padvantage Search Results


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Padvantage Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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padvantage-δnaei ( 28 )  (Promega)
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Restoration of miR-27a/b-mediated inhibition of Ad infection by overexpression of VA-RNAs. (A) HeLa cells were transfected with a control plasmid <t>(ΔNaeI;</t> <t>pAdVAntage-ΔNaeI)</t> or a VA-RNA-expressing plasmid (pAdVAntage). After 48 h of incubation, the copy numbers of miR-27a/b in the cells were determined by quantitative RT-PCR analysis. (B and C) HeLa cells were transfected with a pre-miR-27a- or -b-expressing plasmid (pHM5-U6-pre-miR-27a or -b) (B) or with siRNA at 50 nM (C), followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA were determined by quantitative PCR analysis. (D) HeLa cells were cotransfected with pHM5-U6-pre-miR-27a or -b and pAdVAntage, followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA in the cells were determined. The data are expressed as means and SD (n = 4). *, P < 0.05; **, P < 0.01.
Padvantage δnaei ( 28 ), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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padvantage vector containing vai vaii genes  (Promega)
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Promega padvantage vector containing vai vaii genes
Restoration of miR-27a/b-mediated inhibition of Ad infection by overexpression of VA-RNAs. (A) HeLa cells were transfected with a control plasmid <t>(ΔNaeI;</t> <t>pAdVAntage-ΔNaeI)</t> or a VA-RNA-expressing plasmid (pAdVAntage). After 48 h of incubation, the copy numbers of miR-27a/b in the cells were determined by quantitative RT-PCR analysis. (B and C) HeLa cells were transfected with a pre-miR-27a- or -b-expressing plasmid (pHM5-U6-pre-miR-27a or -b) (B) or with siRNA at 50 nM (C), followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA were determined by quantitative PCR analysis. (D) HeLa cells were cotransfected with pHM5-U6-pre-miR-27a or -b and pAdVAntage, followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA in the cells were determined. The data are expressed as means and SD (n = 4). *, P < 0.05; **, P < 0.01.
Padvantage Vector Containing Vai Vaii Genes, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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padvantage™ vector dna  (Promega)
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Promega padvantage™ vector dna
Restoration of miR-27a/b-mediated inhibition of Ad infection by overexpression of VA-RNAs. (A) HeLa cells were transfected with a control plasmid <t>(ΔNaeI;</t> <t>pAdVAntage-ΔNaeI)</t> or a VA-RNA-expressing plasmid (pAdVAntage). After 48 h of incubation, the copy numbers of miR-27a/b in the cells were determined by quantitative RT-PCR analysis. (B and C) HeLa cells were transfected with a pre-miR-27a- or -b-expressing plasmid (pHM5-U6-pre-miR-27a or -b) (B) or with siRNA at 50 nM (C), followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA were determined by quantitative PCR analysis. (D) HeLa cells were cotransfected with pHM5-U6-pre-miR-27a or -b and pAdVAntage, followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA in the cells were determined. The data are expressed as means and SD (n = 4). *, P < 0.05; **, P < 0.01.
Padvantage™ Vector Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Restoration of miR-27a/b-mediated inhibition of Ad infection by overexpression of VA-RNAs. (A) HeLa cells were transfected with a control plasmid (ΔNaeI; pAdVAntage-ΔNaeI) or a VA-RNA-expressing plasmid (pAdVAntage). After 48 h of incubation, the copy numbers of miR-27a/b in the cells were determined by quantitative RT-PCR analysis. (B and C) HeLa cells were transfected with a pre-miR-27a- or -b-expressing plasmid (pHM5-U6-pre-miR-27a or -b) (B) or with siRNA at 50 nM (C), followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA were determined by quantitative PCR analysis. (D) HeLa cells were cotransfected with pHM5-U6-pre-miR-27a or -b and pAdVAntage, followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA in the cells were determined. The data are expressed as means and SD (n = 4). *, P < 0.05; **, P < 0.01.

Journal: Journal of Virology

Article Title: MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2

doi: 10.1128/JVI.00159-17

Figure Lengend Snippet: Restoration of miR-27a/b-mediated inhibition of Ad infection by overexpression of VA-RNAs. (A) HeLa cells were transfected with a control plasmid (ΔNaeI; pAdVAntage-ΔNaeI) or a VA-RNA-expressing plasmid (pAdVAntage). After 48 h of incubation, the copy numbers of miR-27a/b in the cells were determined by quantitative RT-PCR analysis. (B and C) HeLa cells were transfected with a pre-miR-27a- or -b-expressing plasmid (pHM5-U6-pre-miR-27a or -b) (B) or with siRNA at 50 nM (C), followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA were determined by quantitative PCR analysis. (D) HeLa cells were cotransfected with pHM5-U6-pre-miR-27a or -b and pAdVAntage, followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA in the cells were determined. The data are expressed as means and SD (n = 4). *, P < 0.05; **, P < 0.01.

Article Snippet: The sequences of the primers used in this study are given in . pAdVAntage-ΔNaeI ( 28 ), a control plasmid lacking VA-RNA expression, was previously constructed using pAdVAntage (Promega, Madison, WI), which encodes VA-RNA I and II under the control of an endogenous RNA polymerase III promoter.

Techniques: Inhibition, Infection, Over Expression, Transfection, Plasmid Preparation, Expressing, Incubation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction