Journal: Archives of Toxicology

Article Title: How the methodology determines the outcome of the in vitro micronucleus assay (OECD TG 487): a comparison of the MicroFlow and the microscopic evaluation approach highlights the impact of cytotoxicity/cytostasis metrics in V79 cells for matrine

doi: 10.1007/s00204-025-04271-1

Figure Lengend Snippet: GFP induction for six marker genes and cell survival after 24 h incubation with oxymatrine in absence and presence of 0.4% S9 mix, given as mean ± SEM, n = 3

Article Snippet: Matrine (MA) and oxymatrine (OM) used for the MicroFlow assay were obtained from MedChemExpress, USA with a purity of ≥ 98.0% for batch ID 27619 (MA) and 25568 (OM).

Techniques: Marker, Incubation

Journal: Frontiers in Pharmacology

Article Title: The Extract of Roots of Sophora flavescens Enhances the Recovery of Motor Function by Axonal Growth in Mice with a Spinal Cord Injury

doi: 10.3389/fphar.2015.00326

Figure Lengend Snippet: LC/MS analysis of the alkaloid and water fractions . Photodiode array (PDA) total scan chromatograms, total ion chromatograms, and extracted ion chromatograms at m/z 249.1967 ± 0.003 and 265.1916 ± 0.003 to detect matrine and oxymatrine, respectively, in the alkaloid fraction and the water fraction. * A specific peak of matrine; # a specific peak of oxymatrine.

Article Snippet: On the next day of the culture, the S. flavescens extract (1 and 10 μg/ml), ethyl acetate fraction (1 and 10 μg/ml), alkaloid fraction (1 and 10 μg/ml), water fraction (1 and 10 μg/ml), matrine (1 and 10 μM; Tokyo Chemical Industry Co., Tokyo, Japan), oxymatrine (1 and 10 μM; Santa Cruz Biotechnology Inc., Dallas, TX, USA), or vehicle solution was applied to the cells.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Frontiers in Pharmacology

Article Title: The Extract of Roots of Sophora flavescens Enhances the Recovery of Motor Function by Axonal Growth in Mice with a Spinal Cord Injury

doi: 10.3389/fphar.2015.00326

Figure Lengend Snippet: Matrine and oxymatrine promote axonal extension on the CSPG coating . Cortical neurons were cultured for 1 day and then treated with matrine (1 and 10 μM), oxymatrine (1 and 10 μM), or vehicle solution. Four days after the treatment, the cells were fixed and immunostained for pNF-H and MAP2. (A) Representative immunofluorescence images of pNF-H for each treatment. (B) The density of pNF-H-positive axons per neuron were quantified for each treatment. The numbers in parentheses indicate the measured number of captured images. * p < 0.05 vs. CSPG coating/vehicle-treated group, One-way ANOVA post hoc Bonferroni test. Scale bar = 200 μm.

Article Snippet: On the next day of the culture, the S. flavescens extract (1 and 10 μg/ml), ethyl acetate fraction (1 and 10 μg/ml), alkaloid fraction (1 and 10 μg/ml), water fraction (1 and 10 μg/ml), matrine (1 and 10 μM; Tokyo Chemical Industry Co., Tokyo, Japan), oxymatrine (1 and 10 μM; Santa Cruz Biotechnology Inc., Dallas, TX, USA), or vehicle solution was applied to the cells.

Techniques: Cell Culture, Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: The Extract of Roots of Sophora flavescens Enhances the Recovery of Motor Function by Axonal Growth in Mice with a Spinal Cord Injury

doi: 10.3389/fphar.2015.00326

Figure Lengend Snippet: Matrine, oxymatrine, and S. flavescens extract have no effects on axonal extension on the PDL coating . Cortical neurons were cultured for 1 day and then treated with matrine (1 and 10 μM), oxymatrine (1 and 10 μM), S. flavescens extract (1 and 10 μg/ml), or vehicle solution. Four days after the treatment, the cells were fixed and immunostained for pNF-H and MAP2. The density of pNF-H-positive axons per neuron were quantified for each treatment. The numbers in parentheses indicate the measured number of captured images.

Article Snippet: On the next day of the culture, the S. flavescens extract (1 and 10 μg/ml), ethyl acetate fraction (1 and 10 μg/ml), alkaloid fraction (1 and 10 μg/ml), water fraction (1 and 10 μg/ml), matrine (1 and 10 μM; Tokyo Chemical Industry Co., Tokyo, Japan), oxymatrine (1 and 10 μM; Santa Cruz Biotechnology Inc., Dallas, TX, USA), or vehicle solution was applied to the cells.

Techniques: Cell Culture

Journal: Bioengineered

Article Title: Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2).

doi: 10.1080/21655979.2022.2078943

Figure Lengend Snippet: Figure 1. OMT reduced circ_0008460 expression in CC cells. (a-b) Circ_0008460 level was assayed via RT-qPCR in CC tissues (a) and CaSki/SiHa cells (b). (c-d) Linear WHSC1 and circ_0008460 levels were detected using RT-qPCR after RNase R treatment for total RNA. (e-f) Circ_0008460 expression was examined through RT-qPCR in control, 2 mg/mL, 4 mg/mL, and 6 mg/mL OMT groups of CaSki and SiHa cells. *P < 0.05.

Article Snippet: CaSki and SiHa cells were exposed to OMT (Selleck, Houston, TX, USA) with 2 mg/mL, 4 mg/mL, or 6 mg/mL.

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Bioengineered

Article Title: Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2).

doi: 10.1080/21655979.2022.2078943

Figure Lengend Snippet: Figure 2. Circ_0008460 abrogated OMT-induced inhibition of CC cell malignant behaviors. (a) RT-qPCR was applied for assessing the overexpression efficiency of oe-circ_0008460. (b-k) CaSki and SiHa cells were divided into control, OMT (6 mg/mL), OMT+vector, and OMT+oe-circ_0008460 groups. (b) CCK-8 assay was used for determining cell viability. (c-d) Colony formation assay (c) and Edu assay (d) were employed for examining proliferation ability. (e) Flow cytometry was exploited for measuring the apoptosis rate. (f-g) Western blot was applied for assaying protein levels of apoptosis-related markers. (h-i) Wound healing assay was used for detecting cell migration. (j-k) Transwell assay was employed for evaluating cell migration (j) and invasion (k). *P < 0.05.

Article Snippet: CaSki and SiHa cells were exposed to OMT (Selleck, Houston, TX, USA) with 2 mg/mL, 4 mg/mL, or 6 mg/mL.

Techniques: Inhibition, Quantitative RT-PCR, Over Expression, Control, Plasmid Preparation, CCK-8 Assay, Colony Assay, EdU Assay, Flow Cytometry, Western Blot, Wound Healing Assay, Migration, Transwell Assay

Journal: Bioengineered

Article Title: Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2).

doi: 10.1080/21655979.2022.2078943

Figure Lengend Snippet: Figure 3. Circ_0008460 served as a natural sponge for miR-197-3p. (a) Venn Diagram analysis of miRNA targets from starbase and circinteractome. (b-c) The miRNA targets for circ_0008460 were screened by pull-down assay. (d) Circ_0008460 and miR-197-3p binding prediction in starbase. (e) The efficacy of miR-197-3p mimic was assessed via RT-qPCR. (f-h) Target binding between circ_0008460 and miR-197-3p was confirmed through dual-luciferase reporter assay (f-g) and RNA pull-down assay (h). (i-k) RT-qPCR was performed for miR-197-3p level quantification in CC tissues (i), CaSki/SiHa cells (j), and 6 mg/mL OMT-treated CC cells (k). NC: normal control, *P < 0.05.

Article Snippet: CaSki and SiHa cells were exposed to OMT (Selleck, Houston, TX, USA) with 2 mg/mL, 4 mg/mL, or 6 mg/mL.

