overexpression plasmids Search Results


overexpression  (Addgene inc)
92
Addgene inc overexpression
Overexpression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/overexpression/product/Addgene inc
Average 92 stars, based on 1 article reviews
overexpression - by Bioz Stars, 2026-03
92/100 stars
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double-stranded short interfering rna (sirna) for notch3  (Genechem)
90
Genechem double-stranded short interfering rna (sirna) for notch3
Double Stranded Short Interfering Rna (Sirna) For Notch3, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/double-stranded short interfering rna (sirna) for notch3/product/Genechem
Average 90 stars, based on 1 article reviews
double-stranded short interfering rna (sirna) for notch3 - by Bioz Stars, 2026-03
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sirnas for hmga2  (Ribobio co)
90
Ribobio co sirnas for hmga2
Sirnas For Hmga2, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sirnas for hmga2 - by Bioz Stars, 2026-03
90/100 stars
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overexpression plasmid pcdna3.1-rnf216  (Shanghai Shenggong Co)
90
Shanghai Shenggong Co overexpression plasmid pcdna3.1-rnf216
Overexpression Plasmid Pcdna3.1 Rnf216, supplied by Shanghai Shenggong Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/overexpression plasmid pcdna3.1-rnf216/product/Shanghai Shenggong Co
Average 90 stars, based on 1 article reviews
overexpression plasmid pcdna3.1-rnf216 - by Bioz Stars, 2026-03
90/100 stars
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pafr-overexpressing plasmid  (Shanghai Genechem Ltd)
90
Shanghai Genechem Ltd pafr-overexpressing plasmid
Pafr Overexpressing Plasmid, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pafr-overexpressing plasmid/product/Shanghai Genechem Ltd
Average 90 stars, based on 1 article reviews
pafr-overexpressing plasmid - by Bioz Stars, 2026-03
90/100 stars
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plasmid vectors for overexpressing pax3  (Genechem)
90
Genechem plasmid vectors for overexpressing pax3
Plasmid Vectors For Overexpressing Pax3, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid vectors for overexpressing pax3/product/Genechem
Average 90 stars, based on 1 article reviews
plasmid vectors for overexpressing pax3 - by Bioz Stars, 2026-03
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overexpression plasmids (pcdna3.1-nc, pcdna3.1-nrp-1)  (Ribobio co)
90
Ribobio co overexpression plasmids (pcdna3.1-nc, pcdna3.1-nrp-1)
miR-6736-3p directly targets NRP-1 and represses its expression. (A) Predicted binding sites of miR-6736-3p for the 3’UTR of NRP-1, as analyzed by TargetScan v7.2. (B) and (C) Luciferase reporter assay results showed that miR-6736-3p mimics reduced the luciferase activity of NRP-1 wt-3’UTR but not that of the NRP-1 mut-3’UTR vector in MB231/ADM (B) and MCF-7/PTX (C) cells. (D) Biotinylated miR-6736-3p and miR-NC were transfected into MB231/ADM and MCF-7/PTX cells. RNA pull-down assay results showed that NRP-1 mRNA was highly enriched in Bio-miR-6736-3p group. (E) Effects of miR-6736-3p <t>overexpression</t> on the mRNA levels of NRP-1 in MB231/ADM and MCF-7/PTX cells determined by qRT-PCR. (F) Effects of miR-6736-3p overexpression on the protein levels of NRP-1 in MB231/ADM and MCF-7/PTX cells were determined by Western blot. (G) Quantitative analysis of . (H) Relative NRP-1 mRNA expression in MB231/ADM and MCF-7/PTX cells and their parental cells, as detected by qRT-PCR. (I) and (J) Relative NRP-1 mRNA and protein expression in 32 chemotherapy-resistant tissues and 28 chemotherapy-sensitive breast cancer tissues, as detected by qRT-PCR and Western blot. (K) Pearson correlation analysis demonstrated that miR-6736-3p negatively correlated with NRP-1 mRNA expression in breast cancer tissues. ** p < 0.01.
Overexpression Plasmids (Pcdna3.1 Nc, Pcdna3.1 Nrp 1), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/overexpression plasmids (pcdna3.1-nc, pcdna3.1-nrp-1)/product/Ribobio co
Average 90 stars, based on 1 article reviews
overexpression plasmids (pcdna3.1-nc, pcdna3.1-nrp-1) - by Bioz Stars, 2026-03
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single-guide rnas (sgrnas) targeting human bap1 in the plenticrisprv2 backbone  (GenScript corporation)
90
GenScript corporation single-guide rnas (sgrnas) targeting human bap1 in the plenticrisprv2 backbone
miR-6736-3p directly targets NRP-1 and represses its expression. (A) Predicted binding sites of miR-6736-3p for the 3’UTR of NRP-1, as analyzed by TargetScan v7.2. (B) and (C) Luciferase reporter assay results showed that miR-6736-3p mimics reduced the luciferase activity of NRP-1 wt-3’UTR but not that of the NRP-1 mut-3’UTR vector in MB231/ADM (B) and MCF-7/PTX (C) cells. (D) Biotinylated miR-6736-3p and miR-NC were transfected into MB231/ADM and MCF-7/PTX cells. RNA pull-down assay results showed that NRP-1 mRNA was highly enriched in Bio-miR-6736-3p group. (E) Effects of miR-6736-3p <t>overexpression</t> on the mRNA levels of NRP-1 in MB231/ADM and MCF-7/PTX cells determined by qRT-PCR. (F) Effects of miR-6736-3p overexpression on the protein levels of NRP-1 in MB231/ADM and MCF-7/PTX cells were determined by Western blot. (G) Quantitative analysis of . (H) Relative NRP-1 mRNA expression in MB231/ADM and MCF-7/PTX cells and their parental cells, as detected by qRT-PCR. (I) and (J) Relative NRP-1 mRNA and protein expression in 32 chemotherapy-resistant tissues and 28 chemotherapy-sensitive breast cancer tissues, as detected by qRT-PCR and Western blot. (K) Pearson correlation analysis demonstrated that miR-6736-3p negatively correlated with NRP-1 mRNA expression in breast cancer tissues. ** p < 0.01.
Single Guide Rnas (Sgrnas) Targeting Human Bap1 In The Plenticrisprv2 Backbone, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single-guide rnas (sgrnas) targeting human bap1 in the plenticrisprv2 backbone/product/GenScript corporation
Average 90 stars, based on 1 article reviews
single-guide rnas (sgrnas) targeting human bap1 in the plenticrisprv2 backbone - by Bioz Stars, 2026-03
90/100 stars
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shrna targeting sat1/vav3  (Shanghai GenePharma)
90
Shanghai GenePharma shrna targeting sat1/vav3
miR-6736-3p directly targets NRP-1 and represses its expression. (A) Predicted binding sites of miR-6736-3p for the 3’UTR of NRP-1, as analyzed by TargetScan v7.2. (B) and (C) Luciferase reporter assay results showed that miR-6736-3p mimics reduced the luciferase activity of NRP-1 wt-3’UTR but not that of the NRP-1 mut-3’UTR vector in MB231/ADM (B) and MCF-7/PTX (C) cells. (D) Biotinylated miR-6736-3p and miR-NC were transfected into MB231/ADM and MCF-7/PTX cells. RNA pull-down assay results showed that NRP-1 mRNA was highly enriched in Bio-miR-6736-3p group. (E) Effects of miR-6736-3p <t>overexpression</t> on the mRNA levels of NRP-1 in MB231/ADM and MCF-7/PTX cells determined by qRT-PCR. (F) Effects of miR-6736-3p overexpression on the protein levels of NRP-1 in MB231/ADM and MCF-7/PTX cells were determined by Western blot. (G) Quantitative analysis of . (H) Relative NRP-1 mRNA expression in MB231/ADM and MCF-7/PTX cells and their parental cells, as detected by qRT-PCR. (I) and (J) Relative NRP-1 mRNA and protein expression in 32 chemotherapy-resistant tissues and 28 chemotherapy-sensitive breast cancer tissues, as detected by qRT-PCR and Western blot. (K) Pearson correlation analysis demonstrated that miR-6736-3p negatively correlated with NRP-1 mRNA expression in breast cancer tissues. ** p < 0.01.
Shrna Targeting Sat1/Vav3, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/shrna targeting sat1/vav3/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
shrna targeting sat1/vav3 - by Bioz Stars, 2026-03
90/100 stars
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irak1 overexpression plasmid  (Molecular Biosciences Inc)
90
Molecular Biosciences Inc irak1 overexpression plasmid
miR-6736-3p directly targets NRP-1 and represses its expression. (A) Predicted binding sites of miR-6736-3p for the 3’UTR of NRP-1, as analyzed by TargetScan v7.2. (B) and (C) Luciferase reporter assay results showed that miR-6736-3p mimics reduced the luciferase activity of NRP-1 wt-3’UTR but not that of the NRP-1 mut-3’UTR vector in MB231/ADM (B) and MCF-7/PTX (C) cells. (D) Biotinylated miR-6736-3p and miR-NC were transfected into MB231/ADM and MCF-7/PTX cells. RNA pull-down assay results showed that NRP-1 mRNA was highly enriched in Bio-miR-6736-3p group. (E) Effects of miR-6736-3p <t>overexpression</t> on the mRNA levels of NRP-1 in MB231/ADM and MCF-7/PTX cells determined by qRT-PCR. (F) Effects of miR-6736-3p overexpression on the protein levels of NRP-1 in MB231/ADM and MCF-7/PTX cells were determined by Western blot. (G) Quantitative analysis of . (H) Relative NRP-1 mRNA expression in MB231/ADM and MCF-7/PTX cells and their parental cells, as detected by qRT-PCR. (I) and (J) Relative NRP-1 mRNA and protein expression in 32 chemotherapy-resistant tissues and 28 chemotherapy-sensitive breast cancer tissues, as detected by qRT-PCR and Western blot. (K) Pearson correlation analysis demonstrated that miR-6736-3p negatively correlated with NRP-1 mRNA expression in breast cancer tissues. ** p < 0.01.
Irak1 Overexpression Plasmid, supplied by Molecular Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irak1 overexpression plasmid/product/Molecular Biosciences Inc
Average 90 stars, based on 1 article reviews
irak1 overexpression plasmid - by Bioz Stars, 2026-03
90/100 stars
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sirna specific for human adora2a  (Shanghai GenePharma)
90
Shanghai GenePharma sirna specific for human adora2a
miR-6736-3p directly targets NRP-1 and represses its expression. (A) Predicted binding sites of miR-6736-3p for the 3’UTR of NRP-1, as analyzed by TargetScan v7.2. (B) and (C) Luciferase reporter assay results showed that miR-6736-3p mimics reduced the luciferase activity of NRP-1 wt-3’UTR but not that of the NRP-1 mut-3’UTR vector in MB231/ADM (B) and MCF-7/PTX (C) cells. (D) Biotinylated miR-6736-3p and miR-NC were transfected into MB231/ADM and MCF-7/PTX cells. RNA pull-down assay results showed that NRP-1 mRNA was highly enriched in Bio-miR-6736-3p group. (E) Effects of miR-6736-3p <t>overexpression</t> on the mRNA levels of NRP-1 in MB231/ADM and MCF-7/PTX cells determined by qRT-PCR. (F) Effects of miR-6736-3p overexpression on the protein levels of NRP-1 in MB231/ADM and MCF-7/PTX cells were determined by Western blot. (G) Quantitative analysis of . (H) Relative NRP-1 mRNA expression in MB231/ADM and MCF-7/PTX cells and their parental cells, as detected by qRT-PCR. (I) and (J) Relative NRP-1 mRNA and protein expression in 32 chemotherapy-resistant tissues and 28 chemotherapy-sensitive breast cancer tissues, as detected by qRT-PCR and Western blot. (K) Pearson correlation analysis demonstrated that miR-6736-3p negatively correlated with NRP-1 mRNA expression in breast cancer tissues. ** p < 0.01.
Sirna Specific For Human Adora2a, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna specific for human adora2a/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
sirna specific for human adora2a - by Bioz Stars, 2026-03
90/100 stars
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14-3-3zeta overexpression plasmid  (Shanghai GenePharma)
90
Shanghai GenePharma 14-3-3zeta overexpression plasmid
miR-6736-3p directly targets NRP-1 and represses its expression. (A) Predicted binding sites of miR-6736-3p for the 3’UTR of NRP-1, as analyzed by TargetScan v7.2. (B) and (C) Luciferase reporter assay results showed that miR-6736-3p mimics reduced the luciferase activity of NRP-1 wt-3’UTR but not that of the NRP-1 mut-3’UTR vector in MB231/ADM (B) and MCF-7/PTX (C) cells. (D) Biotinylated miR-6736-3p and miR-NC were transfected into MB231/ADM and MCF-7/PTX cells. RNA pull-down assay results showed that NRP-1 mRNA was highly enriched in Bio-miR-6736-3p group. (E) Effects of miR-6736-3p <t>overexpression</t> on the mRNA levels of NRP-1 in MB231/ADM and MCF-7/PTX cells determined by qRT-PCR. (F) Effects of miR-6736-3p overexpression on the protein levels of NRP-1 in MB231/ADM and MCF-7/PTX cells were determined by Western blot. (G) Quantitative analysis of . (H) Relative NRP-1 mRNA expression in MB231/ADM and MCF-7/PTX cells and their parental cells, as detected by qRT-PCR. (I) and (J) Relative NRP-1 mRNA and protein expression in 32 chemotherapy-resistant tissues and 28 chemotherapy-sensitive breast cancer tissues, as detected by qRT-PCR and Western blot. (K) Pearson correlation analysis demonstrated that miR-6736-3p negatively correlated with NRP-1 mRNA expression in breast cancer tissues. ** p < 0.01.
14 3 3zeta Overexpression Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/14-3-3zeta overexpression plasmid/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
14-3-3zeta overexpression plasmid - by Bioz Stars, 2026-03
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Image Search Results


miR-6736-3p directly targets NRP-1 and represses its expression. (A) Predicted binding sites of miR-6736-3p for the 3’UTR of NRP-1, as analyzed by TargetScan v7.2. (B) and (C) Luciferase reporter assay results showed that miR-6736-3p mimics reduced the luciferase activity of NRP-1 wt-3’UTR but not that of the NRP-1 mut-3’UTR vector in MB231/ADM (B) and MCF-7/PTX (C) cells. (D) Biotinylated miR-6736-3p and miR-NC were transfected into MB231/ADM and MCF-7/PTX cells. RNA pull-down assay results showed that NRP-1 mRNA was highly enriched in Bio-miR-6736-3p group. (E) Effects of miR-6736-3p overexpression on the mRNA levels of NRP-1 in MB231/ADM and MCF-7/PTX cells determined by qRT-PCR. (F) Effects of miR-6736-3p overexpression on the protein levels of NRP-1 in MB231/ADM and MCF-7/PTX cells were determined by Western blot. (G) Quantitative analysis of . (H) Relative NRP-1 mRNA expression in MB231/ADM and MCF-7/PTX cells and their parental cells, as detected by qRT-PCR. (I) and (J) Relative NRP-1 mRNA and protein expression in 32 chemotherapy-resistant tissues and 28 chemotherapy-sensitive breast cancer tissues, as detected by qRT-PCR and Western blot. (K) Pearson correlation analysis demonstrated that miR-6736-3p negatively correlated with NRP-1 mRNA expression in breast cancer tissues. ** p < 0.01.

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: Long non-coding RNA RP11-70C1.3 confers chemoresistance of breast cancer cells through miR-6736-3p/NRP-1 axis

doi: 10.17305/bjbms.2021.5803

Figure Lengend Snippet: miR-6736-3p directly targets NRP-1 and represses its expression. (A) Predicted binding sites of miR-6736-3p for the 3’UTR of NRP-1, as analyzed by TargetScan v7.2. (B) and (C) Luciferase reporter assay results showed that miR-6736-3p mimics reduced the luciferase activity of NRP-1 wt-3’UTR but not that of the NRP-1 mut-3’UTR vector in MB231/ADM (B) and MCF-7/PTX (C) cells. (D) Biotinylated miR-6736-3p and miR-NC were transfected into MB231/ADM and MCF-7/PTX cells. RNA pull-down assay results showed that NRP-1 mRNA was highly enriched in Bio-miR-6736-3p group. (E) Effects of miR-6736-3p overexpression on the mRNA levels of NRP-1 in MB231/ADM and MCF-7/PTX cells determined by qRT-PCR. (F) Effects of miR-6736-3p overexpression on the protein levels of NRP-1 in MB231/ADM and MCF-7/PTX cells were determined by Western blot. (G) Quantitative analysis of . (H) Relative NRP-1 mRNA expression in MB231/ADM and MCF-7/PTX cells and their parental cells, as detected by qRT-PCR. (I) and (J) Relative NRP-1 mRNA and protein expression in 32 chemotherapy-resistant tissues and 28 chemotherapy-sensitive breast cancer tissues, as detected by qRT-PCR and Western blot. (K) Pearson correlation analysis demonstrated that miR-6736-3p negatively correlated with NRP-1 mRNA expression in breast cancer tissues. ** p < 0.01.

Article Snippet: The small interfering RNAs (siRNAs; si-NC, si-RP11-70C1.3), short hairpin RNA (shRNA; sh-NC, sh-RP11-70C1.3), miR-6736-3p mimics, miR-6736-3p inhibitor, miR-NC, and overexpression plasmids (pcDNA3.1-NC, pcDNA3.1-NRP-1) were synthesized by RiboBio (Guangdong, China).

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay, Activity Assay, Plasmid Preparation, Transfection, Pull Down Assay, Over Expression, Quantitative RT-PCR, Western Blot

RP11-70C1.3 promotes breast cancer chemoresistance through regulating miR-6736-3p/NRP-1 axis. (A) and (B) miR-6736-3p inhibitors reversed the suppression of RP11-70C1.3 knockdown on NRP-1 mRNA (A) and protein (B) level in both MB231/ADM and MCF-7/PTX cells. (C) Pearson correlation analysis demonstrated that RP11-70C1.3 positively correlated with NRP-1 mRNA expression in breast cancer tissues. (D) and (E) qRT-PCR and Western blot were used to validate the upregulation of NRP-1 in mRNA (D) and protein (F) levels in MB231/ADM and MCF-7/PTX cells after transfected with pcDNA3.1-NRP-1 or its negative control pcDNA3.1. (F) and (G) CCK-8 assay results showed that transfection of miR-6736-3p inhibitor or pcDNA3.1-NRP-1 attenuated the chemoresistance inhibition induced by RP11-70C1.3 knockdown in MB231/ADM (F) and MCF-7/PTX (G) cells. (H) The apoptosis rate induced by RP11-70C1.3 knockdown in MB231/ADM and MCF-7/PTX cells was obviously decreased after transfection with miR-6736-3p inhibitor or pcDNA3.1-NRP-1. (I) The effects of RP11-70C1.3 knockdown on the capability of colony formation in MB231/ADM and MCF-7/PTX cells were reversed after miR-6736-3p silence or NRP-1 overexpression. * p < 0.05; ** p < 0.01.

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: Long non-coding RNA RP11-70C1.3 confers chemoresistance of breast cancer cells through miR-6736-3p/NRP-1 axis

doi: 10.17305/bjbms.2021.5803

Figure Lengend Snippet: RP11-70C1.3 promotes breast cancer chemoresistance through regulating miR-6736-3p/NRP-1 axis. (A) and (B) miR-6736-3p inhibitors reversed the suppression of RP11-70C1.3 knockdown on NRP-1 mRNA (A) and protein (B) level in both MB231/ADM and MCF-7/PTX cells. (C) Pearson correlation analysis demonstrated that RP11-70C1.3 positively correlated with NRP-1 mRNA expression in breast cancer tissues. (D) and (E) qRT-PCR and Western blot were used to validate the upregulation of NRP-1 in mRNA (D) and protein (F) levels in MB231/ADM and MCF-7/PTX cells after transfected with pcDNA3.1-NRP-1 or its negative control pcDNA3.1. (F) and (G) CCK-8 assay results showed that transfection of miR-6736-3p inhibitor or pcDNA3.1-NRP-1 attenuated the chemoresistance inhibition induced by RP11-70C1.3 knockdown in MB231/ADM (F) and MCF-7/PTX (G) cells. (H) The apoptosis rate induced by RP11-70C1.3 knockdown in MB231/ADM and MCF-7/PTX cells was obviously decreased after transfection with miR-6736-3p inhibitor or pcDNA3.1-NRP-1. (I) The effects of RP11-70C1.3 knockdown on the capability of colony formation in MB231/ADM and MCF-7/PTX cells were reversed after miR-6736-3p silence or NRP-1 overexpression. * p < 0.05; ** p < 0.01.

Article Snippet: The small interfering RNAs (siRNAs; si-NC, si-RP11-70C1.3), short hairpin RNA (shRNA; sh-NC, sh-RP11-70C1.3), miR-6736-3p mimics, miR-6736-3p inhibitor, miR-NC, and overexpression plasmids (pcDNA3.1-NC, pcDNA3.1-NRP-1) were synthesized by RiboBio (Guangdong, China).

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Transfection, Negative Control, CCK-8 Assay, Inhibition, Over Expression