p14 pancreas required collagenase digestion Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Millipore p14 pancreas required collagenase digestion
    Double heterozygotes have significant alterations in postnatal islet gene expression. A : expression of select endocrine development and functional genes in <t>P14</t> islets represented in a heatmap. Green, increased gene expression; red, reduced gene expression. Blue boxes, controls; green boxes, PH; red boxes, OH; black boxes, DH. B – D : relative expression levels of hormones ( B ), regulatory transcription factors ( C ), and islet functional genes ( D ) ( n = 4). One symbol, P ≤ 0.05; two symbols, P ≤ 0.01; three symbols, P ≤ 0.001. +Control (Con) vs. PH; #DH vs. PH; ^OH vs. DH; *Con vs. DH by 2-way ANOVA with Tukey’s correction for multiple comparisons or Kruskal-Wallis with Dunn’s correction for multiple comparisons.
    P14 Pancreas Required Collagenase Digestion, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p14 pancreas required collagenase digestion/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p14 pancreas required collagenase digestion - by Bioz Stars, 2021-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    Double heterozygotes have significant alterations in postnatal islet gene expression. A : expression of select endocrine development and functional genes in P14 islets represented in a heatmap. Green, increased gene expression; red, reduced gene expression. Blue boxes, controls; green boxes, PH; red boxes, OH; black boxes, DH. B – D : relative expression levels of hormones ( B ), regulatory transcription factors ( C ), and islet functional genes ( D ) ( n = 4). One symbol, P ≤ 0.05; two symbols, P ≤ 0.01; three symbols, P ≤ 0.001. +Control (Con) vs. PH; #DH vs. PH; ^OH vs. DH; *Con vs. DH by 2-way ANOVA with Tukey’s correction for multiple comparisons or Kruskal-Wallis with Dunn’s correction for multiple comparisons.

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability

    doi: 10.1152/ajpendo.00260.2017

    Figure Lengend Snippet: Double heterozygotes have significant alterations in postnatal islet gene expression. A : expression of select endocrine development and functional genes in P14 islets represented in a heatmap. Green, increased gene expression; red, reduced gene expression. Blue boxes, controls; green boxes, PH; red boxes, OH; black boxes, DH. B – D : relative expression levels of hormones ( B ), regulatory transcription factors ( C ), and islet functional genes ( D ) ( n = 4). One symbol, P ≤ 0.05; two symbols, P ≤ 0.01; three symbols, P ≤ 0.001. +Control (Con) vs. PH; #DH vs. PH; ^OH vs. DH; *Con vs. DH by 2-way ANOVA with Tukey’s correction for multiple comparisons or Kruskal-Wallis with Dunn’s correction for multiple comparisons.

    Article Snippet: Isolation of islets from P14 pancreas required collagenase digestion of whole dissected pancreas at 37°C and hand-picking of islets away from exocrine tissue following a Histopaque-1077 (Sigma-Aldrich) gradient.

    Techniques: Expressing, Functional Assay

    Islets of double heterozygotes show altered postnatal maturation. A : P14 DH islets lack a distinct β-cell core and α-cell mantle (green, insulin; red, glucagon; blue, DAPI). B – D : quantification of the percentage of islets with a mixed phenotype ( B ) and the percentage of nonmantle α-cells in each genotype at P14 ( C ), without a change in β- to α-cell ratio ( D ) ( n = 5–7). E and F : urocortin3 mRNA ( E ) and protein ( F ) are reduced in PH and DH islets ( n = 3 for mRNA, images representative of 6 animals). Scale bar: 100 μm. * P ≤ 0.05.

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability

    doi: 10.1152/ajpendo.00260.2017

    Figure Lengend Snippet: Islets of double heterozygotes show altered postnatal maturation. A : P14 DH islets lack a distinct β-cell core and α-cell mantle (green, insulin; red, glucagon; blue, DAPI). B – D : quantification of the percentage of islets with a mixed phenotype ( B ) and the percentage of nonmantle α-cells in each genotype at P14 ( C ), without a change in β- to α-cell ratio ( D ) ( n = 5–7). E and F : urocortin3 mRNA ( E ) and protein ( F ) are reduced in PH and DH islets ( n = 3 for mRNA, images representative of 6 animals). Scale bar: 100 μm. * P ≤ 0.05.

    Article Snippet: Isolation of islets from P14 pancreas required collagenase digestion of whole dissected pancreas at 37°C and hand-picking of islets away from exocrine tissue following a Histopaque-1077 (Sigma-Aldrich) gradient.

    Techniques:

    Double heterozygosity negatively impacts expression of regulatory and functional factors in postnatal islets. A : Nkx6.1 (red) is detected in fewer β-cells (insulin, green) and has reduced intensity in P14 DH. Arrowheads in DH indicate Nkx6.1 + cells. B : quantification of A ( n = 4–6). C : Glut2 expression (red) is weaker and more diffuse (E-cadherin, green) in β-cells (insulin, yellow) of DH. DAPI is in blue ( n = 4–6). Scale bar: 100 μm. **** P ≤ 0.0001 by 1-Way ANOVA with Tukey’s correction for multiple comparisons.

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability

    doi: 10.1152/ajpendo.00260.2017

    Figure Lengend Snippet: Double heterozygosity negatively impacts expression of regulatory and functional factors in postnatal islets. A : Nkx6.1 (red) is detected in fewer β-cells (insulin, green) and has reduced intensity in P14 DH. Arrowheads in DH indicate Nkx6.1 + cells. B : quantification of A ( n = 4–6). C : Glut2 expression (red) is weaker and more diffuse (E-cadherin, green) in β-cells (insulin, yellow) of DH. DAPI is in blue ( n = 4–6). Scale bar: 100 μm. **** P ≤ 0.0001 by 1-Way ANOVA with Tukey’s correction for multiple comparisons.

    Article Snippet: Isolation of islets from P14 pancreas required collagenase digestion of whole dissected pancreas at 37°C and hand-picking of islets away from exocrine tissue following a Histopaque-1077 (Sigma-Aldrich) gradient.

    Techniques: Expressing, Functional Assay