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Image Search Results
Journal: PLoS ONE
Article Title: The CCAAT/Enhancer-Binding Protein Beta-2 Isoform (CEBPβ-2) Upregulates Galectin-7 Expression in Human Breast Cancer Cells
doi: 10.1371/journal.pone.0095087
Figure Lengend Snippet: ( A ) Schematic representation of C/EBP binding sites within the 5′ flanking region of the human galectin-7 gene. A s eries of 5′ deletion constructs of the 1500 bp galectin-7 promoter region was generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were transfected in MCF-7 and MDA-MB-468 cells. Locations of the putative C/EBP binding sites in the promoter, as determined using the TFsearch computational tool, are shown as empty boxes. ( B ) Sequence analysis of the C/EBP binding sites located at positions -105-98 and the -145-140. ( C ) Mutated constructs of the C/EBPβ binding sites on the 200 bp galectin-7 promoter region were generated and cloned into the pGL3 Basic luciferase reporter vector. The resulting plasmids were co-transfected in MCF-7 cells and were compared to the wild-type p200-galectin-7 promoter. Transfection efficiency was normalized by co-transfection with a β-galactosidase reporter vector.
Article Snippet: Transfection efficiency was measured using the
Techniques: Binding Assay, Construct, Generated, Clone Assay, Luciferase, Plasmid Preparation, Transfection, Sequencing, Cotransfection