Journal: Cancer science

Article Title: Coordinate suppression of Sdpr and Fhl1 expression in tumors of the breast, kidney, and prostate.

doi: 10.1111/j.1349-7006.2008.00816.x

Figure Lengend Snippet: Fig. 1. Fhl1 and Sdpr expression in normal epi- thelium and tumors from human breast, kidney, and prostate. Immunohistochemical staining was performed on normal and tumor human tissue. Fhl1 and Sdpr staining was evident in normal epithelial cells of the breast, kidney, and prostate. In contrast, both Fhl1 and Sdpr expression were suppressed in infiltrating mammary duct carcinoma, renal cell carcinoma, and prostate adenocarcinoma. (bar = 60 microns).

Article Snippet: Slides containing 15 infiltrating duct carcinoma specimens with normal adjacent tissue from breast cancer patients (IMH-371, Imgenex), nine prostate adenocarcinoma specimens with adjacent normal tissue and an additional unmatched 41 prostate adenocarcinoma specimens (IMH-303, Imgenex), and nine renal cell carcinoma specimens with adjacent normal tissue and an additional unmatched 31 cell carcinoma specimens (IMH-313, Imgenex) were examined by immunohistochemistry as previously described. (19) Briefly, after removal of paraffin with xylene and rehydration through a graded series of decreasing concentrations of ethanol, protein antigenicity was enhanced by microwaving sections in Target Buffer (Dako, Carpenturia, CA, USA) for 2 min.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Cancer Research

Article Title: Blocking Platelet-Derived Growth Factor-D/Platelet-Derived Growth Factor Receptor β Signaling Inhibits Human Renal Cell Carcinoma Progression in an Orthotopic Mouse Model

doi: 10.1158/0008-5472.can-04-4313

Figure Lengend Snippet: Figure 1. PDGF-D is overexpressed in human renal cell carcinoma samples. Normal kidney tissue in the tissue microarray stains with anti–PDGF-D antibody in collecting tubes (A-D). In clear cell type (E and G), granular cell type (F), and granular and clear cell type (H) tumor tissues, PDGF-D stains strongly and homogenously. The arrow indicates positive staining. I, isotype control staining of the tumor tissues. Antigoat immunoglobulin stains negatively of the tumor tissues. Bar, 50 Am.

Article Snippet: Tissue microarray slides bearing 50 human renal cell carcinoma tumor sections and nine normal human kidney sections were purchased from Imgenex (San Diego, CA).

Techniques: Microarray, Staining, Control