Journal: Molecular nutrition & food research

Article Title: Flavone Hispidulin Stimulates Glucagon-Like peptide-1 secretion and Ameliorates Hyperglycemia in Streptozotocin-Induced Diabetic Mice

doi: 10.1002/mnfr.201900978

Figure Lengend Snippet: (A) Healthy male C57BL/6J mice (10 wks old) was fasted 5h and then given 1 g/kg glucose plus 20 mg/kg hispidulin dissolved in 2% methylcellulose or 1 g/kg glucose plus vehicle (Control) via oral gavage. Blood samples were collected at 0 and 20 min after oral gavage, and plasma total GLP-1 levels were measured (n= 8 mice/group). A second cohort of male mice (9 wks old) was fasted 5 h and then given 20 mg/kg hispidulin or vehicle (Control) via oral gavage. Food intake was measured after 1, 3, 5, 8, 12, and 24 h of oral gavage (n=10 mice/group). STZ-induced diabetic mice were administered with hispidulin (20 mg/kg via oral gavage) or vehicle for 6 wks. Body weight (C) and non-fasting (D) and fasting (E) blood glucose levels were measured at indicated time points during hispidulin treatment. Glucose tolerance test were performed after 5-wk treatment (F), and the area under the curve was calculated shown on the right panel of F. GSIS (G) and non-fasting plasma insulin levels (H) were measured at the end of feeding experiment; livers were isolated and pyruvate carboxylase (PCB) protein levels in whole cell lysates were measured by immunoblotting and normalized to β-action contents (I). Data are means ± SEM (n=11–13 mice/group). (J) Freshly isolated hepatocytes from healthy male mice (10 wks old) were cultured in gluconeogenic medium containing various concentrations of hispidulin or forskolin (F: 10 μM) for 3 h. Glucose production was determined using an assay kit. Data are means ± SEM from 4 independent experiments. C: non-diabetic mice; STZ: STZ-induced diabetic mice treated with vehicle; STZ+H: STZ-induced diabetic mice treated with hispidulin. * P < 0.05, ** P < 0.01, and *** P <0.001 vs. C; # P < 0.05, ### P <0.001, and #### P <0.0001 vs. STZ alone.

Article Snippet: Pyruvate carboxylase (PCB) antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas).

Techniques: Control, Clinical Proteomics, Isolation, Western Blot, Cell Culture

Journal: Molecules

Article Title: Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture

doi: 10.3390/molecules25030709

Figure Lengend Snippet: Residual PCB congeners content in sterile Soil A contaminated with PCBs inoculated by Mycolicibacterium frederiksbergense IN53, Rhodococcus erythropolis IN129, Rhodococcus sp. IN306 and mixed culture M1 after a 30-day biodegradation process, (repetition number n = 8–10, p < 0.05). Control-non-inoculated Soil A.

Article Snippet: The identification and quantitative analyses of 18 PCB congeners (PCB28 (2,4,4′-), PCB52 (2,2′,5,5′-), PCB77 (3,3′,4,4′-), PCB81 (3,4,4′,5-), PCB101 (2,2′,4,5,5′-), PCB105 (2,3,3′,4,4′-), PCB114 (2,3,4,4′,5-), PCB118 (2,3′,4,4′,5-), PCB123 (2,3′,4,4′,5′-), PCB126 (3,3′,4,4′,5-), PCB138 (2,2′,3,4,4′,5′-), PCB 153 (2,2′,4,4′,5,5′-), PCB156 (2,3,3′,4,4′,5-), PCB157 (2,3,3′,4,4′,5-), PCB167 (2,3′,4,4′,5,5′-), PCB169 (3,3′,4,4′,5,5′-), PCB180 (2,2′,3,4,4′,5,5′-), and PCB189 (2,3,3′,4,4′,5,5′-) were carried out using a certified reference material namely: PCB congeners in the SQC068-50g soil (Sigma-Aldrich, USA).

