Journal:

Article Title: Novel ?-Lactamase Genes from Two Environmental Isolates of Vibrio harveyi

doi:

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Ampicillin-resistant (Amp r ) V. harveyi strains were grown routinely in LB media containing 100 μg of ampicillin/ml. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant characteristic(s) Source Geographic location Reference or source Strains E. coli TOP10 F − mcrA ( mrr-hsdRMS - mcrBC ) φ80 lac2 ΔM15 Δ lac74 recA1 deoR araD139 Δ( ara-leu ) 7697 galU galK rpsL endA1 nupG ) Invitrogen V. harveyi isolates W3B Amp r Seawater near shrimp hatchery Besuki, northern coast of East Java E 2 Amp r Shrimp egg Besuki, East Java GCB Amp r Shrimp gut Besuki, East Java P1B Amp r Shrimp larvae Besuki, East Java M 1 Amp r Mysis (prawn larval stage) Besuki, East Java 30 M3.4L Amp r Mysis (prawn larval stage) Labuhan, northern coast of West Java AP5 Amp r Seawater Pacitan, southern coast of East Java AP6 Amp r Seawater Pacitan, East Java HB3 Amp r Seawater Pacitan, East Java Plasmids pCR 2.1-TOPO PCR TOPO vector Invitrogen pAS900 Km r Amp s ; derivative of pCR 2.1-TOPO (Invitrogen) cloning vector carrying an Xmn I- Sca I deletion in ampicillin resistance gene A. Suwanto (unpublished) pVHA1 pAS900 recombinant plasmid containing a 1.1-kb Hin dIII chromosomal fragment from W3B This study pVHA4 pAS900 recombinant plasmid containing a 1.1-kb Hin dIII chromosomal fragment from HB3 This study Open in a separate window Bacterial strains and plasmids

Techniques: Plasmid Preparation, Northern Blot, Clone Assay, Recombinant

Journal:

Article Title: Improvement and Optimization of Two Engineered Phage Resistance Mechanisms in Lactococcus lactis

doi: 10.1128/AEM.67.2.608-616.2001

Figure Lengend Snippet: Bacteriophages, bacterial strains, and plasmids used in this study

Article Snippet: The bacterial strains, bacteriophages, and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Phage, bacterial strain, or plasmid Relevant feature(s) Source or reference Phages Tuc2009 P335 type lysogenic phage of L. lactis subsp. cremoris UC509 4 Q30 P335 type lytic phage of L. lactis subsp. lactis 33 Q33 P335 type lytic phage of L. lactis subsp. lactis 33 ul36 P335 type lytic phage of L. lactis subsp. cremoris 34 E. coli strains JM101 Transformation host New England Biolabs TOP10 Transformation host Invitrogen L. lactis strains UC509.9 Prophage-cured derivative of L. lactis subsp. cremoris UC509, host for Tuc2009 11 SMQ86 Propagating host for phages Q30, Q33, and u136 15 Plasmids pCR 2.1-TOPO Cloning vector, Ap r Invitrogen pNZ8048 E. coli-L. lactis high-copy-number shuttle vector, Cm r (Per − ) 13 pNZRep-Cii pNZ8048 containing one copy of ori 2009 (Per + ) 31 pNZRep-3 pNZ8048 containing rep 2009 in antisense orientation (Per + ) 31 pSMG-1 pNZ8048 containing two copies of ori 2009 (Per + ) This study pSMG-2 pNZ8048 containing three copies of ori 2009 (Per + ) This study pNZ44 pNZ8048 containing constitutive P44 promoter from L. lactis chromosome This study pNZ44- rep 2009 pNZ44 containing rep 2009 in sense orientation (Per + ) This study pNZ44- orf14 rev pNZ44 containing orf14 in antisense orientation This study pNZ44- orf15 rev pNZ44 containing orf15 in antisense orientation This study pNZ44- rep 2009 rev pNZ44 containing rep 2009 in antisense orientation This study pNZ44- orf17 rev pNZ44 containing orf17 in antisense orientation This study pNZ44- orf18 rev pNZ44 containing orf18 in antisense orientation This study pNZ44- orf19 rev pNZ44 containing orf19 in antisense orientation This study pNZ44- msp1 rev pNZ44 containing msp1 in antisense orientation This study pNZ44- msp2 rev pNZ44 containing msp2 in antisense orientation This study Open in a separate window Bacteriophages, bacterial strains, and plasmids used in this study

Techniques: Plasmid Preparation, Transformation Assay, Clone Assay

Journal:

Article Title: Improvement and Optimization of Two Engineered Phage Resistance Mechanisms in Lactococcus lactis

doi: 10.1128/AEM.67.2.608-616.2001

Figure Lengend Snippet: Southern blot analysis showing hybridization of labeled Tuc2009 DNA probes to DNA fragments of bacteriophages Q30, Q33, and u136. (a) Ethidium bromide-stained agarose gel with EcoRI-restricted phage DNA. (b through g) Southern blot analysis. The Tuc2009 DNA probe used is indicated above each panel. Lane M contained DNA molecular weight marker X (Boehringer). Lanes A, Tuc2009; lanes B, Q30; lanes C, Q33; lanes D, ul36.

