page Search Results


zr small rna page recovery kit  (Zymo Research)
96
Zymo Research zr small rna page recovery kit
Zr Small Rna Page Recovery Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini protean electrophoresis module assembly  (Bio-Rad)
96
Bio-Rad mini protean electrophoresis module assembly
Mini Protean Electrophoresis Module Assembly, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resolving gel buffer for page  (Bio-Rad)
93
Bio-Rad resolving gel buffer for page
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Resolving Gel Buffer For Page, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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native polyacrylamide gel  (Bio-Rad)
95
Bio-Rad native polyacrylamide gel
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Native Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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coomassie stain solution  (Rockland Immunochemicals)
92
Rockland Immunochemicals coomassie stain solution
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Coomassie Stain Solution, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds polyacrylamide gel electrophoresis  (Rockland Immunochemicals)
93
Rockland Immunochemicals sds polyacrylamide gel electrophoresis
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Sds Polyacrylamide Gel Electrophoresis, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibody  (Bio X Cell)
93
Bio X Cell monoclonal antibody
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Monoclonal Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibody - by Bioz Stars, 2026-05
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sds page gel preparation kit  (Beyotime)
99
Beyotime sds page gel preparation kit
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Sds Page Gel Preparation Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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barley thioredoxin h isoforms  (ProSci Incorporated)
90
ProSci Incorporated barley thioredoxin h isoforms
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Barley Thioredoxin H Isoforms, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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0318prestained sds page standards  (Bio-Rad)
96
Bio-Rad 0318prestained sds page standards
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
0318prestained Sds Page Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna transfection page 4 18  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology sirna transfection page 4 18
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Sirna Transfection Page 4 18, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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beyogeltm plus precast page gel  (Beyotime)
99
Beyotime beyogeltm plus precast page gel
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Beyogeltm Plus Precast Page Gel, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing gel electrophoresis with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).

Journal: Nature

Article Title: SIGLEC12 mediates plasma membrane rupture during necroptotic cell death

doi: 10.1038/s41586-025-09741-1

Figure Lengend Snippet: a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing gel electrophoresis with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).

Article Snippet: The following reagents were used in this study: SM-164 (S7089), Nec-1s (S8641), GSK′872 (S8465), NSA (S8251), emricasan (S7775) and erastin (S7242) from SelleckChem; etoposide (E1383), α-ketoglutarate (349631), lipopolysaccharide (L4391), luminol (A8511), p -coumaric acid (C9008) and anti-FLAG M2 agarose beads (M8823) from Sigma-Aldrich; nigericin (11437) from the Cayman Chemical Company; Lipofectamine 3000 (L3000015), SuperSignal West Atto Ultimate Sensitivity Substrate (A38556) and Prolong Diamond Antifade Mountant with DAPI ( P36966 ) from Thermo Fisher Scientific; polyethylenimine (PEI; 24765-100) from Kyfora Bio; polybrene (TR-1003) from EMD Millipore; and 10X Tris/Glycine/SDS Electrophoresis Buffer (1610772), Tween 20 (1610781), Stacking Gel Buffer for PAGE (1610799), Resolving Gel Buffer for PAGE (1610798), Precision Plus Protein Dual Color Standards (1610394) and nitrocellulose membrane (1620115) from Bio-Rad.

Techniques: Nucleic Acid Electrophoresis, shRNA, Fluorescence, Confocal Microscopy, Electron Microscopy, Western Blot, Two Tailed Test