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  • 99
    Millipore lentiviral packaging plasmid
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Lentiviral Packaging Plasmid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral packaging plasmid/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    lentiviral packaging plasmid - by Bioz Stars, 2020-08
    99/100 stars
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    93
    Addgene inc packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/Addgene inc
    Average 93 stars, based on 1524 article reviews
    Price from $9.99 to $1999.99
    packaging plasmids - by Bioz Stars, 2020-08
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    93
    Thermo Fisher packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3044 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 3044 article reviews
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    92
    Genecopoeia packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 92/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/Genecopoeia
    Average 92 stars, based on 88 article reviews
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    92
    ABM Inc packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by ABM Inc, used in various techniques. Bioz Stars score: 92/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 74 article reviews
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    packaging plasmids - by Bioz Stars, 2020-08
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    92
    GenScript packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/GenScript
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    packaging plasmids - by Bioz Stars, 2020-08
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    92
    Millipore packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/Millipore
    Average 92 stars, based on 228 article reviews
    Price from $9.99 to $1999.99
    packaging plasmids - by Bioz Stars, 2020-08
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    91
    Shanghai Genechem packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by Shanghai Genechem, used in various techniques. Bioz Stars score: 91/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/Shanghai Genechem
    Average 91 stars, based on 53 article reviews
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    packaging plasmids - by Bioz Stars, 2020-08
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    93
    SIRION Biotech packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by SIRION Biotech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/SIRION Biotech
    Average 93 stars, based on 10 article reviews
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    packaging plasmids - by Bioz Stars, 2020-08
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    92
    Shanghai GenePharma packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/Shanghai GenePharma
    Average 92 stars, based on 35 article reviews
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    packaging plasmids - by Bioz Stars, 2020-08
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    92
    Cell Genesys packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by Cell Genesys, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/Cell Genesys
    Average 92 stars, based on 5 article reviews
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    packaging plasmids - by Bioz Stars, 2020-08
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    93
    OriGene packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/OriGene
    Average 93 stars, based on 203 article reviews
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    packaging plasmids - by Bioz Stars, 2020-08
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    93
    Cellecta packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by Cellecta, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/Cellecta
    Average 93 stars, based on 4 article reviews
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    packaging plasmids - by Bioz Stars, 2020-08
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    92
    GenePharma Company packaging plasmids
    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA <t>lentiviral</t> library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.
    Packaging Plasmids, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 92/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids/product/GenePharma Company
    Average 92 stars, based on 90 article reviews
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    packaging plasmids - by Bioz Stars, 2020-08
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    93
    Addgene inc packaging plasmid pspax2
    Validation of the integration-defective phenotype of vector particles generated with the aid of packaging construct <t>psPAX2.IN</t> D116N . (A) Genetic composition of the HIV-1-based LVs and IDLVs generated for and used in the current study. Lentiviral vectors
    Packaging Plasmid Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmid pspax2/product/Addgene inc
    Average 93 stars, based on 1559 article reviews
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    packaging plasmid pspax2 - by Bioz Stars, 2020-08
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    90
    Addgene inc pax2 packaging plasmid
    Validation of the integration-defective phenotype of vector particles generated with the aid of packaging construct <t>psPAX2.IN</t> D116N . (A) Genetic composition of the HIV-1-based LVs and IDLVs generated for and used in the current study. Lentiviral vectors
    Pax2 Packaging Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pax2 packaging plasmid/product/Addgene inc
    Average 90 stars, based on 25 article reviews
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    pax2 packaging plasmid - by Bioz Stars, 2020-08
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    85
    Thermo Fisher virapower packaging plasmids
    Validation of the integration-defective phenotype of vector particles generated with the aid of packaging construct <t>psPAX2.IN</t> D116N . (A) Genetic composition of the HIV-1-based LVs and IDLVs generated for and used in the current study. Lentiviral vectors
    Virapower Packaging Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 27 article reviews
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    virapower packaging plasmids - by Bioz Stars, 2020-08
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    90
    Genecopoeia lentiviral packaging plasmids
    Exogenous overexpression of IFIT2 enhances sensitivity of K562 cells to curcumin (A) FIT2 gene-containing <t>lentiviral</t> infected K562 cells and detected the expression of IFIT2 by RT-PCR and western blot; (B) the expression of c-myc and skp2 were analyzed by RT-PCR after overexpression of IFIT2; (C) K562, K562-Vector and K562-IFIT2 were treated with or without 10μmol/L for 24 h, Annexin-V/PtdIns double-staining assay was used to detect percentage of apoptotic cells. *P
    Lentiviral Packaging Plasmids, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral packaging plasmids/product/Genecopoeia
    Average 90 stars, based on 14 article reviews
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    lentiviral packaging plasmids - by Bioz Stars, 2020-08
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    93
    System Biosciences Inc ppackh1 packaging plasmid
    Exogenous overexpression of IFIT2 enhances sensitivity of K562 cells to curcumin (A) FIT2 gene-containing <t>lentiviral</t> infected K562 cells and detected the expression of IFIT2 by RT-PCR and western blot; (B) the expression of c-myc and skp2 were analyzed by RT-PCR after overexpression of IFIT2; (C) K562, K562-Vector and K562-IFIT2 were treated with or without 10μmol/L for 24 h, Annexin-V/PtdIns double-staining assay was used to detect percentage of apoptotic cells. *P
    Ppackh1 Packaging Plasmid, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ppackh1 packaging plasmid/product/System Biosciences Inc
    Average 93 stars, based on 19 article reviews
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    ppackh1 packaging plasmid - by Bioz Stars, 2020-08
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    91
    Addgene inc lentiviral packaging plasmids
    Generation of BD patient-derived iNLCs and BIND imaging. ( a ) Fibroblasts transduced with miR-9/9*-124, NEUROD2 , ASCL1 and MYT1L undergo selection 3 days after <t>lentiviral</t> infection. RFP expression indicates miR-9/9*-124 expression. Morphological changes occur while cells are undergoing selection. At 14 days posttransduction, cells have elongated processes resembling neurite outgrowth. ( b ) Nanostructured photonic crystal biosensors form the bottom surface of a 384-well plate onto which cells can adhere. Collimated broadband light is projected onto the biosensor, and the resulting resonant reflected light is collected and recorded by an imaging spectrometer, illustrated in c . ( d ) An overlay of the RFP indicator and BIND scan show that detailed morphology of cells can be captured by the BIND scan and is similar to the morphology seen by fluorescent light microscopy of the RFP indicator. ( e ) Incubated BIND Scanner allows long-term time-lapse imaging of iNLCs. BD, bipolar disorder; iNLC, induced neuronal-like cell; RFP, red fluorescent protein.
    Lentiviral Packaging Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral packaging plasmids/product/Addgene inc
    Average 91 stars, based on 181 article reviews
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    lentiviral packaging plasmids - by Bioz Stars, 2020-08
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    89
    Addgene inc packaging plasmids pvsvg
    Generation of BD patient-derived iNLCs and BIND imaging. ( a ) Fibroblasts transduced with miR-9/9*-124, NEUROD2 , ASCL1 and MYT1L undergo selection 3 days after <t>lentiviral</t> infection. RFP expression indicates miR-9/9*-124 expression. Morphological changes occur while cells are undergoing selection. At 14 days posttransduction, cells have elongated processes resembling neurite outgrowth. ( b ) Nanostructured photonic crystal biosensors form the bottom surface of a 384-well plate onto which cells can adhere. Collimated broadband light is projected onto the biosensor, and the resulting resonant reflected light is collected and recorded by an imaging spectrometer, illustrated in c . ( d ) An overlay of the RFP indicator and BIND scan show that detailed morphology of cells can be captured by the BIND scan and is similar to the morphology seen by fluorescent light microscopy of the RFP indicator. ( e ) Incubated BIND Scanner allows long-term time-lapse imaging of iNLCs. BD, bipolar disorder; iNLC, induced neuronal-like cell; RFP, red fluorescent protein.
    Packaging Plasmids Pvsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 89/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmids pvsvg/product/Addgene inc
    Average 89 stars, based on 288 article reviews
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    packaging plasmids pvsvg - by Bioz Stars, 2020-08
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    88
    Addgene inc packaging plasmid ppax2
    Generation of BD patient-derived iNLCs and BIND imaging. ( a ) Fibroblasts transduced with miR-9/9*-124, NEUROD2 , ASCL1 and MYT1L undergo selection 3 days after <t>lentiviral</t> infection. RFP expression indicates miR-9/9*-124 expression. Morphological changes occur while cells are undergoing selection. At 14 days posttransduction, cells have elongated processes resembling neurite outgrowth. ( b ) Nanostructured photonic crystal biosensors form the bottom surface of a 384-well plate onto which cells can adhere. Collimated broadband light is projected onto the biosensor, and the resulting resonant reflected light is collected and recorded by an imaging spectrometer, illustrated in c . ( d ) An overlay of the RFP indicator and BIND scan show that detailed morphology of cells can be captured by the BIND scan and is similar to the morphology seen by fluorescent light microscopy of the RFP indicator. ( e ) Incubated BIND Scanner allows long-term time-lapse imaging of iNLCs. BD, bipolar disorder; iNLC, induced neuronal-like cell; RFP, red fluorescent protein.
    Packaging Plasmid Ppax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packaging plasmid ppax2/product/Addgene inc
    Average 88 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    packaging plasmid ppax2 - by Bioz Stars, 2020-08
    88/100 stars
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    90
    Addgene inc ecotropic packaging plasmids
    Generation of BD patient-derived iNLCs and BIND imaging. ( a ) Fibroblasts transduced with miR-9/9*-124, NEUROD2 , ASCL1 and MYT1L undergo selection 3 days after <t>lentiviral</t> infection. RFP expression indicates miR-9/9*-124 expression. Morphological changes occur while cells are undergoing selection. At 14 days posttransduction, cells have elongated processes resembling neurite outgrowth. ( b ) Nanostructured photonic crystal biosensors form the bottom surface of a 384-well plate onto which cells can adhere. Collimated broadband light is projected onto the biosensor, and the resulting resonant reflected light is collected and recorded by an imaging spectrometer, illustrated in c . ( d ) An overlay of the RFP indicator and BIND scan show that detailed morphology of cells can be captured by the BIND scan and is similar to the morphology seen by fluorescent light microscopy of the RFP indicator. ( e ) Incubated BIND Scanner allows long-term time-lapse imaging of iNLCs. BD, bipolar disorder; iNLC, induced neuronal-like cell; RFP, red fluorescent protein.
    Ecotropic Packaging Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecotropic packaging plasmids/product/Addgene inc
    Average 90 stars, based on 3 article reviews
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    ecotropic packaging plasmids - by Bioz Stars, 2020-08
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    93
    TaKaRa ecotropic packaging plasmids
    Generation of BD patient-derived iNLCs and BIND imaging. ( a ) Fibroblasts transduced with miR-9/9*-124, NEUROD2 , ASCL1 and MYT1L undergo selection 3 days after <t>lentiviral</t> infection. RFP expression indicates miR-9/9*-124 expression. Morphological changes occur while cells are undergoing selection. At 14 days posttransduction, cells have elongated processes resembling neurite outgrowth. ( b ) Nanostructured photonic crystal biosensors form the bottom surface of a 384-well plate onto which cells can adhere. Collimated broadband light is projected onto the biosensor, and the resulting resonant reflected light is collected and recorded by an imaging spectrometer, illustrated in c . ( d ) An overlay of the RFP indicator and BIND scan show that detailed morphology of cells can be captured by the BIND scan and is similar to the morphology seen by fluorescent light microscopy of the RFP indicator. ( e ) Incubated BIND Scanner allows long-term time-lapse imaging of iNLCs. BD, bipolar disorder; iNLC, induced neuronal-like cell; RFP, red fluorescent protein.
    Ecotropic Packaging Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA lentiviral library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.

