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  • 90
    Thermo Fisher pcdna dest47
    Pcdna Dest47, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Addgene inc pcdna
    Effects of <t>everolimus,</t> Ku0063794, and their combination on the expression of SIRT2 in HepG2 cells ( A ) Western blot analyses showing the effects of everolimus (left), Ku0063794 (middle), and their combination (right) on SIRT1 expression in HepG2 cells. Although individual monotherapies could not inhibit SIRT1 expression, the combination therapy significantly inhibited SIRT1 expression in a dose-dependent manner. ( B ) Western blot analysis showing successful generation of SIRT1-overexpressing HepG2 cells by transfecting <t>pcDNA-SIRT1</t> into HepG2 cells. ( C ) [Left] Western blot analyses showing the expression of EMT markers both in normal and SIRT1-overexpressing HepG2 cells. [Right] Relative densities of these markers were quantified using Image J software. The combination therapy significantly increased the expression of E-cadherin and decreased the expression of Snail in the control HepG2 cells; however, the combination therapy could not inhibit EMT in SIRT1-overexpressing HepG2 cells. These results suggest that the combination therapy inhibits EMT of HepG2 cells by way of inhibiting SIRT1. ( D ) [Left] Immunofluorescence of E-cadherin (Top) and Snail (Bottom) in normal and SIRT1-overexpressing HepG2 cells (magnification × 400). [Right] Relative densities of these markers quantified using Image J software. The combination therapy significantly increased the expression of E-cadherin and decreased the expression of Snail in the control HepG2 cells; however, the combination therapy could not inhibit EMT in SIRT1-overexpressing HepG2 cells. These results also suggest that the combination therapy inhibits EMT of HepG2 cells by way of inhibiting SIRT1. Each data point represents the mean ± SD of three independent experiments. * P
    Pcdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcdna
    Dominant-negative <t>PID</t> expression inhibits cell viability and PAK phosphorylation in human MM cell lines. A) Immunoblot analysis of P-PAK1/2/3, total PAK1/2/3 and β-actin levels 72-hr post-nucleofection of <t>pcDNA</t> or pcDNA-PID in Me12 and Meso 22
    Pcdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna
    Co-culture of NSCs with Notch1-expressing cells or Dll1-expressing cells. (a) NSCs were cultured for 2 days on monolayers of Dll1 (2) or Notch1 (3) over-expressing COS7 cells and immunostained with Tuj1, a marker of neurons (red) and the NSC marker Nestin (green). (1): Control experiment with <t>pCDNA-transfected</t> COS7 cells. (b) NSCs were co-cultured for two (black bar: 2 days diff.) or four (white bar: 4 days diff.) days as described above. After immunostaining, Tuj1-positive neurons and Nestin-positive NSCs were counted. Values are means ± S.D. for 10 independent fields.*** P
    Pcdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher pcdna to
    Co-culture of NSCs with Notch1-expressing cells or Dll1-expressing cells. (a) NSCs were cultured for 2 days on monolayers of Dll1 (2) or Notch1 (3) over-expressing COS7 cells and immunostained with Tuj1, a marker of neurons (red) and the NSC marker Nestin (green). (1): Control experiment with <t>pCDNA-transfected</t> COS7 cells. (b) NSCs were co-cultured for two (black bar: 2 days diff.) or four (white bar: 4 days diff.) days as described above. After immunostaining, Tuj1-positive neurons and Nestin-positive NSCs were counted. Values are means ± S.D. for 10 independent fields.*** P
    Pcdna To, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene pcdna
    Co-culture of NSCs with Notch1-expressing cells or Dll1-expressing cells. (a) NSCs were cultured for 2 days on monolayers of Dll1 (2) or Notch1 (3) over-expressing COS7 cells and immunostained with Tuj1, a marker of neurons (red) and the NSC marker Nestin (green). (1): Control experiment with <t>pCDNA-transfected</t> COS7 cells. (b) NSCs were co-cultured for two (black bar: 2 days diff.) or four (white bar: 4 days diff.) days as described above. After immunostaining, Tuj1-positive neurons and Nestin-positive NSCs were counted. Values are means ± S.D. for 10 independent fields.*** P
    Pcdna, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcdna
    Co-culture of NSCs with Notch1-expressing cells or Dll1-expressing cells. (a) NSCs were cultured for 2 days on monolayers of Dll1 (2) or Notch1 (3) over-expressing COS7 cells and immunostained with Tuj1, a marker of neurons (red) and the NSC marker Nestin (green). (1): Control experiment with <t>pCDNA-transfected</t> COS7 cells. (b) NSCs were co-cultured for two (black bar: 2 days diff.) or four (white bar: 4 days diff.) days as described above. After immunostaining, Tuj1-positive neurons and Nestin-positive NSCs were counted. Values are means ± S.D. for 10 independent fields.*** P
    Pcdna, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna dest40
    Calibration EYFP-53BP1 reporter system. ( A ) EYFP-53BP1 primary structures. EYFP coding sequence is joined to full-length 53BP1, with Tudor and BRCT domains indicated. EYFP1-53BP coding sequence was inserted into <t>pcDNA-DEST40</t> (Invitrogen) for expression under control of the CMV promoter. ( B ) Dose response to calibrated doses of 137 Cs reference radiation. Cells were co-transfected with EYFP-53BP1 WT and H2B-diHcRed. Live-cell images were collected 30 min post-irradiation. ( C ) Quantification of dose response. Foci were scored using 8–24 individual nuclei at each dose point, and linear regression was performed to obtain the slope of the dose-response curve.
    Pcdna Dest40, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna dest53
    Calibration EYFP-53BP1 reporter system. ( A ) EYFP-53BP1 primary structures. EYFP coding sequence is joined to full-length 53BP1, with Tudor and BRCT domains indicated. EYFP1-53BP coding sequence was inserted into <t>pcDNA-DEST40</t> (Invitrogen) for expression under control of the CMV promoter. ( B ) Dose response to calibrated doses of 137 Cs reference radiation. Cells were co-transfected with EYFP-53BP1 WT and H2B-diHcRed. Live-cell images were collected 30 min post-irradiation. ( C ) Quantification of dose response. Foci were scored using 8–24 individual nuclei at each dose point, and linear regression was performed to obtain the slope of the dose-response curve.
