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ATCC
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Addgene inc
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ATCC
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Addgene inc
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ABclonal Biotechnology
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Biosynth Carbosynth
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ATCC
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Image Search Results
Journal: Development (Cambridge, England)
Article Title: A novel floor plate boundary defined by adjacent En1 and Dbx1 microdomains distinguishes midbrain dopamine and hypothalamic neurons
doi: 10.1242/dev.144949
Figure Lengend Snippet: DA neuron markers are expressed in ventral premammillary neurons. (A) Allen Brain Atlas schematic showing regions of interest. (B,C) P30 Slc6a3::Cre, Ai9 sections immunolabeled with DA markers Foxa2, Lmx1a, Nr4a2 and Th (arrows indicate co-labeled cells). Only few PMv neurons (up to two per section) were Th+ (arrowheads). HY, hypothalamus; PMd, dorsal premammillary nucleus; PMv, ventral premammillary nucleus; ZI, zona incerta. Scale bars:100 µm (B); 25 µm (C).
Article Snippet: To generate the En1OE transgene,
Techniques: Immunolabeling, Labeling
Journal: Applied microbiology and biotechnology
Article Title: Study on the framework of ATP energy cycle system in Escherichia coli.
doi: 10.1007/s00253-024-13350-9
Figure Lengend Snippet: Fig.4 The location and ATP synthesis activity of strains (IS186, IS186 + 8array). a The location and ATP synthesis activity of strains (IS186, IS186 + 8array). b Identification of IS186 + 8Array locus inser- tion. c Identification of ATP synthesis activity
Article Snippet: pQCascade-IS1 (addgene # 140,624), pCutamp (addgene #140,632), pTnsABC (addgene #160,031), pDonor-GDH (addgene #140,629) and pQCascade-X plasmids were obtained through pQCascade-IS1 modification, including pQCascade-IS186 (addgene #140,627), pQCascade-8array (original name pQCascade array1, addgene #140,623), and
Techniques: Activity Assay
Journal: Applied microbiology and biotechnology
Article Title: Study on the framework of ATP energy cycle system in Escherichia coli.
doi: 10.1007/s00253-024-13350-9
Figure Lengend Snippet: Fig. 5 The changes in Nrk, Pnk, and ATP synthesis activity, and expression levels. a The changes of Nrk and ATP syn- thesis activity. b The changes of Pnk and ATP synthesis activity. c The changes of expression levels of one plasmid contains two genes: CK, BL21 (DE3). 1, IS186-ATP5; 2, pET29a- Pnk-RBS-ATP; 3, pET29a-Pnk (IS186-ATP5) Clone 1; 4, pET29a-Pnk (IS186-ATP5) Clone 2; 5, pET29a-Nrk-RBS- ATP; 6–8, pET29a-Nrk (IS186- ATP5) Clones 1–3
Article Snippet: pQCascade-IS1 (addgene # 140,624), pCutamp (addgene #140,632), pTnsABC (addgene #160,031), pDonor-GDH (addgene #140,629) and pQCascade-X plasmids were obtained through pQCascade-IS1 modification, including pQCascade-IS186 (addgene #140,627), pQCascade-8array (original name pQCascade array1, addgene #140,623), and
Techniques: Activity Assay, Expressing, Plasmid Preparation, Clone Assay
Journal: Open Life Sciences
Article Title: Bone marrow stromal cells enhance multiple myeloma cells proliferation through regulating LncRNA OVAAL/ENPP1 axis
doi: 10.1515/biol-2025-1322
Figure Lengend Snippet: LncRNA data analysis of RNA sequencing. (A) Sample correlation analysis. (B) Heatmap clustering of differentially expressed lncRNAs between U266 cells and co-cultured system. (C) Volcano plot of differentially expressed lncRNAs between U266 cells and co-cultured system. (D) Venn diagram of differentially expressed lncRNAs targeting UPB1, ENPP3, and ENPP1. (E) The specific differentially expressed lncRNAs concurrently targeting UPB1, ENPP3, and ENPP1. (F) The relative expression of OVAAL, FOXD3-AS1, and LINCO1235 in U266 cells and co-cultured system detected using RNA sequencing. ** p < 0.01; *** p < 0.001; **** p < 0.0001.
Article Snippet: After blocking with 5 % skim milk (2 h), membranes were incubated overnight at 4 °C with primary
Techniques: RNA Sequencing, Cell Culture, Expressing
Journal: Open Life Sciences
Article Title: Bone marrow stromal cells enhance multiple myeloma cells proliferation through regulating LncRNA OVAAL/ENPP1 axis
doi: 10.1515/biol-2025-1322
Figure Lengend Snippet: Clinical bone marrow mononuclear cells samples were employed to detect the relative expression of OVAAL, FOXD3-AS1, LINCO1235, UPB1, ENPP3, and ENPP1 in MM patients and healthy controls. * p < 0.05; ** p < 0.01; ns, no significant.
Article Snippet: After blocking with 5 % skim milk (2 h), membranes were incubated overnight at 4 °C with primary
Techniques: Expressing
Journal: Open Life Sciences
Article Title: Bone marrow stromal cells enhance multiple myeloma cells proliferation through regulating LncRNA OVAAL/ENPP1 axis
doi: 10.1515/biol-2025-1322
Figure Lengend Snippet: The relative expression of OVAAL, UPB1, ENPP3, and ENPP1 were validated in U266 cells and co-cultured system. * p < 0.05; *** p < 0.001; **** p < 0.0001.
Article Snippet: After blocking with 5 % skim milk (2 h), membranes were incubated overnight at 4 °C with primary
Techniques: Expressing, Cell Culture
Journal: Open Life Sciences
Article Title: Bone marrow stromal cells enhance multiple myeloma cells proliferation through regulating LncRNA OVAAL/ENPP1 axis
doi: 10.1515/biol-2025-1322
Figure Lengend Snippet: The interference of OVAAL expression results in ENPP1 expression suppression. (A) The relative expression of OVAAL detection using RT-qPCR. (B) The relative expression of ENPP1 detection using RT-qPCR. (C) The expression of ENPP1 protein detection using western blot. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, no significant.
Article Snippet: After blocking with 5 % skim milk (2 h), membranes were incubated overnight at 4 °C with primary
Techniques: Expressing, Quantitative RT-PCR, Western Blot