p-80 Search Results


tnfr2 antibody  (Proteintech)
95
Proteintech tnfr2 antibody
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
Tnfr2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/TNFR2+Antibody/pmc10771422-380-5-47
Average 95 stars, based on 1 article reviews
tnfr2 antibody - by Bioz Stars, 2026-09
95/100 stars
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rabbit anti katnb1  (Proteintech)
93
Proteintech rabbit anti katnb1
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
Rabbit Anti Katnb1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/KATNB1+Antibody/pmc08240857-263-39-42
Average 93 stars, based on 1 article reviews
rabbit anti katnb1 - by Bioz Stars, 2026-09
93/100 stars
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anti il 1r1  (Proteintech)
94
Proteintech anti il 1r1
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
Anti Il 1r1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/Human+IL-1R1+ELISA+Kit/pmc11920578-80-31-33
Average 94 stars, based on 1 article reviews
anti il 1r1 - by Bioz Stars, 2026-09
94/100 stars
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p 80 aeruginosa  (ATCC)
94
ATCC p 80 aeruginosa
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
P 80 Aeruginosa, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/p80/10__1128_slash_aac__00212___20-20-7-26
Average 94 stars, based on 1 article reviews
p 80 aeruginosa - by Bioz Stars, 2026-09
94/100 stars
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human igg isotype control antibody  (Bethyl)
93
Bethyl human igg isotype control antibody
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
Human Igg Isotype Control Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/Purified+Human+IgG/pmc05502430-188-30-35
Average 93 stars, based on 1 article reviews
human igg isotype control antibody - by Bioz Stars, 2026-09
93/100 stars
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human igg kappa  (Bethyl)
93
Bethyl human igg kappa
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
Human Igg Kappa, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/Purified+Human+IgG%2FKappa/pmc04030719-225-25-27
Average 93 stars, based on 1 article reviews
human igg kappa - by Bioz Stars, 2026-09
93/100 stars
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fab standard  (Bethyl)
91
Bethyl fab standard
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
Fab Standard, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/Purified+Human+Fab%2FKappa/pm36057427-92-19-26
Average 91 stars, based on 1 article reviews
fab standard - by Bioz Stars, 2026-09
91/100 stars
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coilin  (Proteintech)
93
Proteintech coilin
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
Coilin, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/Coilin+Antibody/pm41456942-110-48-50
Average 93 stars, based on 1 article reviews
coilin - by Bioz Stars, 2026-09
93/100 stars
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p80  (Bethyl)
93
Bethyl p80
A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, <t>TNFR2,</t> TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.
P80, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/Purified+Human+IgA/pm38667159-46-9-13
Average 93 stars, based on 1 article reviews
p80 - by Bioz Stars, 2026-09
93/100 stars
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ercc2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ercc2
CIA components and substrates form higher order complexes . A , HEK293 cells were treated with 100 μg/ml ammonium ferric citrate (FAC) or 100 μM deferoxamine (DFO) for 8 h to create an iron-sufficient or -deficient environment, respectively. Whole cell extracts (WCEs) were resolved by SDS-PAGE and blotted with antibodies against known components of the cytosolic iron–sulfur cluster assembly (CIA) pathway, CIA substrates, loading control α-tubulin, FAC treatment control FBXL5, and DFO treatment control IREB2. B , Flp-In T-Rex 293 cell line (Flp-In 293) engineered to stably express NUBP2 was induced overnight with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by bottom-up proteomics. Data-dependent acquisition of two technical replicates was performed. Spectra count (SpC) for selected proteins combined from the two technical replicates was shown. C , parallel reaction monitoring was conducted on HEK293 whole cell lysate with indicated amounts of protein using a targeted proteomics assay that monitors the abundance of known CIA factors (ABCB7, GLRX3, BOLA2, CIAPIN1, NUBP1, NUBP2, CIAO3, CIAO1, CIAO2B, CIAO2A, MMS19) and prototypical substrates (CDKAL1, DNA2, <t>ERCC2,</t> POLD1, and ABCE1). Two technical replicates were acquired. Intensities were normalized for each precursor to the highest intensity in a replicate. D , Flp-In 293 cells that stably express NUBP2 were induced with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by acquisition of a targeted proteomic assay containing CIA scaffold complex components (NUBP1 and NUBP2), CIAO3, CIA targeting complex components (MMS19, CIAO1, and CIAO2B) and prototypical substrates (CDKAL1, DNA2, ERCC2, POLD1, and ABCE1). Two biological replicates were performed. Acquired spectra were searched with MaxQuant. Each edge represents a peptide identified. Solid edges connect bait protein to known interactors while dashed edges connect to novel interactors discovered in our study. Edge widths correspond to the posterior error probability of each peptide. E , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA immunoprecipitates were blotted for CIAO3, CIA targeting complex components, and the CIA substrate CDKAL1. CIAO1: probable cytosolic iron-sulfur protein assembly protein CIAO1; CIAO2B: cytosolic iron-sulfur assembly component 2B; CIAO3: cytosolic iron-sulfur assembly component 3; MMS19: MMS19 nucleotide excision repair protein homolog; NUBP1: nucleotide-binding protein 1; NUBP2: nucleotide-binding protein 2.
