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    Mirus Bio lipid based transfection reagent
    Downregulation of torsinA inhibits retro-translocation of the cholera toxin A1-chain (a) Immunoblot showing > 95% suppression of torsinA expression in COS cells treated with siRNA 1958 or 1963 for torsinA, with scr. siRNA as control. (b c): (b) After siRNA <t>transfection</t> cells were intoxicated with wild-type CT for 45 min at 37°C and then fractionated into cytosolic and membrane components which were resolved by SDS-PAGE. Immunoblotting was carried out with antibodies to CTA1, CTB, BiP, β-actin and Hsp90. (c) Bar graph showing relative band densities of CTA1 in the cytosol normalized to Hsp90 in the cytosol, and CTA1 in the membrane fraction normalized to BiP. Results are shown as the mean of 3 experiments ± S.D.; * represents p
    Lipid Based Transfection Reagent, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 88/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent/product/Mirus Bio
    Average 88 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent - by Bioz Stars, 2020-11
    88/100 stars
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    93
    Mirus Bio transit 2020
    Downregulation of torsinA inhibits retro-translocation of the cholera toxin A1-chain (a) Immunoblot showing > 95% suppression of torsinA expression in COS cells treated with siRNA 1958 or 1963 for torsinA, with scr. siRNA as control. (b c): (b) After siRNA <t>transfection</t> cells were intoxicated with wild-type CT for 45 min at 37°C and then fractionated into cytosolic and membrane components which were resolved by SDS-PAGE. Immunoblotting was carried out with antibodies to CTA1, CTB, BiP, β-actin and Hsp90. (c) Bar graph showing relative band densities of CTA1 in the cytosol normalized to Hsp90 in the cytosol, and CTA1 in the membrane fraction normalized to BiP. Results are shown as the mean of 3 experiments ± S.D.; * represents p
    Transit 2020, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 93/100, based on 1142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transit 2020/product/Mirus Bio
    Average 93 stars, based on 1142 article reviews
    Price from $9.99 to $1999.99
    transit 2020 - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

    Image Search Results


    Downregulation of torsinA inhibits retro-translocation of the cholera toxin A1-chain (a) Immunoblot showing > 95% suppression of torsinA expression in COS cells treated with siRNA 1958 or 1963 for torsinA, with scr. siRNA as control. (b c): (b) After siRNA transfection cells were intoxicated with wild-type CT for 45 min at 37°C and then fractionated into cytosolic and membrane components which were resolved by SDS-PAGE. Immunoblotting was carried out with antibodies to CTA1, CTB, BiP, β-actin and Hsp90. (c) Bar graph showing relative band densities of CTA1 in the cytosol normalized to Hsp90 in the cytosol, and CTA1 in the membrane fraction normalized to BiP. Results are shown as the mean of 3 experiments ± S.D.; * represents p

    Journal: Nature communications

    Article Title: TorsinA participates in endoplasmic reticulum-associated degradation

    doi: 10.1038/ncomms1383

    Figure Lengend Snippet: Downregulation of torsinA inhibits retro-translocation of the cholera toxin A1-chain (a) Immunoblot showing > 95% suppression of torsinA expression in COS cells treated with siRNA 1958 or 1963 for torsinA, with scr. siRNA as control. (b c): (b) After siRNA transfection cells were intoxicated with wild-type CT for 45 min at 37°C and then fractionated into cytosolic and membrane components which were resolved by SDS-PAGE. Immunoblotting was carried out with antibodies to CTA1, CTB, BiP, β-actin and Hsp90. (c) Bar graph showing relative band densities of CTA1 in the cytosol normalized to Hsp90 in the cytosol, and CTA1 in the membrane fraction normalized to BiP. Results are shown as the mean of 3 experiments ± S.D.; * represents p

    Article Snippet: After 24 h the cells were transfected with expression cassettes for GFP-CFTRΔF508 or pcDNA (negative control) and pcDNA-torsinAwt using a lipid-based transfection reagent: TransIT-2020, following the protocol of the manufacturer (Mirus Bio, WI).

    Techniques: Translocation Assay, Expressing, Transfection, SDS Page, CtB Assay

    Involvement of torsinA in degradation of GFP-CFTRΔF508 (a) 293T cells transfected with an expression cassette for GFP-CFTRΔF508 and 48 h later cell lysates were immunoprecipitated with IgG or antibodies to CFTR or torsinA, followed by SDS-PAGE and immunoblotting for GFP and torsinA. (b c): (b) 293T cells were co-transfected with expression cassettes for GFP-CFTRΔF508 or torsinA or torsinAΔE or torsinAΔC or with the control cassette, pcDNA 3.1. Twenty-four h later samples were treated or not with MG132 for 16 h. Seventy-two h post-transfection, cell lysates were processed by SDS-PAGE and gels immunoblotted with antibodies to GFP, torsinA and β-actin. Representative immunoblots are shown with short (1 min) and long (3 min) exposures for GFP, and with 3 min exposure for torsinA and β-actin. (c) GFP-CFTRΔF508 band densities were normalized to those of torsinA and β-actin and represented as the mean of 3 experiments ± S.D. For statistical comparisons, * denotes the comparisons of control to torsinA or torsinAΔE or torsinAΔC transfections (p,

    Journal: Nature communications

    Article Title: TorsinA participates in endoplasmic reticulum-associated degradation

    doi: 10.1038/ncomms1383

    Figure Lengend Snippet: Involvement of torsinA in degradation of GFP-CFTRΔF508 (a) 293T cells transfected with an expression cassette for GFP-CFTRΔF508 and 48 h later cell lysates were immunoprecipitated with IgG or antibodies to CFTR or torsinA, followed by SDS-PAGE and immunoblotting for GFP and torsinA. (b c): (b) 293T cells were co-transfected with expression cassettes for GFP-CFTRΔF508 or torsinA or torsinAΔE or torsinAΔC or with the control cassette, pcDNA 3.1. Twenty-four h later samples were treated or not with MG132 for 16 h. Seventy-two h post-transfection, cell lysates were processed by SDS-PAGE and gels immunoblotted with antibodies to GFP, torsinA and β-actin. Representative immunoblots are shown with short (1 min) and long (3 min) exposures for GFP, and with 3 min exposure for torsinA and β-actin. (c) GFP-CFTRΔF508 band densities were normalized to those of torsinA and β-actin and represented as the mean of 3 experiments ± S.D. For statistical comparisons, * denotes the comparisons of control to torsinA or torsinAΔE or torsinAΔC transfections (p,

    Article Snippet: After 24 h the cells were transfected with expression cassettes for GFP-CFTRΔF508 or pcDNA (negative control) and pcDNA-torsinAwt using a lipid-based transfection reagent: TransIT-2020, following the protocol of the manufacturer (Mirus Bio, WI).

    Techniques: Transfection, Expressing, Immunoprecipitation, SDS Page, Western Blot