p130 cas sirna Search Results


p130cas sirna sc 36142  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology p130cas sirna sc 36142
Downstream targets of Src in mouse and cellular models of SBMA. a – c Immunoblots showing the levels of phosphorylated and total <t>p130Cas,</t> Stat3, p38MAPK, and Akt proteins in the spinal cord and skeletal muscle of 9-week-old AR-97Q mice treated with or without A419259 ( a ). Quantitative analyses were performed using densitometry ( b , c ). d – f Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, p38MAPK, and Akt proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q with or without 0.2 µM of A419259 administration ( d ). Quantitative analyses were performed using densitometry ( e , f ). g – i Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, and p38MAPK proteins in the NSC34 and C2C12 cells transfected with mock or Src plasmids ( g ). Quantitative analyses were performed using densitometry ( h , i ). Quantitative analyses were performed on samples from n = 3 animals per group. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f , h , i ). * p < 0.05 and ** p < 0.01. N.S., not significant. Source data are provided as a Source Data file ( a – i )
P130cas Sirna Sc 36142, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p130cas sirna sc 36142/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
p130cas sirna sc 36142 - by Bioz Stars, 2024-12
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p130 cas individual sirnas  (Santa Cruz Biotechnology)
86
Santa Cruz Biotechnology p130 cas individual sirnas
Downstream targets of Src in mouse and cellular models of SBMA. a – c Immunoblots showing the levels of phosphorylated and total <t>p130Cas,</t> Stat3, p38MAPK, and Akt proteins in the spinal cord and skeletal muscle of 9-week-old AR-97Q mice treated with or without A419259 ( a ). Quantitative analyses were performed using densitometry ( b , c ). d – f Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, p38MAPK, and Akt proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q with or without 0.2 µM of A419259 administration ( d ). Quantitative analyses were performed using densitometry ( e , f ). g – i Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, and p38MAPK proteins in the NSC34 and C2C12 cells transfected with mock or Src plasmids ( g ). Quantitative analyses were performed using densitometry ( h , i ). Quantitative analyses were performed on samples from n = 3 animals per group. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f , h , i ). * p < 0.05 and ** p < 0.01. N.S., not significant. Source data are provided as a Source Data file ( a – i )
P130 Cas Individual Sirnas, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p130 cas individual sirnas/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
p130 cas individual sirnas - by Bioz Stars, 2024-12
86/100 stars
  Buy from Supplier

Image Search Results


Downstream targets of Src in mouse and cellular models of SBMA. a – c Immunoblots showing the levels of phosphorylated and total p130Cas, Stat3, p38MAPK, and Akt proteins in the spinal cord and skeletal muscle of 9-week-old AR-97Q mice treated with or without A419259 ( a ). Quantitative analyses were performed using densitometry ( b , c ). d – f Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, p38MAPK, and Akt proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q with or without 0.2 µM of A419259 administration ( d ). Quantitative analyses were performed using densitometry ( e , f ). g – i Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, and p38MAPK proteins in the NSC34 and C2C12 cells transfected with mock or Src plasmids ( g ). Quantitative analyses were performed using densitometry ( h , i ). Quantitative analyses were performed on samples from n = 3 animals per group. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f , h , i ). * p < 0.05 and ** p < 0.01. N.S., not significant. Source data are provided as a Source Data file ( a – i )

Journal: Nature Communications

Article Title: Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy

doi: 10.1038/s41467-019-12282-7

Figure Lengend Snippet: Downstream targets of Src in mouse and cellular models of SBMA. a – c Immunoblots showing the levels of phosphorylated and total p130Cas, Stat3, p38MAPK, and Akt proteins in the spinal cord and skeletal muscle of 9-week-old AR-97Q mice treated with or without A419259 ( a ). Quantitative analyses were performed using densitometry ( b , c ). d – f Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, p38MAPK, and Akt proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q with or without 0.2 µM of A419259 administration ( d ). Quantitative analyses were performed using densitometry ( e , f ). g – i Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, and p38MAPK proteins in the NSC34 and C2C12 cells transfected with mock or Src plasmids ( g ). Quantitative analyses were performed using densitometry ( h , i ). Quantitative analyses were performed on samples from n = 3 animals per group. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f , h , i ). * p < 0.05 and ** p < 0.01. N.S., not significant. Source data are provided as a Source Data file ( a – i )

Article Snippet: Another siRNA to knockdown p130Cas (p130Cas siRNA-2) were p130Cas siRNA (sc-36142) (Santa Cruz Biotechnology, Texas, USA).

