pbind vectors Search Results


pact-vp16-n-cor(1–740)  (Promega)
90
Promega pact-vp16-n-cor(1–740)
Pact Vp16 N Cor(1–740), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbind renilla luciferase control vector  (Promega)
90
Promega pbind renilla luciferase control vector
Pbind Renilla Luciferase Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbind-id pact-myod control vectors  (Promega)
90
Promega pbind-id pact-myod control vectors
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Pbind Id Pact Myod Control Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbind-id pact-myod control vectors - by Bioz Stars, 2026-05
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plasmid dna of themammalian vectors pbind  (Promega)
90
Promega plasmid dna of themammalian vectors pbind
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Plasmid Dna Of Themammalian Vectors Pbind, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid dna of themammalian vectors pbind - by Bioz Stars, 2026-05
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pbind plasmid e1581  (Promega)
90
Promega pbind plasmid e1581
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Pbind Plasmid E1581, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbind plasmid e1581/product/Promega
Average 90 stars, based on 1 article reviews
pbind plasmid e1581 - by Bioz Stars, 2026-05
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checkmate mammalian two-hybrid kit containing vectors pbind-id and pact-myod  (Promega)
90
Promega checkmate mammalian two-hybrid kit containing vectors pbind-id and pact-myod
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Checkmate Mammalian Two Hybrid Kit Containing Vectors Pbind Id And Pact Myod, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/checkmate mammalian two-hybrid kit containing vectors pbind-id and pact-myod/product/Promega
Average 90 stars, based on 1 article reviews
checkmate mammalian two-hybrid kit containing vectors pbind-id and pact-myod - by Bioz Stars, 2026-05
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mammalian expression vectors pbind  (Promega)
90
Promega mammalian expression vectors pbind
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Mammalian Expression Vectors Pbind, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mammalian expression vectors pbind - by Bioz Stars, 2026-05
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pbind mammalian expression vector  (Promega)
90
Promega pbind mammalian expression vector
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Pbind Mammalian Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbind mammalian expression vector/product/Promega
Average 90 stars, based on 1 article reviews
pbind mammalian expression vector - by Bioz Stars, 2026-05
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mammalian two-hybrid vectors pbind  (Promega)
90
Promega mammalian two-hybrid vectors pbind
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Mammalian Two Hybrid Vectors Pbind, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mammalian two-hybrid vectors pbind/product/Promega
Average 90 stars, based on 1 article reviews
mammalian two-hybrid vectors pbind - by Bioz Stars, 2026-05
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expression vectors pbind  (Promega)
90
Promega expression vectors pbind
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Expression Vectors Pbind, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression vectors pbind/product/Promega
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expression vectors pbind - by Bioz Stars, 2026-05
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pbind-pu.1 vector  (Promega)
90
Promega pbind-pu.1 vector
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Pbind Pu.1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbind-pu.1 vector/product/Promega
Average 90 stars, based on 1 article reviews
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checkmate vectors pbind  (Promega)
90
Promega checkmate vectors pbind
Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer <t>(pBIND-Id</t> <t>and</t> <t>pACT-MyoD</t> control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.
Checkmate Vectors Pbind, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/checkmate vectors pbind/product/Promega
Average 90 stars, based on 1 article reviews
checkmate vectors pbind - by Bioz Stars, 2026-05
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Image Search Results


Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer (pBIND-Id and pACT-MyoD control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.

Journal:

Article Title: ATF6 Activated by Proteolysis Binds in the Presence of NF-Y (CBF) Directly to the cis -Acting Element Responsible for the Mammalian Unfolded Protein Response

doi:

Figure Lengend Snippet: Mapping of the transactivation domain of ATF6. (Left) Schematic structures of ATF6 as well as fusion proteins between GAL4DB and various ATF6 subregions. The dotted lines delineate the region deleted from the construct. The positions of the bZIP region and transmembrane domain (TMD) are indicated. (Right) Transcriptional activities of various fusion proteins. HeLa cells in 24-well plates were transiently transfected with each of the fusion plasmids together with the reporter plasmid pG5luc containing five Gal4p binding sites upstream of the firefly luciferase gene. Constitutively expressed luciferase activities were determined and normalized as described in Materials and Methods. Relative activities are presented as averages with standard deviations (error bars) from triplicate determinations of four independent transfections. The positive control supplied by the manufacturer (pBIND-Id and pACT-MyoD control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.

Article Snippet: The positive control supplied by the manufacturer (pBIND-Id and pACT-MyoD control vectors; Promega) showed a relative activity of 4.6 ± 0.3 in this assay.

Techniques: Construct, Transfection, Plasmid Preparation, Binding Assay, Luciferase, Positive Control, Activity Assay