Journal: American Journal of Physiology - Endocrinology and Metabolism
Article Title: Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability
Figure Lengend Snippet: Double heterozygotes have significant alterations in postnatal islet gene expression. A : expression of select endocrine development and functional genes in P14 islets represented in a heatmap. Green, increased gene expression; red, reduced gene expression. Blue boxes, controls; green boxes, PH; red boxes, OH; black boxes, DH. B – D : relative expression levels of hormones ( B ), regulatory transcription factors ( C ), and islet functional genes ( D ) ( n = 4). One symbol, P ≤ 0.05; two symbols, P ≤ 0.01; three symbols, P ≤ 0.001. +Control (Con) vs. PH; #DH vs. PH; ^OH vs. DH; *Con vs. DH by 2-way ANOVA with Tukey’s correction for multiple comparisons or Kruskal-Wallis with Dunn’s correction for multiple comparisons.
Article Snippet: Isolation of islets from P14 pancreas required collagenase digestion of whole dissected pancreas at 37°C and hand-picking of islets away from exocrine tissue following a Histopaque-1077 (Sigma-Aldrich) gradient.
Techniques: Expressing, Functional Assay