    Journal: Retrovirology

    Article Title: Genome-wide shRNA screening identifies host factors involved in early endocytic events for HIV-1-induced CD4 down-regulation

    doi: 10.1186/s12977-014-0118-4

    Figure Lengend Snippet: Schematic representation of the genome-wide shRNA screening workflow. HeLa CD4++ cells were transduced with a pooled shRNA lentiviral library. Productively transduced cells were selected with puromycin and transduced with a retroviral vector for the expression of HIV-1 Nef and eGFP as a reporter gene. Cells that maintained high CD4 surface levels despite the expression of Nef (Nef(eGFP) + CD4 hi , red box) and the Nef(eGFP) + CD4 low population (green box) were sorted by FACS. The shRNA hairpins present in the cDNA obtained from the two sorted populations were amplified by nested PCR with biotinylated primers. The samples obtained were hybridized on different Affymetrix U133 v2-plus microarrays and the results compared. shRNA sequences enriched in the Nef(eGFP) + CD4 hi cells were selected and further analyzed with pathway analysis tools to obtain a final list of 55 genes to be further validated together with 20 genes selected from literature.

    Article Snippet: MISSION® Lentiviral Packaging Mix containing lentiviral packaging plasmid and the VSV envelope plasmid were purchased as a pre-made mix from Sigma Aldrich.

    Techniques: Genome Wide, shRNA, Transduction, Plasmid Preparation, Expressing, FACS, Amplification, Nested PCR

    Validation of the integration-defective phenotype of vector particles generated with the aid of packaging construct psPAX2.IN D116N . (A) Genetic composition of the HIV-1-based LVs and IDLVs generated for and used in the current study. Lentiviral vectors

    Journal: Human Gene Therapy

    Article Title: Histone Deacetylase Inhibition Activates Transgene Expression from Integration-Defective Lentiviral Vectors in Dividing and Non-Dividing Cells

    doi: 10.1089/hum.2012.069

    Figure Lengend Snippet: Validation of the integration-defective phenotype of vector particles generated with the aid of packaging construct psPAX2.IN D116N . (A) Genetic composition of the HIV-1-based LVs and IDLVs generated for and used in the current study. Lentiviral vectors

    Article Snippet: The second-generation packaging plasmid psPAX2 was provided by Didier Trono (Addgene plasmid 12260; Cambridge, MA), whilst the third-generation packaging plasmid pLP1, the HIV-1 rev expression plasmid pLP2, and the vesicular stomatitis glycoprotein-G (VSV-G) pseudotyping construct pLP/VSVG are from Invitrogen.

