing orf17 in antisense orientation This study pNZ44- orf18 rev pNZ44 containing orf18 in antisense orientation This study pNZ44- orf19 rev pNZ44 containing orf19 in antisense orientation This study pNZ44- msp1 rev pNZ44 containing msp1 in antisense orientation This study pNZ44- msp2 rev pNZ44 containing msp2 in antisense orientation This study Open in a separate window Bacteriophages, bacterial strains, and plasmids used in this study

Techniques: Plasmid Preparation, Transformation Assay, Clone Assay

Journal:

Article Title: Improvement and Optimization of Two Engineered Phage Resistance Mechanisms in Lactococcus lactis

doi: 10.1128/AEM.67.2.608-616.2001

Figure Lengend Snippet: Southern blot analysis showing hybridization of labeled Tuc2009 DNA probes to DNA fragments of bacteriophages Q30, Q33, and u136. (a) Ethidium bromide-stained agarose gel with EcoRI-restricted phage DNA. (b through g) Southern blot analysis. The Tuc2009 DNA probe used is indicated above each panel. Lane M contained DNA molecular weight marker X (Boehringer). Lanes A, Tuc2009; lanes B, Q30; lanes C, Q33; lanes D, ul36.

Article Snippet: The bacterial strains, bacteriophages, and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Phage, bacterial strain, or plasmid Relevant feature(s) Source or reference Phages Tuc2009 P335 type lysogenic phage of L. lactis subsp. cremoris UC509 4 Q30 P335 type lytic phage of L. lactis subsp. lactis 33 Q33 P335 type lytic phage of L. lactis subsp. lactis 33 ul36 P335 type lytic phage of L. lactis subsp. cremoris 34 E. coli strains JM101 Transformation host New England Biolabs TOP10 Transformation host Invitrogen L. lactis strains UC509.9 Prophage-cured derivative of L. lactis subsp. cremoris UC509, host for Tuc2009 11 SMQ86 Propagating host for phages Q30, Q33, and u136 15 Plasmids pCR 2.1-TOPO Cloning vector, Ap r Invitrogen pNZ8048 E. coli-L. lactis high-copy-number shuttle vector, Cm r (Per − ) 13 pNZRep-Cii pNZ8048 containing one copy of ori 2009 (Per + ) 31 pNZRep-3 pNZ8048 containing rep 2009 in antisense orientation (Per + ) 31 pSMG-1 pNZ8048 containing two copies of ori 2009 (Per + ) This study pSMG-2 pNZ8048 containing three copies of ori 2009 (Per + ) This study pNZ44 pNZ8048 containing constitutive P44 promoter from L. lactis chromosome This study pNZ44- rep 2009 pNZ44 containing rep 2009 in sense orientation (Per + ) This study pNZ44- orf14 rev pNZ44 containing orf14 in antisense orientation This study pNZ44- orf15 rev pNZ44 containing orf15 in antisense orientation This study pNZ44- rep 2009 rev pNZ44 containing rep 2009 in antisense orientation This study pNZ44- orf17 rev pNZ44 containing orf17 in antisense orientation This study pNZ44- orf18 rev pNZ44 containing orf18 in antisense orientation This study pNZ44- orf19 rev pNZ44 containing orf19 in antisense orientation This study pNZ44- msp1 rev pNZ44 containing msp1 in antisense orientation This study pNZ44- msp2 rev pNZ44 containing msp2 in antisense orientation This study Open in a separate window Bacteriophages, bacterial strains, and plasmids used in this study

Techniques: Southern Blot, Hybridization, Labeling, Staining, Agarose Gel Electrophoresis, Molecular Weight, Marker