part cremophor Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher rpmi 1640
    Rpmi 1640, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 89097 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640/product/Thermo Fisher
    Average 99 stars, based on 89097 article reviews
    Price from $9.99 to $1999.99
    rpmi 1640 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore rpmi 1640 medium
    Rpmi 1640 Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 30869 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/Millipore
    Average 99 stars, based on 30869 article reviews
    Price from $9.99 to $1999.99
    rpmi 1640 medium - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    93
    BASF cremophor
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of Pluronic P123 to Pluronic F127, and X 3 : percent of <t>Cremophor</t> EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Cremophor, supplied by BASF, used in various techniques. Bioz Stars score: 93/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cremophor/product/BASF
    Average 93 stars, based on 370 article reviews
    Price from $9.99 to $1999.99
    cremophor - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher rpmi 1640 medium
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of Pluronic P123 to Pluronic F127, and X 3 : percent of <t>Cremophor</t> EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Rpmi 1640 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 100450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/Thermo Fisher
    Average 99 stars, based on 100450 article reviews
    Price from $9.99 to $1999.99
    rpmi 1640 medium - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    90
    BASF cremophor rh40
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of Pluronic P123 to Pluronic F127, and X 3 : percent of <t>Cremophor</t> EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Cremophor Rh40, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cremophor rh40/product/BASF
    Average 90 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    cremophor rh40 - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    93
    Merck KGaA sodium chloride
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of Pluronic P123 to Pluronic F127, and X 3 : percent of <t>Cremophor</t> EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Sodium Chloride, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 713 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium chloride/product/Merck KGaA
    Average 93 stars, based on 713 article reviews
    Price from $9.99 to $1999.99
    sodium chloride - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    89
    Novartis sandimmune
    Distribution volume (DV) of [ 11 C]carvedilol in the brain at 0, 10,15, 25, and 50 mg kg −1 <t>cyclosporin</t> A. The bars represent mean DV±s.e.m. ( n =3–5). The fitted curve is mathematically described as a four-parameter Hill plot:
    Sandimmune, supplied by Novartis, used in various techniques. Bioz Stars score: 89/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sandimmune/product/Novartis
    Average 89 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    sandimmune - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc cleaved caspase 3
    Induction of apoptosis and analysis of apoptosis-related factors in IGROV-1 and IGROV-1/Pt1 and A431 cells exposed to the IC 50  PTX or vinorelbine (VIN) or ST2782 (ST) or SAHA or their combination. A) Cleavage of caspase 3 (CPP32) and PARP-1 and cytochrome C release. Western blot analysis was performed after 24 h exposure to drug concentrations used in experiments of   Fig. 2A . β-Tubulin is shown as a control of protein loading. Release of cytochrome C was examined in cytosolic and mitochondrial extracts, prepared after 24 h exposure. B) Apoptosis determined by TUNEL assay after 72 h of treatment (ST2782 10 µM, PTX 1.2 µM and SAHA 10 µM). The percentages of TUNEL-positive cells are indicated in each panel. One representative experiment of at least three is shown. The results, expressed as percentage of TUNEL-positive cells, represent the mean±SD of three independent experiments. C) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with SAHA and/or PTX to the same concentrations used for TUNEL assay. D) Analysis of cytochrome C release in cytosol and in mitochondrial extract prepared after 48 h exposure to PTX or SAHA alone or their combination in IGROV-1 cells. E) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with vinorelbine (0.047 µM) or ST2782 (10 µM) alone or in combination. F) Cleavage of CPP32 in A431 cells treated with ST2782 (ST, 3 µM) or PTX (0.0035 µM) alone or in combination. β-Tubulin is shown as a control of protein loading when whole-cell lysates are used. The size of CPP32 and of their cleavage products are indicated. An antibody against a mitochondrial marker and an antibody against actin were used as protein control to ensure a correct subcellular fractionation process.
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 38170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3/product/Cell Signaling Technology Inc
    Average 99 stars, based on 38170 article reviews
    Price from $9.99 to $1999.99
    cleaved caspase 3 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    94
    Merck KGaA potassium dihydrogen phosphate
    Induction of apoptosis and analysis of apoptosis-related factors in IGROV-1 and IGROV-1/Pt1 and A431 cells exposed to the IC 50  PTX or vinorelbine (VIN) or ST2782 (ST) or SAHA or their combination. A) Cleavage of caspase 3 (CPP32) and PARP-1 and cytochrome C release. Western blot analysis was performed after 24 h exposure to drug concentrations used in experiments of   Fig. 2A . β-Tubulin is shown as a control of protein loading. Release of cytochrome C was examined in cytosolic and mitochondrial extracts, prepared after 24 h exposure. B) Apoptosis determined by TUNEL assay after 72 h of treatment (ST2782 10 µM, PTX 1.2 µM and SAHA 10 µM). The percentages of TUNEL-positive cells are indicated in each panel. One representative experiment of at least three is shown. The results, expressed as percentage of TUNEL-positive cells, represent the mean±SD of three independent experiments. C) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with SAHA and/or PTX to the same concentrations used for TUNEL assay. D) Analysis of cytochrome C release in cytosol and in mitochondrial extract prepared after 48 h exposure to PTX or SAHA alone or their combination in IGROV-1 cells. E) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with vinorelbine (0.047 µM) or ST2782 (10 µM) alone or in combination. F) Cleavage of CPP32 in A431 cells treated with ST2782 (ST, 3 µM) or PTX (0.0035 µM) alone or in combination. β-Tubulin is shown as a control of protein loading when whole-cell lysates are used. The size of CPP32 and of their cleavage products are indicated. An antibody against a mitochondrial marker and an antibody against actin were used as protein control to ensure a correct subcellular fractionation process.
    Potassium Dihydrogen Phosphate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/potassium dihydrogen phosphate/product/Merck KGaA
    Average 94 stars, based on 408 article reviews
    Price from $9.99 to $1999.99
    potassium dihydrogen phosphate - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    92
    Merck KGaA disodium hydrogen phosphate
    Induction of apoptosis and analysis of apoptosis-related factors in IGROV-1 and IGROV-1/Pt1 and A431 cells exposed to the IC 50  PTX or vinorelbine (VIN) or ST2782 (ST) or SAHA or their combination. A) Cleavage of caspase 3 (CPP32) and PARP-1 and cytochrome C release. Western blot analysis was performed after 24 h exposure to drug concentrations used in experiments of   Fig. 2A . β-Tubulin is shown as a control of protein loading. Release of cytochrome C was examined in cytosolic and mitochondrial extracts, prepared after 24 h exposure. B) Apoptosis determined by TUNEL assay after 72 h of treatment (ST2782 10 µM, PTX 1.2 µM and SAHA 10 µM). The percentages of TUNEL-positive cells are indicated in each panel. One representative experiment of at least three is shown. The results, expressed as percentage of TUNEL-positive cells, represent the mean±SD of three independent experiments. C) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with SAHA and/or PTX to the same concentrations used for TUNEL assay. D) Analysis of cytochrome C release in cytosol and in mitochondrial extract prepared after 48 h exposure to PTX or SAHA alone or their combination in IGROV-1 cells. E) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with vinorelbine (0.047 µM) or ST2782 (10 µM) alone or in combination. F) Cleavage of CPP32 in A431 cells treated with ST2782 (ST, 3 µM) or PTX (0.0035 µM) alone or in combination. β-Tubulin is shown as a control of protein loading when whole-cell lysates are used. The size of CPP32 and of their cleavage products are indicated. An antibody against a mitochondrial marker and an antibody against actin were used as protein control to ensure a correct subcellular fractionation process.
    Disodium Hydrogen Phosphate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disodium hydrogen phosphate/product/Merck KGaA
    Average 92 stars, based on 169 article reviews
    Price from $9.99 to $1999.99
    disodium hydrogen phosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    88
    ABITEC capmul mcm nf
    Induction of apoptosis and analysis of apoptosis-related factors in IGROV-1 and IGROV-1/Pt1 and A431 cells exposed to the IC 50  PTX or vinorelbine (VIN) or ST2782 (ST) or SAHA or their combination. A) Cleavage of caspase 3 (CPP32) and PARP-1 and cytochrome C release. Western blot analysis was performed after 24 h exposure to drug concentrations used in experiments of   Fig. 2A . β-Tubulin is shown as a control of protein loading. Release of cytochrome C was examined in cytosolic and mitochondrial extracts, prepared after 24 h exposure. B) Apoptosis determined by TUNEL assay after 72 h of treatment (ST2782 10 µM, PTX 1.2 µM and SAHA 10 µM). The percentages of TUNEL-positive cells are indicated in each panel. One representative experiment of at least three is shown. The results, expressed as percentage of TUNEL-positive cells, represent the mean±SD of three independent experiments. C) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with SAHA and/or PTX to the same concentrations used for TUNEL assay. D) Analysis of cytochrome C release in cytosol and in mitochondrial extract prepared after 48 h exposure to PTX or SAHA alone or their combination in IGROV-1 cells. E) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with vinorelbine (0.047 µM) or ST2782 (10 µM) alone or in combination. F) Cleavage of CPP32 in A431 cells treated with ST2782 (ST, 3 µM) or PTX (0.0035 µM) alone or in combination. β-Tubulin is shown as a control of protein loading when whole-cell lysates are used. The size of CPP32 and of their cleavage products are indicated. An antibody against a mitochondrial marker and an antibody against actin were used as protein control to ensure a correct subcellular fractionation process.
    Capmul Mcm Nf, supplied by ABITEC, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/capmul mcm nf/product/ABITEC
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    capmul mcm nf - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    88
    BASF kollidon va64
    Gibbs free energy of mixing (ΔGmix) diagrams of atovaquone in polymer carriers with the SEC from formulation 4 present. ( a ) Atovaquone and PVP K30. ( b ) Atovaquone and <t>Kollidon</t> <t>VA64.</t> ( c ) Atovaquone and Affinisol 15LV.
    Kollidon Va64, supplied by BASF, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kollidon va64/product/BASF
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    kollidon va64 - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    90
    BASF pluronic p123
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of <t>Pluronic</t> <t>P123</t> to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Pluronic P123, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pluronic p123/product/BASF
    Average 90 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    pluronic p123 - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    92
    ABITEC captex 300 nf
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of <t>Pluronic</t> <t>P123</t> to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Captex 300 Nf, supplied by ABITEC, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/captex 300 nf/product/ABITEC
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    captex 300 nf - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Millipore sodium citrate
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of <t>Pluronic</t> <t>P123</t> to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Sodium Citrate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium citrate/product/Millipore
    Average 99 stars, based on 5701 article reviews
    Price from $9.99 to $1999.99
    sodium citrate - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore ethanol
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of <t>Pluronic</t> <t>P123</t> to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Ethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethanol/product/Millipore
    Average 99 stars, based on 8197 article reviews
    Price from $9.99 to $1999.99
    ethanol - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore fibronectin
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of <t>Pluronic</t> <t>P123</t> to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Fibronectin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 20291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin/product/Millipore
    Average 99 stars, based on 20291 article reviews
    Price from $9.99 to $1999.99
    fibronectin - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore polyoxyethylene 20 sorbitan monooleate
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of <t>Pluronic</t> <t>P123</t> to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Polyoxyethylene 20 Sorbitan Monooleate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyoxyethylene 20 sorbitan monooleate/product/Millipore
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    polyoxyethylene 20 sorbitan monooleate - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Sanofi polysorbate 80
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of <t>Pluronic</t> <t>P123</t> to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Polysorbate 80, supplied by Sanofi, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polysorbate 80/product/Sanofi
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    polysorbate 80 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    90
    BASF kollidon 30
    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of <t>Pluronic</t> <t>P123</t> to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).
    Kollidon 30, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kollidon 30/product/BASF
    Average 90 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    kollidon 30 - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    92
    Actavis paclitaxel
    Effect of <t>paclitaxel</t> administration on heat hypersensitivity. Graph shows the mean ± SEM of the hind paw withdrawal latency to a radiant heat stimulus. BL = baseline before paclitaxel/vehicle administration. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6 ( n =6 per group).
    Paclitaxel, supplied by Actavis, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paclitaxel/product/Actavis
    Average 92 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    paclitaxel - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Millipore paclitaxel
    The Bclw BH4 domain is sufficient to prevent <t>paclitaxel-induced</t> axon degeneration (A) Schematic of stabilized alpha-helix of Bcl2 domain (SAHB) peptides modeled after the BH4 domains of Bclw (PDB ID 4CIM, aa 12-31), Bcl2 (PDB ID 2XA0, aa 13-32), and Bclx L (PDB ID 4QVE, aa 6-26). (B) Percent release of ANTS/DPX encapsulated liposomes in the presence of Bax, Bim SAHB A2 , and/or Bclw BH4 SAHB A as indicated; n=3; data represent mean ± SD. (C) Axons from compartmented cultures after protein transfection with FITC-BH4 SAHB peptides of Bclw, Bcl2, or Bclx L showing FITC signal (left) and Tuj1 signal (right); scale bar = 20 µm. (D) Degeneration index of axons treated with paclitaxel or DMSO after protein transfection of FITC-BH4 SAHB-Bclw, Bcl2, or Bclx L into axons; *p
    Paclitaxel, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paclitaxel/product/Millipore
    Average 99 stars, based on 5901 article reviews
    Price from $9.99 to $1999.99
    paclitaxel - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    BASF pluronic f 127
    The Bclw BH4 domain is sufficient to prevent <t>paclitaxel-induced</t> axon degeneration (A) Schematic of stabilized alpha-helix of Bcl2 domain (SAHB) peptides modeled after the BH4 domains of Bclw (PDB ID 4CIM, aa 12-31), Bcl2 (PDB ID 2XA0, aa 13-32), and Bclx L (PDB ID 4QVE, aa 6-26). (B) Percent release of ANTS/DPX encapsulated liposomes in the presence of Bax, Bim SAHB A2 , and/or Bclw BH4 SAHB A as indicated; n=3; data represent mean ± SD. (C) Axons from compartmented cultures after protein transfection with FITC-BH4 SAHB peptides of Bclw, Bcl2, or Bclx L showing FITC signal (left) and Tuj1 signal (right); scale bar = 20 µm. (D) Degeneration index of axons treated with paclitaxel or DMSO after protein transfection of FITC-BH4 SAHB-Bclw, Bcl2, or Bclx L into axons; *p
    Pluronic F 127, supplied by BASF, used in various techniques. Bioz Stars score: 92/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pluronic f 127/product/BASF
    Average 92 stars, based on 248 article reviews
    Price from $9.99 to $1999.99
    pluronic f 127 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    94
    Pfizer Inc medetomidine
    The Bclw BH4 domain is sufficient to prevent <t>paclitaxel-induced</t> axon degeneration (A) Schematic of stabilized alpha-helix of Bcl2 domain (SAHB) peptides modeled after the BH4 domains of Bclw (PDB ID 4CIM, aa 12-31), Bcl2 (PDB ID 2XA0, aa 13-32), and Bclx L (PDB ID 4QVE, aa 6-26). (B) Percent release of ANTS/DPX encapsulated liposomes in the presence of Bax, Bim SAHB A2 , and/or Bclw BH4 SAHB A as indicated; n=3; data represent mean ± SD. (C) Axons from compartmented cultures after protein transfection with FITC-BH4 SAHB peptides of Bclw, Bcl2, or Bclx L showing FITC signal (left) and Tuj1 signal (right); scale bar = 20 µm. (D) Degeneration index of axons treated with paclitaxel or DMSO after protein transfection of FITC-BH4 SAHB-Bclw, Bcl2, or Bclx L into axons; *p
    Medetomidine, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 94/100, based on 1197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/medetomidine/product/Pfizer Inc
    Average 94 stars, based on 1197 article reviews
    Price from $9.99 to $1999.99
    medetomidine - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc caspase 3
    Activation of apoptotic pathway by gefitinib and chloroquine in PC-9/wt and PC-9/gef ells. PC-9/wt, PC-9/gefB4 and PC-9/gefE3 cells were treated with gefitinib (100 nM) and chloroquine (CQ, 10 μM) for 24 h. Total protein of treated cells was harvested. Procaspase 3, active caspase 3, PARP cleavage levels was analyzed with Western blot assay. Each lane contained 30 μg protein for all experiments. Results were repeated in independent experiments.
    Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 27981 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3/product/Cell Signaling Technology Inc
    Average 94 stars, based on 27981 article reviews
    Price from $9.99 to $1999.99
    caspase 3 - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of Pluronic P123 to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).

    Journal: Drug Delivery

    Article Title: Formulation and in vivo assessment of terconazole-loaded polymeric mixed micelles enriched with Cremophor EL as dual functioning mediator for augmenting physical stability and skin delivery

    doi: 10.1080/10717544.2018.1436098

    Figure Lengend Snippet: 3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of Pluronic P123 to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).

    Article Snippet: Pluronic F127, Pluronic P123, and Cremophor EL were purchased from BASF Co. (Florham Park, NJ).

    Techniques:

    (a) Cumulative amount of TCZ permeated per unit area across skin via optimal PMMs formulation (F7) compared to PMMs lacking Cremophor EL and TCZ suspension. (b) Cumulative amount of TCZ deposited per unit area in the skin via optimal PMMs formulation (F7) compared to TCZ suspension.

    Journal: Drug Delivery

    Article Title: Formulation and in vivo assessment of terconazole-loaded polymeric mixed micelles enriched with Cremophor EL as dual functioning mediator for augmenting physical stability and skin delivery

    doi: 10.1080/10717544.2018.1436098

    Figure Lengend Snippet: (a) Cumulative amount of TCZ permeated per unit area across skin via optimal PMMs formulation (F7) compared to PMMs lacking Cremophor EL and TCZ suspension. (b) Cumulative amount of TCZ deposited per unit area in the skin via optimal PMMs formulation (F7) compared to TCZ suspension.

    Article Snippet: Pluronic F127, Pluronic P123, and Cremophor EL were purchased from BASF Co. (Florham Park, NJ).

    Techniques:

    Distribution volume (DV) of [ 11 C]carvedilol in the brain at 0, 10,15, 25, and 50 mg kg −1 cyclosporin A. The bars represent mean DV±s.e.m. ( n =3–5). The fitted curve is mathematically described as a four-parameter Hill plot:

    Journal: British Journal of Pharmacology

    Article Title: New positron emission tomography tracer [11C]carvedilol reveals P-glycoprotein modulation kinetics

    doi: 10.1038/sj.bjp.0706283

    Figure Lengend Snippet: Distribution volume (DV) of [ 11 C]carvedilol in the brain at 0, 10,15, 25, and 50 mg kg −1 cyclosporin A. The bars represent mean DV±s.e.m. ( n =3–5). The fitted curve is mathematically described as a four-parameter Hill plot:

    Article Snippet: Medetomidine (Domitor®, 1 mg medetomidine HCl ml−1 ) from Pfizer Animal Health B.V. (Capelle a/d IJssel, the Netherlands), CsA (50 mg ml−1 ) in cremophore EL (650 mg ml−1 ) (Sandimmune®) from Novartis (Basel, Switzerland), and MK 571 from Alexis Corp. (Lausen, Switzerland).

    Techniques:

    Plasma clearance of [ 11 C]carvedilol in the presence of several dosages cyclosporin A. Each point represents the mean±s.e.m.

    Journal: British Journal of Pharmacology

    Article Title: New positron emission tomography tracer [11C]carvedilol reveals P-glycoprotein modulation kinetics

    doi: 10.1038/sj.bjp.0706283

    Figure Lengend Snippet: Plasma clearance of [ 11 C]carvedilol in the presence of several dosages cyclosporin A. Each point represents the mean±s.e.m.

    Article Snippet: Medetomidine (Domitor®, 1 mg medetomidine HCl ml−1 ) from Pfizer Animal Health B.V. (Capelle a/d IJssel, the Netherlands), CsA (50 mg ml−1 ) in cremophore EL (650 mg ml−1 ) (Sandimmune®) from Novartis (Basel, Switzerland), and MK 571 from Alexis Corp. (Lausen, Switzerland).

    Techniques:

    Time–activity curves of [ 11 C]carvedilol in the brain with several dosages cyclosporin A, measured by PET. Each point represents mean±s.e.m.

    Journal: British Journal of Pharmacology

    Article Title: New positron emission tomography tracer [11C]carvedilol reveals P-glycoprotein modulation kinetics

    doi: 10.1038/sj.bjp.0706283

    Figure Lengend Snippet: Time–activity curves of [ 11 C]carvedilol in the brain with several dosages cyclosporin A, measured by PET. Each point represents mean±s.e.m.

    Article Snippet: Medetomidine (Domitor®, 1 mg medetomidine HCl ml−1 ) from Pfizer Animal Health B.V. (Capelle a/d IJssel, the Netherlands), CsA (50 mg ml−1 ) in cremophore EL (650 mg ml−1 ) (Sandimmune®) from Novartis (Basel, Switzerland), and MK 571 from Alexis Corp. (Lausen, Switzerland).

    Techniques: Activity Assay, Positron Emission Tomography

    Induction of apoptosis and analysis of apoptosis-related factors in IGROV-1 and IGROV-1/Pt1 and A431 cells exposed to the IC 50  PTX or vinorelbine (VIN) or ST2782 (ST) or SAHA or their combination. A) Cleavage of caspase 3 (CPP32) and PARP-1 and cytochrome C release. Western blot analysis was performed after 24 h exposure to drug concentrations used in experiments of   Fig. 2A . β-Tubulin is shown as a control of protein loading. Release of cytochrome C was examined in cytosolic and mitochondrial extracts, prepared after 24 h exposure. B) Apoptosis determined by TUNEL assay after 72 h of treatment (ST2782 10 µM, PTX 1.2 µM and SAHA 10 µM). The percentages of TUNEL-positive cells are indicated in each panel. One representative experiment of at least three is shown. The results, expressed as percentage of TUNEL-positive cells, represent the mean±SD of three independent experiments. C) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with SAHA and/or PTX to the same concentrations used for TUNEL assay. D) Analysis of cytochrome C release in cytosol and in mitochondrial extract prepared after 48 h exposure to PTX or SAHA alone or their combination in IGROV-1 cells. E) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with vinorelbine (0.047 µM) or ST2782 (10 µM) alone or in combination. F) Cleavage of CPP32 in A431 cells treated with ST2782 (ST, 3 µM) or PTX (0.0035 µM) alone or in combination. β-Tubulin is shown as a control of protein loading when whole-cell lysates are used. The size of CPP32 and of their cleavage products are indicated. An antibody against a mitochondrial marker and an antibody against actin were used as protein control to ensure a correct subcellular fractionation process.

    Journal: PLoS ONE

    Article Title: Synergistic Antitumor Effects of Novel HDAC Inhibitors and Paclitaxel In Vitro and In Vivo

    doi: 10.1371/journal.pone.0029085

    Figure Lengend Snippet: Induction of apoptosis and analysis of apoptosis-related factors in IGROV-1 and IGROV-1/Pt1 and A431 cells exposed to the IC 50 PTX or vinorelbine (VIN) or ST2782 (ST) or SAHA or their combination. A) Cleavage of caspase 3 (CPP32) and PARP-1 and cytochrome C release. Western blot analysis was performed after 24 h exposure to drug concentrations used in experiments of Fig. 2A . β-Tubulin is shown as a control of protein loading. Release of cytochrome C was examined in cytosolic and mitochondrial extracts, prepared after 24 h exposure. B) Apoptosis determined by TUNEL assay after 72 h of treatment (ST2782 10 µM, PTX 1.2 µM and SAHA 10 µM). The percentages of TUNEL-positive cells are indicated in each panel. One representative experiment of at least three is shown. The results, expressed as percentage of TUNEL-positive cells, represent the mean±SD of three independent experiments. C) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with SAHA and/or PTX to the same concentrations used for TUNEL assay. D) Analysis of cytochrome C release in cytosol and in mitochondrial extract prepared after 48 h exposure to PTX or SAHA alone or their combination in IGROV-1 cells. E) Cleavage of CPP32 and PARP-1 in IGROV-1 cells treated with vinorelbine (0.047 µM) or ST2782 (10 µM) alone or in combination. F) Cleavage of CPP32 in A431 cells treated with ST2782 (ST, 3 µM) or PTX (0.0035 µM) alone or in combination. β-Tubulin is shown as a control of protein loading when whole-cell lysates are used. The size of CPP32 and of their cleavage products are indicated. An antibody against a mitochondrial marker and an antibody against actin were used as protein control to ensure a correct subcellular fractionation process.

    Article Snippet: After dilution, the drug was kept in ice and administered i.v. We used primary antibodies against p53 (Dako, Glostrup, Denmark); p21WAF1/Cip1 (Neomarker, Union City, CA); caspase-3 (CPP32), cleaved caspase-3 (Asp175 ), acetylated p53 (lys382 ) (Cell Signalling Technology, Beverly, MA); PARP-1 (Oncogene Science, Uniondale, NY); cytochrome C (BD Pharmingen, Becton Dickinson, Franklin Lakes, NJ); actin, acetylated tubulin and β-tubulin (Sigma, St. Louis, MO); mitotic protein monoclonal 2 (MPM-2) (Upstate Biotechnology, Lake Placid, NY); Raf-1 (sc-7267), cyclin-B1 (sc-245), cdc-25C (sc-327) (Santa Cruz Biotechnology, Santa Cruz, CA); mitochondrial marker (Abcam, Cambridge, UK); topoisomerase II (Transduction Laboratories, Lexington).

    Techniques: Western Blot, TUNEL Assay, Marker, Fractionation

    Gibbs free energy of mixing (ΔGmix) diagrams of atovaquone in polymer carriers with the SEC from formulation 4 present. ( a ) Atovaquone and PVP K30. ( b ) Atovaquone and Kollidon VA64. ( c ) Atovaquone and Affinisol 15LV.

    Journal: Pharmaceutics

    Article Title: A Repurposed Drug for Brain Cancer: Enhanced Atovaquone Amorphous Solid Dispersion by Combining a Spontaneously Emulsifying Component with a Polymer Carrier

    doi: 10.3390/pharmaceutics10020060

    Figure Lengend Snippet: Gibbs free energy of mixing (ΔGmix) diagrams of atovaquone in polymer carriers with the SEC from formulation 4 present. ( a ) Atovaquone and PVP K30. ( b ) Atovaquone and Kollidon VA64. ( c ) Atovaquone and Affinisol 15LV.

    Article Snippet: Kollidon VA64, Kollidon 30, and Cremophor RH 40 were kindly donated from BASF Corp. (Florham Park, NJ, USA).

    Techniques: Size-exclusion Chromatography

    3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of Pluronic P123 to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).

    Journal: Drug Delivery

    Article Title: Formulation and in vivo assessment of terconazole-loaded polymeric mixed micelles enriched with Cremophor EL as dual functioning mediator for augmenting physical stability and skin delivery

    doi: 10.1080/10717544.2018.1436098

    Figure Lengend Snippet: 3-D plot model graphs demonstrating the effect of factors; X 1 : weight ratio of total Pluronics to drug, X 2 : weight ratio of Pluronic P123 to Pluronic F127, and X 3 : percent of Cremophor EL in aqueous medium on micellar incorporation efficiency percent (MIE%) (a and b) and mean micellar size (c and d).

    Article Snippet: Pluronic F127, Pluronic P123, and Cremophor EL were purchased from BASF Co. (Florham Park, NJ).

    Techniques:

    Effect of paclitaxel administration on heat hypersensitivity. Graph shows the mean ± SEM of the hind paw withdrawal latency to a radiant heat stimulus. BL = baseline before paclitaxel/vehicle administration. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6 ( n =6 per group).

