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    4 Paraformaldehyde solution in PBS is a ready to use solution for sample preparation and fixing cells for immunohistochemistry IHC
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    Rabbit Polyclonal Rtf1 Antibody
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    Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Phosphate Buffered Saline Pbs Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 613 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Phosphate Buffered Saline Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 690 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore propidium iodide pi
    The LeishIF4E2(+/-) deletion mutant shows altered promastigote morphology. (A) Mid-log phase (Day 2) promastigotes of wild type, Cas9/T7 expressers, LeishIF4E2(+/-) cells, and LeishIF4E2 add-back cells were fixed with 2% paraformaldehyde and visualized by phase contrast microscopy at 100x magnification. Wild type (WT), Cas9/T7 expressing cells showed normal promastigote morphology while LeishIF4E2(+/-) became round with reduced flagellar length. This altered phenotype was reverted back to normal in LeishIF4E2 add-back cells. (B) All the cell lines were stained with 20 µg/mL <t>propidium</t> iodide (PI) for 30 minutes and cell viability was analyzed using the ImageStream X Mark II Imaging flow cytometer (Millipore). 20,000 cells were analyzed for each sample and percent viable cells were determined. (C) .The circularity of single, viable and focused cells from each of the cell lines was quantified using flow cytometry and shown as percentage of the total number of cells measured. Data from three independent experiments are shown.
    Propidium Iodide Pi, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pbs phosphate buffered saline 10x ph 7 4
    The LeishIF4E2(+/-) deletion mutant shows altered promastigote morphology. (A) Mid-log phase (Day 2) promastigotes of wild type, Cas9/T7 expressers, LeishIF4E2(+/-) cells, and LeishIF4E2 add-back cells were fixed with 2% paraformaldehyde and visualized by phase contrast microscopy at 100x magnification. Wild type (WT), Cas9/T7 expressing cells showed normal promastigote morphology while LeishIF4E2(+/-) became round with reduced flagellar length. This altered phenotype was reverted back to normal in LeishIF4E2 add-back cells. (B) All the cell lines were stained with 20 µg/mL <t>propidium</t> iodide (PI) for 30 minutes and cell viability was analyzed using the ImageStream X Mark II Imaging flow cytometer (Millipore). 20,000 cells were analyzed for each sample and percent viable cells were determined. (C) .The circularity of single, viable and focused cells from each of the cell lines was quantified using flow cytometry and shown as percentage of the total number of cells measured. Data from three independent experiments are shown.
    Pbs Phosphate Buffered Saline 10x Ph 7 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Thrombomodulin BDCA 3 Antibody RTM98 from Novus Biologicals is a rat monoclonal antibody to Thrombomodulin BDCA 3 This antibody reacts with human The Thrombomodulin BDCA 3 Antibody RTM98 has
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    PBS Stock Solution 10X pH 7 4 at 1X contains filtered concentrated PBS It is ready to use as supplied
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    Image Search Results


    The LeishIF4E2(+/-) deletion mutant shows altered promastigote morphology. (A) Mid-log phase (Day 2) promastigotes of wild type, Cas9/T7 expressers, LeishIF4E2(+/-) cells, and LeishIF4E2 add-back cells were fixed with 2% paraformaldehyde and visualized by phase contrast microscopy at 100x magnification. Wild type (WT), Cas9/T7 expressing cells showed normal promastigote morphology while LeishIF4E2(+/-) became round with reduced flagellar length. This altered phenotype was reverted back to normal in LeishIF4E2 add-back cells. (B) All the cell lines were stained with 20 µg/mL propidium iodide (PI) for 30 minutes and cell viability was analyzed using the ImageStream X Mark II Imaging flow cytometer (Millipore). 20,000 cells were analyzed for each sample and percent viable cells were determined. (C) .The circularity of single, viable and focused cells from each of the cell lines was quantified using flow cytometry and shown as percentage of the total number of cells measured. Data from three independent experiments are shown.

    Journal: bioRxiv

    Article Title: Distinct features of the Leishmania cap-binding protein LeishIF4E2 revealed by CRISPR-Cas9 mediated heterozygous deletion

    doi: 10.1101/2020.05.13.093914

    Figure Lengend Snippet: The LeishIF4E2(+/-) deletion mutant shows altered promastigote morphology. (A) Mid-log phase (Day 2) promastigotes of wild type, Cas9/T7 expressers, LeishIF4E2(+/-) cells, and LeishIF4E2 add-back cells were fixed with 2% paraformaldehyde and visualized by phase contrast microscopy at 100x magnification. Wild type (WT), Cas9/T7 expressing cells showed normal promastigote morphology while LeishIF4E2(+/-) became round with reduced flagellar length. This altered phenotype was reverted back to normal in LeishIF4E2 add-back cells. (B) All the cell lines were stained with 20 µg/mL propidium iodide (PI) for 30 minutes and cell viability was analyzed using the ImageStream X Mark II Imaging flow cytometer (Millipore). 20,000 cells were analyzed for each sample and percent viable cells were determined. (C) .The circularity of single, viable and focused cells from each of the cell lines was quantified using flow cytometry and shown as percentage of the total number of cells measured. Data from three independent experiments are shown.

    Article Snippet: Flow cytometry analysis of LeishmaniaCell viability was verified by incubation of the cells with 20 µg/mL propidium iodide (PI) during 30 minutes.

    Techniques: Mutagenesis, Microscopy, Expressing, Staining, Imaging, Flow Cytometry