p0704 Search Results


non reducing format rapid pngasef  (New England Biolabs)
98
New England Biolabs non reducing format rapid pngasef
Predominant N-glycosylation and limited O-glycosylation in HeV-G. (A) NetNGlyc 1.0 prediction of N-glycosylation sites in HeV-G sequence, which applies artificial neural networks to evaluate the sequence context of Asn-Xaa-Ser/Thr sequons. ( B ) SDS-PAGE analysis of HeV-G <t>under</t> <t>non-reducing</t> conditions following treatment with the indicated glycosidases. ( C ) Periodic acid–Schiff (PAS) staining of treated HeV-G samples following deglycosylation. Lane 1: Marker; Lane 2: 5 µg Untreated HeV-G; Lane 3: 5 µg HeV-G + PNGase F; Lane 4: 5 µg HeV-G + Rapid PNGase F; Lane 5: 5 µg HeV-G + O-Glycosidase (OG) and Neuraminidase (NA); Lane 6: 5 µg HeV-G + Deglycosylation Mix Ⅱ; Lane 7: Horseradish Peroxidase (HRP, positive control); Lane 8: Soybean Trypsin Inhibitor (STI, negative control). The distinct bands correspond to the HeV-G monomer, dimer and tetramer, while the band below 40 kDa represents PNGase F
Non Reducing Format Rapid Pngasef, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/non reducing format rapid pngasef/product/New England Biolabs
Average 98 stars, based on 1 article reviews
non reducing format rapid pngasef - by Bioz Stars, 2026-04
98/100 stars
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wts-lv in situ pump p0704  (McLane Research Laboratories)
90
McLane Research Laboratories wts-lv in situ pump p0704
Predominant N-glycosylation and limited O-glycosylation in HeV-G. (A) NetNGlyc 1.0 prediction of N-glycosylation sites in HeV-G sequence, which applies artificial neural networks to evaluate the sequence context of Asn-Xaa-Ser/Thr sequons. ( B ) SDS-PAGE analysis of HeV-G <t>under</t> <t>non-reducing</t> conditions following treatment with the indicated glycosidases. ( C ) Periodic acid–Schiff (PAS) staining of treated HeV-G samples following deglycosylation. Lane 1: Marker; Lane 2: 5 µg Untreated HeV-G; Lane 3: 5 µg HeV-G + PNGase F; Lane 4: 5 µg HeV-G + Rapid PNGase F; Lane 5: 5 µg HeV-G + O-Glycosidase (OG) and Neuraminidase (NA); Lane 6: 5 µg HeV-G + Deglycosylation Mix Ⅱ; Lane 7: Horseradish Peroxidase (HRP, positive control); Lane 8: Soybean Trypsin Inhibitor (STI, negative control). The distinct bands correspond to the HeV-G monomer, dimer and tetramer, while the band below 40 kDa represents PNGase F
Wts Lv In Situ Pump P0704, supplied by McLane Research Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wts-lv in situ pump p0704/product/McLane Research Laboratories
Average 90 stars, based on 1 article reviews
wts-lv in situ pump p0704 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Predominant N-glycosylation and limited O-glycosylation in HeV-G. (A) NetNGlyc 1.0 prediction of N-glycosylation sites in HeV-G sequence, which applies artificial neural networks to evaluate the sequence context of Asn-Xaa-Ser/Thr sequons. ( B ) SDS-PAGE analysis of HeV-G under non-reducing conditions following treatment with the indicated glycosidases. ( C ) Periodic acid–Schiff (PAS) staining of treated HeV-G samples following deglycosylation. Lane 1: Marker; Lane 2: 5 µg Untreated HeV-G; Lane 3: 5 µg HeV-G + PNGase F; Lane 4: 5 µg HeV-G + Rapid PNGase F; Lane 5: 5 µg HeV-G + O-Glycosidase (OG) and Neuraminidase (NA); Lane 6: 5 µg HeV-G + Deglycosylation Mix Ⅱ; Lane 7: Horseradish Peroxidase (HRP, positive control); Lane 8: Soybean Trypsin Inhibitor (STI, negative control). The distinct bands correspond to the HeV-G monomer, dimer and tetramer, while the band below 40 kDa represents PNGase F

Journal: Virology Journal

Article Title: Characterization and functional analysis of N-linked glycosylation on the Hendra virus attachment glycoprotein

doi: 10.1186/s12985-026-03095-4

Figure Lengend Snippet: Predominant N-glycosylation and limited O-glycosylation in HeV-G. (A) NetNGlyc 1.0 prediction of N-glycosylation sites in HeV-G sequence, which applies artificial neural networks to evaluate the sequence context of Asn-Xaa-Ser/Thr sequons. ( B ) SDS-PAGE analysis of HeV-G under non-reducing conditions following treatment with the indicated glycosidases. ( C ) Periodic acid–Schiff (PAS) staining of treated HeV-G samples following deglycosylation. Lane 1: Marker; Lane 2: 5 µg Untreated HeV-G; Lane 3: 5 µg HeV-G + PNGase F; Lane 4: 5 µg HeV-G + Rapid PNGase F; Lane 5: 5 µg HeV-G + O-Glycosidase (OG) and Neuraminidase (NA); Lane 6: 5 µg HeV-G + Deglycosylation Mix Ⅱ; Lane 7: Horseradish Peroxidase (HRP, positive control); Lane 8: Soybean Trypsin Inhibitor (STI, negative control). The distinct bands correspond to the HeV-G monomer, dimer and tetramer, while the band below 40 kDa represents PNGase F

Article Snippet: For rapid N-glycan removal, a separate aliquot of glycoprotein (10 μg) was incubated with non-reducing format Rapid PNGaseF (New England Biolabs, 1x concentration) at 50 °C for 10 min according to the manufacturer’s protocol.

Techniques: Glycoproteomics, Sequencing, SDS Page, Staining, Marker, Positive Control, Negative Control