Techniques: Pull Down Assay, Binding Assay, Quantitative RT-PCR, Luciferase, Reporter Assay, Control

Journal: Bioengineered

Article Title: Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2).

doi: 10.1080/21655979.2022.2078943

Figure Lengend Snippet: Figure 4. OMT inhibited CC cell progression by regulating circ_0008460/miR-197-3p axis. CaSki and SiHa cells were treated with control, OMT (6 mg/mL), OMT+vector, OMT+oe-circ_0008460, OMT+oe-circ_0008460+ miR-NC, and OMT+oe-circ_0008460 + miR- 197-3p. (a) Cell viability was measured using CCK-8 assay. (b-c) Cell proliferation was evaluated via colony formation assay (b) and Edu assay (c). (d-f) Cell apoptosis was analyzed through flow cytometry (d) and western blot (e-f). (g-j) Cell motility was assessed by wound healing assay (g-h) and transwell assay (i-j). NC: normal control, *P < 0.05.

Article Snippet: CaSki and SiHa cells were exposed to OMT (Selleck, Houston, TX, USA) with 2 mg/mL, 4 mg/mL, or 6 mg/mL.

Techniques: Control, Plasmid Preparation, CCK-8 Assay, Colony Assay, EdU Assay, Flow Cytometry, Western Blot, Wound Healing Assay, Transwell Assay

Journal: Bioengineered

Article Title: Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2).

doi: 10.1080/21655979.2022.2078943

Figure Lengend Snippet: Figure 5. Circ_0008460 regulated RRM2 expression via sponging miR-197-3p. (a) RRM2 3ʹUTR sequence was predicted to have binding sites with miR-197-3p sequence in starbase. (b-c) Dual-luciferase reporter assay was used for interaction analysis between miR-197-3p and RRM2 3ʹUTR in CaSki and SiHa cells. (d-e) RT-qPCR and western blot were conducted for level examination of RRM2 after miR-NC or miR-197-3p transfection. (f-k) RRM2 mRNA and protein levels were determined in CC samples (f-g), CaSki/SiHa cells (h-i), and OMT-exposed CC cells (j-k) using RT-qPCR and western blot. (l-m) RRM2 mRNA and protein detection was performed via RT-qPCR and western blot in vector, oe-circ_0008460, oe-circ_0008460 + mi-NC, and oe-circ_0008460+ miR-197-3p groups. NC: normal control, *P < 0.05.

Article Snippet: CaSki and SiHa cells were exposed to OMT (Selleck, Houston, TX, USA) with 2 mg/mL, 4 mg/mL, or 6 mg/mL.

Techniques: Expressing, Sequencing, Binding Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation, Control

Journal: Bioengineered

Article Title: Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2).

doi: 10.1080/21655979.2022.2078943

Figure Lengend Snippet: Figure 6. OMT exhibited anti-cancer function in CC cells by upregulating miR-197-3p to downregulate RRM2. (a-b) Transfection efficiencies of anti-miR-197-3p (a) and si-RRM2 (b) were examined by RT-qPCR and western blot, respectively. (c-l) 6 mg/mL-treated CaSki and SiHa cells were transfected with anti-miR-197-3p, anti-miR-197-3p+si-RRM2, or relative controls. (c) CCK-8 assay was performed for viability detection. (d-e) The examination of cell proliferation was conducted using colony formation assay (d) and Edu assay (e). (f-h) Cell apoptosis analysis was implemented through flow cytometry (f) and western blot (g-h). (i-l) Wound healing assay (i-j) and transwell assay (k-l) were performed to assess cell motility. NC: normal control, *P < 0.05.

Article Snippet: CaSki and SiHa cells were exposed to OMT (Selleck, Houston, TX, USA) with 2 mg/mL, 4 mg/mL, or 6 mg/mL.

