Journal: Journal of gastroenterology

Article Title: Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium.

doi: 10.1007/s00535-020-01695-7

Figure Lengend Snippet: Fig. 1 Induction of EECs from hiPSCs. a A schematic diagram of the experiment to examine the conditions underlying the differentiation of foregut (FG) into dorsal anterior foregut (dAFG). b An expression analysis of SOX2 and p63 at Day 13 by semi-quantitative RT-PCR. GAPDH was used as an endogenous control. c Immunostaining of SOX2 (green), p63 (red) and Hoechst (blue) at Day 13. Representative fluorescence is shown. Scale bars, 50 lm. d A schematic diagram of the experiment to examine the conditions underlying the differentiation of dAFG into EECs. e Expression analyses of p63, SOX2, CK13, CK5 and PAX9 at Day 21 by semi-quantitative RT-PCR. GAPDH was used as an endogenous control. Total RNA of human normal esophageal tissue was used as a positive control

Article Snippet: The primary antibodies were rabbit anti-SOX2 (ab97959; abcam), rabbit anti-CK13 (ab92551, dilution 1:200; abcam), goat anti-p63 (BAF1916, dilution 1:200; R&D Systems), rat anti-PAX9 (ab28538, dilution 1:200; abcam), goat anti-Ecadherin (AF648, dilution 1:200; R&D Systems), rabbit anti-CK5 (GTX113219, dilution 1:200; GeneTex, Irvine, CA, USA), rabbit anti-Claudin4 (ab210796, dilution 1:200; abcam) and rabbit anti-Involucrin (ab53112, dilution 1:200; abcam).

Techniques: Expressing, Quantitative RT-PCR, Control, Immunostaining, Positive Control

Journal: Journal of gastroenterology

Article Title: Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium.

doi: 10.1007/s00535-020-01695-7

Figure Lengend Snippet: Fig. 2 The expression of esophageal epithelial marker proteins in the hiPSC-derived cells and fetal mouse esophagus at E17.5. a A schematic representation of the stepwise differentiation protocol and marker genes at each step. b Immunostaining of the SOX2, p63, CK5, E-cadherin, CK13 and PAX9 at Day 24 in the derivatives of hiPSCs (upper panels) and fetal mouse esophagus at E17.5 (lower panels). Scale bars, 20 lm

Article Snippet: The primary antibodies were rabbit anti-SOX2 (ab97959; abcam), rabbit anti-CK13 (ab92551, dilution 1:200; abcam), goat anti-p63 (BAF1916, dilution 1:200; R&D Systems), rat anti-PAX9 (ab28538, dilution 1:200; abcam), goat anti-Ecadherin (AF648, dilution 1:200; R&D Systems), rabbit anti-CK5 (GTX113219, dilution 1:200; GeneTex, Irvine, CA, USA), rabbit anti-Claudin4 (ab210796, dilution 1:200; abcam) and rabbit anti-Involucrin (ab53112, dilution 1:200; abcam).

Techniques: Expressing, Marker, Derivative Assay, Immunostaining

Journal: Journal of gastroenterology

Article Title: Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium.

doi: 10.1007/s00535-020-01695-7

Figure Lengend Snippet: Fig. 5 Treatment with an RARc-specific agonist instead of ATRA also induced stratified layers of cells expressing esophageal epithelial markers. a A schematic diagram of the EEC differentiation protocol with an RARc-specific agonist. b Immunostaining of SOX2, p63, CK5, E-cadherin, CK13 and PAX9 in the derivatives of hiPSCs. Scale bars, 20 lm

Article Snippet: The primary antibodies were rabbit anti-SOX2 (ab97959; abcam), rabbit anti-CK13 (ab92551, dilution 1:200; abcam), goat anti-p63 (BAF1916, dilution 1:200; R&D Systems), rat anti-PAX9 (ab28538, dilution 1:200; abcam), goat anti-Ecadherin (AF648, dilution 1:200; R&D Systems), rabbit anti-CK5 (GTX113219, dilution 1:200; GeneTex, Irvine, CA, USA), rabbit anti-Claudin4 (ab210796, dilution 1:200; abcam) and rabbit anti-Involucrin (ab53112, dilution 1:200; abcam).

Techniques: Expressing, Immunostaining

Journal: eLife

Article Title: Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids

doi: 10.7554/eLife.26575

Figure Lengend Snippet: Primary antibodies DOI: http://dx.doi.org/10.7554/eLife.26575.041

Article Snippet: TP63 , Cell Signaling , 13109 , Rabbit , 1:200 , Yes , Yes (citrate); needs streptavidin-biotin amplification , RRID: AB_2637091.

Techniques: Amplification, Transduction

Journal: Veterinary Ophthalmology

Article Title: Morphological and immunohistochemical characteristics of the equine corneal epithelium

doi: 10.1111/vop.12651

Figure Lengend Snippet: Sources, dilutions, fixations and pretreatments of the antibodies used

Article Snippet: p63 , Potential SC marker , Polyclonal orb214808 , Rabbit , Biorbyt, Cambridge, UK , 1:2500 , f , 0.01 mol/L citrate buffer pH 6.0, 30 min steamer.

