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Image Search Results
Journal: Journal of Neuroscience
Article Title: Breakdown of Axonal Synaptic Vesicle Precursor Transport by Microglial Nitric Oxide
doi: 10.1523/jneurosci.3887-04.2005
Figure Lengend Snippet: Figure 4. Immunohistochemistry of phosphorylated JNK in cultured hippocampal neurons. a, Neurons were identified by antibodies directed against the axonal marker protein tau (green). Only rare punctate staining of phosphorylated JNK (red) was detected in untreated neurons. Scale bar, 10 m. b, Phosphorylated JNK (red) was observed in most tau (green)-positive axons after treatment of the cultures with 300 M DEA/NONOate for 5 min. Area selected for inset as indicated. Scale bar, 10 m. c, Percentageofaxonsidentifiedbyimmunolabelingwithantibodiesdirectedagainsttauthatshowdouble-labelingforphosphor- ylatedJNK.Neuronsareeitheruntreatedortreatedwith300MDEA/NONOatefor5,10,or15min.Dataarepresentedasmean SEM. **p 0.005.
Article Snippet: Hippocampal neurons cultured for 1 week were fixed with 4% paraformaldehyde, incubated with a mouse monoclonal antibody specific for
Techniques: Immunohistochemistry, Cell Culture, Marker, Staining
Journal: Acta Cirúrgica Brasileira
Article Title: Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin
doi: 10.1590/acb371002
Figure Lengend Snippet: Licorice zinc inhibited UVB-induced JNK/P38MAPK signaling pathway activation. C57BL/6J mice skin was treated with UVB, mice were given licorice zinc (0.65 g/kg, 1.3 g/kg, 2.6 g/kg) and 250mg tranexamic acid once a day. (a) JNK/ P38MAPK signaling pathway-related proteins were analyzed by western blotting using antibodies against JNK, p-JNK, P38MAPK, and p-P38MAPK, β-actin served as a loading control. (b) The ratio of p-JNK/ JNK and p-P38MAPK/ P38MAPK were quantified by western blotting analysis and normalized to control.
Article Snippet: Then, probed with
Techniques: Activation Assay, Western Blot
Journal: Acta Cirúrgica Brasileira
Article Title: Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin
doi: 10.1590/acb371002
Figure Lengend Snippet: Inhibition JNK pathway restrained UVB--induced melanin formation. After B16F10 cells were cultured for 24 h, cells were treated with 0.1 mM α-MSH for 72 h, meanwhile, cells were treated with licorice zinc negative serum, 10% licorice zinc positive serum, and 10% licorice zinc positive serum+Anisomycin. The Control group was cultured with nothing except medium all along. (a) The content of melanin was measured by sodium hydroxide dissolution. (b) JNK/ P38MAPK signaling pathway and melanin productionrelated protein were an alyzed by western blotting using antibodies against JNK, p-JNK, P38MAPK, pP38MAPK, TRP-1, tyrosinase, and MTIF. β-actin served as a loading control. (c) The relative density of TRP1, tyrosinase, and MTIF, and the ratio of p-JNK/ JNK and p-P38MAPK/ P38MAPK were quantified by western blotting analysis and normalized to control.
Article Snippet: Then, probed with
Techniques: Inhibition, Cell Culture, Western Blot