    Journal: Pain Research & Management

    Article Title: Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy

    doi: 10.1155/2018/8217613

    Figure Lengend Snippet: Effect of paclitaxel administration on heat hypersensitivity. Graph shows the mean ± SEM of the hind paw withdrawal latency to a radiant heat stimulus. BL = baseline before paclitaxel/vehicle administration. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6 ( n =6 per group).

    Article Snippet: When using paclitaxel from Actavis, the vehicle stock was supplemented with 2 mg/mL sodium citrate (Sigma, UK).

    Techniques:

    Effect of paclitaxel administration on motor coordination. Graph shows the mean ± SEM of the latency to fall from an accelerating RotaRod apparatus. BL = baseline before paclitaxel/vehicle administration. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6 ( n =6 per group).

    Journal: Pain Research & Management

    Article Title: Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy

    doi: 10.1155/2018/8217613

    Figure Lengend Snippet: Effect of paclitaxel administration on motor coordination. Graph shows the mean ± SEM of the latency to fall from an accelerating RotaRod apparatus. BL = baseline before paclitaxel/vehicle administration. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6 ( n =6 per group).

    Article Snippet: When using paclitaxel from Actavis, the vehicle stock was supplemented with 2 mg/mL sodium citrate (Sigma, UK).

    Techniques:

    Time course of paclitaxel-induced mechanical hypersensitivity. Graphs show the mean ± SEM of the number of withdrawal responses to (a) von Frey 4 g, (b) von Frey 8 g, and (c) von Frey 15 g, respectively. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6. BL = baseline before paclitaxel/vehicle administration. Res = resolution of mechanical hypersensitivity, which occurred between days 174 and 219. ∗ p

    Journal: Pain Research & Management

    Article Title: Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy

    doi: 10.1155/2018/8217613

    Figure Lengend Snippet: Time course of paclitaxel-induced mechanical hypersensitivity. Graphs show the mean ± SEM of the number of withdrawal responses to (a) von Frey 4 g, (b) von Frey 8 g, and (c) von Frey 15 g, respectively. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6. BL = baseline before paclitaxel/vehicle administration. Res = resolution of mechanical hypersensitivity, which occurred between days 174 and 219. ∗ p

    Article Snippet: When using paclitaxel from Actavis, the vehicle stock was supplemented with 2 mg/mL sodium citrate (Sigma, UK).

    Techniques:

    Effect of paclitaxel administration on overnight spontaneous wheel-running behaviour. Graphs show the mean ± SEM of the (a) time spent on the wheel, (b) total distance travelled, (c) maximal acceleration generated, and (d) number of times the wheel was accessed by individual rats overnight (7.10 pm–6.30 am) before paclitaxel/vehicle administration (BL) and then on day 7, day 28, and day 35 following the initial injection of 2 mg/kg paclitaxel or vehicle. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6. ∗ p

    Journal: Pain Research & Management

    Article Title: Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy

    doi: 10.1155/2018/8217613

    Figure Lengend Snippet: Effect of paclitaxel administration on overnight spontaneous wheel-running behaviour. Graphs show the mean ± SEM of the (a) time spent on the wheel, (b) total distance travelled, (c) maximal acceleration generated, and (d) number of times the wheel was accessed by individual rats overnight (7.10 pm–6.30 am) before paclitaxel/vehicle administration (BL) and then on day 7, day 28, and day 35 following the initial injection of 2 mg/kg paclitaxel or vehicle. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6. ∗ p

    Article Snippet: When using paclitaxel from Actavis, the vehicle stock was supplemented with 2 mg/mL sodium citrate (Sigma, UK).

    Techniques: Generated, Injection

    Time course of paclitaxel-induced cold hypersensitivity. Graph shows the median ± interquartile range of the hind paw response to acetone application catergorised by the cold score. BL = baseline before paclitaxel/vehicle administration. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6. ∗ p

    Journal: Pain Research & Management

    Article Title: Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy

    doi: 10.1155/2018/8217613

    Figure Lengend Snippet: Time course of paclitaxel-induced cold hypersensitivity. Graph shows the median ± interquartile range of the hind paw response to acetone application catergorised by the cold score. BL = baseline before paclitaxel/vehicle administration. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6. ∗ p

    Article Snippet: When using paclitaxel from Actavis, the vehicle stock was supplemented with 2 mg/mL sodium citrate (Sigma, UK).

    Techniques:

    Time course of weight gain in rats following paclitaxel or vehicle administration. Graph shows mean ± SEM of rats' weight (g) from before paclitaxel/vehicle administration (BL) up to day 174. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6 ( n =18 per group).

    Journal: Pain Research & Management

    Article Title: Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy

    doi: 10.1155/2018/8217613

    Figure Lengend Snippet: Time course of weight gain in rats following paclitaxel or vehicle administration. Graph shows mean ± SEM of rats' weight (g) from before paclitaxel/vehicle administration (BL) up to day 174. Arrows indicate four injections of 2 mg/kg paclitaxel or equivalent volume of vehicle on days 0, 2, 4, and 6 ( n =18 per group).

    Article Snippet: When using paclitaxel from Actavis, the vehicle stock was supplemented with 2 mg/mL sodium citrate (Sigma, UK).

    Techniques:

    Effect of paclitaxel administration on spontaneous burrowing behaviour. Graphs show the mean ± SEM of (a) gravel displaced from burrowing tubes and (b) latency to start burrowing by individual rats before paclitaxel/vehicle administration (BL) and then on day 7 and day 28 following the initial injection of 2 mg/kg paclitaxel or vehicle. ∗ p

    Journal: Pain Research & Management

    Article Title: Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy

    doi: 10.1155/2018/8217613

    Figure Lengend Snippet: Effect of paclitaxel administration on spontaneous burrowing behaviour. Graphs show the mean ± SEM of (a) gravel displaced from burrowing tubes and (b) latency to start burrowing by individual rats before paclitaxel/vehicle administration (BL) and then on day 7 and day 28 following the initial injection of 2 mg/kg paclitaxel or vehicle. ∗ p

    Article Snippet: When using paclitaxel from Actavis, the vehicle stock was supplemented with 2 mg/mL sodium citrate (Sigma, UK).

