p nf2 Search Results


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Proteintech p nf2
FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, <t>neurofibromin</t> <t>2</t> <t>(NF2),</t> and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.
P Nf2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Wageningen University and Research calux-assay
FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, <t>neurofibromin</t> <t>2</t> <t>(NF2),</t> and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.
Calux Assay, supplied by Wageningen University and Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, <t>neurofibromin</t> <t>2</t> <t>(NF2),</t> and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.
Compact Fesem Zeiss Merlin, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, <t>neurofibromin</t> <t>2</t> <t>(NF2),</t> and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.
Tmf Tests Alloy A 1, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, <t>neurofibromin</t> <t>2</t> <t>(NF2),</t> and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.
Field Emission Scanning Electron Microscope Carl Zeiss Merlin Compact 6073, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, neurofibromin 2 (NF2), and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.

Journal: Journal of cellular and molecular medicine

Article Title: DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis.

doi: 10.1111/jcmm.70410

Figure Lengend Snippet: FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, neurofibromin 2 (NF2), and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.

Article Snippet: Then, the sections were blocked with goat serum at room temperature for 10 min, and then incubated with the primary antibodies against GPX4 (#Ab125066, Abcam), SLC7A11 (#Ab175186, Abcam), ACSL4 (#Ab155282, Abcam), ZEB1 (#Sc- 515,797, Santa Cruz Biotechnology), E- cadherin (#20874- 1- AP, Proteintech), p- YAP (#29018- 1- AP, Proteintech) and p- NF2 (#28851- 1- AP, Proteintech) overnight at 4°C.

Techniques: CCK-8 Assay, Quantitative RT-PCR, Expressing, Binding Assay, Control

FIGURE 8 | Effect of DDR1 on the levels of proteins involved in ferroptosis, EMT and NF2–YAP pathway in T24 xenografts. Representative IHC staining and quantification of GPX4, SLC7A11, ACSL4, ZEB1, E-cadherin, p-YAP and p-NF2 in T24 xenograft group tumours. N = 3. *p < 0.05 versus control group. *p < 0.05 versus control group. #p < 0.05 versus erastin group. $p < 0.05 versus OE-DDR1 + erastin group.

Journal: Journal of cellular and molecular medicine

Article Title: DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis.

doi: 10.1111/jcmm.70410

Figure Lengend Snippet: FIGURE 8 | Effect of DDR1 on the levels of proteins involved in ferroptosis, EMT and NF2–YAP pathway in T24 xenografts. Representative IHC staining and quantification of GPX4, SLC7A11, ACSL4, ZEB1, E-cadherin, p-YAP and p-NF2 in T24 xenograft group tumours. N = 3. *p < 0.05 versus control group. *p < 0.05 versus control group. #p < 0.05 versus erastin group. $p < 0.05 versus OE-DDR1 + erastin group.

Article Snippet: Then, the sections were blocked with goat serum at room temperature for 10 min, and then incubated with the primary antibodies against GPX4 (#Ab125066, Abcam), SLC7A11 (#Ab175186, Abcam), ACSL4 (#Ab155282, Abcam), ZEB1 (#Sc- 515,797, Santa Cruz Biotechnology), E- cadherin (#20874- 1- AP, Proteintech), p- YAP (#29018- 1- AP, Proteintech) and p- NF2 (#28851- 1- AP, Proteintech) overnight at 4°C.

Techniques: Immunohistochemistry, Control