Techniques: Western Blot, Stable Transfection, Expressing, Transfection

p130Cas is activated in cellular and mouse models of SBMA. a , b Immunoblots showing the levels of the phosphorylated and total p130Cas proteins in NSC34 cells and C2C12 cells stably expressing AR-24Q or AR-97Q that were treated with or without DHT ( a ). Quantitative analyses were performed using densitometry ( n = 3) ( b ). c – e Immunoblots showing levels of the p-p130Cas and p130Cas proteins in the spinal cord and skeletal muscle of wild-type and AR-97Q mice at 6, 9 and 13 weeks of age ( c ). Quantitative analyses were performed using densitometry ( n = 3) ( d , e ). f , g Immunohistochemistry for p-p130Cas in spinal cord ( f ) and skeletal muscle ( g ) samples from 6-week-old mice. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , d , e ). * p < 0.05 and ** p < 0.01. Wt, wild-type; AR, AR-97Q; N.S., not significant. Scale bars: 25 µm ( f, g ). Source data are provided as a Source Data file ( a – e )

Journal: Nature Communications

Article Title: Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy

doi: 10.1038/s41467-019-12282-7

Figure Lengend Snippet: p130Cas is activated in cellular and mouse models of SBMA. a , b Immunoblots showing the levels of the phosphorylated and total p130Cas proteins in NSC34 cells and C2C12 cells stably expressing AR-24Q or AR-97Q that were treated with or without DHT ( a ). Quantitative analyses were performed using densitometry ( n = 3) ( b ). c – e Immunoblots showing levels of the p-p130Cas and p130Cas proteins in the spinal cord and skeletal muscle of wild-type and AR-97Q mice at 6, 9 and 13 weeks of age ( c ). Quantitative analyses were performed using densitometry ( n = 3) ( d , e ). f , g Immunohistochemistry for p-p130Cas in spinal cord ( f ) and skeletal muscle ( g ) samples from 6-week-old mice. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , d , e ). * p < 0.05 and ** p < 0.01. Wt, wild-type; AR, AR-97Q; N.S., not significant. Scale bars: 25 µm ( f, g ). Source data are provided as a Source Data file ( a – e )

Article Snippet: Another siRNA to knockdown p130Cas (p130Cas siRNA-2) were p130Cas siRNA (sc-36142) (Santa Cruz Biotechnology, Texas, USA).

Techniques: Western Blot, Stable Transfection, Expressing, Immunohistochemistry

Phosphorylation of p130Cas exerts toxic effects on cellular models of SBMA. a Immunoblots showing the levels of the p-p130Cas and p130Cas proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q and transfected with the mock or p130Cas siRNA-1. b , c The viability, cell numbers and LDH release from NSC34 ( b ) and C2C12 cells ( c ) are shown ( n = 3). d Immunoblots showing the levels of phosphorylated and total p130Cas proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q and transfected with the mock or p130Cas vector. e , f Viability, cell numbers and LDH release from NSC34 ( e ) and C2C12 ( f ) cells are shown ( n = 3). Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f ). * p < 0.05 and ** p < 0.01. Source data are provided as a Source Data file ( a – f )

Journal: Nature Communications

Article Title: Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy

doi: 10.1038/s41467-019-12282-7

Figure Lengend Snippet: Phosphorylation of p130Cas exerts toxic effects on cellular models of SBMA. a Immunoblots showing the levels of the p-p130Cas and p130Cas proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q and transfected with the mock or p130Cas siRNA-1. b , c The viability, cell numbers and LDH release from NSC34 ( b ) and C2C12 cells ( c ) are shown ( n = 3). d Immunoblots showing the levels of phosphorylated and total p130Cas proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q and transfected with the mock or p130Cas vector. e , f Viability, cell numbers and LDH release from NSC34 ( e ) and C2C12 ( f ) cells are shown ( n = 3). Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f ). * p < 0.05 and ** p < 0.01. Source data are provided as a Source Data file ( a – f )

Article Snippet: Another siRNA to knockdown p130Cas (p130Cas siRNA-2) were p130Cas siRNA (sc-36142) (Santa Cruz Biotechnology, Texas, USA).

Techniques: Western Blot, Stable Transfection, Expressing, Transfection, Plasmid Preparation