pbind-id Search Results


pbind-id  (Promega)
90
Promega pbind-id
Pbind, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbind-id/product/Promega
Average 90 stars, based on 1 article reviews
pbind-id - by Bioz Stars, 2026-06
90/100 stars
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pbind-id pact-myod control vectors  (Promega)
90
Promega pbind-id pact-myod control vectors
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Pbind Id Pact Myod Control Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbind-id pact-myod control vectors/product/Promega
Average 90 stars, based on 1 article reviews
pbind-id pact-myod control vectors - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
checkmate mammalian two-hybrid kit containing vectors pbind-id and pact-myod  (Promega)
90
Promega checkmate mammalian two-hybrid kit containing vectors pbind-id and pact-myod
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Checkmate Mammalian Two Hybrid Kit Containing Vectors Pbind Id And Pact Myod, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/checkmate mammalian two-hybrid kit containing vectors pbind-id and pact-myod/product/Promega
Average 90 stars, based on 1 article reviews
checkmate mammalian two-hybrid kit containing vectors pbind-id and pact-myod - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer (pBIND-Id and pACT-MyoD control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.

Journal:

Article Title: ATF6 Activated by Proteolysis Binds in the Presence of NF-Y (CBF) Directly to the cis -Acting Element Responsible for the Mammalian Unfolded Protein Response

doi:

Figure Lengend Snippet: Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer (pBIND-Id and pACT-MyoD control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.

Article Snippet: The positive control supplied by the manufacturer (pBIND-Id and pACT-MyoD control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.

Techniques: Construct, Transfection, Plasmid Preparation, Binding Assay, Luciferase, Positive Control, Activity Assay