pcmv b vector Search Results


90
Becton Dickinson vector pcmvb
Vector Pcmvb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+b+vector/vector+pcmvb/pm15856458-53-30-32
Average 90 stars, based on 1 article reviews
vector pcmvb - by Bioz Stars, 2026-09
90/100 stars
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90
Promega pcmv/β-gal reporter vector
( A ) Schematic representation of C/EBP binding sites within the 5′ flanking region of the human galectin-7 gene. A s eries of 5′ deletion constructs of the 1500 bp galectin-7 promoter region was generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were transfected in MCF-7 and MDA-MB-468 cells. Locations of the putative C/EBP binding sites in the promoter, as determined using the TFsearch computational tool, are shown as empty boxes. ( B ) Sequence analysis of the C/EBP binding sites located at positions -105-98 and the -145-140. ( C ) Mutated constructs of the C/EBPβ binding sites on the 200 bp galectin-7 promoter region were generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were co-transfected in MCF-7 cells and were compared to the wild-type p200-galectin-7 promoter. Transfection efficiency was normalized by co-transfection with <t>a</t> <t>β-galactosidase</t> reporter vector.
Pcmv/β Gal Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+b+vector/pcmv+b+gal+reporter+vector/pmc04008383-48-6-9
Average 90 stars, based on 1 article reviews
pcmv/β-gal reporter vector - by Bioz Stars, 2026-09
90/100 stars
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90
Becton Dickinson notiþblunt-ended xhoi-pcmvb vector
( A ) Schematic representation of C/EBP binding sites within the 5′ flanking region of the human galectin-7 gene. A s eries of 5′ deletion constructs of the 1500 bp galectin-7 promoter region was generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were transfected in MCF-7 and MDA-MB-468 cells. Locations of the putative C/EBP binding sites in the promoter, as determined using the TFsearch computational tool, are shown as empty boxes. ( B ) Sequence analysis of the C/EBP binding sites located at positions -105-98 and the -145-140. ( C ) Mutated constructs of the C/EBPβ binding sites on the 200 bp galectin-7 promoter region were generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were co-transfected in MCF-7 cells and were compared to the wild-type p200-galectin-7 promoter. Transfection efficiency was normalized by co-transfection with <t>a</t> <t>β-galactosidase</t> reporter vector.
Notiþblunt Ended Xhoi Pcmvb Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+b+vector/noti%C3%BEblunt+ended+xhoi+pcmvb+vector/pm19165234-33-21-24
Average 90 stars, based on 1 article reviews
notiþblunt-ended xhoi-pcmvb vector - by Bioz Stars, 2026-09
90/100 stars
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90
Becton Dickinson pcmvb vector dna
( A ) Schematic representation of C/EBP binding sites within the 5′ flanking region of the human galectin-7 gene. A s eries of 5′ deletion constructs of the 1500 bp galectin-7 promoter region was generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were transfected in MCF-7 and MDA-MB-468 cells. Locations of the putative C/EBP binding sites in the promoter, as determined using the TFsearch computational tool, are shown as empty boxes. ( B ) Sequence analysis of the C/EBP binding sites located at positions -105-98 and the -145-140. ( C ) Mutated constructs of the C/EBPβ binding sites on the 200 bp galectin-7 promoter region were generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were co-transfected in MCF-7 cells and were compared to the wild-type p200-galectin-7 promoter. Transfection efficiency was normalized by co-transfection with <t>a</t> <t>β-galactosidase</t> reporter vector.
Pcmvb Vector Dna, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+b+vector/pcmvb+vector+dna/pm16690914-63-6-9
Average 90 stars, based on 1 article reviews
pcmvb vector dna - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Schematic representation of C/EBP binding sites within the 5′ flanking region of the human galectin-7 gene. A s eries of 5′ deletion constructs of the 1500 bp galectin-7 promoter region was generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were transfected in MCF-7 and MDA-MB-468 cells. Locations of the putative C/EBP binding sites in the promoter, as determined using the TFsearch computational tool, are shown as empty boxes. ( B ) Sequence analysis of the C/EBP binding sites located at positions -105-98 and the -145-140. ( C ) Mutated constructs of the C/EBPβ binding sites on the 200 bp galectin-7 promoter region were generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were co-transfected in MCF-7 cells and were compared to the wild-type p200-galectin-7 promoter. Transfection efficiency was normalized by co-transfection with a β-galactosidase reporter vector.

Journal: PLoS ONE

Article Title: The CCAAT/Enhancer-Binding Protein Beta-2 Isoform (CEBPβ-2) Upregulates Galectin-7 Expression in Human Breast Cancer Cells

doi: 10.1371/journal.pone.0095087

Figure Lengend Snippet: ( A ) Schematic representation of C/EBP binding sites within the 5′ flanking region of the human galectin-7 gene. A s eries of 5′ deletion constructs of the 1500 bp galectin-7 promoter region was generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were transfected in MCF-7 and MDA-MB-468 cells. Locations of the putative C/EBP binding sites in the promoter, as determined using the TFsearch computational tool, are shown as empty boxes. ( B ) Sequence analysis of the C/EBP binding sites located at positions -105-98 and the -145-140. ( C ) Mutated constructs of the C/EBPβ binding sites on the 200 bp galectin-7 promoter region were generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were co-transfected in MCF-7 cells and were compared to the wild-type p200-galectin-7 promoter. Transfection efficiency was normalized by co-transfection with a β-galactosidase reporter vector.

Article Snippet: Transfection efficiency was measured using the pCMV/β-gal reporter vector (Promega, Madison, WI).

Techniques: Binding Assay, Construct, Generated, Clone Assay, Luciferase, Plasmid Preparation, Transfection, Sequencing, Cotransfection