Journal: Neural regeneration research

Article Title: Growth hormone promotes the reconstruction of injured axons in the hypothalamo-neurohypophyseal system.

doi: 10.4103/1673-5374.389358

Figure Lengend Snippet: Figure 2 ｜Central diabetes insipidus and changes in anxiety state post-pituitary stalk electrical lesion. (A–C) Daily water consumption (A), daily urine volume (B), and urine specific gravity (C) indicated triphasic diabetes insipidus. GH treatment promoted water and electrolyte balance post-PEL. (D, E) Marble-burying test at 3 and 7 days post-surgery. (F) OXT serum content was detected by enzyme-linked immunosorbent assay at three different time points (Sham, and 3 and 7 days post-PEL) and two conditions (GH- or NS-treated). Data are expressed as mean ± SEM (n = 5) and were analyzed by two-way analysis of variance (A–C and F) and one- way analysis of variance (D) followed by Tukey’s multiple comparison test (A–E) or Bonferroni’s multiple comparison test (F). *P < 0.05. GH: Growth hormone; NS: normal saline; ns: not significant; OXT: oxytocin; PEL: pituitary stalk electrical lesion; PEL_10d: 10 days post-PEL; PEL_3d: 3 days post-PEL.

Article Snippet: Oxytocin (OXT) content was detected following the manufacturer’s instructions using the OXT enzyme linked immunosorbent assay kit (74-OXYHU-E01, ALPCO, Selam, NH,

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Saline

Journal: International Journal of Molecular Sciences

Article Title: Novel Long-Acting Oxytocin Analog with Increased Efficacy in Reducing Food Intake and Body Weight

doi: 10.3390/ijms231911249

Figure Lengend Snippet: Structure of novel long-acting oxytocin analogue ASK2131. The native oxytocin peptide was modified with a substitution of the Leu8 to a Lys appended with a polyethylene glycol space and a palmitoyl group and with a substitution of Gly for the Pro7 to enhance selectivity for the OXT receptor to create the prior peptide ASK1476. The design of ASK1476 had been further enhanced by replacing its disulfide bridge with a thioether isostere and removing the Cys amino group, to create ASK2131, a novel OXT receptor analog.

Article Snippet: Lyophilized oxytocin (Syntocinon) was purchased from Selleck Chemical (cat. # P1029; Houston, TX, USA) and dissolved in 1 mL if sterile ultra-pure H 2 O.

Techniques: Modification

Journal: International Journal of Molecular Sciences

Article Title: Novel Long-Acting Oxytocin Analog with Increased Efficacy in Reducing Food Intake and Body Weight

doi: 10.3390/ijms231911249

Figure Lengend Snippet: Changes in body weight, food intake, and water intake were tested during 22-day treatment with ASK2131 vs. pair-feeding to ASK2131 vs. oxytocin (OXT) vs. vehicle in diet-induced obese (DIO) male Sprague Dawley rats which were matched based on baseline food intake and body weight gain trajectory. Body weight was reduced in all treatment groups with strongest reductions in percent reductions of body weight ( A , B ), and total body weight ( C ) in rats treated with ASK2131, followed by pair-fed to ASK2131. Daily food intake was reduced during the whole duration of 22-day treatment with ASK2131 in comparison to vehicle ( D ). Water intake was comparable between all treatment groups ( E ). A,D: Data were analyzed by repeated measure two-way ANOVA and Šídák’s correction for multiple comparisons for comparing treatment groups vs. vehicle. # / ## p < 0.05/0.01 ASK2131 vs. OXT. */** p < 0.05/0.01 ASK2131 vs. vehicle. B,C: Analysis by one way ANOVA followed by Šídák’s multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Lyophilized oxytocin (Syntocinon) was purchased from Selleck Chemical (cat. # P1029; Houston, TX, USA) and dissolved in 1 mL if sterile ultra-pure H 2 O.

Techniques: Comparison

Journal: International Journal of Molecular Sciences

Article Title: Novel Long-Acting Oxytocin Analog with Increased Efficacy in Reducing Food Intake and Body Weight

doi: 10.3390/ijms231911249

Figure Lengend Snippet: Analysis of food intake pattern of continuously automatically recorded food intake of singly housed rats in BioDAQ cages. Strong reductions of food intake were noted already at days 1 and 2 during treatment with ASK2131, but not to OXT compared to baseline, both drugs at 50 nmol/kg ( A ). Cumulative food intake throughout the whole experiment was more reduced in response to ASK2131 than to oxytocin, even when doses were increased for oxytocin every 2 days up to 600 nmol/kg while the dose for ASK2131 was kept at 50 nmol/kg/d ( B ). Green—Vehicle; yellow—Oxytocin; purple—ASK2131; White—ambient light data recorded by BioDAQ system.

Article Snippet: Lyophilized oxytocin (Syntocinon) was purchased from Selleck Chemical (cat. # P1029; Houston, TX, USA) and dissolved in 1 mL if sterile ultra-pure H 2 O.

Techniques: