Journal: Diagnostic Pathology

Article Title: Coexistence of early microinvasive endometrioid adenocarcinoma and CIN3 in the uterine cervix in a 32-year-old Japanese woman

doi: 10.1186/1746-1596-6-51

Figure Lengend Snippet: Immunohistochemical reagents and results

Article Snippet: Pancytokeratin , CAM5.2 , Beckton-Dickinson, CA, USA , ++ , +.

Techniques: Immunohistochemical staining

Journal:

Article Title: Villin Enhances Hepatocyte Growth Factor-induced Actin Cytoskeleton Remodeling in Epithelial Cells

doi: 10.1091/mbc.E03-02-0091

Figure Lengend Snippet: Cellular models. (A) Primary enterocytes from vil+/+ and vil-/- mice were cultured for 6 d. Epithelial islets were characterized using F-actin (red) (a) and villin (green) (b) double staining. Cytokeratin (green) labeling was used to check the epithelial origin of enterocytes islets (c). (B) Tightly controlled expression of villin by doxycycline (dox) treatment in MDCK cells. Cells grown in presence of the indicated doxycycline concentrations were separated into vil+/+ cells, grown in absence of the antibiotic, and vil-/- cells, grown in presence of doxycycline. Villin expression repression and induction was evaluated by Western blotting. This figure shows that villin induction is efficient when the cells are routinely grown in presence of 0.5 μg/μl doxyxycline (a). However, to avoid leaky expression during repression, the cells are shifted to 2 μg/μl doxycycline. Control of villin expression was also tested by immunofluorescence. Actin was labeled with phalloidin TRITC (b) and villin by using a mAb (c). Bar, 10 μm.

Article Snippet: F-Actin was stained with tetramethylrhodamine B isothiocyanate (TRITC)-phalloidin (1:350, Sigma-Aldrich), villin with a mouse monoclonal antibody (mAb) ID 2 C 3 (1:500, Dudouet et al ., 1987 ), and keratin with an anti-cytokeratin mAb (A45-B/B3) (1:200; ChromaVision Medical Systems).

Techniques: Cell Culture, Double Staining, Labeling, Expressing, Western Blot, Immunofluorescence