Journal: International journal of radiation biology

Article Title: Evaluation of continuous low dose rate versus acute single high dose rate radiation combined with oncolytic viral therapy for prostate cancer

doi: 10.3109/09553000903419338

Figure Lengend Snippet: In vitro cytotoxicity of CRAd in combination with two types of radiation. LNCaP cells were infected with 2 MOI of Ad5 PSE/PBN E1A-AR on 48-well plates for both combinations. In (a), acute single HDR radiation (6 Gy) was performed 24 hours prior to infection. In (b), continuous LDR radiation (6 Gy) was performed immediately after infection. MTT assay was used to assess cell viability. The enhanced cytotoxicity of the CRAd monotherapy in the LDR experiment, in comparison to the HDR experiment, is due to the difference in shorter time gap (24 hours vs. 48 hours) between cell plating and viral infection. Error bars indicate the standard error of the mean (SEM) for three independent experiments.

Article Snippet: Viruses and their dosage Ad5 PSE/PBN E1A-AR ( Hoti et al. 2007 ) was generated in the AdEasy system (a gift from Dr Bert Vogelstein laboratory at Johns Hopkins University School of Medicine) through recombination of line-arised shuttle plasmid RpS-PSE-PBN-E1A-AR (RpS: Rodriguez plasmid shuttle) with pAdEasy-1 in DPL-S11 cells.

Techniques: In Vitro, Infection, MTT Assay, Comparison

Journal: International journal of radiation biology

Article Title: Evaluation of continuous low dose rate versus acute single high dose rate radiation combined with oncolytic viral therapy for prostate cancer

doi: 10.3109/09553000903419338

Figure Lengend Snippet: Reciprocal effect of radiation on viral replication. In (a) and (c), LNCaP cells were infected with 2 MOI of Ad5 PSE/PBN E1A-AR together with 30 MOI of FFIG on 48-well plates. GFP level was measured using a fluorescent plate reader and the data was normalised to the percentage of surviving cells. In (b) and (d), LNCaP cells were infected with 2 MOI of Ad5 PSE/PBN E1A-AR on 100 mm dishes; the amount of viral amplification seven days after treatment was titered using antibody against viral hexon. In (a) & (b): acute single HDR radiation (6 Gy) was performed 24 hours prior to infection. In (c) & (d): continuous LDR radiation (6 Gy) was performed immediately after infection. Error bars indicate the SEM for three independent experiments.

Article Snippet: Viruses and their dosage Ad5 PSE/PBN E1A-AR ( Hoti et al. 2007 ) was generated in the AdEasy system (a gift from Dr Bert Vogelstein laboratory at Johns Hopkins University School of Medicine) through recombination of line-arised shuttle plasmid RpS-PSE-PBN-E1A-AR (RpS: Rodriguez plasmid shuttle) with pAdEasy-1 in DPL-S11 cells.

Techniques: Infection, Amplification

Journal: International journal of radiation biology

Article Title: Evaluation of continuous low dose rate versus acute single high dose rate radiation combined with oncolytic viral therapy for prostate cancer

doi: 10.3109/09553000903419338

Figure Lengend Snippet: Viral/acute single HDR radiation combination causes increased expression of double-stranded DNA damage marker γ-H2AX. LNCaP cells were infected with 2 MOI of Ad5 PSE/PBN E1A-AR on 100 mm dishes either 24 hours following acute single HDR radiation (6 Gy) (as control, un-irradiated cells were seeded and infected at the same time, denoted as a) or immediately followed by continuous LDR radiation (6 Gy) (as control, un-irradiated cells were seeded and infected at the same time, denoted as b). Whole cell extracts were collected seven days post-treatment. For Western blotting, 10–20 μg of whole cell extract was loaded on 4–15% acrylamide gels.

Article Snippet: Viruses and their dosage Ad5 PSE/PBN E1A-AR ( Hoti et al. 2007 ) was generated in the AdEasy system (a gift from Dr Bert Vogelstein laboratory at Johns Hopkins University School of Medicine) through recombination of line-arised shuttle plasmid RpS-PSE-PBN-E1A-AR (RpS: Rodriguez plasmid shuttle) with pAdEasy-1 in DPL-S11 cells.

Techniques: Expressing, Marker, Infection, Control, Irradiation, Western Blot