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protoporphyrin ix disodium salt  (Frontier Specialty Chemicals Inc)
90
Frontier Specialty Chemicals Inc protoporphyrin ix disodium salt
Figure 1. The influence of metalloporphyrins on caspase-3- like activity, HO-1 protein expression, and PARP cleavage in intact Jurkat T-lymphocytes. A) Cells were either left un- treated (Co) or treated with Fas ligand (FasL, 100 ng/mL, 16 h). The HO-1 inducer cobalt(III) <t>protoporphyrin-IX</t> (CoPP, 10 M) and the HO-1 inhibitors tin and zinc(II) protoporphyrin-IX (SnPP, ZnPP, 10 M each) were added 30 min before the cells were lysed. Caspase-3-like activity was determined. Bars represent means se of 3 independent experiments performed in triplicates and values of untreated cells were set as 1. ***P 0.001 represents significant differences compared with Co. P 0.001 represents significant differences compared with cells treated with FasL alone. B) To detect HO-1 protein expression by Western blot, cells were either left untreated (Co) or treated with FasL (100 ng/mL, 16 h) or with the different porphyrins (CoPP, SnPP, ZnPP, 10 M each, 30 min). Human umbilical vein endothe- lial cells (HUVEC) treated with CoPP (10 M, 30 min) served as a positive control (P) for induction of HO-1 protein expression. One representative blot of three is shown. C) To detect PARP cleavage by Western blot, cells were either left untreated (Co) or treated with FasL (100 ng/mL) alone or together with CoPP (10 M) for 6 h. Experiments were performed 3 times with consistent results and 1 representative blot is shown.
Protoporphyrin Ix Disodium Salt, supplied by Frontier Specialty Chemicals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. The influence of metalloporphyrins on caspase-3- like activity, HO-1 protein expression, and PARP cleavage in intact Jurkat T-lymphocytes. A) Cells were either left un- treated (Co) or treated with Fas ligand (FasL, 100 ng/mL, 16 h). The HO-1 inducer cobalt(III) protoporphyrin-IX (CoPP, 10 M) and the HO-1 inhibitors tin and zinc(II) protoporphyrin-IX (SnPP, ZnPP, 10 M each) were added 30 min before the cells were lysed. Caspase-3-like activity was determined. Bars represent means se of 3 independent experiments performed in triplicates and values of untreated cells were set as 1. ***P 0.001 represents significant differences compared with Co. P 0.001 represents significant differences compared with cells treated with FasL alone. B) To detect HO-1 protein expression by Western blot, cells were either left untreated (Co) or treated with FasL (100 ng/mL, 16 h) or with the different porphyrins (CoPP, SnPP, ZnPP, 10 M each, 30 min). Human umbilical vein endothe- lial cells (HUVEC) treated with CoPP (10 M, 30 min) served as a positive control (P) for induction of HO-1 protein expression. One representative blot of three is shown. C) To detect PARP cleavage by Western blot, cells were either left untreated (Co) or treated with FasL (100 ng/mL) alone or together with CoPP (10 M) for 6 h. Experiments were performed 3 times with consistent results and 1 representative blot is shown.

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Metalloporphyrins inactivate caspase-3 and -8.

doi: 10.1096/fj.04-3259com

Figure Lengend Snippet: Figure 1. The influence of metalloporphyrins on caspase-3- like activity, HO-1 protein expression, and PARP cleavage in intact Jurkat T-lymphocytes. A) Cells were either left un- treated (Co) or treated with Fas ligand (FasL, 100 ng/mL, 16 h). The HO-1 inducer cobalt(III) protoporphyrin-IX (CoPP, 10 M) and the HO-1 inhibitors tin and zinc(II) protoporphyrin-IX (SnPP, ZnPP, 10 M each) were added 30 min before the cells were lysed. Caspase-3-like activity was determined. Bars represent means se of 3 independent experiments performed in triplicates and values of untreated cells were set as 1. ***P 0.001 represents significant differences compared with Co. P 0.001 represents significant differences compared with cells treated with FasL alone. B) To detect HO-1 protein expression by Western blot, cells were either left untreated (Co) or treated with FasL (100 ng/mL, 16 h) or with the different porphyrins (CoPP, SnPP, ZnPP, 10 M each, 30 min). Human umbilical vein endothe- lial cells (HUVEC) treated with CoPP (10 M, 30 min) served as a positive control (P) for induction of HO-1 protein expression. One representative blot of three is shown. C) To detect PARP cleavage by Western blot, cells were either left untreated (Co) or treated with FasL (100 ng/mL) alone or together with CoPP (10 M) for 6 h. Experiments were performed 3 times with consistent results and 1 representative blot is shown.

Article Snippet: Protoporphyrin IX disodium salt was from Frontier Scientific Porphyrin Products (Carnforth/ Lancashire, UK).

Techniques: Activity Assay, Expressing, Western Blot, Positive Control

Figure 4. Metalloporphyrins and protoporphyrin sodium salt influence the activity of recombinant caspase-3. CoPP (0.001–10 M), SnPP (0.001–100 M), ZnPP (0.001–100 M (A), or protoporphyrin-IX disodium salt (PP, 10 M) (C) were added to recombinant caspases-3 (C-3) immediately before measurement of caspase activity. In further experi- ments, CoPP (10 M) was added to recombinant caspase-3 with increasing amounts of the caspase-3 substrate (B). Data represent means se of 3 independent experiments per- formed in triplicates and activity of caspase-3 in the absence of the porphyrins was taken as 100%. ***P 0.001 represents significant differences compared with the respective C-3 group.

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Metalloporphyrins inactivate caspase-3 and -8.

doi: 10.1096/fj.04-3259com

Figure Lengend Snippet: Figure 4. Metalloporphyrins and protoporphyrin sodium salt influence the activity of recombinant caspase-3. CoPP (0.001–10 M), SnPP (0.001–100 M), ZnPP (0.001–100 M (A), or protoporphyrin-IX disodium salt (PP, 10 M) (C) were added to recombinant caspases-3 (C-3) immediately before measurement of caspase activity. In further experi- ments, CoPP (10 M) was added to recombinant caspase-3 with increasing amounts of the caspase-3 substrate (B). Data represent means se of 3 independent experiments per- formed in triplicates and activity of caspase-3 in the absence of the porphyrins was taken as 100%. ***P 0.001 represents significant differences compared with the respective C-3 group.

Article Snippet: Protoporphyrin IX disodium salt was from Frontier Scientific Porphyrin Products (Carnforth/ Lancashire, UK).

Techniques: Activity Assay, Recombinant

Figure 7. Auto-Dock results. Caspase-3 together with 10 putative binding conformations of protoporphyrin (A). Rep- resentation of the energetically most favorable caspase-3/ protoporphyrin complex showing the major protein-ligand interactions (B).

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Metalloporphyrins inactivate caspase-3 and -8.

doi: 10.1096/fj.04-3259com

Figure Lengend Snippet: Figure 7. Auto-Dock results. Caspase-3 together with 10 putative binding conformations of protoporphyrin (A). Rep- resentation of the energetically most favorable caspase-3/ protoporphyrin complex showing the major protein-ligand interactions (B).

Article Snippet: Protoporphyrin IX disodium salt was from Frontier Scientific Porphyrin Products (Carnforth/ Lancashire, UK).

Techniques: Binding Assay