pb rtta vector Search Results


piggybac vector  (Addgene inc)
93
Addgene inc piggybac vector
Piggybac Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb+rtta+vector/PB-TRE-EGFP-EF1a-rtTA+(Plasmid+%23104454)/bio_rxiv__2025__02__27__640326-172-7-10
Average 93 stars, based on 1 article reviews
piggybac vector - by Bioz Stars, 2026-11
93/100 stars
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pb rtta vector  (Addgene inc)
93
Addgene inc pb rtta vector
A-B) Fishing cat fibroblasts were reprogrammed with PiggyBAC transposon carrying mouse C-myc, Klf4, Oct4 and Sox2 (MKOS) and mOrange (Or) as a reporter, together with an expression vector expressing transposase (TS). The fibroblasts expressed mOrange, indicating the successful integration of piggyBAC transposon. C-D) The fibroblasts were reprogrammed with piggyBAC transposon carrying inducible reprogramming cassette composed of human OCT4, KLF4, SOX2, C-Myc (OKSM) and human KLF2 and NANOG followed by Ires-Tdtomato. The transfection included transposase vector and piggyBAC expressing <t>rtTA.</t> <t>The</t> <t>transfected</t> cells were selected with puromycin and the expression of reprogramming factors were induced via Tet-on using Doxycycline. The iPSC-like colonies appeared with TdTomata expression. E-F) Reprogramming of fibroblasts using episomal vectors. The transfected cells were tested in various media including commercial HSC NutriStem and E8 medium, KOSR containing medium (KOSR) and media with 10% Fetal Bovine Serum (FBS) with LIF. The induced cells were also replated onto different matrix including mouse irradiated feeder, vitronectin and Geltrex. G-H) RNA-based reprogramming using self-replicating RNA (srRNA) expressing reprogramming factors. The testicular fibroblasts were transfected with srRNA and cultured in the presence of B18R protein before replating onto feeder cells. The iPSC-like colonies appeared in three weeks after transfection.
Pb Rtta Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb+rtta+vector/pB-rtTA+(Plasmid+%23126034)/bio_rxiv__2022__07__01__498384-80-11-14
Average 93 stars, based on 1 article reviews
pb rtta vector - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
lentiviral vector  (Addgene inc)
93
Addgene inc lentiviral vector
A-B) Fishing cat fibroblasts were reprogrammed with PiggyBAC transposon carrying mouse C-myc, Klf4, Oct4 and Sox2 (MKOS) and mOrange (Or) as a reporter, together with an expression vector expressing transposase (TS). The fibroblasts expressed mOrange, indicating the successful integration of piggyBAC transposon. C-D) The fibroblasts were reprogrammed with piggyBAC transposon carrying inducible reprogramming cassette composed of human OCT4, KLF4, SOX2, C-Myc (OKSM) and human KLF2 and NANOG followed by Ires-Tdtomato. The transfection included transposase vector and piggyBAC expressing <t>rtTA.</t> <t>The</t> <t>transfected</t> cells were selected with puromycin and the expression of reprogramming factors were induced via Tet-on using Doxycycline. The iPSC-like colonies appeared with TdTomata expression. E-F) Reprogramming of fibroblasts using episomal vectors. The transfected cells were tested in various media including commercial HSC NutriStem and E8 medium, KOSR containing medium (KOSR) and media with 10% Fetal Bovine Serum (FBS) with LIF. The induced cells were also replated onto different matrix including mouse irradiated feeder, vitronectin and Geltrex. G-H) RNA-based reprogramming using self-replicating RNA (srRNA) expressing reprogramming factors. The testicular fibroblasts were transfected with srRNA and cultured in the presence of B18R protein before replating onto feeder cells. The iPSC-like colonies appeared in three weeks after transfection.
Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb+rtta+vector/pcDNA5%2FFRT%2FTO+HIS+DNAJB2a+(Plasmid+%2319550)/pmc12396346-464-9-12
Average 93 stars, based on 1 article reviews
lentiviral vector - by Bioz Stars, 2026-11
93/100 stars
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pb rtta bsmbi vector  (Addgene inc)
94
Addgene inc pb rtta bsmbi vector
A-B) Fishing cat fibroblasts were reprogrammed with PiggyBAC transposon carrying mouse C-myc, Klf4, Oct4 and Sox2 (MKOS) and mOrange (Or) as a reporter, together with an expression vector expressing transposase (TS). The fibroblasts expressed mOrange, indicating the successful integration of piggyBAC transposon. C-D) The fibroblasts were reprogrammed with piggyBAC transposon carrying inducible reprogramming cassette composed of human OCT4, KLF4, SOX2, C-Myc (OKSM) and human KLF2 and NANOG followed by Ires-Tdtomato. The transfection included transposase vector and piggyBAC expressing <t>rtTA.</t> <t>The</t> <t>transfected</t> cells were selected with puromycin and the expression of reprogramming factors were induced via Tet-on using Doxycycline. The iPSC-like colonies appeared with TdTomata expression. E-F) Reprogramming of fibroblasts using episomal vectors. The transfected cells were tested in various media including commercial HSC NutriStem and E8 medium, KOSR containing medium (KOSR) and media with 10% Fetal Bovine Serum (FBS) with LIF. The induced cells were also replated onto different matrix including mouse irradiated feeder, vitronectin and Geltrex. G-H) RNA-based reprogramming using self-replicating RNA (srRNA) expressing reprogramming factors. The testicular fibroblasts were transfected with srRNA and cultured in the presence of B18R protein before replating onto feeder cells. The iPSC-like colonies appeared in three weeks after transfection.
Pb Rtta Bsmbi Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb+rtta+vector/PB_rtTA_BsmBI+(Plasmid+%23126028)/pmc13691081-367-17-19
Average 94 stars, based on 1 article reviews
pb rtta bsmbi vector - by Bioz Stars, 2026-11
94/100 stars
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kw110 pb ta ern  (Addgene inc)
94
Addgene inc kw110 pb ta ern