    Pcdna Dest53, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna 3
    ORF 57 increases ORF 59/58 mRNA and protein levels. (A) Top, autoradiogram of a Northern blot containing total RNA from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 0.1 μg of pcDNA 3.1 ORF 57. A ds DNA probe specific for ORF 59 was used; bottom, the blot was reprobed with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific probe as a loading control. (B) Western blot analysis of protein extracts from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 1 μg of pcDNA 3.1 ORF 57. The ORF 59 protein was detected with a KSHV MAb, and then with a secondary goat anti-mouse conjugated to horseradish peroxidase and ECL (Amersham). Equal amounts (10 μg) of extracts as determined by Bradford assay from each transfection were loaded onto the gel.
    Pcdna 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore pcdna 3xflag
    ORF 57 increases ORF 59/58 mRNA and protein levels. (A) Top, autoradiogram of a Northern blot containing total RNA from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 0.1 μg of pcDNA 3.1 ORF 57. A ds DNA probe specific for ORF 59 was used; bottom, the blot was reprobed with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific probe as a loading control. (B) Western blot analysis of protein extracts from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 1 μg of pcDNA 3.1 ORF 57. The ORF 59 protein was detected with a KSHV MAb, and then with a secondary goat anti-mouse conjugated to horseradish peroxidase and ECL (Amersham). Equal amounts (10 μg) of extracts as determined by Bradford assay from each transfection were loaded onto the gel.
    Pcdna 3xflag, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna 4
    ORF 57 increases ORF 59/58 mRNA and protein levels. (A) Top, autoradiogram of a Northern blot containing total RNA from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 0.1 μg of pcDNA 3.1 ORF 57. A ds DNA probe specific for ORF 59 was used; bottom, the blot was reprobed with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific probe as a loading control. (B) Western blot analysis of protein extracts from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 1 μg of pcDNA 3.1 ORF 57. The ORF 59 protein was detected with a KSHV MAb, and then with a secondary goat anti-mouse conjugated to horseradish peroxidase and ECL (Amersham). Equal amounts (10 μg) of extracts as determined by Bradford assay from each transfection were loaded onto the gel.
    Pcdna 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IDEX Health & Science pcdna 4mtd3cpv
    ORF 57 increases ORF 59/58 mRNA and protein levels. (A) Top, autoradiogram of a Northern blot containing total RNA from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 0.1 μg of pcDNA 3.1 ORF 57. A ds DNA probe specific for ORF 59 was used; bottom, the blot was reprobed with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific probe as a loading control. (B) Western blot analysis of protein extracts from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 1 μg of pcDNA 3.1 ORF 57. The ORF 59 protein was detected with a KSHV MAb, and then with a secondary goat anti-mouse conjugated to horseradish peroxidase and ECL (Amersham). Equal amounts (10 μg) of extracts as determined by Bradford assay from each transfection were loaded onto the gel.
    Pcdna 4mtd3cpv, supplied by IDEX Health & Science, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GenePharma Company pcdna aeg1
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Aeg1, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc pcdna egfp
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna flag
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Flag, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore pcdna flagx3
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Flagx3, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna i
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    OriGene pcdna kynu
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Kynu, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc pcdna sadb19p
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Sadb19p, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GenePharma Company pcdna tp53inp1
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Tp53inp1, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc pcdna d3cpv
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna D3cpv, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna gw
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Gw, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of everolimus, Ku0063794, and their combination on the expression of SIRT2 in HepG2 cells ( A ) Western blot analyses showing the effects of everolimus (left), Ku0063794 (middle), and their combination (right) on SIRT1 expression in HepG2 cells. Although individual monotherapies could not inhibit SIRT1 expression, the combination therapy significantly inhibited SIRT1 expression in a dose-dependent manner. ( B ) Western blot analysis showing successful generation of SIRT1-overexpressing HepG2 cells by transfecting pcDNA-SIRT1 into HepG2 cells. ( C ) [Left] Western blot analyses showing the expression of EMT markers both in normal and SIRT1-overexpressing HepG2 cells. [Right] Relative densities of these markers were quantified using Image J software. The combination therapy significantly increased the expression of E-cadherin and decreased the expression of Snail in the control HepG2 cells; however, the combination therapy could not inhibit EMT in SIRT1-overexpressing HepG2 cells. These results suggest that the combination therapy inhibits EMT of HepG2 cells by way of inhibiting SIRT1. ( D ) [Left] Immunofluorescence of E-cadherin (Top) and Snail (Bottom) in normal and SIRT1-overexpressing HepG2 cells (magnification × 400). [Right] Relative densities of these markers quantified using Image J software. The combination therapy significantly increased the expression of E-cadherin and decreased the expression of Snail in the control HepG2 cells; however, the combination therapy could not inhibit EMT in SIRT1-overexpressing HepG2 cells. These results also suggest that the combination therapy inhibits EMT of HepG2 cells by way of inhibiting SIRT1. Each data point represents the mean ± SD of three independent experiments. * P

