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Image Search Results
Journal: Molecular Plant Pathology
Article Title: 6‐Hydroxynicotinic Acid From Cucumis melo Inhibits Prehaustorium Formation in Phelipanche aegyptiaca via Disruption of Auxin Signalling Pathway
doi: 10.1111/mpp.70137
Figure Lengend Snippet: Auxin inhibitors halt prehaustorium development in Phelipanche aegyptiaca . (A) The radicle tips in P. aegyptiaca after 3 days of treatment with gradient concentrations of p ‐chlorophenoxyisobutyric acid (PCIB) and 2,3,5‐triiodobenzoic acid (TIBA) (0.2, 0.1, 0.01 mM) combined with 2,6‐dimethoxy‐1,4‐benzoquinone (DMBQ) (0.01 mM) or indole‐3‐acetic acid (IAA) (0.1 mM). The corresponding control treatments included 0.01 mM DMBQ or 0.1 mM IAA alone. Red arrows indicate swollen radicle tips under various treatments. Scale bar: 500 μm. (B) Expansion rates of radicle tips. (C) Radicle length ( n = 21 seeds). (D) Endogenous IAA content. Data are presented as the mean ± SD (three biological replicates). Groups marked by different letters differ significantly ( p < 0.05) using one‐way ANOVA and Duncan's test. (E) Heatmap showing the hierarchical clustering of auxin‐related gene expression across six treatments: DMBQ + PCIB, DMBQ + TIBA, DMBQ, IAA + PCIB, IAA + TIBA and IAA. The raw data were normalised using Z‐score standardisation. The value ‘0’ represents the overall average expression level of each hormone across all samples. (F) Venn diagram showing the overlap of differentially expressed genes (DEGs) identified from pairwise comparisons: DMBQ + PCIB vs. DMBQ, DMBQ + TIBA vs. DMBQ, IAA + PCIB vs. IAA and IAA + TIBA vs. IAA. (G) KEGG enrichment analysis of 1106 DEGs common to the comparisons.
Article Snippet: The experimental groups received 0.1 mM IAA and 0.01 mM DMBQ mixed with 0.2, 0.1, or 0.01 mM
Techniques: Control, Gene Expression, Expressing