    Techniques: Injection

    The Bclw BH4 domain is sufficient to prevent paclitaxel-induced axon degeneration (A) Schematic of stabilized alpha-helix of Bcl2 domain (SAHB) peptides modeled after the BH4 domains of Bclw (PDB ID 4CIM, aa 12-31), Bcl2 (PDB ID 2XA0, aa 13-32), and Bclx L (PDB ID 4QVE, aa 6-26). (B) Percent release of ANTS/DPX encapsulated liposomes in the presence of Bax, Bim SAHB A2 , and/or Bclw BH4 SAHB A as indicated; n=3; data represent mean ± SD. (C) Axons from compartmented cultures after protein transfection with FITC-BH4 SAHB peptides of Bclw, Bcl2, or Bclx L showing FITC signal (left) and Tuj1 signal (right); scale bar = 20 µm. (D) Degeneration index of axons treated with paclitaxel or DMSO after protein transfection of FITC-BH4 SAHB-Bclw, Bcl2, or Bclx L into axons; *p

    Journal: Neuron

    Article Title: Paclitaxel reduces axonal Bclw to initiate IP3R1-dependent axon degeneration

    doi: 10.1016/j.neuron.2017.09.034

    Figure Lengend Snippet: The Bclw BH4 domain is sufficient to prevent paclitaxel-induced axon degeneration (A) Schematic of stabilized alpha-helix of Bcl2 domain (SAHB) peptides modeled after the BH4 domains of Bclw (PDB ID 4CIM, aa 12-31), Bcl2 (PDB ID 2XA0, aa 13-32), and Bclx L (PDB ID 4QVE, aa 6-26). (B) Percent release of ANTS/DPX encapsulated liposomes in the presence of Bax, Bim SAHB A2 , and/or Bclw BH4 SAHB A as indicated; n=3; data represent mean ± SD. (C) Axons from compartmented cultures after protein transfection with FITC-BH4 SAHB peptides of Bclw, Bcl2, or Bclx L showing FITC signal (left) and Tuj1 signal (right); scale bar = 20 µm. (D) Degeneration index of axons treated with paclitaxel or DMSO after protein transfection of FITC-BH4 SAHB-Bclw, Bcl2, or Bclx L into axons; *p

    Article Snippet: Paclitaxel was prepared as 1 part 6 mg/mL paclitaxel stock solution diluted in vehicle (1:1 v/v Cremophor EL [EMD Millipore] and dehydrated ethanol) and 2 parts sterile saline and injected at 10 µL/g.

    Techniques: Transfection

    Loss of Bclw exacerbates paclitaxel-induced neuropathy in vivo . (A) Thermal pain threshold measured as time to lick or withdraw hindpaw on a 50°C hot-plate. (B) Mechanical pain threshold determined from response to von Frey filaments. (C-F) Quantification and representative images of Tuj1 positive sensory fibers (green, arrowheads) entering the epidermis per 225 µm epidermal length in thick (C-D) and thin (E-F) skin, DAPI counterstain (blue); scale bar = 25 µm; *p

    Journal: Neuron

    Article Title: Paclitaxel reduces axonal Bclw to initiate IP3R1-dependent axon degeneration

    doi: 10.1016/j.neuron.2017.09.034

    Figure Lengend Snippet: Loss of Bclw exacerbates paclitaxel-induced neuropathy in vivo . (A) Thermal pain threshold measured as time to lick or withdraw hindpaw on a 50°C hot-plate. (B) Mechanical pain threshold determined from response to von Frey filaments. (C-F) Quantification and representative images of Tuj1 positive sensory fibers (green, arrowheads) entering the epidermis per 225 µm epidermal length in thick (C-D) and thin (E-F) skin, DAPI counterstain (blue); scale bar = 25 µm; *p

    Article Snippet: Paclitaxel was prepared as 1 part 6 mg/mL paclitaxel stock solution diluted in vehicle (1:1 v/v Cremophor EL [EMD Millipore] and dehydrated ethanol) and 2 parts sterile saline and injected at 10 µL/g.

    Techniques: In Vivo

    Paclitaxel reduces axonal Bclw mRNA and protein levels bclw (A), bcl2 (D) and bclx L (G) mRNA analyzed by qRT-PCR from cell body or axon lysate of compartmented cultures after 24 hours of paclitaxel or vehicle treatment to axons. Data normalized to gapdh ; *p

    Journal: Neuron

    Article Title: Paclitaxel reduces axonal Bclw to initiate IP3R1-dependent axon degeneration

    doi: 10.1016/j.neuron.2017.09.034

    Figure Lengend Snippet: Paclitaxel reduces axonal Bclw mRNA and protein levels bclw (A), bcl2 (D) and bclx L (G) mRNA analyzed by qRT-PCR from cell body or axon lysate of compartmented cultures after 24 hours of paclitaxel or vehicle treatment to axons. Data normalized to gapdh ; *p

    Article Snippet: Paclitaxel was prepared as 1 part 6 mg/mL paclitaxel stock solution diluted in vehicle (1:1 v/v Cremophor EL [EMD Millipore] and dehydrated ethanol) and 2 parts sterile saline and injected at 10 µL/g.