Techniques: Transfection, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Colony Assay, EdU Assay, Flow Cytometry, Wound Healing Assay, Transwell Assay, Control

Journal: Bioengineered

Article Title: Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2).

doi: 10.1080/21655979.2022.2078943

Figure Lengend Snippet: Figure 7. OMT inhibited tumor growth of CC by downregulating circ_0008460 to mediate miR-197-3p/RRM2 axis in vivo. CaSki xenograft models of lenti-NC, OMT+lenti-NC, and OMT+lenti-circ_0008460 were constructed in mice. (a-b) Tumor volume (a) and weight (b) in nude mice. (c) Images of tumor tissues. (d-e) Circ_0008460 (d) and miR-197-3p (e) levels were assayed through RT- qPCR. (f-g) Western blot and IHC assays were used for protein determination of RRM2 (f) and Ki67 (g) in tumors. NC: normal control, *P < 0.05.

Article Snippet: CaSki and SiHa cells were exposed to OMT (Selleck, Houston, TX, USA) with 2 mg/mL, 4 mg/mL, or 6 mg/mL.

Techniques: In Vivo, Construct, Quantitative RT-PCR, Western Blot, Control

Journal: Journal of Agricultural Chemistry and Environment

Article Title: Quantitative Analysis of Matrine and Oxymatrine in Sophora flavescens Extract and Its Biopesticides by UPLC

doi: 10.4236/jacen.2014.32008

Figure Lengend Snippet: Figure 1. UPLC chromatogram of matrine and oxymatrine in biopesticide.

Article Snippet: Matrine (purity 99.9%) and oxymatrine (purity 94.3%) were purchased from ChromaDex (Irvine, California, USA).

Techniques:

Journal: Journal of Agricultural Chemistry and Environment

Article Title: Quantitative Analysis of Matrine and Oxymatrine in Sophora flavescens Extract and Its Biopesticides by UPLC

doi: 10.4236/jacen.2014.32008

Figure Lengend Snippet: Figure 2. Recoveries of matrine (a) and oxymatrine (b) in the selected six car- tridges with and without DW washing (I: ENVI-Carb 0.25 g, II: ENVI-Carb 0.5 g, III: Activated Carbon 0.5 g, IV: Carbograph 0.5 g, V: Carbograph 1 g, VI: Bond Elut 0.5 g). Vertical bars represent ±SD of the means.

Article Snippet: Matrine (purity 99.9%) and oxymatrine (purity 94.3%) were purchased from ChromaDex (Irvine, California, USA).

Techniques:

Journal: Journal of Agricultural Chemistry and Environment

Article Title: Quantitative Analysis of Matrine and Oxymatrine in Sophora flavescens Extract and Its Biopesticides by UPLC

doi: 10.4236/jacen.2014.32008

Figure Lengend Snippet: Figure 3. UV-Vis spectra of MeOH (a) and acetonitrile (b) eluate of biopesticide sample with and without C18 SPE cartridge clean-up. per insect, respectively. If we only consider the concentration of alkaloidal matrines as bioactive materials, the contents of matrine and oxymatrine in some products among the tested biopesticides were assumed to be suffi- cient as crop protection agents. However, if we consider the practical use guideline of biopesticide, the concen- tration of the active ingredient would be expected to be lower than EC50 and LD50 level, because the general guideline for field application was suggested be 100 - 1000 folds diluted with water. In a survey of matrines present in biopesticides from S. flavescens, the concentration of bioactive materials showed wide ranges and these results would be related to the crop protection efficiency. Thus, the quality control of the commercial bio- pesticide should be performed.

Article Snippet: Matrine (purity 99.9%) and oxymatrine (purity 94.3%) were purchased from ChromaDex (Irvine, California, USA).

Techniques: Concentration Assay, Control

Journal: Journal of Agricultural Chemistry and Environment

Article Title: Quantitative Analysis of Matrine and Oxymatrine in Sophora flavescens Extract and Its Biopesticides by UPLC

doi: 10.4236/jacen.2014.32008

Figure Lengend Snippet: Figure 4. Flow chart for matrine and oxymatrine analyses in biopesticides.

Article Snippet: Matrine (purity 99.9%) and oxymatrine (purity 94.3%) were purchased from ChromaDex (Irvine, California, USA).

