Journal: Cell Death & Disease

Article Title: Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progression

doi: 10.1038/s41419-026-08411-y

Figure Lengend Snippet: A A boxplot visualized the expression of HIF1A in normal tissues and primary EC tissues, using the EC transcriptome data downloaded from the TCGA database. B Kaplan-Meier survival analysis was performed to evaluate the association between HIF1A expression levels and patient prognosis. C Signal pathways of DEGs enriched by HIF1A in EC were clarified through KEGG enrichment analysis. D , E Box plots were used to analyze the expression of D lactate production-related genes or E lactate transport-related genes in EC cells with HIF1A_High or _Low expression. * P < 0.05, ** P < 0.01. F The expression of HIF1A in EC tissues and normal tissues was detected by IHC assay. Scale bar: 100 μm (200×), Scale bar: 50 μm (400×); N = 20; ** P < 0.01.

Article Snippet: HIF1A , proteintech , 20960-1-AP , 1:5000/1:200 , WB/ IHC.

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progression

doi: 10.1038/s41419-026-08411-y

Figure Lengend Snippet: A , B The effects of normoxic and hypoxic conditions on the proliferation, migration, and invasion abilities of Ishikawa and HEC-1-A cells were examined using the MTT assay ( A ) and Transwell assay ( B ). Scale bar: 100 μm; N = 3; * P < 0.05, ** P < 0.01. C The effect of hypoxia on HIF1A, LDHA, ENO2, MCT3, HK2, PGK1, and ALDOB protein levels was detected by WB. N = 3; * P < 0.05, ** P < 0.01. D The changes in lactate levels in the SN of Ishikawa and HEC-1-A cells under hypoxic or normoxic conditions were detected using a Lactate Assay Kit. N = 3; * P < 0.05. E The expression of LDHA, ENO2, MCT3, HK2, PGK1, and ALDOB in clinical EC tissues or normal tissues was detected by IHC. Scale bar: 100 μm (200×), Scale bar: 50 μm (400×); N = 20; * P < 0.05, ** P < 0.01.

Article Snippet: HIF1A , proteintech , 20960-1-AP , 1:5000/1:200 , WB/ IHC.

Techniques: Migration, MTT Assay, Transwell Assay, Lactate Assay, Expressing

Journal: Cell Death & Disease

Article Title: Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progression

doi: 10.1038/s41419-026-08411-y

Figure Lengend Snippet: A UMAP visualization & B Featureplot of 12 cell clusters identified after integration, quality control, and clustering of five EC single-cell datasets ( GSM5276933 - GSM5276937 from the GEO database). C Violin plot comparing HIF1A expression in cancer cells across samples, categorizing subgroups into HIF1A_High ( GSM5276934 , GSM5276936 , GSM5276937 ) and HIF1A_Low ( GSM5276933 , GSM5276935 ). D Subgroup-specific UMAP visualization of HIF1A_High versus HIF1A_Low samples. E Bar chart depicting cell-type proportions across HIF1A subgroups. F Bubble chart comparing HIF1A expression in different cell types between HIF1A_High and HIF1A_Low subgroups. G GO and H KEGG enrichment analysis of macrophage-related DEGs in HIF1A-stratified tumor cells in ScRNA-seq data.

Article Snippet: HIF1A , proteintech , 20960-1-AP , 1:5000/1:200 , WB/ IHC.

Techniques: Control, Single Cell, Expressing

Journal: Cell Death & Disease

Article Title: Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progression

doi: 10.1038/s41419-026-08411-y

Figure Lengend Snippet: A Flow cytometry analysis of CD163 in macrophages treated with hypoxic HEC-1-A/Ishikawa cell SN. N = 3; * P < 0.05, ** P < 0.01. B , C Proliferation ( B ) and migration/invasion ( C ) of naive HEC-1-A/Ishikawa cells exposed to M2 macrophage SN derived from normoxic/hypoxic EC-treated macrophages. Scale bar: 100 μm; N = 3; * P < 0.05, ** P < 0.01. (D-F) Macrophages were treated with SN from normoxic or hypoxic EC cells, with or without OA (10 mM) co-treatment. D H3K18la levels were analyzed by WB. E M2 polarization was assessed by CD163 expression using flow cytometry. F H3K18la expression was examined by WB under OA co-treatment conditions. N = 3; ** P < 0.01. G Flow cytometry analysis of CD163 expression in macrophages treated with SN from hypoxic Ishikawa cells transfected with siR-NC, siR-HIF1A, or siR-HIF1A with lactate supplementation (10 mM). N = 3; * P < 0.05, ** P < 0.01. H–J Ishikawa cells were transfected with either siR-NC or siR-MCT3 plasmids (siR-MCT3-1, selected for optimal knockdown efficiency), followed by treatment under normoxic or hypoxic conditions. Subsequently, the supernatants from these treated Ishikawa cells were used to treat macrophages. H MCT3 knockdown efficiency assessed in Ishikawa cells by WB assay. I Lactate levels in Ishikawa cell culture supernatant. J Macrophage polarization analyzed by CD163 expression using flow cytometry. N = 3; * P < 0.05, ** P < 0.01.

Article Snippet: HIF1A , proteintech , 20960-1-AP , 1:5000/1:200 , WB/ IHC.

Techniques: Flow Cytometry, Migration, Derivative Assay, Expressing, Transfection, Knockdown, Cell Culture