    Journal: Oncotarget

    Article Title: Potentiation of the anticancer effects of everolimus using a dual mTORC1/2 inhibitor in hepatocellular carcinoma cells

    doi: 10.18632/oncotarget.13808

    Figure Lengend Snippet: Effects of everolimus, Ku0063794, and their combination on the expression of SIRT2 in HepG2 cells ( A ) Western blot analyses showing the effects of everolimus (left), Ku0063794 (middle), and their combination (right) on SIRT1 expression in HepG2 cells. Although individual monotherapies could not inhibit SIRT1 expression, the combination therapy significantly inhibited SIRT1 expression in a dose-dependent manner. ( B ) Western blot analysis showing successful generation of SIRT1-overexpressing HepG2 cells by transfecting pcDNA-SIRT1 into HepG2 cells. ( C ) [Left] Western blot analyses showing the expression of EMT markers both in normal and SIRT1-overexpressing HepG2 cells. [Right] Relative densities of these markers were quantified using Image J software. The combination therapy significantly increased the expression of E-cadherin and decreased the expression of Snail in the control HepG2 cells; however, the combination therapy could not inhibit EMT in SIRT1-overexpressing HepG2 cells. These results suggest that the combination therapy inhibits EMT of HepG2 cells by way of inhibiting SIRT1. ( D ) [Left] Immunofluorescence of E-cadherin (Top) and Snail (Bottom) in normal and SIRT1-overexpressing HepG2 cells (magnification × 400). [Right] Relative densities of these markers quantified using Image J software. The combination therapy significantly increased the expression of E-cadherin and decreased the expression of Snail in the control HepG2 cells; however, the combination therapy could not inhibit EMT in SIRT1-overexpressing HepG2 cells. These results also suggest that the combination therapy inhibits EMT of HepG2 cells by way of inhibiting SIRT1. Each data point represents the mean ± SD of three independent experiments. * P

    Article Snippet: Chemicals and reagents Everolimus and Ku0063794 were obtained from Selleckchem (Farmingdale, NY), and pcDNA and pcDNA-SIRT1 were obtained from Addgene (Cambridge, MA).