    Techniques: Quantitative RT-PCR

    Paclitaxel reduces translation of Bclw via reduction of SFPQ (A) Western blot and (B) quantification of SFPQ protein from cell body or distal axon lysate of compartmented cultures after 24 hours of paclitaxel treatment to axons; data normalized to actin; *p

    Journal: Neuron

    Article Title: Paclitaxel reduces axonal Bclw to initiate IP3R1-dependent axon degeneration

    doi: 10.1016/j.neuron.2017.09.034

    Figure Lengend Snippet: Paclitaxel reduces translation of Bclw via reduction of SFPQ (A) Western blot and (B) quantification of SFPQ protein from cell body or distal axon lysate of compartmented cultures after 24 hours of paclitaxel treatment to axons; data normalized to actin; *p

    Article Snippet: Paclitaxel was prepared as 1 part 6 mg/mL paclitaxel stock solution diluted in vehicle (1:1 v/v Cremophor EL [EMD Millipore] and dehydrated ethanol) and 2 parts sterile saline and injected at 10 µL/g.

    Techniques: Western Blot

    Axonal Bclw prevents paclitaxel-induced degeneration (A) Binarized images of Tuj1 immunostaining of axons treated with paclitaxel or vehicle after protein transfection with Bclw, Bcl2, Bclx L . (B) Degeneration index of (A) and untransfected (Untrans) control; *p

    Journal: Neuron

    Article Title: Paclitaxel reduces axonal Bclw to initiate IP3R1-dependent axon degeneration

    doi: 10.1016/j.neuron.2017.09.034

    Figure Lengend Snippet: Axonal Bclw prevents paclitaxel-induced degeneration (A) Binarized images of Tuj1 immunostaining of axons treated with paclitaxel or vehicle after protein transfection with Bclw, Bcl2, Bclx L . (B) Degeneration index of (A) and untransfected (Untrans) control; *p

    Article Snippet: Paclitaxel was prepared as 1 part 6 mg/mL paclitaxel stock solution diluted in vehicle (1:1 v/v Cremophor EL [EMD Millipore] and dehydrated ethanol) and 2 parts sterile saline and injected at 10 µL/g.

    Techniques: Immunostaining, Transfection

    Paclitaxel acts locally to induce axon degeneration without cell body apoptosis (A) Tuj1 immunostaining and corresponding binarized images of axons of E15 DRG neurons grown in compartmented cultures treated with 30 nM paclitaxel or DMSO vehicle control for 24 hours to cell body (left) or distal axon (right) compartments; scale bar = 40 µm. Red boxes outline regions shown at higher magnification in the center panels. Features characteristic of degenerating axons are seen: red arrowheads indicate a “beads on a string” appearance and the red lines indicate interruptions in Tuj1 continuity. (B) Quantification of axonal degeneration: ratio of area of fragmented axons to total axon area (degeneration index); *p

    Journal: Neuron

    Article Title: Paclitaxel reduces axonal Bclw to initiate IP3R1-dependent axon degeneration

    doi: 10.1016/j.neuron.2017.09.034

    Figure Lengend Snippet: Paclitaxel acts locally to induce axon degeneration without cell body apoptosis (A) Tuj1 immunostaining and corresponding binarized images of axons of E15 DRG neurons grown in compartmented cultures treated with 30 nM paclitaxel or DMSO vehicle control for 24 hours to cell body (left) or distal axon (right) compartments; scale bar = 40 µm. Red boxes outline regions shown at higher magnification in the center panels. Features characteristic of degenerating axons are seen: red arrowheads indicate a “beads on a string” appearance and the red lines indicate interruptions in Tuj1 continuity. (B) Quantification of axonal degeneration: ratio of area of fragmented axons to total axon area (degeneration index); *p

    Article Snippet: Paclitaxel was prepared as 1 part 6 mg/mL paclitaxel stock solution diluted in vehicle (1:1 v/v Cremophor EL [EMD Millipore] and dehydrated ethanol) and 2 parts sterile saline and injected at 10 µL/g.

    Techniques: Immunostaining

    Activation of apoptotic pathway by gefitinib and chloroquine in PC-9/wt and PC-9/gef ells. PC-9/wt, PC-9/gefB4 and PC-9/gefE3 cells were treated with gefitinib (100 nM) and chloroquine (CQ, 10 μM) for 24 h. Total protein of treated cells was harvested. Procaspase 3, active caspase 3, PARP cleavage levels was analyzed with Western blot assay. Each lane contained 30 μg protein for all experiments. Results were repeated in independent experiments.

    Journal: PLoS ONE

    Article Title: Chloroquine Enhances Gefitinib Cytotoxicity in Gefitinib-Resistant Nonsmall Cell Lung Cancer Cells

    doi: 10.1371/journal.pone.0119135

    Figure Lengend Snippet: Activation of apoptotic pathway by gefitinib and chloroquine in PC-9/wt and PC-9/gef ells. PC-9/wt, PC-9/gefB4 and PC-9/gefE3 cells were treated with gefitinib (100 nM) and chloroquine (CQ, 10 μM) for 24 h. Total protein of treated cells was harvested. Procaspase 3, active caspase 3, PARP cleavage levels was analyzed with Western blot assay. Each lane contained 30 μg protein for all experiments. Results were repeated in independent experiments.

    Article Snippet: The primary antibodies included microtubule-associated protein 1 light chain 3 (LC3; Cell Signaling Technology, Beverly, MA, U.S.A., #2775), caspase 3 (Cell Signaling Technology, #9668), and PARP (Cell Signaling Technology, #9542), α-tubulin (Cell Signaling Technology, #2144) and β-actin antibody (Millipore, Bedford, MA, U.S.A.).

    Techniques: Activation Assay, Western Blot