Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a Schematic representation of adipose tissue-mimicking collagen-based organo-hydrogels (OHGs). b BODIPY (green) staining of human peritoneal adipose tissue and OHG (representative images from n = 7 patients and n = 3 OHGs). c Quantification of human peritoneal adipocyte ( n = 100 adipocytes/tissue from 7 patients) and silicone oil microdroplet ( n = 100 microdroplets from 3 OHGs) diameter. d Quantification of volume fraction occupied by oil in peritoneal adipose tissues ( n = 7 patients) and OHGs ( n = 3 gels). e Storage moduli of hydrogels and human peritoneal tissues ( n = 5 gels; n = 9 adipose and n = 5 connective tissues). f Normalised stress-relaxation curves of hydrogels and human peritoneal tissues ( n = 5 gels or tissues). g Spheroid area after 7 d relative to day 0 of multiple ovarian cancer cell lines ( n = 3 spheroids). h Spheroid area comparison of ovarian cancer cell lines after 7 d in collagen or OHGs relative to the collagen average ( n = 3 spheroids). i Collagen-I (grey) and mRFP-OVCAR8 nuclei (red) staining of OVCAR8 cells seeded on top of collagen or OHG at 25 μm gel depth after 7 d in culture (representative images from n = 3 experiments). j Quantification of hydrogel organotypic invasion of OVCAR8 cells after 7 d ( n = 3 experiments). Rhombuses indicate the average. k BODIPY (green), mRFP-OVCAR8 (red), and collagen I (grey) staining of peritoneal tissue explants and mRFP-OVCAR8 cells after 7 d in culture (representative images from n = 3 experiments). Arrowheads indicate invading mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells in peritoneal tissue explants and OHGs (42 μm depth) after 7 d culture (representative images from n = 3 experiments). n Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues or OHGs after 7 d ( n = 3 tissues from distinct donors or gels). Rhombuses indicate the average. For the data in ( c , d , g , h , j , l and n ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( e ). Error bars in ( e ) represent the s.e.m. Scale bars, 50 μm ( b , i , m ), 100 μm ( k ). Components of ( a ) have been created in BioRender. Gautrot, J. (2025) https://BioRender.com/rk3jchz . Source data are provided as a Source Data file.

Article Snippet: OVCAR3 (HTB-161, ATCC), OVCAR8 (305383, Cytion), OVCAR4 (SCC258, Sigma), Kuramochi (JCRB0098, JCRB) and CAOV3 (HTB-75, ATCC) cells were grown in RPMI medium, while Tyk-nu (JCRB0234.0, JCRB) and Tyk-nu.CPR (JCRB0234.1, JCRB) were cultured in Minimum Essential Medium (MEM).

Techniques: Staining, Comparison

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a pFAK, pMLC, YAP/TAZ (green; left to right) and nuclei (blue) staining of OVCAR8 cells in collagen or organo-hydrogel (OHG) after 48 h in culture (representative images from n = 3 gels). b Quantification of immunofluorescence signal intensity of cytoplasmic pFAK, pMLC, relative to the average in collagen and cytoplasmic:nuclear ratio of YAP/TAZ in OVCAR8 cells embedded in collagen or OHG for 48 h ( n = 24 cells). c F-actin (yellow), nucleus (blue) and BODIPY (grey) staining of an OVCAR8 cell spread at the ECM-microdroplet interface. d Shape descriptors of OVCAR8 nuclei after 24 h in collagen or OHG ( n = 53 nuclei). e Relative spheroid area of OVCAR8 after 7 d in OHG ( n = 10 spheroids). f BODIPY (green) and mRFP (red) staining of peritoneal adipose tissue explants and mRFP-OVCAR8 cells (49 μm into the tissue from the surface) after 7 d in culture (representative images from n = 3 experiments). g Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. h Nuclei (red) of OVCAR8 cells in OHGs after 7 d treatment (representative images from n = 14 spheroids). i Quantification of spheroid area relative to DMSO control of OVCAR8 cells in OHGs ( n = 14 spheroids). i F-actin (green) and nuclei staining of CAOV3 spheroids embedded in OHGs for 7 d (representative images from n = 6 spheroids). k Quantification of spheroid area in CAOV3 cells in OHGs after 7 d ( n = 6 spheroids). l F-actin (green) and nuclei (blue) staining of Kuramochi spheroids in collagen or OHG for 7 d with or without TGFβ (representative images from n = 3 spheroids). For the data in ( b , d , g , i and k ), a two-sided unpaired t test was performed. For data in ( e ), a one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed. Scale bars, 25 μm ( a , c ), 100 μm ( f , I ), 200 μm ( h , j ). Source data are provided as a Source Data file.