    Techniques: Expressing, Western Blot, Software, Immunofluorescence

    Dominant-negative PID expression inhibits cell viability and PAK phosphorylation in human MM cell lines. A) Immunoblot analysis of P-PAK1/2/3, total PAK1/2/3 and β-actin levels 72-hr post-nucleofection of pcDNA or pcDNA-PID in Me12 and Meso 22

    Journal: Molecular cancer research : MCR

    Article Title: Group I p21-Activated Kinases (PAKs) Promote Tumor Cell Proliferation and Survival Through the AKT1 and Raf-MAPK Pathways

    doi: 10.1158/1541-7786.MCR-12-0082

    Figure Lengend Snippet: Dominant-negative PID expression inhibits cell viability and PAK phosphorylation in human MM cell lines. A) Immunoblot analysis of P-PAK1/2/3, total PAK1/2/3 and β-actin levels 72-hr post-nucleofection of pcDNA or pcDNA-PID in Me12 and Meso 22

    Article Snippet: A third human MM line (Meso-17) was transfected with pcDNA or pcDNA-PID vectors (20 μg/transfection) using the Xfect™ Transfection Reagent (Clontech).

    Techniques: Dominant Negative Mutation, Expressing

    Co-culture of NSCs with Notch1-expressing cells or Dll1-expressing cells. (a) NSCs were cultured for 2 days on monolayers of Dll1 (2) or Notch1 (3) over-expressing COS7 cells and immunostained with Tuj1, a marker of neurons (red) and the NSC marker Nestin (green). (1): Control experiment with pCDNA-transfected COS7 cells. (b) NSCs were co-cultured for two (black bar: 2 days diff.) or four (white bar: 4 days diff.) days as described above. After immunostaining, Tuj1-positive neurons and Nestin-positive NSCs were counted. Values are means ± S.D. for 10 independent fields.*** P

    Journal: Nucleic Acids Research

    Article Title: The Delta intracellular domain mediates TGF-?/Activin signaling through binding to Smads and has an important bi-directional function in the Notch-Delta signaling pathway

    doi: 10.1093/nar/gkl1128

    Figure Lengend Snippet: Co-culture of NSCs with Notch1-expressing cells or Dll1-expressing cells. (a) NSCs were cultured for 2 days on monolayers of Dll1 (2) or Notch1 (3) over-expressing COS7 cells and immunostained with Tuj1, a marker of neurons (red) and the NSC marker Nestin (green). (1): Control experiment with pCDNA-transfected COS7 cells. (b) NSCs were co-cultured for two (black bar: 2 days diff.) or four (white bar: 4 days diff.) days as described above. After immunostaining, Tuj1-positive neurons and Nestin-positive NSCs were counted. Values are means ± S.D. for 10 independent fields.*** P

    Article Snippet: Cells were also transfected with pCDNA (Invitrogen) as a negative control.

    Techniques: Co-Culture Assay, Expressing, Cell Culture, Marker, Transfection, Immunostaining

    Overexpression of calpain small subunit 1 (Capn4) reversed the inhibitory effect of miR-124 on the Wnt/β-catenin signaling pathway. HONE1 and CNE2 cells were transfected with miR-124 or co-transfected with miR-124 and pcDNA-Capn4 ( A and B ). Western blot analysis revealed that Capn4 overexpression reversed the decreased β-catenin, cyclin D1, and c-Myc protein levels caused by the overexpression of miR-124 in HONE1 and CNE2 cells. Data are shown as mean ± standard deviation (n=3). * P

