Journal: Translational Psychiatry
Article Title: Label-free, live optical imaging of reprogrammed bipolar disorder patient-derived cells reveals a functional correlate of lithium responsiveness
Figure Lengend Snippet: Generation of BD patient-derived iNLCs and BIND imaging. ( a ) Fibroblasts transduced with miR-9/9*-124, NEUROD2 , ASCL1 and MYT1L undergo selection 3 days after lentiviral infection. RFP expression indicates miR-9/9*-124 expression. Morphological changes occur while cells are undergoing selection. At 14 days posttransduction, cells have elongated processes resembling neurite outgrowth. ( b ) Nanostructured photonic crystal biosensors form the bottom surface of a 384-well plate onto which cells can adhere. Collimated broadband light is projected onto the biosensor, and the resulting resonant reflected light is collected and recorded by an imaging spectrometer, illustrated in c . ( d ) An overlay of the RFP indicator and BIND scan show that detailed morphology of cells can be captured by the BIND scan and is similar to the morphology seen by fluorescent light microscopy of the RFP indicator. ( e ) Incubated BIND Scanner allows long-term time-lapse imaging of iNLCs. BD, bipolar disorder; iNLC, induced neuronal-like cell; RFP, red fluorescent protein.
Article Snippet: We also acknowledge Bob Weinberg and Didier Trono for the use of lentiviral packaging plasmids deposited with Addgene.
Techniques: Derivative Assay, Imaging, Transduction, Selection, Infection, Expressing, Light Microscopy, Incubation