Journal: Apoptosis : an international journal on programmed cell death

Article Title: TNF-α and RPLP0 drive the apoptosis of endothelial cells and increase susceptibility to high-altitude pulmonary edema.

doi: 10.1007/s10495-024-02005-9

Figure Lengend Snippet: Fig. 8 Validation of TNF-α upregulation and RPLP0 downregulation in HAPE rats. A, Expression levels of TNF and RPLP0 in GSE52209. B and C, Rats were subjected to a simulated altitude of 6000 m for 3 days in a hypobaric chamber. The HAPE rat model was verified by assessing the lung wet-to-dry weight ratio (B) and histological changes in the lungs using hematoxylin and eosin (HE) staining (C) (n = 12 for each group; scale bars: 50 μm). D, Relative mRNA expression level of Tnf-α in the lung tissue of HAPE rats, as determined by RT‒qPCR analysis (n = 12 for each group). E and F, Protein levels of TNF-α in the lung tissue (E) and serum (F) of HAPE rats were measured using ELISA (n = 12 for each group). G, Relative mRNA expression level of Rplp0 in the lung tissue of HAPE rats, as determined by RT‒qPCR analysis (n = 12 for each group). H, Relative protein level of RPLP0 in the lung tissue of HAPE rats, as determined by western blotting analy sis (n = 8 for each group). Continuous variables with a normal distribu tion are presented as means ± SEM. Significance was determined by ordinary one-way ANOVA followed by Tukey’s multiple comparisons (A) and 2-tailed unpaired Student’s t test with Welch’s correction (B and D-H). *P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001 ver sus the control

Article Snippet: The membrane was blocked with 5% BSA in Tris-buffered saline with 0.1% Tween 20, followed by incubation with primary antibodies against cleaved caspase-3 at a 1:2000 dilution (#9661; Cell Signaling Technology, USA) and RPLP0 at a 1:1000 dilution (11290-2-AP; Proteintech, China) overnight at 4 °C.

Techniques: Biomarker Discovery, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: PLoS ONE

Article Title: Epitope Analysis of the Collagen Type V-Specific T Cell Response in Lung Transplantation Reveals an HLA-DRB1*15 Bias in Both Recipient and Donor

doi: 10.1371/journal.pone.0079601

Figure Lengend Snippet: Univariate (chi square) and Multivariate analysis of associations with Col(V) reactivity >20x10 -4 inches in TV-DTH in end-stage lung disease and prevalence of HLA-DR15.

Article Snippet: Univariate analysis of common HLA class II types in this patient cohort with lung disease also revealed that subjects with HLA-DR15 ( , red symbols) were more highly represented in the col(V)-responsive group (53% DR15 + ) versus non-responders (28% DR15 + ; Chi Square p=0.01, ).

Techniques: