Journal: Translational Andrology and Urology

Article Title: Evaluation of transurethral GreenLight laser-selective vaporization for refractory overactive bladder in women

doi: 10.21037/tau-24-67

Figure Lengend Snippet: Expressions and comparisons of inflammatory factors and sensory receptors in bladder mucosa tissues before and after treatment. (A) The expressions of TRPV1, P2X3, TNF-α, and IL-6 in patients’ bladder mucosal tissues were stained by immunohistochemical staining before and at 12 weeks after treatment. Representative histological images are shown at 400× magnification. (B) Average optical density value of inflammatory factors and sensory receptors before and after treatment were presented in the bar chart. TNF, tumor necrosis factor; IL-6, interleukin 6; AOD, average optical density.

Article Snippet: Immunohistochemical staining was performed using the avidin-biotin-peroxidase method (ab64212, Abcam, Cambridge, MA, USA) with TRPV1 antibody (CSB-PA822774LA01HU, CUSABIO, Houston, TX, USA), P2X3 antibody (17843-1-AP, Proteintech, Rosemont, IL, USA), tumor necrosis factor alpha (TNF-α) antibody (17590-1-AP, Proteintech, Rosemont, IL, USA), and interleukin 6 (IL-6) antibody (21865-1-AP, Proteintech, Rosemont, IL, USA).

Techniques: Staining, Immunohistochemical staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Disruption of hippocampal P2RX2/CaMKII/NF-κB signaling contributes to learning and memory impairment in C57BL/6 mice induced by surgery plus anesthesia in neonatal period.

doi: 10.1016/j.biopha.2022.112897

Figure Lengend Snippet: Fig. 2. Surgery plus anesthesia in the neonatal period increases the levels of P2RX2, but not other purinergic receptors, in the hippocampus of juvenile mice. (A) Western blot analysis shows that surgery plus anesthesia increases the levels of P2RX2, but not P2RX1, P2RX3, P2RX4 or P2RX7 in the mouse hippocampus, as compared with the control condition. There is no statistically significant difference in the amounts of GAPDH in the hippocampus of mice following the surgery plus anesthesia or control condition. (B) Quantification of Western blot shows that surgery plus anesthesia does not alter P2RX1 levels in the mouse hippocampus as compared with the control condition. (C) Quantification of Western blot shows that surgery plus anesthesia increases P2RX2 levels in the mouse hippocampus as compared with the control condition. (D) Quantification of Western blot shows that surgery plus anesthesia does not alter P2RX3 levels in the mouse hippocampus as compared with the control condition. (E) Quantification of Western blot shows that surgery plus anesthesia does not alter P2RX4 levels in the mouse hippocampus as compared with the control condition. (F) Quantification of Western blot shows that surgery plus anesthesia does not alter P2RX7 levels in the mouse hippocampus as compared with the control condition. Data are expressed as Mean ± SD. N = 5 per arm. Student’s t-test. **P < 0.01.

Article Snippet: P2RX3 antibody (1:200 dilution; Alomone Lab) was used to recognize P2RX3 (50–75 kDa).

Techniques: Western Blot, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Disruption of hippocampal P2RX2/CaMKII/NF-κB signaling contributes to learning and memory impairment in C57BL/6 mice induced by surgery plus anesthesia in neonatal period.

doi: 10.1016/j.biopha.2022.112897

Figure Lengend Snippet: Fig. 3. Anesthesia with 3.0% sevoflurane for 2 h on PND 6 does not alter the levels of purinergic receptors P2X family in the hippocampus of juvenile mice. (A) Western blot analysis shows that sevoflurane anesthesia does not alter the levels of P2RX1, P2RX2, P2RX3, P2RX4 or P2RX7 in the mouse hippocampus, as compared with the control condition. There is no statistically significant difference in the amounts of GAPDH in the hippocampus of mice following the sevoflurane anesthesia or control condition. (B) Quantification of Western blot shows that sevoflurane anesthesia does not alter P2RX1 levels in the mouse hippocampus as compared with the control condition. (C) Quantification of Western blot shows that sevoflurane anesthesia does not alter P2RX2 levels in the mouse hippocampus as compared with the control condition. (D) Quantification of Western blot shows that sevoflurane anesthesia does not alter P2RX3 levels in the mouse hippocampus as compared with the control condition. (E) Quantification of Western blot shows that sevoflurane anesthesia does not alter P2RX4 levels in the mouse hippocampus as compared with the control condition. (F) Quantification of Western blot shows that sevoflurane anesthesia does not alter P2RX7 levels in the mouse hippocampus as compared with the control condition. Data are expressed as Mean ± SD. N = 5 per arm. Student’s t-test.

Article Snippet: P2RX3 antibody (1:200 dilution; Alomone Lab) was used to recognize P2RX3 (50–75 kDa).

Techniques: Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Adipose-Derived Stem Cells and Their Derived Microvesicles Ameliorate Detrusor Overactivity Secondary to Bilateral Partial Iliac Arterial Occlusion-Induced Bladder Ischemia

doi: 10.3390/ijms22137000

Figure Lengend Snippet: Purinergic and muscarinic signaling associated molecules changes on western blot study in separated group. showed the expression of muscarinic cholinergic (M2 and M3) and purinergic receptor proteins (P2X2 and P2X3) by Western blot. In the top panel, ( a ) showed the expression of M2 receptor is significantly decreased in BPAO + ADSCs group compared to BAPO group ( # p < 0.05). The expression of M2 receptor had trend of decreasing in BPAO + ADSC-derived MVs group compared to BAPO group without significant differences ( p > 0.05). In the middle panel, ( b ) showed that the expression of M3 receptor was significantly increased in BPAO group compared to sham group (* p < 0.05). The expression of M3 receptor is significantly decreased in BPAO + ADSC-derived MVs group compared to BAPO group ( # p < 0.05). ( c ) showed the expression of P2X2 receptors was significantly increased in BPAO group compared to sham group (* p < 0.05). The expression of P2X2 receptor is significantly decreased in BPAO + ADSC-derived MVs and BAPO + MVs group compared to BAPO group ( # p < 0.05). In the bottom panel, ( d ) showed the expression of P2X3 receptor is of no significant differences between groups.

Article Snippet: Antibodies raised against P2X2 receptors (ab10266, Cambridge, UK), P2X3 receptors (Neuromics, RA141399, Northfield, MN, USA), M2 receptors (Novus bio, nb120-2805, Novus Biologicals, LLC, Colorado, USA), M3 receptors (Abcam, ab87199, Cambridge, UK), and NGF (Abcam, ab6199, Cambridge, UK) were used.

Techniques: Western Blot, Expressing, Derivative Assay