Article Snippet: The bacterial strains, bacteriophages, and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Phage, bacterial strain, or plasmid Relevant feature(s) Source or reference Phages Tuc2009 P335 type lysogenic phage of L. lactis subsp. cremoris UC509 4 Q30 P335 type lytic phage of L. lactis subsp. lactis 33 Q33 P335 type lytic phage of L. lactis subsp. lactis 33 ul36 P335 type lytic phage of L. lactis subsp. cremoris 34 E. coli strains JM101 Transformation host New England Biolabs TOP10 Transformation host Invitrogen L. lactis strains UC509.9 Prophage-cured derivative of L. lactis subsp. cremoris UC509, host for Tuc2009 11 SMQ86 Propagating host for phages Q30, Q33, and u136 15 Plasmids pCR 2.1-TOPO Cloning vector, Ap r Invitrogen pNZ8048 E. coli-L. lactis high-copy-number shuttle vector, Cm r (Per − ) 13 pNZRep-Cii pNZ8048 containing one copy of ori 2009 (Per + ) 31 pNZRep-3 pNZ8048 containing rep 2009 in antisense orientation (Per + ) 31 pSMG-1 pNZ8048 containing two copies of ori 2009 (Per + ) This study pSMG-2 pNZ8048 containing three copies of ori 2009 (Per + ) This study pNZ44 pNZ8048 containing constitutive P44 promoter from L. lactis chromosome This study pNZ44- rep 2009 pNZ44 containing rep 2009 in sense orientation (Per + ) This study pNZ44- orf14 rev pNZ44 containing orf14 in antisense orientation This study pNZ44- orf15 rev pNZ44 containing orf15 in antisense orientation This study pNZ44- rep 2009 rev pNZ44 containing rep 2009 in antisense orientation This study pNZ44- orf17 rev pNZ44 containing orf17 in antisense orientation This study pNZ44- orf18 rev pNZ44 containing orf18 in antisense orientation This study pNZ44- orf19 rev pNZ44 containing orf19 in antisense orientation This study pNZ44- msp1 rev pNZ44 containing msp1 in antisense orientation This study pNZ44- msp2 rev pNZ44 containing msp2 in antisense orientation This study Open in a separate window Bacteriophages, bacterial strains, and plasmids used in this study

Techniques: Southern Blot, Hybridization, Labeling, Staining, Agarose Gel Electrophoresis, Molecular Weight, Marker

Journal:

Article Title: DapE Can Function as an Aspartyl Peptidase in the Presence of Mn 2+

doi: 10.1128/JB.185.16.4748-4754.2003

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Liquid cultures were aerated by shaking on a rotary shaker, and all growth incubations were at 37°C. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Genotype or description Source or reference Strains S. enterica serovar Typhimurium TN1379 leuBCD485 Lab collection TN1715 leuBCD485 supQ302 Δ( proAB pepD ) pepN90 pepA16 pepB11 pepP1 pepQ1 pepT1 Lab collection TN5307 As TN1379 plus vector pSE380 Lab collection TN5538 leuBCD485 iaaA1 :: chl Lab collection TN5771 As TN1379 plus pKD46 This study TN5834 leuBCD485 pepT7 :: MudJ iadA50 :: chl This study TN5860 As TN1715 plus iadA50 :: chl This study TN5862 As TN5860 plus iadA100 This study TN5874 leuBCD485 pepE25 :: chl This study TN5875 As TN5862 plus pepE25 :: chl This study TN5879 As TN5875 plus pepE50 This study TN5889 As TN5879 plus iaaA1 :: chl This study TN5891 As TN5889 plus Δ sitA This study TN5893 leuBCD485 pepT7 :: MudJ plus pDK46 This study TN5896 As TN5891 plus dapE2 This study TN5909 As TN5891 plus dapE1 :: kan This study TN5910 As TN1379 plus dapE1 :: kan This study TN5911 As TN5889 plus dapE1 :: kan This study TN5934 As TN5911 plus pSE380 This study TN5935 As TN5911 plus pCM655 This study TN5936 As TN5918 plus pSE380 This study E. coli DH5α φ80 lacZ ΔM15 Δ( lacZYA ) U169 endA1 hsdR17 (r k − m k − ) supE44 thi-1 recA1 gyrA96 relA1 Gibco, BRL Top10F′ F − mcr A Δ( mrr-hsdRMS-mcrBC ) φ80 lacZ ΔM15 Δ lacX74 rec A1 deoR araD139 d(ara-leu)7697 galU galK rpsL (Str r ) endA1 nupG Invitrogen BL21 F − ompT hsdSb (r B − m B − ) gal dcm (DE3) Novagen TN5697 BL21/pCM652 This study Plasmids pCR 2.1-TOPO Cloning vector for PCR products Invitrogen pET28 T7 promoter Novagen pSE380 tac promoter Stratagene pKD46 λ Red recombinase B. L. Wanner pKD2 kan flanked by FRT recombinase sites B. L. Wanner pKD3 cat flanked by FRT recombinase sites B. L. Wanner pCM651 dapE-his 6 on pCR2.1 TOPO This study pCM652 dapE-his 6 on pET28 This study pCM653 dapE on pCR2.1 TOPO This study pCM655 dapE on pSE380 This study Open in a separate window Bacterial strains and plasmids used in this study

Techniques: Plasmid Preparation, Clone Assay