    Techniques: Plasmid Preparation, Generated, Construct

    Exogenous overexpression of IFIT2 enhances sensitivity of K562 cells to curcumin (A) FIT2 gene-containing lentiviral infected K562 cells and detected the expression of IFIT2 by RT-PCR and western blot; (B) the expression of c-myc and skp2 were analyzed by RT-PCR after overexpression of IFIT2; (C) K562, K562-Vector and K562-IFIT2 were treated with or without 10μmol/L for 24 h, Annexin-V/PtdIns double-staining assay was used to detect percentage of apoptotic cells. *P

    Journal: Cancer Biology & Therapy

    Article Title: Curcumin induces apoptosis in human leukemic cell lines through an IFIT2-dependent pathway

    doi: 10.1080/15384047.2016.1276129

    Figure Lengend Snippet: Exogenous overexpression of IFIT2 enhances sensitivity of K562 cells to curcumin (A) FIT2 gene-containing lentiviral infected K562 cells and detected the expression of IFIT2 by RT-PCR and western blot; (B) the expression of c-myc and skp2 were analyzed by RT-PCR after overexpression of IFIT2; (C) K562, K562-Vector and K562-IFIT2 were treated with or without 10μmol/L for 24 h, Annexin-V/PtdIns double-staining assay was used to detect percentage of apoptotic cells. *P

    Article Snippet: The lentiviral expression vector and the lentiviral packaging plasmids were transfected into 293T cells by EndoFectin™ (GeneCopoeia, Inc.

    Techniques: Over Expression, Infection, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Plasmid Preparation, Double Staining

    Generation of BD patient-derived iNLCs and BIND imaging. ( a ) Fibroblasts transduced with miR-9/9*-124, NEUROD2 , ASCL1 and MYT1L undergo selection 3 days after lentiviral infection. RFP expression indicates miR-9/9*-124 expression. Morphological changes occur while cells are undergoing selection. At 14 days posttransduction, cells have elongated processes resembling neurite outgrowth. ( b ) Nanostructured photonic crystal biosensors form the bottom surface of a 384-well plate onto which cells can adhere. Collimated broadband light is projected onto the biosensor, and the resulting resonant reflected light is collected and recorded by an imaging spectrometer, illustrated in c . ( d ) An overlay of the RFP indicator and BIND scan show that detailed morphology of cells can be captured by the BIND scan and is similar to the morphology seen by fluorescent light microscopy of the RFP indicator. ( e ) Incubated BIND Scanner allows long-term time-lapse imaging of iNLCs. BD, bipolar disorder; iNLC, induced neuronal-like cell; RFP, red fluorescent protein.

    Journal: Translational Psychiatry

    Article Title: Label-free, live optical imaging of reprogrammed bipolar disorder patient-derived cells reveals a functional correlate of lithium responsiveness

    doi: 10.1038/tp.2014.72

    Figure Lengend Snippet: Generation of BD patient-derived iNLCs and BIND imaging. ( a ) Fibroblasts transduced with miR-9/9*-124, NEUROD2 , ASCL1 and MYT1L undergo selection 3 days after lentiviral infection. RFP expression indicates miR-9/9*-124 expression. Morphological changes occur while cells are undergoing selection. At 14 days posttransduction, cells have elongated processes resembling neurite outgrowth. ( b ) Nanostructured photonic crystal biosensors form the bottom surface of a 384-well plate onto which cells can adhere. Collimated broadband light is projected onto the biosensor, and the resulting resonant reflected light is collected and recorded by an imaging spectrometer, illustrated in c . ( d ) An overlay of the RFP indicator and BIND scan show that detailed morphology of cells can be captured by the BIND scan and is similar to the morphology seen by fluorescent light microscopy of the RFP indicator. ( e ) Incubated BIND Scanner allows long-term time-lapse imaging of iNLCs. BD, bipolar disorder; iNLC, induced neuronal-like cell; RFP, red fluorescent protein.

    Article Snippet: We also acknowledge Bob Weinberg and Didier Trono for the use of lentiviral packaging plasmids deposited with Addgene.

    Techniques: Derivative Assay, Imaging, Transduction, Selection, Infection, Expressing, Light Microscopy, Incubation