Journal: Molecular Medicine Reports

Article Title: MicroRNA-124 suppresses cell proliferation and invasion of triple negative breast cancer cells by targeting STAT3

doi: 10.3892/mmr.2019.10044

Figure Lengend Snippet: STAT3 may be a downstream of miR-124 in breast cancer. (A) The potential miR-124 binding area in the WT 3′UTR of STAT3 mRNA, the corresponding MUT sequence is in red. (B) Reverse transcription quantitative polymerase chain reaction was performed to measure the expression of miR-124 in human breast tissues. (C) The correlation between expression of miR-124 and STAT3 in 20 human breast tissue samples. **P<0.01 vs. control. STAT3, signal transducer and activator of transcription 3; UTR, untranslated region; miR-124, microRNA-124; WT, wide type; MUT, mutated.

Article Snippet: miRNA-124 mimics (miR-124), miRNA-124 inhibitor (inh-124), STAT3 overexpression plasmid [pcDNA3.0-STAT3 (plasmid-STAT3)] and corresponding negative controls [miR-NC, inh-NC and pcDNA3.0-empty vector (plasmid-NC)] were designed and purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China).

Techniques: Binding Assay, Sequencing, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Control

Journal: Molecular Medicine Reports

Article Title: MicroRNA-124 suppresses cell proliferation and invasion of triple negative breast cancer cells by targeting STAT3

doi: 10.3892/mmr.2019.10044

Figure Lengend Snippet: miR-124 negatively regulates the expression of STAT3 by direct interaction in breast cancer. (A) The expression of STAT3 mRNA was decreased by overexpression of miR-124 in MDA-MB-468 and MDA-MB-231 cells, while inhibition of miR-124 led to the upregulation of STAT3. (B) The corresponding western blot data of STAT3. Data values represent the relative intensity values. (C) A dual luciferase assay was performed following co-transfection with WT or MUT reporters and miR-124 or miR-NC in MDA-MB-468 cells. miR-124 overexpression lead to a significant decrease in the activity of the WT reporter but not the MUT reporter. Firefly luciferase activity was used to normalize the data. All data are presented as mean ± standard deviation of at least three independent experiments. *P<0.05 and **P<0.01 vs. control. miR-124, microRNA-124; STAT3, signal transducer and activator of transcription 3; inh, inhibitor; miR-124, microRNA-124; WT, wild type; MUT, mutant; NC, negative control.

Article Snippet: miRNA-124 mimics (miR-124), miRNA-124 inhibitor (inh-124), STAT3 overexpression plasmid [pcDNA3.0-STAT3 (plasmid-STAT3)] and corresponding negative controls [miR-NC, inh-NC and pcDNA3.0-empty vector (plasmid-NC)] were designed and purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China).

Techniques: Expressing, Over Expression, Inhibition, Western Blot, Luciferase, Cotransfection, Activity Assay, Standard Deviation, Control, Mutagenesis, Negative Control

Journal: Molecular Medicine Reports

Article Title: MicroRNA-124 suppresses cell proliferation and invasion of triple negative breast cancer cells by targeting STAT3

doi: 10.3892/mmr.2019.10044

Figure Lengend Snippet: Restoration of STAT3 impairs the prohibitive effects of miR-124 on cell viability and invasion in MDA-MB-468. (A) miR-124 expression levels in MDA-MB-468 cells infected with LV-miR-124. (B) Stable expression of miR-124 suppressed the expression of STAT3. However, STAT3 mRNA and protein levels were restored by transfection with plasmid-STAT3. Data values represent the relative intensity value. (C) STAT3 overexpression reversed the prohibitive cell viability caused by LV-miR-124. (D) A significantly suppressed invasive capacity was detected in MDA-MB-468 cells subsequent to LV-miR-124 transfection. This was partially recovered following plasmid-STAT3 transfection. All data are presented as mean ± standard deviation of at least three independent experiments. **P<0.01 vs. control. STAT3, signal transducer and activator of transcription 3; miR-124, microRNA-124; LV-miR-124, miR-124 expression lentivirus; plasmid-STAT3, STAT3 expression plasmid; NC, negative control.

Article Snippet: miRNA-124 mimics (miR-124), miRNA-124 inhibitor (inh-124), STAT3 overexpression plasmid [pcDNA3.0-STAT3 (plasmid-STAT3)] and corresponding negative controls [miR-NC, inh-NC and pcDNA3.0-empty vector (plasmid-NC)] were designed and purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China).

Techniques: Expressing, Infection, Transfection, Plasmid Preparation, Over Expression, Standard Deviation, Control, Negative Control

Journal: Molecular Medicine Reports

Article Title: MicroRNA-124 suppresses cell proliferation and invasion of triple negative breast cancer cells by targeting STAT3

doi: 10.3892/mmr.2019.10044

Figure Lengend Snippet: Transfection efficiency of plasmid-STAT3 and LV-si-STAT3 in MDA-MB-468 cells. (A) The transfection efficiency of plasmid-STAT3 was measured using RT-qPCR assays and western blot analysis. (B) RT-qPCR assays and western blot analysis were performed to evaluate the transfection efficiency of LV-si-STAT3. All data are presented as mean ± standard deviation of at least three independent experiments. Data values included in the western blot gel images represent the relative intensity value of the bands. **P<0.01 vs. control. STAT3, signal transducer and activator of transcription 3; siRNA, small interfering RNA; LV-si-STAT3, si-STAT3 lentivirus; RT-qPCR, reverse transcription quantitative polymerase chain reaction.

Article Snippet: miRNA-124 mimics (miR-124), miRNA-124 inhibitor (inh-124), STAT3 overexpression plasmid [pcDNA3.0-STAT3 (plasmid-STAT3)] and corresponding negative controls [miR-NC, inh-NC and pcDNA3.0-empty vector (plasmid-NC)] were designed and purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China).

Techniques: Transfection, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Standard Deviation, Control, Small Interfering RNA, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: MicroRNA-124 suppresses cell proliferation and invasion of triple negative breast cancer cells by targeting STAT3

doi: 10.3892/mmr.2019.10044

Figure Lengend Snippet: Overexpression of miR-124 and downregulation of STAT3 lead to smaller tumor sizes in vivo . (A) The proliferation curves of MDA-MB-468 cells following stable transfection of miR-124 or LV-si-STAT3 were obtained after 5 days of observation. (B) The representative nude mice and corresponding tumors for the four groups at 3 weeks post-inoculation. *P<0.05 and **P<0.01 vs. control. miR-124, microRNA-124; STAT3, signal transducer and activator of transcription 3; siRNA, small interfering RNA; LV-si-STAT3, si-STAT3 lentivirus.

Article Snippet: miRNA-124 mimics (miR-124), miRNA-124 inhibitor (inh-124), STAT3 overexpression plasmid [pcDNA3.0-STAT3 (plasmid-STAT3)] and corresponding negative controls [miR-NC, inh-NC and pcDNA3.0-empty vector (plasmid-NC)] were designed and purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China).

Techniques: Over Expression, In Vivo, Stable Transfection, Control, Small Interfering RNA

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Loss of epidermal MCPIP1 is associated with aggressive squamous cell carcinoma

doi: 10.1186/s13046-021-02202-3

Figure Lengend Snippet: The expression level of MCPIP1 negatively correlates with the expression levels of the genes encoding SERPINB3/B4 and MMP-9. ( a ) qRT-PCR analysis of Serpinb3a, -3b, -3c, -3d and Mmp9 expression in papillomas. n = 8-10 . ( b ) qRT-PCR analysis of Serpinb3a and -3b expression levels in whole skin lysates of 3-month-old and 6-month-old mice. n = 3-5 . ( c ) qRT-PCR analysis of SERPINB3, -B4 and MMP9 expression in A431 cells expressing shRNA against MCPIP1. n = 3/5. ( d ) qRT-PCR analysis of SERPINB3/B4 and MMP9 expression in A431 cells overexpressing MCPIP1 and treated with TPA for 24 h. n = 3. ( e ) Relative luciferase activity in HEK293 cells transfected with a luciferase reporter plasmid containing the 3’ - UTRs of SERPINB3/B4 and MMP9 or the pmirGLO-empty vector together with the control plasmid or the expression plasmid expressing wild-type (WT) MCPIP1 or the D141N mutant. Luciferase activity was normalized to that of the pmirGLO-empty vector. n = 4. The data are shown as the mean ± SD values. Unpaired t-test (A-B) or one-way ANOVA (D-E) was used to calculate P-values . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: HEK293 cells were seeded in 48-well plates one day before cotransfection with 282 ng of the pmirGLO-3’UTR or pmirGLO-empty vector and 18 ng of pcDNA3.0, pcDNA3.0-MCPIP1 (encoding human MCPIP1) or pcDNA3.0-MCPIP1-D141N using jetPRIME Reagent (Promega, Madison, USA).

Techniques: Expressing, Quantitative RT-PCR, shRNA, Luciferase, Activity Assay, Transfection, Plasmid Preparation, Control, Mutagenesis