Kw110 Pb Ta Ern, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb+rtta+vector/PB-TA-ERN+(Plasmid+%2380474)/pmc08938283-48-0-5
Average 94 stars, based on 1 article reviews
kw110 pb ta ern - by Bioz Stars, 2026-11
94/100 stars
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piggybac vectors  (Addgene inc)
93
Addgene inc piggybac vectors

Piggybac Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb+rtta+vector/PB-TET-MKOS+(Plasmid+%2320959)/us09932560-520-0-21
Average 93 stars, based on 1 article reviews
piggybac vectors - by Bioz Stars, 2026-11
93/100 stars
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piggybac transposon expression vector  (Addgene inc)
92
Addgene inc piggybac transposon expression vector

Piggybac Transposon Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb+rtta+vector/PB-CA-rtTA+Adv+(Plasmid+%2320910)/pm36306542-49-4-9
Average 92 stars, based on 1 article reviews
piggybac transposon expression vector - by Bioz Stars, 2026-11
92/100 stars
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ppb-cag-rtta-ires-hygro (plasmid #102423)  (Addgene inc)
N/A
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ka0637_ppbcag-rttam2-in (plasmid #124166)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pblu-aavs1-cas9-p300-m2rtta-aavs1 (plasmid #112261)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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ppb-cag-rtta-in (plasmid #60612)  (Addgene inc)
N/A
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ppbcag-rttam2-ih (plasmid #140827)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


A-B) Fishing cat fibroblasts were reprogrammed with PiggyBAC transposon carrying mouse C-myc, Klf4, Oct4 and Sox2 (MKOS) and mOrange (Or) as a reporter, together with an expression vector expressing transposase (TS). The fibroblasts expressed mOrange, indicating the successful integration of piggyBAC transposon. C-D) The fibroblasts were reprogrammed with piggyBAC transposon carrying inducible reprogramming cassette composed of human OCT4, KLF4, SOX2, C-Myc (OKSM) and human KLF2 and NANOG followed by Ires-Tdtomato. The transfection included transposase vector and piggyBAC expressing rtTA. The transfected cells were selected with puromycin and the expression of reprogramming factors were induced via Tet-on using Doxycycline. The iPSC-like colonies appeared with TdTomata expression. E-F) Reprogramming of fibroblasts using episomal vectors. The transfected cells were tested in various media including commercial HSC NutriStem and E8 medium, KOSR containing medium (KOSR) and media with 10% Fetal Bovine Serum (FBS) with LIF. The induced cells were also replated onto different matrix including mouse irradiated feeder, vitronectin and Geltrex. G-H) RNA-based reprogramming using self-replicating RNA (srRNA) expressing reprogramming factors. The testicular fibroblasts were transfected with srRNA and cultured in the presence of B18R protein before replating onto feeder cells. The iPSC-like colonies appeared in three weeks after transfection.

Journal: bioRxiv

Article Title: Fishing Cat Cell Biobanking for Conservation

doi: 10.1101/2022.07.01.498384

Figure Lengend Snippet: A-B) Fishing cat fibroblasts were reprogrammed with PiggyBAC transposon carrying mouse C-myc, Klf4, Oct4 and Sox2 (MKOS) and mOrange (Or) as a reporter, together with an expression vector expressing transposase (TS). The fibroblasts expressed mOrange, indicating the successful integration of piggyBAC transposon. C-D) The fibroblasts were reprogrammed with piggyBAC transposon carrying inducible reprogramming cassette composed of human OCT4, KLF4, SOX2, C-Myc (OKSM) and human KLF2 and NANOG followed by Ires-Tdtomato. The transfection included transposase vector and piggyBAC expressing rtTA. The transfected cells were selected with puromycin and the expression of reprogramming factors were induced via Tet-on using Doxycycline. The iPSC-like colonies appeared with TdTomata expression. E-F) Reprogramming of fibroblasts using episomal vectors. The transfected cells were tested in various media including commercial HSC NutriStem and E8 medium, KOSR containing medium (KOSR) and media with 10% Fetal Bovine Serum (FBS) with LIF. The induced cells were also replated onto different matrix including mouse irradiated feeder, vitronectin and Geltrex. G-H) RNA-based reprogramming using self-replicating RNA (srRNA) expressing reprogramming factors. The testicular fibroblasts were transfected with srRNA and cultured in the presence of B18R protein before replating onto feeder cells. The iPSC-like colonies appeared in three weeks after transfection.

Article Snippet: We transfected pC6F alongside with transposase vector (pCy43, Sanger Institute) and PB rtTA vector (addgene 126034) using Lipofectamine 3000 (ThermoFisher) for overnight.

Techniques: Expressing, Plasmid Preparation, Transfection, Irradiation, Cell Culture

Journal: iScience

Article Title: One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors

doi: 10.1016/j.isci.2022.103987

Figure Lengend Snippet:

Article Snippet: KW110_PB_TA_ERN (Ef1a_rtTA_neo) vector , , Addgene; Plasmid #80474.

Techniques: Recombinant, Expressing, RNA Sequencing Assay, Plasmid Preparation