    Journal: OncoTargets and therapy

    Article Title: miR-124 suppresses proliferation and invasion of nasopharyngeal carcinoma cells through the Wnt/β-catenin signaling pathway by targeting Capn4

    doi: 10.2147/OTT.S135563

    Figure Lengend Snippet: Overexpression of calpain small subunit 1 (Capn4) reversed the inhibitory effect of miR-124 on the Wnt/β-catenin signaling pathway. HONE1 and CNE2 cells were transfected with miR-124 or co-transfected with miR-124 and pcDNA-Capn4 ( A and B ). Western blot analysis revealed that Capn4 overexpression reversed the decreased β-catenin, cyclin D1, and c-Myc protein levels caused by the overexpression of miR-124 in HONE1 and CNE2 cells. Data are shown as mean ± standard deviation (n=3). * P

    Article Snippet: Cell transfection The pcDNA-Capn4 and pcDNA-control were purchased from Thermo Fisher Scientific.

    Techniques: Over Expression, Transfection, Western Blot, Standard Deviation

    MicroRNA-124 (miR-124) suppressed proliferation and invasion of NPC cells through the inhibition of Capn4 expression. HONE1 and CNE2 cells were transfected with miR-124 or in combination with pcDNA-Capn4. Notes: ( A ) MTT assay confirmed that restored Capn4 expression reversed the inhibitory effect of miR-124 on the cell viability of HONE1 and CNE2 cells at 24, 48, and 72 h after transfection. Data are shown as mean ± standard error of mean (n=3) ( B and C ) Transwell invasion assay suggested that transfection of pcDNA-Capn4 abolished miR-124-mediated inhibition of cell invasion in HONE1 and CNE2 cells. Data are shown as mean ± standard deviation (n=3). * P

    Journal: OncoTargets and therapy

    Article Title: miR-124 suppresses proliferation and invasion of nasopharyngeal carcinoma cells through the Wnt/β-catenin signaling pathway by targeting Capn4

    doi: 10.2147/OTT.S135563

    Figure Lengend Snippet: MicroRNA-124 (miR-124) suppressed proliferation and invasion of NPC cells through the inhibition of Capn4 expression. HONE1 and CNE2 cells were transfected with miR-124 or in combination with pcDNA-Capn4. Notes: ( A ) MTT assay confirmed that restored Capn4 expression reversed the inhibitory effect of miR-124 on the cell viability of HONE1 and CNE2 cells at 24, 48, and 72 h after transfection. Data are shown as mean ± standard error of mean (n=3) ( B and C ) Transwell invasion assay suggested that transfection of pcDNA-Capn4 abolished miR-124-mediated inhibition of cell invasion in HONE1 and CNE2 cells. Data are shown as mean ± standard deviation (n=3). * P

    Article Snippet: Cell transfection The pcDNA-Capn4 and pcDNA-control were purchased from Thermo Fisher Scientific.

    Techniques: Inhibition, Expressing, Transfection, MTT Assay, Transwell Invasion Assay, Standard Deviation

    Calibration EYFP-53BP1 reporter system. ( A ) EYFP-53BP1 primary structures. EYFP coding sequence is joined to full-length 53BP1, with Tudor and BRCT domains indicated. EYFP1-53BP coding sequence was inserted into pcDNA-DEST40 (Invitrogen) for expression under control of the CMV promoter. ( B ) Dose response to calibrated doses of 137 Cs reference radiation. Cells were co-transfected with EYFP-53BP1 WT and H2B-diHcRed. Live-cell images were collected 30 min post-irradiation. ( C ) Quantification of dose response. Foci were scored using 8–24 individual nuclei at each dose point, and linear regression was performed to obtain the slope of the dose-response curve.

    Journal: Nucleic Acids Research

    Article Title: Use of a microscope stage-mounted Nickel-63 microirradiator for real-time observation of the DNA double-strand break response

    doi: 10.1093/nar/gkq409

    Figure Lengend Snippet: Calibration EYFP-53BP1 reporter system. ( A ) EYFP-53BP1 primary structures. EYFP coding sequence is joined to full-length 53BP1, with Tudor and BRCT domains indicated. EYFP1-53BP coding sequence was inserted into pcDNA-DEST40 (Invitrogen) for expression under control of the CMV promoter. ( B ) Dose response to calibrated doses of 137 Cs reference radiation. Cells were co-transfected with EYFP-53BP1 WT and H2B-diHcRed. Live-cell images were collected 30 min post-irradiation. ( C ) Quantification of dose response. Foci were scored using 8–24 individual nuclei at each dose point, and linear regression was performed to obtain the slope of the dose-response curve.

    Article Snippet: The resulting construct was transferred using phage lambda recombinase into pcDNA-DEST40 (Invitrogen) for mammalian expression under the control of the CMV promoter/enhancer.

    Techniques: Sequencing, Expressing, Transfection, Irradiation

    ORF 57 increases ORF 59/58 mRNA and protein levels. (A) Top, autoradiogram of a Northern blot containing total RNA from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 0.1 μg of pcDNA 3.1 ORF 57. A ds DNA probe specific for ORF 59 was used; bottom, the blot was reprobed with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific probe as a loading control. (B) Western blot analysis of protein extracts from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 1 μg of pcDNA 3.1 ORF 57. The ORF 59 protein was detected with a KSHV MAb, and then with a secondary goat anti-mouse conjugated to horseradish peroxidase and ECL (Amersham). Equal amounts (10 μg) of extracts as determined by Bradford assay from each transfection were loaded onto the gel.

    Journal: Journal of Virology

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 57 Encodes a Posttranscriptional Regulator with Multiple Distinct Activities †

    doi:

    Figure Lengend Snippet: ORF 57 increases ORF 59/58 mRNA and protein levels. (A) Top, autoradiogram of a Northern blot containing total RNA from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 0.1 μg of pcDNA 3.1 ORF 57. A ds DNA probe specific for ORF 59 was used; bottom, the blot was reprobed with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific probe as a loading control. (B) Western blot analysis of protein extracts from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 59/58 in the presence (+) or absence (−) of 1 μg of pcDNA 3.1 ORF 57. The ORF 59 protein was detected with a KSHV MAb, and then with a secondary goat anti-mouse conjugated to horseradish peroxidase and ECL (Amersham). Equal amounts (10 μg) of extracts as determined by Bradford assay from each transfection were loaded onto the gel.

    Article Snippet: The resulting fragment was cloned into the Hin dIII and Eco RV sites of pcDNA 3 (Invitrogen) to create pcDNA 3 nut-1.

    Techniques: Northern Blot, Transfection, Western Blot, Bradford Assay

    ). Forty-eight hours posttransfection, luciferase activity was measured and values were plotted that represented fold activation relative to that of ORF 50 alone, which was set to 1. (C) Mutations in the nut-1 promoter abolish synergy between ORF 50 and ORF 57. CV-1 cells were transfected with 2 μg of pcDNA 3 ORF 50, increasing amounts of ORF 57, and either 1 μg of the wild-type nut-1-706 reporter or the nut-1-706 mutant reporter. Forty-eight hours posttransfection, luciferase activity was measured and values were plotted that represent fold activation relative to that of ORF 50 alone, which was set to 1.

    Journal: Journal of Virology

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 57 Encodes a Posttranscriptional Regulator with Multiple Distinct Activities †

    doi:

    Figure Lengend Snippet: ). Forty-eight hours posttransfection, luciferase activity was measured and values were plotted that represented fold activation relative to that of ORF 50 alone, which was set to 1. (C) Mutations in the nut-1 promoter abolish synergy between ORF 50 and ORF 57. CV-1 cells were transfected with 2 μg of pcDNA 3 ORF 50, increasing amounts of ORF 57, and either 1 μg of the wild-type nut-1-706 reporter or the nut-1-706 mutant reporter. Forty-eight hours posttransfection, luciferase activity was measured and values were plotted that represent fold activation relative to that of ORF 50 alone, which was set to 1.

    Article Snippet: The resulting fragment was cloned into the Hin dIII and Eco RV sites of pcDNA 3 (Invitrogen) to create pcDNA 3 nut-1.

    Techniques: Luciferase, Activity Assay, Activation Assay, Transfection, Mutagenesis

    The C-terminally tagged version of ORF 57 is localized to the nucleus. The panels show results of immunofluorescence staining of CV-1 cells 48 h after transfection with either empty pcDNA 3.1-V5 vector (left panel) or pcDNA 3.1-V5 ORF 57 (right panel). Reactivity to anti-V5 antibody (Invitrogen) was detected with a TRITC-conjugated rabbit anti-mouse antibody.

