Journal: Brain Research

Article Title: Establishment of a simplified in vitro porcine blood–brain barrier model with high transendothelial electrical resistance

doi: 10.1016/j.brainres.2012.06.057

Figure Lengend Snippet: Phase contrast images of a primary porcine brain endothelial cell (PBEC) culture. The cells were treated with 4 μg/ml puromycin for three days to remove contaminating cells (as described in ). Porcine brain endothelial cells start to migrate from microvessel fragments from day 1. By day 3, the culture is about 70% confluent and can be passaged at this stage. Bottom right image shows confluent P.1 PBEC cultures on Transwell inserts, three days after passaging (six days from thawing). Scale bar: 50 μm.

Article Snippet: We recently adopted a porcine brain endothelial cell (PBEC) model first developed at Eisai Laboratories (London) by Dr. Louise Morgan and colleagues, based on a successful earlier bovine brain endothelial cell model ( ).

Techniques: Passaging

Journal: Brain Research

Article Title: Establishment of a simplified in vitro porcine blood–brain barrier model with high transendothelial electrical resistance

doi: 10.1016/j.brainres.2012.06.057

Figure Lengend Snippet: Quality control panel used to assess porcine brain endothelial cells.

Article Snippet: We recently adopted a porcine brain endothelial cell (PBEC) model first developed at Eisai Laboratories (London) by Dr. Louise Morgan and colleagues, based on a successful earlier bovine brain endothelial cell model ( ).

Techniques: Expressing, Permeability

Journal: Brain Research

Article Title: Establishment of a simplified in vitro porcine blood–brain barrier model with high transendothelial electrical resistance

doi: 10.1016/j.brainres.2012.06.057

Figure Lengend Snippet: Comparison of relative mRNA expression levels of BCRP, occludin and claudin-5 in PBEC cultures. (A) Relative mRNA expression levels between batches of PBEC. The results are expressed as ‘fold difference’ ratio between batch 2 and batch 1 PBEC cultures (mean±SEM, n =6). (B) Relative mRNA expression levels between primary and P.1 PBEC cultures within each batch. Results are expressed as ‘fold difference’ ratio between passage 1 and primary PBEC cultures (mean±SEM, n =6). Statistical significance was determined by two-way ANOVA and showed no significant differences between batches or cultures (primary vs. passage 1) of PBECs.

Article Snippet: We recently adopted a porcine brain endothelial cell (PBEC) model first developed at Eisai Laboratories (London) by Dr. Louise Morgan and colleagues, based on a successful earlier bovine brain endothelial cell model ( ).

Techniques: Expressing