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Cambridge Isotope Laboratories
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R&D Systems
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Immunotec inc
tsp1 p10 antibody ![]() Tsp1 P10 Antibody, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/pbr+buffer/monoclonal+rat+antihuman+tsp+1+antibody/us08491897-1016-9-13 Average 90 stars, based on 1 article reviews
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Cambridge Isotope Laboratories
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Cambridge Isotope Laboratories
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Cambridge Isotope Laboratories
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ICN Biomedicals
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Hilgenberg gmbh
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CH Instruments
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Porphyrin Systems GbR
5,10,15,20-tetrakis(4-sulfonatophenyl)-porphine pd:tspp ![]() 5,10,15,20 Tetrakis(4 Sulfonatophenyl) Porphine Pd:Tspp, supplied by Porphyrin Systems GbR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/pbr+buffer/5+10+15+20+tetrakis+4+sulfonatophenyl++21+23h+porphyrin/pm17346700-51-7-9 Average 90 stars, based on 1 article reviews
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Image Search Results
Journal:
Article Title: Neutralizing Antibodies Inhibit Axonal Spread of Herpes Simplex Virus Type 1 to Epidermal Cells In Vitro
doi:
Figure Lengend Snippet: Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min and permeabilized with 0.1% Triton X-100 (Sigma) in PBS for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Article Snippet: The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (
Techniques: Infection, Staining, Incubation, Microscopy