Techniques:

Journal: Experimental Biology and Medicine

Article Title: Combination of oxymatrine and diammonium glycyrrhizinate significantly mitigates mice allergic contact dermatitis induced by dinitrofluorobenzene

doi: 10.1177/1535370219864895

Figure Lengend Snippet: Combination of OMT and DG mitigates the DNFB-induced skin inflammation in mice. ICR mice were smeared with DNFB for five times and after first smearing, the mice were randomized and treated intraperitoneally with saline (model), or the indicated doses of OMT and/or DG daily for 14 days. Their body weights, skin inflammation and ear thicknesses were measured longitudinally. (a) The structures of OMT or DG. (b) The body weights of the different groups of mice. (c) Representative images of skin inflammation in different groups of mice (on day 14). (d) The inflammation scores of individual groups of mice. (e) The ear thicknesses. Data are representative images or expressed as the mean values of individual groups (n = 5 per group). The skin inflammatory scores and ear thicknesses of all ACD mice were significantly greater than the control while the skin inflammatory scores and ear thicknesses in all drug-treated groups were significantly less than that in the model group (statistical markers not shown in Figure 1(d) and (e)). Letters on the graphs indicate the following statistical significant group comparisons: aP< 0.05 vs. the control group; bP< 0.05 vs. the model group; cP< 0.05 vs. the DEX 2 mg·kg−1 group; dP< 0.05 vs. the combination (OMT+DG, 45 + 45 mg·kg−1). OMT: oxymatrine; DG: diammonium glycyrrhizinate; DEX: dexamethasone. (A color version of this figure is available in the online journal.)

Article Snippet: Chemicals and drugs Oxymatrine (molecular formula, C 15 H 24 N 2 O 2 ; molecular weight, 264.36; CAS, 16837–52-8; high performance liquid chromatography (HPLC) purity >98%) and diammonium glycyrrhizinate (molecular formula, C 42 H 62 O 16.2 H 3 N; molecular weight, 856.997; CAS, 79165–06-3; HPLC purity >98%) were purchased from Nanjing Zelang Biological Technology (Nanjing, China) and their structures are shown in .

Techniques: Saline, Control

Journal: Diabetes Therapy

Article Title: Targeting Autophagy: A Promising Therapeutic Strategy for Diabetes Mellitus and Diabetic Nephropathy

doi: 10.1007/s13300-024-01641-3

Figure Lengend Snippet: Key findings related to autophagy-associated molecules in DM and DN

Article Snippet: GLP-1 and GLP-1 receptor agonists , Oxymatrine , Styphnolobium Schott , , GLP-1, ROS, AGEs, TGF-β1, eNOS, NADPH Oxidase 4, connective tissue growth factor, KSRP, PETN, Akt, calcium-sensing receptor , [ – ] .

Techniques: Cell Culture, Over Expression, Inhibition, Gene Expression, Activity Assay, Activation Assay, Expressing, Enzyme Activity Assay, Control

Journal: Diabetes Therapy

Article Title: Targeting Autophagy: A Promising Therapeutic Strategy for Diabetes Mellitus and Diabetic Nephropathy

doi: 10.1007/s13300-024-01641-3

Figure Lengend Snippet: Natural substances alleviate DM and DN via autophagy

Article Snippet: GLP-1 and GLP-1 receptor agonists , Oxymatrine , Styphnolobium Schott , , GLP-1, ROS, AGEs, TGF-β1, eNOS, NADPH Oxidase 4, connective tissue growth factor, KSRP, PETN, Akt, calcium-sensing receptor , [ – ] .

Techniques:

Journal: Botanical Studies

Article Title: Karyological, morphological and phytochemical characteristics of medicinal plants Sophora flavescens Aiton grown from seeds collected at different localities

doi: 10.1186/1999-3110-55-5

Figure Lengend Snippet: Comparisons of total phenols, flavonoids, matrine, oxymatrine, ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) in roots of S. flavescens populations a

Article Snippet: Standards of matrine and oxymatrine were obtained from Wako Company (Tokyo, Japan).

Techniques: