pcm Search Results


jan 24  (ATCC)
92
ATCC jan 24
Jan 24, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/us09006538-894-7-5?v=ATCC
Average 92 stars, based on 1 article reviews
jan 24 - by Bioz Stars, 2026-08
92/100 stars
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pcm 1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc pcm 1
Pcm 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/pmc06980882-90-27-28?v=Cell+Signaling+Technology+Inc
Average 93 stars, based on 1 article reviews
pcm 1 - by Bioz Stars, 2026-08
93/100 stars
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pcm1  (Proteintech)
93
Proteintech pcm1
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Pcm1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/pm32402286-412-8-9?v=Proteintech
Average 93 stars, based on 1 article reviews
pcm1 - by Bioz Stars, 2026-08
93/100 stars
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rabbit anti pcm 1  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc rabbit anti pcm 1
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Rabbit Anti Pcm 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/pmc04289677-61-50-52?v=Cell+Signaling+Technology+Inc
Average 92 stars, based on 1 article reviews
rabbit anti pcm 1 - by Bioz Stars, 2026-08
92/100 stars
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acetaminophen mercapturate  (Toronto Research Chemicals)
93
Toronto Research Chemicals acetaminophen mercapturate
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Acetaminophen Mercapturate, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/pmc08535641-140-0-4?v=Toronto+Research+Chemicals
Average 93 stars, based on 1 article reviews
acetaminophen mercapturate - by Bioz Stars, 2026-08
93/100 stars
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pcmv myc vector  (OriGene)
90
OriGene pcmv myc vector
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Pcmv Myc Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/pmc05201008-385-12-15?v=OriGene
Average 90 stars, based on 1 article reviews
pcmv myc vector - by Bioz Stars, 2026-08
90/100 stars
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pcm  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology pcm
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Pcm, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/pm28821957-115-13-19?v=Santa+Cruz+Biotechnology
Average 90 stars, based on 1 article reviews
pcm - by Bioz Stars, 2026-08
90/100 stars
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pta  (ATCC)
92
ATCC pta
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Pta, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/us09936659-1098-32-29?v=ATCC
Average 92 stars, based on 1 article reviews
pta - by Bioz Stars, 2026-08
92/100 stars
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s mitis gifu 12458 t  (ATCC)
90
ATCC s mitis gifu 12458 t
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
S Mitis Gifu 12458 T, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/pmc02918946-244-121-137?v=ATCC
Average 90 stars, based on 1 article reviews
s mitis gifu 12458 t - by Bioz Stars, 2026-08
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core solution  (Chem Impex International)
95
Chem Impex International core solution
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Core Solution, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/bio_rxiv__2025__04__03__647129-76-1-9?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
core solution - by Bioz Stars, 2026-08
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petduet 1mcs1 dmkin432  (Addgene inc)
90
Addgene inc petduet 1mcs1 dmkin432
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Petduet 1mcs1 Dmkin432, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/10__1103_slash_physrevx__7__041037-147-32-30?v=Addgene+inc
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pcm 2000 cslm  (Nikon)
95
Nikon pcm 2000 cslm
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Pcm 2000 Cslm, supplied by Nikon, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcm/pmc07111329-198-17-16?v=Nikon
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pcm 2000 cslm - by Bioz Stars, 2026-08
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Image Search Results


Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, PCM1, and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.

Journal: Cell reports

Article Title: CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

doi: 10.1016/j.celrep.2020.107630

Figure Lengend Snippet: Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, PCM1, and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.

Article Snippet: Primary antibodies used for immunoblotting were rabbit anti PCM1 (Proteintech, 19856-1-AP) at 1:500, rabbit anti-beta-actin (Cell Signaling Technology) at 1:10000, mouse anti alpha-tubulin (Sigma, DM1A) at 1:5000, anti-CCDC57 (Sigma HPA023344) at 1:1000, anti-c-Myc (clone 9E10) at 1:500, rabbit anticapsase-3 (Proteintech) at 1:500 and mouse anti-acetylated tubulin (clone 6-11B, 32270, Thermo Fischer) at 1:5000. anti-PCM1 and anti-GFP antibodies were generated and used for immunobloting as previously described (Firat-Karalar et al., 2014).

Techniques: Control, Staining, Expressing, Stable Transfection, Transfection