Techniques: Sterility, Control

Journal: Molecules

Article Title: Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture

doi: 10.3390/molecules25030709

Figure Lengend Snippet: Residual content of ( a ) PCB congeners and ( b ) n-alkanes in non-sterile soil contaminated with both PCBs and petroleum hydrocarbons (Soil D) after 2 (Soil D2), 4 (Soils D4) and 6 (Soil D6) months of inoculation with mixed culture M1 (ex-situ prism method), (repetition number n = 7–10, p < 0.05). Control non-inoculated Soil D.

Article Snippet: The identification and quantitative analyses of 18 PCB congeners (PCB28 (2,4,4′-), PCB52 (2,2′,5,5′-), PCB77 (3,3′,4,4′-), PCB81 (3,4,4′,5-), PCB101 (2,2′,4,5,5′-), PCB105 (2,3,3′,4,4′-), PCB114 (2,3,4,4′,5-), PCB118 (2,3′,4,4′,5-), PCB123 (2,3′,4,4′,5′-), PCB126 (3,3′,4,4′,5-), PCB138 (2,2′,3,4,4′,5′-), PCB 153 (2,2′,4,4′,5,5′-), PCB156 (2,3,3′,4,4′,5-), PCB157 (2,3,3′,4,4′,5-), PCB167 (2,3′,4,4′,5,5′-), PCB169 (3,3′,4,4′,5,5′-), PCB180 (2,2′,3,4,4′,5,5′-), and PCB189 (2,3,3′,4,4′,5,5′-) were carried out using a certified reference material namely: PCB congeners in the SQC068-50g soil (Sigma-Aldrich, USA).

Techniques: Sterility, Ex Situ, Control

Journal: Optics Express

Article Title: Subwavelength 3D terahertz imaging with a single-pixel laser transceiver

doi: 10.1364/oe.561713

Figure Lengend Snippet: Fig. 9. LFI tomography imaging of a packaged chip. (a) LFI THz amplitude and phase images of a PCB. (b) LFI THz peak-to-peak tomography imaging of a PCB (first row), LFI THz amplitude (second row) and phase tomography imaging (third row) of one of the PCB’s packaged chips (Texas Instrument LMH6629SD). The bottom row consists of reduction images, where amplitude and phase information were combined using the HSV color space (phase for hue, amplitude for value).

Article Snippet: LFI tomography imaging of a packaged chip. (a) LFI THz amplitude and phase images of a PCB. (b) LFI THz peak-to-peak tomography imaging of a PCB (first row), LFI THz amplitude (second row) and phase tomography imaging (third row) of one of the PCB’s packaged chips (Texas Instrument LMH6629SD).

Techniques: Tomography, Imaging

Journal: The Journal of Neuroscience

Article Title: Phenotypic Alteration of a Human BK ( hSlo ) Channel by hSloβ Subunit Coexpression: Changes in Blocker Sensitivity, Activation/Relaxation and Inactivation Kinetics, and Protein Kinase A Modulation

doi: 10.1523/JNEUROSCI.16-15-04543.1996

Figure Lengend Snippet: IBTX and ChTX pharmacology. A, Application of the BK channel-blocking peptide IbTX to oocytes expressing hSlo or hSlo + hSloβ revealed that coexpression with the hSloβ subunit resulted in a nearly 10-fold decrease in the sensitivity to IbTX blockade;n = 5–10 oocytes/IbTX concentration. Maximal effect was defined as the effect produced by incubation in 100–250 nm IbTX; maximal levels of suppression did not differ significantly between hSlo and hSlo + hSloβ. B, Application of the peptidyl blocker ChTX did not reveal a significantly different profile of blockade forhSlo and hSlo + hSloβ. Maximal effect was defined as the response to 250–500 nmChTX, and maximal levels of effect did not differ betweenhSlo and hSlo + hSloβ.

Article Snippet: Iberiotoxin (IbTX) and charybdotoxin (ChTX) (Peptides International, Louisville, KY) were prepared as aqueous stocks and diluted in MBS before use; tetrandrine (Aldrich, Milwaukee, WI) was prepared as a stock solution in dimethylformamide or dimethylsulfoxide (DMSO) and likewise diluted in the appropriate solution just before use.

Techniques: Blocking Assay, Expressing, Concentration Assay, Produced, Incubation