    Journal: Journal of Virology

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 57 Encodes a Posttranscriptional Regulator with Multiple Distinct Activities †

    doi:

    Figure Lengend Snippet: The C-terminally tagged version of ORF 57 is localized to the nucleus. The panels show results of immunofluorescence staining of CV-1 cells 48 h after transfection with either empty pcDNA 3.1-V5 vector (left panel) or pcDNA 3.1-V5 ORF 57 (right panel). Reactivity to anti-V5 antibody (Invitrogen) was detected with a TRITC-conjugated rabbit anti-mouse antibody.

    Article Snippet: The resulting fragment was cloned into the Hin dIII and Eco RV sites of pcDNA 3 (Invitrogen) to create pcDNA 3 nut-1.

    Techniques: Immunofluorescence, Staining, Transfection, Plasmid Preparation

    ORF 50 mRNA and protein levels are not significantly increased by ORF 57 expression. (A) Top, autoradiogram of a Northern blot containing total RNA from CV-1 cells transfected with a fixed amount of pcDNA 3 ORF 50 and the following amounts of pcDNA 3.1 ORF 57: 0 μg (lane 1), 0.75 μg (lane 2), 2.5 μg (lane 3), and 7.5 μg (lane 4). An antisense riboprobe for ORF 50 was hybridized to the blot. Bottom, the blot was reprobed with a GAPDH-specific probe as a loading control. (B) Western blot analysis of protein extracts from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 50 and the following amounts of pcDNA 3.1 ORF 57: 0 μg (lane 1), 0.5 μg (lane 2), 2.5 μg (lane 3), and 5 μg (lane 4). The ORF 50 protein was detected with a polyclonal antibody and then with a secondary rabbit anti-mouse antibody conjugated to horseradish peroxidase and ECL (Amersham). Equal amount of extracts as determined by Bradford assay from each transfection were loaded onto the gel.

    Journal: Journal of Virology

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 57 Encodes a Posttranscriptional Regulator with Multiple Distinct Activities †

    doi:

    Figure Lengend Snippet: ORF 50 mRNA and protein levels are not significantly increased by ORF 57 expression. (A) Top, autoradiogram of a Northern blot containing total RNA from CV-1 cells transfected with a fixed amount of pcDNA 3 ORF 50 and the following amounts of pcDNA 3.1 ORF 57: 0 μg (lane 1), 0.75 μg (lane 2), 2.5 μg (lane 3), and 7.5 μg (lane 4). An antisense riboprobe for ORF 50 was hybridized to the blot. Bottom, the blot was reprobed with a GAPDH-specific probe as a loading control. (B) Western blot analysis of protein extracts from CV-1 cells transfected with a fixed amount of pcDNA 3.1 ORF 50 and the following amounts of pcDNA 3.1 ORF 57: 0 μg (lane 1), 0.5 μg (lane 2), 2.5 μg (lane 3), and 5 μg (lane 4). The ORF 50 protein was detected with a polyclonal antibody and then with a secondary rabbit anti-mouse antibody conjugated to horseradish peroxidase and ECL (Amersham). Equal amount of extracts as determined by Bradford assay from each transfection were loaded onto the gel.

    Article Snippet: The resulting fragment was cloned into the Hin dIII and Eco RV sites of pcDNA 3 (Invitrogen) to create pcDNA 3 nut-1.

    Techniques: Expressing, Northern Blot, Transfection, Western Blot, Bradford Assay

    Effect of introns on synergistic activation of different promoters by the ORF 50-ORF 57 combination. CV-1 cells were cotransfected with fixed amounts of the indicated KSHV promoter constructs with or without introns (Nut-1, TK, and Kaposin), a fixed amount of the transcriptional activator, pcDNA 3 ORF 50, and increasing amounts of pcDNA 3.1 ORF 57 (0, 0.3, 1, and 3 μg). Luciferase activity was measured 48 h after transfection. (A) Log scale graph displaying the effects on the Nut-1 promoter with and without an intron. (B) Graph displaying the effects on the Kaposin and TK promoters with and without an intron. Values are plotted as fold luciferase activity over that of ORF 50 alone, which was set to 1. Error bars representing standard deviations are from two experiments performed in duplicate. Note that activation is plotted on an arithmetic scale in B and on a log scale in A.

    Journal: Journal of Virology

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 57 Encodes a Posttranscriptional Regulator with Multiple Distinct Activities †

    doi:

    Figure Lengend Snippet: Effect of introns on synergistic activation of different promoters by the ORF 50-ORF 57 combination. CV-1 cells were cotransfected with fixed amounts of the indicated KSHV promoter constructs with or without introns (Nut-1, TK, and Kaposin), a fixed amount of the transcriptional activator, pcDNA 3 ORF 50, and increasing amounts of pcDNA 3.1 ORF 57 (0, 0.3, 1, and 3 μg). Luciferase activity was measured 48 h after transfection. (A) Log scale graph displaying the effects on the Nut-1 promoter with and without an intron. (B) Graph displaying the effects on the Kaposin and TK promoters with and without an intron. Values are plotted as fold luciferase activity over that of ORF 50 alone, which was set to 1. Error bars representing standard deviations are from two experiments performed in duplicate. Note that activation is plotted on an arithmetic scale in B and on a log scale in A.

    Article Snippet: The resulting fragment was cloned into the Hin dIII and Eco RV sites of pcDNA 3 (Invitrogen) to create pcDNA 3 nut-1.

    Techniques: Activation Assay, Construct, Luciferase, Activity Assay, Transfection

    Effect of ORF 57 expression on viral promoter constructs with and without introns. CV-1 cells were cotransfected with increasing amounts of pcDNA 3.1 ORF 57 (0, 0.3, 1, and 3 μg) and fixed amounts of the following KSHV promoter luciferase constructs with or without an intron: DNA Pol, Kaposin, Nut-1, and TK. Luciferase activity was measured 48 h posttransfection. Graphs are representative examples of experiments performed in duplicate. Error bars represent standard deviations.

    Journal: Journal of Virology

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 57 Encodes a Posttranscriptional Regulator with Multiple Distinct Activities †

    doi:

    Figure Lengend Snippet: Effect of ORF 57 expression on viral promoter constructs with and without introns. CV-1 cells were cotransfected with increasing amounts of pcDNA 3.1 ORF 57 (0, 0.3, 1, and 3 μg) and fixed amounts of the following KSHV promoter luciferase constructs with or without an intron: DNA Pol, Kaposin, Nut-1, and TK. Luciferase activity was measured 48 h posttransfection. Graphs are representative examples of experiments performed in duplicate. Error bars represent standard deviations.

    Article Snippet: The resulting fragment was cloned into the Hin dIII and Eco RV sites of pcDNA 3 (Invitrogen) to create pcDNA 3 nut-1.

    Techniques: Expressing, Construct, Luciferase, Activity Assay

    Upregulation of nut-1 RNA accumulation by ORF 57 expression. The indicated constructs (bottom) were transfected into CV-1 cells in the presence (+) or absence (−) of 1 μg of pcDNA 3.1 ORF 57. Forty-eight hours later, total RNA was prepared from whole-cell lysates, electrophoresed through 1% agarose-formaldehyde gels, transferred to Hybond-N+ membranes, and hybridized to probes specific for nut-1 (A) or ORF 74/GCR (B, left panel) or ORF K5 (B, right panel). Below each panel is shown rRNA bands in ethidium bromide-stained lanes as a loading control.

    Journal: Journal of Virology

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 57 Encodes a Posttranscriptional Regulator with Multiple Distinct Activities †

    doi:

    Figure Lengend Snippet: Upregulation of nut-1 RNA accumulation by ORF 57 expression. The indicated constructs (bottom) were transfected into CV-1 cells in the presence (+) or absence (−) of 1 μg of pcDNA 3.1 ORF 57. Forty-eight hours later, total RNA was prepared from whole-cell lysates, electrophoresed through 1% agarose-formaldehyde gels, transferred to Hybond-N+ membranes, and hybridized to probes specific for nut-1 (A) or ORF 74/GCR (B, left panel) or ORF K5 (B, right panel). Below each panel is shown rRNA bands in ethidium bromide-stained lanes as a loading control.

    Article Snippet: The resulting fragment was cloned into the Hin dIII and Eco RV sites of pcDNA 3 (Invitrogen) to create pcDNA 3 nut-1.

    Techniques: Expressing, Construct, Transfection, Staining

    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Article Snippet: miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Expressing, Cotransfection

    The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p

    Article Snippet: miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Expressing, Transfection, Cotransfection

    miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p

    Article Snippet: miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Cotransfection, Activity Assay, Transfection, Migration