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  • 99
    Thermo Fisher phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Thermo Fisher
    Average 99 stars, based on 460 article reviews
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    99
    Bio-Rad phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Bio-Rad
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    96
    Cell Signaling Technology Inc phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Cell Signaling Technology Inc
    Average 96 stars, based on 21 article reviews
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    99
    Boster Bio phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/GE Healthcare
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    90
    Merck KGaA phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nissui Pharmaceutical phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Nissui Pharmaceutical
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    92
    PAA Laboratories phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    BioMed Diagnostics Inc phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Phosphate Buffered Saline Pbs Solution, supplied by BioMed Diagnostics Inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher phosphate buffered solution pbs
    Comparison of the Raman spectra of D. discoideum growth and starvation <t>EVs.</t> (A) Proposed interpretation of Raman spectrum originated from vesicles released from D. discoideum in the starvation phase. This spectrum was obtained by averaging 15 different sets of vesicles, with a total accumulation time of 14 minutes, then subtraction of the <t>PBS</t> contribution, and smoothing by 2 adjacent pixels. (B) Variability of Raman spectra of D. discoideum vesicles during growth (a, b) as compared to starvation phase (c, d). Within the same phase, one can qualitatively distinguish at least 2 different species: during growth, species (b) is characterised by an increased amount of lipids as compared to species (a), and during starvation, species (d) contains an increased amount of nucleic acids and carotenoids as compared to species (c). The prominent spectral differences are highlighted by dashed oval curves.
    Phosphate Buffered Solution Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphate buffered solution pbs - by Bioz Stars, 2020-07
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    92
    Biochrom phosphate buffered saline pbs solution
    Comparison of the Raman spectra of D. discoideum growth and starvation <t>EVs.</t> (A) Proposed interpretation of Raman spectrum originated from vesicles released from D. discoideum in the starvation phase. This spectrum was obtained by averaging 15 different sets of vesicles, with a total accumulation time of 14 minutes, then subtraction of the <t>PBS</t> contribution, and smoothing by 2 adjacent pixels. (B) Variability of Raman spectra of D. discoideum vesicles during growth (a, b) as compared to starvation phase (c, d). Within the same phase, one can qualitatively distinguish at least 2 different species: during growth, species (b) is characterised by an increased amount of lipids as compared to species (a), and during starvation, species (d) contains an increased amount of nucleic acids and carotenoids as compared to species (c). The prominent spectral differences are highlighted by dashed oval curves.
    Phosphate Buffered Saline Pbs Solution, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Biochrom
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    93
    Fisher Scientific phosphate buffered saline pbs solution
    Comparison of the Raman spectra of D. discoideum growth and starvation <t>EVs.</t> (A) Proposed interpretation of Raman spectrum originated from vesicles released from D. discoideum in the starvation phase. This spectrum was obtained by averaging 15 different sets of vesicles, with a total accumulation time of 14 minutes, then subtraction of the <t>PBS</t> contribution, and smoothing by 2 adjacent pixels. (B) Variability of Raman spectra of D. discoideum vesicles during growth (a, b) as compared to starvation phase (c, d). Within the same phase, one can qualitatively distinguish at least 2 different species: during growth, species (b) is characterised by an increased amount of lipids as compared to species (a), and during starvation, species (d) contains an increased amount of nucleic acids and carotenoids as compared to species (c). The prominent spectral differences are highlighted by dashed oval curves.
    Phosphate Buffered Saline Pbs Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher pbs solution
    Workflow of sample processing and movement of cells in the FCMC device. (A) Workflow of sample processing and CTC detection. (a) Blood sample collection and enrichment using Ficoll-Paque PLUS. (b) The cell suspension is loaded into the FCMC device. The cells form monolayers in the microchambers and are immunostained by using a syringe pump. (c) All microchambers are scanned using a fluorescent microscope. (d) CTC are detected from the fluorescent images. (B) Movement of cells in the FCMC device. (a) The new CM chip, which is made from polystyrene (PS), is cleaned with UV ozone and coated with <t>BSA.</t> (b) The FCMC device is washed with <t>PBS.</t> (c) A cell suspension (68 uL) that is equivalent to 0.4 mL of blood is loaded into one FCMC device. (d) After “Suction for Trapping”, untrapped cells move into downstream microchambers and settle down on the bottom of these microchambers. The blue arrows indicate flow. (e) After “Suction for Monolayer”, overlapped cells are discharged from microchambers and move to the outside of microchambers or to the inside of downstream microchambers. (f) After repetitions of (d) and (e), all cells are trapped in a monolayer. (g), (h), (i) Immunostaining solutions are loaded, incubated and washed.
    Pbs Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2864 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in PBS, a low-molecular weight (357 kDa) HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).

    Journal: Nature communications

    Article Title: Inertio-elastic focusing of bioparticles in microchannels at high throughput

    doi: 10.1038/ncomms5120

    Figure Lengend Snippet: Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in PBS, a low-molecular weight (357 kDa) HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).

    Article Snippet: HA sodium salt (357 kDa (Lifecore Biomedical) and 1650 kDa (Sigma-Aldrich)) was added to water (Sigma-Aldrich) for bead suspensions or phosphate-buffered saline (PBS) solution (Life Technologies) solution for cell suspensions and prepared using a roller mixer (Stuart, Sigma-Aldrich).

    Techniques: Molecular Weight, Flow Cytometry

    Comparison of the Raman spectra of D. discoideum growth and starvation EVs. (A) Proposed interpretation of Raman spectrum originated from vesicles released from D. discoideum in the starvation phase. This spectrum was obtained by averaging 15 different sets of vesicles, with a total accumulation time of 14 minutes, then subtraction of the PBS contribution, and smoothing by 2 adjacent pixels. (B) Variability of Raman spectra of D. discoideum vesicles during growth (a, b) as compared to starvation phase (c, d). Within the same phase, one can qualitatively distinguish at least 2 different species: during growth, species (b) is characterised by an increased amount of lipids as compared to species (a), and during starvation, species (d) contains an increased amount of nucleic acids and carotenoids as compared to species (c). The prominent spectral differences are highlighted by dashed oval curves.

    Journal: Journal of Extracellular Vesicles

    Article Title: Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy

    doi: 10.3402/jev.v1i0.19179

    Figure Lengend Snippet: Comparison of the Raman spectra of D. discoideum growth and starvation EVs. (A) Proposed interpretation of Raman spectrum originated from vesicles released from D. discoideum in the starvation phase. This spectrum was obtained by averaging 15 different sets of vesicles, with a total accumulation time of 14 minutes, then subtraction of the PBS contribution, and smoothing by 2 adjacent pixels. (B) Variability of Raman spectra of D. discoideum vesicles during growth (a, b) as compared to starvation phase (c, d). Within the same phase, one can qualitatively distinguish at least 2 different species: during growth, species (b) is characterised by an increased amount of lipids as compared to species (a), and during starvation, species (d) contains an increased amount of nucleic acids and carotenoids as compared to species (c). The prominent spectral differences are highlighted by dashed oval curves.

    Article Snippet: EVs, present in the 12,000×g pellet, are resuspended in a phosphate-buffered solution (PBS) [pH 7.4 without calcium and magnesium (Gibco)], with a 20 to 100 volume concentration factor (X), relative to the volume of the 2,000×g supernatant.

    Techniques:

    Size distribution and concentration of D. discoideum EVs samples ( Table I ), as measured by NTA. EVs were prepared from growth medium after 48 hours of cell growth, either with the usual conditions for the 12,000×g centrifugation (in Eppendorf tubes) (a), or with the 12,000×g centrifugation performed in 30 ml tubes (see Materials and methods ) (b). The curve (c) corresponds to EVs remaining in the 12,000×g supernatant, which relates to sample (b) EVs. The 12,000×g pellets (a and b) are concentrated in PBS by factors of 20 and 100, respectively, whereas the 12,000×g supernatant (c) is as obtained after centrifugation.

    Journal: Journal of Extracellular Vesicles

    Article Title: Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy

    doi: 10.3402/jev.v1i0.19179

    Figure Lengend Snippet: Size distribution and concentration of D. discoideum EVs samples ( Table I ), as measured by NTA. EVs were prepared from growth medium after 48 hours of cell growth, either with the usual conditions for the 12,000×g centrifugation (in Eppendorf tubes) (a), or with the 12,000×g centrifugation performed in 30 ml tubes (see Materials and methods ) (b). The curve (c) corresponds to EVs remaining in the 12,000×g supernatant, which relates to sample (b) EVs. The 12,000×g pellets (a and b) are concentrated in PBS by factors of 20 and 100, respectively, whereas the 12,000×g supernatant (c) is as obtained after centrifugation.

    Article Snippet: EVs, present in the 12,000×g pellet, are resuspended in a phosphate-buffered solution (PBS) [pH 7.4 without calcium and magnesium (Gibco)], with a 20 to 100 volume concentration factor (X), relative to the volume of the 2,000×g supernatant.

    Techniques: Concentration Assay, Centrifugation

    Workflow of sample processing and movement of cells in the FCMC device. (A) Workflow of sample processing and CTC detection. (a) Blood sample collection and enrichment using Ficoll-Paque PLUS. (b) The cell suspension is loaded into the FCMC device. The cells form monolayers in the microchambers and are immunostained by using a syringe pump. (c) All microchambers are scanned using a fluorescent microscope. (d) CTC are detected from the fluorescent images. (B) Movement of cells in the FCMC device. (a) The new CM chip, which is made from polystyrene (PS), is cleaned with UV ozone and coated with BSA. (b) The FCMC device is washed with PBS. (c) A cell suspension (68 uL) that is equivalent to 0.4 mL of blood is loaded into one FCMC device. (d) After “Suction for Trapping”, untrapped cells move into downstream microchambers and settle down on the bottom of these microchambers. The blue arrows indicate flow. (e) After “Suction for Monolayer”, overlapped cells are discharged from microchambers and move to the outside of microchambers or to the inside of downstream microchambers. (f) After repetitions of (d) and (e), all cells are trapped in a monolayer. (g), (h), (i) Immunostaining solutions are loaded, incubated and washed.

    Journal: EBioMedicine

    Article Title: Prognostic Impact of Circulating Tumor Cell Detected Using a Novel Fluidic Cell Microarray Chip System in Patients with Breast Cancer

    doi: 10.1016/j.ebiom.2016.07.027

    Figure Lengend Snippet: Workflow of sample processing and movement of cells in the FCMC device. (A) Workflow of sample processing and CTC detection. (a) Blood sample collection and enrichment using Ficoll-Paque PLUS. (b) The cell suspension is loaded into the FCMC device. The cells form monolayers in the microchambers and are immunostained by using a syringe pump. (c) All microchambers are scanned using a fluorescent microscope. (d) CTC are detected from the fluorescent images. (B) Movement of cells in the FCMC device. (a) The new CM chip, which is made from polystyrene (PS), is cleaned with UV ozone and coated with BSA. (b) The FCMC device is washed with PBS. (c) A cell suspension (68 uL) that is equivalent to 0.4 mL of blood is loaded into one FCMC device. (d) After “Suction for Trapping”, untrapped cells move into downstream microchambers and settle down on the bottom of these microchambers. The blue arrows indicate flow. (e) After “Suction for Monolayer”, overlapped cells are discharged from microchambers and move to the outside of microchambers or to the inside of downstream microchambers. (f) After repetitions of (d) and (e), all cells are trapped in a monolayer. (g), (h), (i) Immunostaining solutions are loaded, incubated and washed.

    Article Snippet: The second immunostaining solution included 1:500 diluted Alexa Fluor 488 Goat Anti-Mouse IgG1 (γ1) (Life Technologies, Grand Island, NY), 1:500 diluted Alexa Fluor 647 Goat Anti-Mouse IgG2a (γ2a) (Life technologies) and 1:1000 diluted Hoechst 33342 10 mg/mL solution (Life technologies) in PBS solution containing 1% Tween 20 and 3% BSA.

    Techniques: Microscopy, Chromatin Immunoprecipitation, Flow Cytometry, Immunostaining, Incubation