Techniques: Cell Differentiation, Marker

Journal: Veterinary Ophthalmology

Article Title: Morphological and immunohistochemical characteristics of the equine corneal epithelium

doi: 10.1111/vop.12651

Figure Lengend Snippet: Semiquantitative analysis of immunohistochemical staining for cytoskeletal proteins, proliferation, differentiation, and stem cell markers. CK 14, p63, and NGF showed a gradual decrease from crypt to center. Statistically significant age differences (*, P < 0.05) were detected in the limbal zone for NGF and in the noncrypt zone for ABCG2. Bars represent mean values from six horses of both age groups and whiskers represent SD.

Article Snippet: p63 , Potential SC marker , Polyclonal orb214808 , Rabbit , Biorbyt, Cambridge, UK , 1:2500 , f , 0.01 mol/L citrate buffer pH 6.0, 30 min steamer.

Techniques: Immunohistochemical staining, Staining

Journal: Veterinary Ophthalmology

Article Title: Morphological and immunohistochemical characteristics of the equine corneal epithelium

doi: 10.1111/vop.12651

Figure Lengend Snippet: Immunohistochemical staining of proposed SC‐associated markers Ki67, p63, NGF, ABCG2, and EGFR. Representative pictures of crypts, limbus, and central corneal epithelium are shown for both foals (left panels) and adult horses (right panels). Potential stem cell markers (p63, NGF, and ABCG2) were present in every examined region with decreasing frequency toward the center. Scale bar =30 µm

Article Snippet: p63 , Potential SC marker , Polyclonal orb214808 , Rabbit , Biorbyt, Cambridge, UK , 1:2500 , f , 0.01 mol/L citrate buffer pH 6.0, 30 min steamer.

Techniques: Immunohistochemical staining, Staining

Journal: Scientific reports

Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells.

doi: 10.1038/s41598-025-96919-2

Figure Lengend Snippet: Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers p63 and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.

Article Snippet: After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), rabbit anti-p63 polyclonal (1:50; 12143-1-AP; Proteintech, USA), mouse anti-CK3 monoclonal (1:50; ab68260; Abcam, UK), and rabbit anti-CK12 monoclonal (1:50; ab185627; Abcam, UK) primary antibodies at 4 °C overnight.

Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control, Expressing, Cell Culture, Staining, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Irradiation Induces Epithelial Cell Unjamming

doi: 10.3389/fcell.2020.00021

Figure Lengend Snippet: Ionizing radiation does not disrupt normal epithelial cell functions. (A) On ALI days 7, 10, and 14, TEER measured 1 h before (pre-exposure, open symbols) and 1 h after (post-exposure, closed symbols) indicates that IR exposure did not reduce TEER. Black lines represent TEER measured from two independent wells (Control A and Control B) of control and red lines represent TEER measured from two independent wells (Irradiated A and Irradiated B) of irradiated cells from one donor. (B) On ALI day 14, the percent change in TEER comparing 1 h pre-, and 1 h post-IR exposure showed no difference between control (white bar) and irradiated (black bar) cells, indicating that IR did not reduce TEER. Error bars represent the standard error of the mean from four independent donors ( n = 4). (C) Methanol treatment used as a positive control for cell death resulted in prominent cell death as indicated by EthD-1 staining (red), whereas neither control nor IR exposure induced cell death (scale bars = 50 μm). Representative images (D) of MUC5AC, FOXJ1, p63α (20X), and β4-tubulin (63X). RT-qPCR (E) showing mRNA expression of MUC5AC, FOXJ1, TEKT1 , and TP63 indicate no differences in cellular differentiation between control and irradiated cells. Scale bar = 50 μm (top 8 panels), 20 μm (bottom 2 panels) in (D) and error bars represent the standard deviation from two representative donors ( n = 2) in (E) .

Article Snippet: To observe the differentiation of the ALI cultures, we stained fixed cells with primary antibodies for MUC5AC (45M1, ThermoFisher Scientific), FOXJ1 (2A5, ThermoFisher Scientific), β4-tubulin (ONS, Millipore Sigma), and p63α (D2K8X, Cell Signaling Technology).

Techniques: Control, Irradiation, Positive Control, Staining, Quantitative RT-PCR, Expressing, Cell Differentiation, Standard Deviation

Journal: Cell reports

Article Title: TP63-Mediated Enhancer Reprogramming Drives the Squamous Subtype of Pancreatic Ductal Adenocarcinoma

doi: 10.1016/j.celrep.2018.10.051

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-TP63 (for WB, IHC and ChIP) , Cell Signaling , Cat# 39692; RRID: N/A.

Techniques: Plasmid Preparation, Recombinant, Sample Prep, SYBR Green Assay, Reverse Transcription, Purification, Gel Extraction, Cell Viability Assay, Membrane, Microarray, Software