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Image Search Results
Journal: Science Advances
Article Title: Magnetically steerable bacterial microrobots moving in 3D biological matrices for stimuli-responsive cargo delivery
doi: 10.1126/sciadv.abo6163
Figure Lengend Snippet: ( A ) Schematic illustration of the bacterial biohybrid microrobots, conjugated with NLs and mNPs. NLs are loaded with DOX and ICG, and both NLs and mNPs are conjugated to bacteria via biotin-streptavidin interactions. Inset shows an SEM image of an example bacterial biohybrid carrying mNPs and NLs. Image was pseudocolored. Scale bar, 500 nm. ( B ) Flow cytometry density plots of (i) free bacteria expressing GFP, (ii) bacterial biohybrids carrying mNPs tagged with red fluorescence, and (iii) bacterial biohybrids carrying mNPs tagged with red fluorescence and NLs tagged with Cy5, showing successful conjugations quantitatively. a.u., arbitrary units. Scale bar, 2 μm. ( C ) Conceptual schematics depicting bacterial biohybrid microrobots magnetically guided through porous microenvironments toward target tissues, such as a tumor. Bacterial biohybrids can release their payload upon NIR irradiation, enabling stimuli-responsive cargo release in 3D biological matrices. N, north; S, south.
Article Snippet: Bacterial biohybrids were prepared by the streptavidin-biotin interaction between a genetically engineered strain of E. coli MG1655 with GFP and biotin expression and
Techniques: Bacteria, Flow Cytometry, Expressing, Fluorescence, Irradiation