pcmv-lacz plasmid Search Results


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  • 85
    Thermo Fisher β galactosidase expression plasmid pcmv lacz
    β Galactosidase Expression Plasmid Pcmv Lacz, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β galactosidase expression plasmid pcmv lacz/product/Thermo Fisher
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    β galactosidase expression plasmid pcmv lacz - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    88
    PlasmidFactory copy plasmid pcmv lacz
    Growth curve for E. coli <t>DH5α-pCMV-lacZ</t> . The growth curve based on OD 600 measurements is marked by small black circles and the appropriate best-fit curve is indicated in blue. The harvesting time points T 1 , T 2 , and T 3 are presented as bold big points. The minimum–maximum area, within which the measurement values have to reside, is bordered by the red curve (minimal measured OD 600 value) and the green curve (maximal measured OD 600 value).
    Copy Plasmid Pcmv Lacz, supplied by PlasmidFactory, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/copy plasmid pcmv lacz/product/PlasmidFactory
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    copy plasmid pcmv lacz - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    85
    Stratagene plasmid pcmv lacz
    Growth curve for E. coli <t>DH5α-pCMV-lacZ</t> . The growth curve based on OD 600 measurements is marked by small black circles and the appropriate best-fit curve is indicated in blue. The harvesting time points T 1 , T 2 , and T 3 are presented as bold big points. The minimum–maximum area, within which the measurement values have to reside, is bordered by the red curve (minimal measured OD 600 value) and the green curve (maximal measured OD 600 value).
    Plasmid Pcmv Lacz, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pcmv lacz/product/Stratagene
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    plasmid pcmv lacz - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    84
    Addgene inc pcmv gfp frt lacz plasmid
    Growth curve for E. coli <t>DH5α-pCMV-lacZ</t> . The growth curve based on OD 600 measurements is marked by small black circles and the appropriate best-fit curve is indicated in blue. The harvesting time points T 1 , T 2 , and T 3 are presented as bold big points. The minimum–maximum area, within which the measurement values have to reside, is bordered by the red curve (minimal measured OD 600 value) and the green curve (maximal measured OD 600 value).
    Pcmv Gfp Frt Lacz Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv gfp frt lacz plasmid/product/Addgene inc
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcmv gfp frt lacz plasmid - by Bioz Stars, 2020-09
    84/100 stars
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    88
    Promega pcmv lacz plasmid
    Growth curve for E. coli <t>DH5α-pCMV-lacZ</t> . The growth curve based on OD 600 measurements is marked by small black circles and the appropriate best-fit curve is indicated in blue. The harvesting time points T 1 , T 2 , and T 3 are presented as bold big points. The minimum–maximum area, within which the measurement values have to reside, is bordered by the red curve (minimal measured OD 600 value) and the green curve (maximal measured OD 600 value).
    Pcmv Lacz Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv lacz plasmid/product/Promega
    Average 88 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    pcmv lacz plasmid - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Growth curve for E. coli DH5α-pCMV-lacZ . The growth curve based on OD 600 measurements is marked by small black circles and the appropriate best-fit curve is indicated in blue. The harvesting time points T 1 , T 2 , and T 3 are presented as bold big points. The minimum–maximum area, within which the measurement values have to reside, is bordered by the red curve (minimal measured OD 600 value) and the green curve (maximal measured OD 600 value).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: ColE1-Plasmid Production in Escherichia coli: Mathematical Simulation and Experimental Validation

    doi: 10.3389/fbioe.2015.00127

    Figure Lengend Snippet: Growth curve for E. coli DH5α-pCMV-lacZ . The growth curve based on OD 600 measurements is marked by small black circles and the appropriate best-fit curve is indicated in blue. The harvesting time points T 1 , T 2 , and T 3 are presented as bold big points. The minimum–maximum area, within which the measurement values have to reside, is bordered by the red curve (minimal measured OD 600 value) and the green curve (maximal measured OD 600 value).

    Article Snippet: Bacterial strain and plasmids The Escherichia coli strain DH5α (F–Φ80lac ZΔM15 Δ(lac ZYA-arg F) U169 rec A1 end A1 hsd R17 (rK−, mK+) pho A sup E44 λ− thi -1 gyr A96 rel A1) (Source: Plasmid Factory, Bielefeld, Germany) was used as a host strain for transformation of the high copy plasmid pCMV-lacZ (Source: Plasmid Factory, Bielefeld, Germany) as well as the low copy plasmid pSUP 201-3 (Simon et al., ). pCMV-lacZ is a ColE1-derived high copy plasmid for therapeutic pDNA production with a size of 7164 bp.

    Techniques:

    Antitumor effects of nontherapeutic plasmid. ( a ) Tumor growth curve of CT26 tumors treated with pCMV backbone, pCMV lacZ, enhanced expression vector (pEEV) backbone, pEEV lacZ, and an untreated tumor growing on Balb/c mice ( n = 6 per group) is presented as tumor diameter measured. Tumor volume was calculated as previously described. Data presented are means ± SEM of six individual tumors. *Compared to the untreated group and pEEV lacZ, * P

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment

    doi: 10.1038/mtm.2014.12

    Figure Lengend Snippet: Antitumor effects of nontherapeutic plasmid. ( a ) Tumor growth curve of CT26 tumors treated with pCMV backbone, pCMV lacZ, enhanced expression vector (pEEV) backbone, pEEV lacZ, and an untreated tumor growing on Balb/c mice ( n = 6 per group) is presented as tumor diameter measured. Tumor volume was calculated as previously described. Data presented are means ± SEM of six individual tumors. *Compared to the untreated group and pEEV lacZ, * P

    Article Snippet: Plasmids For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter.

    Techniques: Plasmid Preparation, Expressing, Mouse Assay

    β-galactosidase expression in murine tissue. ( a ) Copy numbers of lacZ transgene ascertained by quantitative polymerase chain reaction (PCR) in murine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNA 2 days after electroporation obtained from MF1-nu/nu mouse tissue. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each quantiative PCR (qPCR). qPCR values are means ± standard error of the mean (SEM) of triplicate measurements. A comparison of enhanced expression vector (pEEV) (light gray bars) and pCMV (dark gray bars) samples were performed. *** P

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment

    doi: 10.1038/mtm.2014.12

    Figure Lengend Snippet: β-galactosidase expression in murine tissue. ( a ) Copy numbers of lacZ transgene ascertained by quantitative polymerase chain reaction (PCR) in murine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNA 2 days after electroporation obtained from MF1-nu/nu mouse tissue. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each quantiative PCR (qPCR). qPCR values are means ± standard error of the mean (SEM) of triplicate measurements. A comparison of enhanced expression vector (pEEV) (light gray bars) and pCMV (dark gray bars) samples were performed. *** P

    Article Snippet: Plasmids For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Electroporation, Plasmid Preparation

    β-Galactosidase expression in porcine tissue. ( a ) Representative image of β-galactosidase staining in porcine tissues. β-Galactosidase expression significantly increased in enhanced expression vector (pEEV) lacZ in all tissues been examined. (b) Evaluation of lacZ mRNA transcript expression by qRT-PCR in porcine tissue. Bar graph presenting the relative expression of the lacZ mRNA 2 days after electroporation. All qPCR data were normalized using 18S RNA as reference gene. Relative expression levels are plotted as means ± standard error of the mean (SEM) of triplicate measurements. pEEV (light gray bars) and pCMV (dark gray bars). pEEV lacZ expressed was significantly higher than pCMV lacZ. ( c ) Copy numbers of lacZ transgene ascertained by quantitative PCR in porcine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNAs 2 days after electroporation. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each qPCR. qPCR values are means ± SEM of triplicate measurements. A comparison of pEEV (light gray bars) and pCMV (dark gray bars) samples were performed.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment

    doi: 10.1038/mtm.2014.12

    Figure Lengend Snippet: β-Galactosidase expression in porcine tissue. ( a ) Representative image of β-galactosidase staining in porcine tissues. β-Galactosidase expression significantly increased in enhanced expression vector (pEEV) lacZ in all tissues been examined. (b) Evaluation of lacZ mRNA transcript expression by qRT-PCR in porcine tissue. Bar graph presenting the relative expression of the lacZ mRNA 2 days after electroporation. All qPCR data were normalized using 18S RNA as reference gene. Relative expression levels are plotted as means ± standard error of the mean (SEM) of triplicate measurements. pEEV (light gray bars) and pCMV (dark gray bars). pEEV lacZ expressed was significantly higher than pCMV lacZ. ( c ) Copy numbers of lacZ transgene ascertained by quantitative PCR in porcine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNAs 2 days after electroporation. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each qPCR. qPCR values are means ± SEM of triplicate measurements. A comparison of pEEV (light gray bars) and pCMV (dark gray bars) samples were performed.

    Article Snippet: Plasmids For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter.

    Techniques: Expressing, Staining, Plasmid Preparation, Quantitative RT-PCR, Electroporation, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction