Journal: Cell Death Discovery

Article Title: TRAIL DR5-CTSB crosstalk participates in breast cancer autophagy initiated by SAHA

doi: 10.1038/cddiscovery.2017.52

Figure Lengend Snippet: The effects of SAHA and TRAIL DR5 on the expressions of autophagy-related genes in cancer cells. RNA was isolated from MDA-MB-231 or MCF-7 cells using high pure RNA isolation kit following manufacturer’s instructions. To obtain the first-strand cDNA, transcriptor first strand cDNA synthesis kit was used and cDNA was as a template in real-time PCR reactions with power SYBR green PCR master mix. Exprofile human autophagy Gene qPCR array kit was employed to describe related mRNA expression. Relative changes of gene expression in the array were calculated using the 2 −ΔΔCt (threshold cycle) method. ( a – c ). MDA-MB-231 cells, ( d – f ). MCF-7 cells, ( g ). Related data analysis. Relative quantitative values represent mean±S.E.M.; * represents statistical significance of P <0.05 comparing with Basal, ** represents statistical significance of P <0.01 comparing with Basal.

Article Snippet: Exprofile human autophagy gene qPCR array kit was obtained from Genecopoeia (Rockville, MD.

Techniques: Isolation, cDNA Synthesis, Real-time Polymerase Chain Reaction, SYBR Green Assay, Expressing, Gene Expression