Ercc2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/TFIIH+p80+Antibody/pmc09243173-194-66-67
Average 93 stars, based on 1 article reviews
ercc2 - by Bioz Stars, 2026-09
93/100 stars
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anti uaf1  (Proteintech)
94
Proteintech anti uaf1
CIA components and substrates form higher order complexes . A , HEK293 cells were treated with 100 μg/ml ammonium ferric citrate (FAC) or 100 μM deferoxamine (DFO) for 8 h to create an iron-sufficient or -deficient environment, respectively. Whole cell extracts (WCEs) were resolved by SDS-PAGE and blotted with antibodies against known components of the cytosolic iron–sulfur cluster assembly (CIA) pathway, CIA substrates, loading control α-tubulin, FAC treatment control FBXL5, and DFO treatment control IREB2. B , Flp-In T-Rex 293 cell line (Flp-In 293) engineered to stably express NUBP2 was induced overnight with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by bottom-up proteomics. Data-dependent acquisition of two technical replicates was performed. Spectra count (SpC) for selected proteins combined from the two technical replicates was shown. C , parallel reaction monitoring was conducted on HEK293 whole cell lysate with indicated amounts of protein using a targeted proteomics assay that monitors the abundance of known CIA factors (ABCB7, GLRX3, BOLA2, CIAPIN1, NUBP1, NUBP2, CIAO3, CIAO1, CIAO2B, CIAO2A, MMS19) and prototypical substrates (CDKAL1, DNA2, <t>ERCC2,</t> POLD1, and ABCE1). Two technical replicates were acquired. Intensities were normalized for each precursor to the highest intensity in a replicate. D , Flp-In 293 cells that stably express NUBP2 were induced with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by acquisition of a targeted proteomic assay containing CIA scaffold complex components (NUBP1 and NUBP2), CIAO3, CIA targeting complex components (MMS19, CIAO1, and CIAO2B) and prototypical substrates (CDKAL1, DNA2, ERCC2, POLD1, and ABCE1). Two biological replicates were performed. Acquired spectra were searched with MaxQuant. Each edge represents a peptide identified. Solid edges connect bait protein to known interactors while dashed edges connect to novel interactors discovered in our study. Edge widths correspond to the posterior error probability of each peptide. E , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA immunoprecipitates were blotted for CIAO3, CIA targeting complex components, and the CIA substrate CDKAL1. CIAO1: probable cytosolic iron-sulfur protein assembly protein CIAO1; CIAO2B: cytosolic iron-sulfur assembly component 2B; CIAO3: cytosolic iron-sulfur assembly component 3; MMS19: MMS19 nucleotide excision repair protein homolog; NUBP1: nucleotide-binding protein 1; NUBP2: nucleotide-binding protein 2.
Anti Uaf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/WDR48+Antibody/pm32133736-372-20-19
Average 94 stars, based on 1 article reviews
anti uaf1 - by Bioz Stars, 2026-09
94/100 stars
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anti ercc2  (Proteintech)
93
Proteintech anti ercc2
CIA components and substrates form higher order complexes . A , HEK293 cells were treated with 100 μg/ml ammonium ferric citrate (FAC) or 100 μM deferoxamine (DFO) for 8 h to create an iron-sufficient or -deficient environment, respectively. Whole cell extracts (WCEs) were resolved by SDS-PAGE and blotted with antibodies against known components of the cytosolic iron–sulfur cluster assembly (CIA) pathway, CIA substrates, loading control α-tubulin, FAC treatment control FBXL5, and DFO treatment control IREB2. B , Flp-In T-Rex 293 cell line (Flp-In 293) engineered to stably express NUBP2 was induced overnight with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by bottom-up proteomics. Data-dependent acquisition of two technical replicates was performed. Spectra count (SpC) for selected proteins combined from the two technical replicates was shown. C , parallel reaction monitoring was conducted on HEK293 whole cell lysate with indicated amounts of protein using a targeted proteomics assay that monitors the abundance of known CIA factors (ABCB7, GLRX3, BOLA2, CIAPIN1, NUBP1, NUBP2, CIAO3, CIAO1, CIAO2B, CIAO2A, MMS19) and prototypical substrates (CDKAL1, DNA2, <t>ERCC2,</t> POLD1, and ABCE1). Two technical replicates were acquired. Intensities were normalized for each precursor to the highest intensity in a replicate. D , Flp-In 293 cells that stably express NUBP2 were induced with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by acquisition of a targeted proteomic assay containing CIA scaffold complex components (NUBP1 and NUBP2), CIAO3, CIA targeting complex components (MMS19, CIAO1, and CIAO2B) and prototypical substrates (CDKAL1, DNA2, ERCC2, POLD1, and ABCE1). Two biological replicates were performed. Acquired spectra were searched with MaxQuant. Each edge represents a peptide identified. Solid edges connect bait protein to known interactors while dashed edges connect to novel interactors discovered in our study. Edge widths correspond to the posterior error probability of each peptide. E , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA immunoprecipitates were blotted for CIAO3, CIA targeting complex components, and the CIA substrate CDKAL1. CIAO1: probable cytosolic iron-sulfur protein assembly protein CIAO1; CIAO2B: cytosolic iron-sulfur assembly component 2B; CIAO3: cytosolic iron-sulfur assembly component 3; MMS19: MMS19 nucleotide excision repair protein homolog; NUBP1: nucleotide-binding protein 1; NUBP2: nucleotide-binding protein 2.
Anti Ercc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-80/ERCC2+Antibody/pmc09893563-283-24-26
Average 93 stars, based on 1 article reviews
anti ercc2 - by Bioz Stars, 2026-09
93/100 stars
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A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, TNFR2, TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.

Journal: Nature Communications

Article Title: Targeted therapies of inflammatory diseases with intracellularly gelated macrophages in mice and rats

doi: 10.1038/s41467-023-44662-5

Figure Lengend Snippet: A Membrane protein bands of MAs and GMs via Coomassie blue staining. B Membrane protein contents of MAs and GMs determined by BCA assay. C Western blot analysis on the expression of IL-6Rα, IL-6Rβ, TNFR2, TNFR1 and IL-1R2 in the members of MAs and GMs, respectively. D Quantitative result of protein expression. E Different doses of GMs (0.15, 0.3, 0.6, 1.2, 2.4, 4.8 and 9.6 × 10 6 mL −1 ) were incubated with PBS solution containing 10 μg mL −1 each of TNF-α and IL-1β, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits after incubation for 1 h. F MAs were co-treated with 100 ng mL −1 of LPS and different dose of GMs (0.1875, 0.375, 0.75, 1.5 and 3, ×10 6 mL −1 ) for 12 h, and the supernatant concentrations of TNF-α and IL-1β were measured by Elisa kits. G Schematic illustration of IL-6 trans-signalling pathway. H MAs, GMs and PFA-MAs were incubated with 10 ng mL −1 of IL-6 for 12 h, and incubated with rabbit gp130 primary antibody and mouse IL-6R primary antibody, respectively, followed by incubation with anti-rabbit IgG Fab2 Alexa Fluor 488 and anti-mouse IgG Fab2 Alexa Fluor 594, and fluorescence imaging was conducted by confocal microscopy. Scale bar: 5 μm. I Pearson’s correlation coefficient of fluorescence co-localization was determined by ImageJ. J MAs, GMs and PFA-MAs at the number of 3 × 10 6 mL −1 were incubated with 10 μg mL -1 of IL-6 for 12 h, and the remaining IL-6 concentration was determined by Elisa kit. All data was presented as mean ± s.d. ( n = 3). Representative photos in ( A , C – H ) came from three independent experiments on three batches of MAs and GMs ( n = 3). All statistical analyses were conducted using One-Way ANOVA.

Article Snippet: TNFR1 antibody (Cat. No. 21574-1-AP), TNFR2 antibody (Cat. No. 19272-1-AP), IL-6Rα antibody (Cat. No. 23457-1-AP), IL-6Rβ antibody (Cat. No. 67766-1-Ig), IL-1R2 antibody (Cat. No. 60262-1-Ig), gp130 antibody (21175-1-AP), CD248 antibody (Cat. No. 60170-1-1 g), and fibronectin antibody (Cat. No. 66042-1-1 g) (Supplementary Table ) were purchased from Proteintech (China).

Techniques: Membrane, Staining, BIA-KA, Western Blot, Expressing, Incubation, Enzyme-linked Immunosorbent Assay, Fluorescence, Imaging, Confocal Microscopy, Concentration Assay

CIA components and substrates form higher order complexes . A , HEK293 cells were treated with 100 μg/ml ammonium ferric citrate (FAC) or 100 μM deferoxamine (DFO) for 8 h to create an iron-sufficient or -deficient environment, respectively. Whole cell extracts (WCEs) were resolved by SDS-PAGE and blotted with antibodies against known components of the cytosolic iron–sulfur cluster assembly (CIA) pathway, CIA substrates, loading control α-tubulin, FAC treatment control FBXL5, and DFO treatment control IREB2. B , Flp-In T-Rex 293 cell line (Flp-In 293) engineered to stably express NUBP2 was induced overnight with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by bottom-up proteomics. Data-dependent acquisition of two technical replicates was performed. Spectra count (SpC) for selected proteins combined from the two technical replicates was shown. C , parallel reaction monitoring was conducted on HEK293 whole cell lysate with indicated amounts of protein using a targeted proteomics assay that monitors the abundance of known CIA factors (ABCB7, GLRX3, BOLA2, CIAPIN1, NUBP1, NUBP2, CIAO3, CIAO1, CIAO2B, CIAO2A, MMS19) and prototypical substrates (CDKAL1, DNA2, ERCC2, POLD1, and ABCE1). Two technical replicates were acquired. Intensities were normalized for each precursor to the highest intensity in a replicate. D , Flp-In 293 cells that stably express NUBP2 were induced with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by acquisition of a targeted proteomic assay containing CIA scaffold complex components (NUBP1 and NUBP2), CIAO3, CIA targeting complex components (MMS19, CIAO1, and CIAO2B) and prototypical substrates (CDKAL1, DNA2, ERCC2, POLD1, and ABCE1). Two biological replicates were performed. Acquired spectra were searched with MaxQuant. Each edge represents a peptide identified. Solid edges connect bait protein to known interactors while dashed edges connect to novel interactors discovered in our study. Edge widths correspond to the posterior error probability of each peptide. E , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA immunoprecipitates were blotted for CIAO3, CIA targeting complex components, and the CIA substrate CDKAL1. CIAO1: probable cytosolic iron-sulfur protein assembly protein CIAO1; CIAO2B: cytosolic iron-sulfur assembly component 2B; CIAO3: cytosolic iron-sulfur assembly component 3; MMS19: MMS19 nucleotide excision repair protein homolog; NUBP1: nucleotide-binding protein 1; NUBP2: nucleotide-binding protein 2.

Journal: The Journal of Biological Chemistry

Article Title: Iron-regulated assembly of the cytosolic iron–sulfur cluster biogenesis machinery

doi: 10.1016/j.jbc.2022.102094

Figure Lengend Snippet: CIA components and substrates form higher order complexes . A , HEK293 cells were treated with 100 μg/ml ammonium ferric citrate (FAC) or 100 μM deferoxamine (DFO) for 8 h to create an iron-sufficient or -deficient environment, respectively. Whole cell extracts (WCEs) were resolved by SDS-PAGE and blotted with antibodies against known components of the cytosolic iron–sulfur cluster assembly (CIA) pathway, CIA substrates, loading control α-tubulin, FAC treatment control FBXL5, and DFO treatment control IREB2. B , Flp-In T-Rex 293 cell line (Flp-In 293) engineered to stably express NUBP2 was induced overnight with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by bottom-up proteomics. Data-dependent acquisition of two technical replicates was performed. Spectra count (SpC) for selected proteins combined from the two technical replicates was shown. C , parallel reaction monitoring was conducted on HEK293 whole cell lysate with indicated amounts of protein using a targeted proteomics assay that monitors the abundance of known CIA factors (ABCB7, GLRX3, BOLA2, CIAPIN1, NUBP1, NUBP2, CIAO3, CIAO1, CIAO2B, CIAO2A, MMS19) and prototypical substrates (CDKAL1, DNA2, ERCC2, POLD1, and ABCE1). Two technical replicates were acquired. Intensities were normalized for each precursor to the highest intensity in a replicate. D , Flp-In 293 cells that stably express NUBP2 were induced with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by acquisition of a targeted proteomic assay containing CIA scaffold complex components (NUBP1 and NUBP2), CIAO3, CIA targeting complex components (MMS19, CIAO1, and CIAO2B) and prototypical substrates (CDKAL1, DNA2, ERCC2, POLD1, and ABCE1). Two biological replicates were performed. Acquired spectra were searched with MaxQuant. Each edge represents a peptide identified. Solid edges connect bait protein to known interactors while dashed edges connect to novel interactors discovered in our study. Edge widths correspond to the posterior error probability of each peptide. E , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA immunoprecipitates were blotted for CIAO3, CIA targeting complex components, and the CIA substrate CDKAL1. CIAO1: probable cytosolic iron-sulfur protein assembly protein CIAO1; CIAO2B: cytosolic iron-sulfur assembly component 2B; CIAO3: cytosolic iron-sulfur assembly component 3; MMS19: MMS19 nucleotide excision repair protein homolog; NUBP1: nucleotide-binding protein 1; NUBP2: nucleotide-binding protein 2.

Article Snippet: Membranes were blocked with either 5% milk or 5% BSA before blotting with primary antibodies against CIAPIN1 (Santa Cruz Biotechnology sc-271298, Lot #H2317), GLRX3 (Santa Cruz Biotechnology sc-100601 Lot#C1811), CIAO3 (Santa Cruz Biotechnology sc-514078 Lot #K1914 or Sigma Aldrich SAB4502760), NUBP1 (Santa Cruz Biotechnology sc-514175, Lot #0715), NUBP2 (Proteintech 15409-1-AP), MMS19 (Proteintech 16015-1-AP or 66049-1-IG), CIAO1 (Cell Signaling 87027S Lot:1), CIAO2B (Proteintech 20108-1-AP), CIAO2A (Proteintech 20776-1-AP), ERCC2 (Santa Cruz Biotechnology sc-101174, Lot #K0414), FTH1 (Cell Signaling #3998), FLAG (Sigma #F1804 and Proteintech), ISCU1/2 (Proteintech), HIF1α (Bethyl A300–286A), IRP2 (Santa Cruz Biotechnology sc-33682, Lot #B1116), FBXL5 (BioLegend Clone 3F5G12G9 or 10F4H9D12), GAPDH (Proteintech HRP-60004), and TUBA (Proteintech).

Techniques: SDS Page, Control, Stable Transfection, Affinity Purification, Data-dependent acquisition, Targeted Proteomics, Proteomic Assay, Expressing, Immunoprecipitation, Binding Assay

Fe-S cluster incorporation into CIAO3 controls its interactions. A , schematic representation of Fe-S incorporation in wildtype CIAO3 and mutants. B – D , Flp-In 293 cells that stably express wildtype or mutant CIAO3 were induced with 1 μg/ml doxycycline for 24 h before harvesting. CIAO3 and associated proteins were affinity purified. Two biological replicates were analyzed. Protein abundances were monitored by a targeted proteomic assay containing known CIA components and a subset of prototypical CIA substrates after normalization to CIAO3 levels. Log2 transformed abundance of the co-eluted proteins (Log2Intensiteis) were plotted for the CIA scaffold complex ( B ), the CIA targeting complex ( C ), and selected CIA substrates ( D ). Mean ± SD was indicated. Protein levels of interactors co-eluted with mutant CIAO3 were compared to wildtype. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001. E , Flp-In 293 control cells, cells expressing wildtype 3HA-3FLAG-ERCC2 and cells expressing mutant ERCC2 lacking the MMS19 binding region were induced with 1 μg/ml doxycycline for 24 h. Cell lysates were immunoprecipitated. WCEs and HA immunoprecipitates were blotted with indicated antibodies. CIA, cytosolic iron–sulfur cluster assembly; CIAO3, cytosolic iron–sulfur assembly component 3; CIAO1: probable cytosolic iron-sulfur protein assembly protein CIAO1; CIAO2B: cytosolic iron-sulfur assembly component 2B; CIAO3: cytosolic iron-sulfur assembly component 3; Fe-S, iron–sulfur; MMS19: MMS19 nucleotide excision repair protein homolog; NUBP1: nucleotide-binding protein 1; NUBP2: nucleotide-binding protein 2; WCE, whole cell extract.

Journal: The Journal of Biological Chemistry

Article Title: Iron-regulated assembly of the cytosolic iron–sulfur cluster biogenesis machinery

doi: 10.1016/j.jbc.2022.102094

Figure Lengend Snippet: Fe-S cluster incorporation into CIAO3 controls its interactions. A , schematic representation of Fe-S incorporation in wildtype CIAO3 and mutants. B – D , Flp-In 293 cells that stably express wildtype or mutant CIAO3 were induced with 1 μg/ml doxycycline for 24 h before harvesting. CIAO3 and associated proteins were affinity purified. Two biological replicates were analyzed. Protein abundances were monitored by a targeted proteomic assay containing known CIA components and a subset of prototypical CIA substrates after normalization to CIAO3 levels. Log2 transformed abundance of the co-eluted proteins (Log2Intensiteis) were plotted for the CIA scaffold complex ( B ), the CIA targeting complex ( C ), and selected CIA substrates ( D ). Mean ± SD was indicated. Protein levels of interactors co-eluted with mutant CIAO3 were compared to wildtype. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001. E , Flp-In 293 control cells, cells expressing wildtype 3HA-3FLAG-ERCC2 and cells expressing mutant ERCC2 lacking the MMS19 binding region were induced with 1 μg/ml doxycycline for 24 h. Cell lysates were immunoprecipitated. WCEs and HA immunoprecipitates were blotted with indicated antibodies. CIA, cytosolic iron–sulfur cluster assembly; CIAO3, cytosolic iron–sulfur assembly component 3; CIAO1: probable cytosolic iron-sulfur protein assembly protein CIAO1; CIAO2B: cytosolic iron-sulfur assembly component 2B; CIAO3: cytosolic iron-sulfur assembly component 3; Fe-S, iron–sulfur; MMS19: MMS19 nucleotide excision repair protein homolog; NUBP1: nucleotide-binding protein 1; NUBP2: nucleotide-binding protein 2; WCE, whole cell extract.

Article Snippet: Membranes were blocked with either 5% milk or 5% BSA before blotting with primary antibodies against CIAPIN1 (Santa Cruz Biotechnology sc-271298, Lot #H2317), GLRX3 (Santa Cruz Biotechnology sc-100601 Lot#C1811), CIAO3 (Santa Cruz Biotechnology sc-514078 Lot #K1914 or Sigma Aldrich SAB4502760), NUBP1 (Santa Cruz Biotechnology sc-514175, Lot #0715), NUBP2 (Proteintech 15409-1-AP), MMS19 (Proteintech 16015-1-AP or 66049-1-IG), CIAO1 (Cell Signaling 87027S Lot:1), CIAO2B (Proteintech 20108-1-AP), CIAO2A (Proteintech 20776-1-AP), ERCC2 (Santa Cruz Biotechnology sc-101174, Lot #K0414), FTH1 (Cell Signaling #3998), FLAG (Sigma #F1804 and Proteintech), ISCU1/2 (Proteintech), HIF1α (Bethyl A300–286A), IRP2 (Santa Cruz Biotechnology sc-33682, Lot #B1116), FBXL5 (BioLegend Clone 3F5G12G9 or 10F4H9D12), GAPDH (Proteintech HRP-60004), and TUBA (Proteintech).

Techniques: Stable Transfection, Mutagenesis, Affinity Purification, Proteomic Assay, Transformation Assay, Control, Expressing, Binding Assay, Immunoprecipitation