Article Snippet: OVCAR3 (HTB-161, ATCC), OVCAR8 (305383, Cytion), OVCAR4 (SCC258, Sigma), Kuramochi (JCRB0098, JCRB) and CAOV3 (HTB-75, ATCC) cells were grown in RPMI medium, while Tyk-nu (JCRB0234.0, JCRB) and Tyk-nu.CPR (JCRB0234.1, JCRB) were cultured in Minimum Essential Medium (MEM).

Techniques: Staining, Immunofluorescence, Control

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a Schematic representation of norbornene-functionalised hyaluronic acid (NB-HA)-based organohydrogels (OHGs) presenting matrix metalloproteinase (MMP)-degradable crosslinking peptides and cell adhesion ligands. b Storage moduli of collagen- and NB-HA-based OHGs ( n = 5 collagen-based and n = 3 NB-HA-based OHGs; average ± s.e.m.). c Normalised stress-relaxation curves of collagen- and HA-NB OHG ( n = 5 collagen-based and n = 3 NB-HA-based OHGs). d F-actin (grey) and nuclei (red) in OVCAR8 cells embedded in collagen- or NB-HA-based OHGs for 7 d (representative images from n = 9 spheroids). e OVCAR8 spheroid area in collagen- or NB-HA-based OHGs relative to collagen-based OHG average ( n = 9 spheroids). f F-actin (grey) and nuclei (red) of OVCAR8 cells embedded in GFOGER- or RGD-presenting NB-HA OHG (representative images from n = 9 spheroids). g BODIPY (green), nuclei (red), and F-actin (grey) of OVCAR8 in GFOGER- or RGD-presenting NB-HA OHG (representative images from n = 9 spheroids). Arrowheads indicate cells in direct contact with oil microdroplets. h OVCAR8 spheroid area in GFOGER- or RGD-presenting NB-HA OHG after 7 d culture relative to GFOGER OHG average ( n = 9 spheroids). i F-actin (grey) and nuclei (red) in OVCAR8 cells embedded in collagen-based OHGs for 7 d (representative images from n = 8 spheroids). j OVCAR8 spheroid area in collagen-based OHGs after 7 d culture relative to DMSO control ( n = 8 spheroids). k BODIPY (green) and mRFP (red) staining of peritoneal adipose tissue explants and mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion depth into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m OVCAR8 spheroid area quantifications in MMP-cleavable or non-cleavable NB-HA OHG after 7 d culture relative to MMP-cleavable control ( n = 11 spheroids). For the data in ( b , e , h , j and l ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( m ). Error bars in ( b ) represent the s.e.m. Scale bars, 100 μm ( g , k ), 200 μm ( d , f , i ). Source data are provided as a Source Data file.

Article Snippet: OVCAR3 (HTB-161, ATCC), OVCAR8 (305383, Cytion), OVCAR4 (SCC258, Sigma), Kuramochi (JCRB0098, JCRB) and CAOV3 (HTB-75, ATCC) cells were grown in RPMI medium, while Tyk-nu (JCRB0234.0, JCRB) and Tyk-nu.CPR (JCRB0234.1, JCRB) were cultured in Minimum Essential Medium (MEM).

Techniques: Control, Staining

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a F-actin (grey) and DAPI (blue) in OVCAR8 cells embedded in collagen or collagen-based organo-hydrogels (OHG) for 24 h (representative images from n = 3 gels). Microdroplet locations are indicated with an asterisk. b BODIPY (green), F-actin (grey) and nuclei (blue) staining of OVCAR8 cells embedded in collagen-based OHG (left; n = 3 gels) or BODIPY (green), mRFP (red) staining of mRFP-OVCAR8 cells invading into peritoneal adipose tissue (right; n = 3 tissues from 1 donor). Arrowheads indicate oil microdroplet (left) and adipocyte (right) deformations at the contact points with cells. c Quantification of OVCAR8 organotypic invasion depth into peritoneal adipose tissues after 7 d ( n = 3 tissues from 1 donor). Rhombuses indicate the average. d Schematic representation of the Sylgard 184 PDMS-based OGH. e F-actin (grey) and nuclei (red) staining of OVCAR8 spheroids embedded for 7 d in collagen-based OHGs prepared with PDMS microdroplets of varying stiffness (representative images from n = 10 spheroids). f OVCAR8 spheroid area after 7 d in collagen-based OHGs with PDMS microdroplets prepared with varying crosslinker percentage, relative to average area in 2 wt% microbead OHGs ( n = 10 spheroids). g Schematic representation of the norbornene-functionalised hyaluronic acid (NB-HA) OGH with norbornene-functionalised bovine serum albumin (NB-BSA) used for RGD presentation at the microdroplet surface. h F-actin (grey) and nuclei (red) of OVCAR8 spheroids after 7 d in NB-HA OHG with localised presentation of RGD (left). Relative OVCAR8 spheroid area in NB-HA OHG with localised RGD presentation after 7 d in culture ( n = 11 spheroids; top right). BODIPY (green), nuclei (red), and F-actin (grey) staining of OVCAR8 cells in NB-HA OHG presenting RGD on the microdroplet surface (bottom right). Arrowhead indicates a cell spreading at the microdroplet-HA interface. i Schematic representation of the system to modulate microdroplet interfacial mechanics. j F-actin (grey) and nuclei (red) of OVCAR8 spheroids after 7 d in NB-HA OHGs (microdroplet-restricted RGD presentation) with varying microdroplet interfacial modulus (representative images from n = 9 spheroids). k OVCAR8 spheroid area in NB-HA OHGs with microdroplet-presenting RGD and varying protein nanosheet interfacial mechanics after 7 d in culture, relative to BSA-only control ( n = 9 spheroids). For the data in ( c and h ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( f and k ). Scale bars, 25 μm ( a , b ), 50 μm ( h , bottom right), 200 μm ( e , h left, j ). Source data are provided as a Source Data file.

Article Snippet: OVCAR3 (HTB-161, ATCC), OVCAR8 (305383, Cytion), OVCAR4 (SCC258, Sigma), Kuramochi (JCRB0098, JCRB) and CAOV3 (HTB-75, ATCC) cells were grown in RPMI medium, while Tyk-nu (JCRB0234.0, JCRB) and Tyk-nu.CPR (JCRB0234.1, JCRB) were cultured in Minimum Essential Medium (MEM).

Techniques: Staining, Control

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a Schematic representation of the organo-hydrogels (OHGs) with varying microdroplet diameter and volume fraction. b Storage modulus of collagen-based OHG with distinct microdroplet size ( n = 4 gels; average ± s.e.m.). The dashed line shows the predicted trend. c Normalised stress-relaxation curves of collagen-based OHG with distinct microdroplet size ( n = 4 gels). d BODIPY (green) and F-actin (red) and nuclei (blue) staining of OVCAR8 cells after 7 d in collagen-based OHG of 70 or 25 μm diameter microdroplets (representative images from n = 13 spheroids). Arrowheads indicate cells at the invasive front. e Quantification of relative OVCAR8 spheroid area in collagen-based OHG of distinct emulsion percentage of volume fraction and microdroplet diameter after 7 d in culture ( n = 13 spheroids). f BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells after 7 d invasion into adipose peritoneal tissue explants (representative images from n = 3 explants per donor). g Correlation between patient average adipocyte diameter and invaded OVCAR8 cells after 7 d, at 42 μm tissue depth, relative to number of cells at the tissue surface ( n = 6 donors; average ± s.e.m.). The dashed line shows the predicted trend. h Quantification of interdroplet distance in OHG ( n = 100 microdroplets). i Quantification of the percentage of invasive front OVCAR8 cells in contact with microdroplets in OHG ( n = 121 cells in 3 gels). j Quantification of percentage of Ki67 + cells, immunofluorescence signal intensity of cytoplasmic pFAK and pMLC relative to collagen average, and nuclear:cytoplasmic ratio of YAP/TAZ in OVCAR8 cells embedded in OHG of 70 or 25 μm diameter microdroplets for 24 h ( n = 55 cells). k F-actin (green) and DAPI (blue) staining of CAOV3 spheroids embedded for 7 d in collagen-based 25 μm microdroplet OHG in the presence or absence of TGFβ. Arrowheads indicate invading cells (representative images from n = 6 spheroids). l Quantification of CAOV3 spheroid area relative to non-TGFβ-treated control after 7 d in culture ( n = 6 spheroids). m Schematic illustration of the cell force-dependent invasion of adipose tissue enabled by the anisotropic mechanics of OHGs and the generation of migration tracks at the ECM-adipocyte/microdroplet interface. Arrows indicate the direction of the force. For the data in ( h , i , j and l ), a two-sided unpaired t test was performed. Coefficient of determination and Pearson correlation (two-tailed test) were performed in ( g ) to determine the relationship between tissue adipocyte diameter and OVCAR8 invasion. Error bars in ( b ) represent the s.e.m. Scale bars, 50 μm (bottom panels in d , k ), 100 μm ( f ), 200 μm (top panels in d , k ). Source data are provided as a Source Data file.

Article Snippet: OVCAR3 (HTB-161, ATCC), OVCAR8 (305383, Cytion), OVCAR4 (SCC258, Sigma), Kuramochi (JCRB0098, JCRB) and CAOV3 (HTB-75, ATCC) cells were grown in RPMI medium, while Tyk-nu (JCRB0234.0, JCRB) and Tyk-nu.CPR (JCRB0234.1, JCRB) were cultured in Minimum Essential Medium (MEM).

Techniques: Staining, Emulsion, Immunofluorescence, Control, Migration, Two Tailed Test

Journal: Oncotarget

Article Title: Immunotherapy of ovarian cancer with a monoclonal antibody specific for the extracellular domain of anti-Müllerian hormone receptor II

doi: 10.18632/oncotarget.27585

Figure Lengend Snippet: A panel of approximately 300 hybridoma supernatants were generated and screened for specificity against rhAMHR2-ED and the 4D12 parental hybridoma was selected for subcloning by limiting dilution. ( A ) Subcloning produced three sub-clones, 4D12C6, 4D12C7, and 4D12G1 each of which expressed the IgG 1 /κ-chain isotype and showed antigen specificity ( B ) by competitive ELISA and ( C ) by flow cytometry binding to OVCAR8 cells. For flow cytometry, positive control staining of OVCAR8 cells was performed using a commercially available anti-AMHR2-ED mAb (Abcam), whereas IgG1 isotype antibodies with irrelevant specificities were used as negative controls. In all cases, error bars indicate ± SD and the results shown are representative of three experiments yielding similar results.

Article Snippet: The AMHR2-OVCAR8 cell line was generated by cloning transcript variant 1 of human AMHR2 (Accession #NM_020547; Origene, Rockville, MD) into pEZ-M68 vector (GeneCopoeia, Rockville, MD) and using this expression vector to transfect OVCAR8 cells using Lipofectamine™ 3000 (Thermo Fisher).

Techniques: Generated, Subcloning, Produced, Clone Assay, Competitive ELISA, Flow Cytometry, Binding Assay, Positive Control, Staining

Journal: Oncotarget

Article Title: Immunotherapy of ovarian cancer with a monoclonal antibody specific for the extracellular domain of anti-Müllerian hormone receptor II

doi: 10.18632/oncotarget.27585

Figure Lengend Snippet: ( A ) Flow cytometry analysis showing that the 4D12G1 mAb binds to the majority of cells generated from two primary HGSOC tissues examined. Error bars indicate ± SD. ( B ) The 4D12G1 mAb was used in Western blots of seven different HGSOC tissue lysates (25 μg protein/lane) with a positive control lysate generated from a young C57BL/6 ovary and a negative control lysate generated from C4-2 human prostate cancer cells. Immunostaining with a β-actin antibody was used to confirm normalized lysate loading. The Western blots shown are representative of three experiments that provided similar results. ( C ) The 4D12G1 mAb was used in immunohistochemical staining (20 ×) of tissue sections from four HGSOC patients (left column) and their normal adjacent fallopian tube tissues (right column). Arrows indicate staining of the tumor parenchyma. The stromal areas of the EOC tumors were not immunostained nor were all areas of the normal adjacent fallopian tube tissues. All experiments were performed three times yielding similar results. ( D ) Western blot analysis of lysates from OVCAR8 cells and AMHR2-OVCAR8 cells with lysates from C4-2 prostate cancer cells used as controls and immunostaining with a β-actin antibody was used to confirm normalized lysate loading. Flow cytometry analysis showed that: ( E ) the 4D12G1 mAb binds to 91% of AMHR2-OVCAR8 cells; ( F ) the AMH cognate ligand for AMHR2-ED effectively competes in a dose-dependent manner with the 4D12G1 mAb for binding to AMHR2-OVCAR8 cells; and ( G ) recombinant ovalbumin failed to compete with the 4D12G1 mAb for binding to AMHR2-OVCAR8 cells. Data are representative of three independent experiments yielding similar results.

Article Snippet: The AMHR2-OVCAR8 cell line was generated by cloning transcript variant 1 of human AMHR2 (Accession #NM_020547; Origene, Rockville, MD) into pEZ-M68 vector (GeneCopoeia, Rockville, MD) and using this expression vector to transfect OVCAR8 cells using Lipofectamine™ 3000 (Thermo Fisher).

Techniques: Flow Cytometry, Generated, Western Blot, Positive Control, Negative Control, Immunostaining, Immunohistochemical staining, Staining, Binding Assay, Recombinant

Journal: Oncotarget

Article Title: Immunotherapy of ovarian cancer with a monoclonal antibody specific for the extracellular domain of anti-Müllerian hormone receptor II

doi: 10.18632/oncotarget.27585

Figure Lengend Snippet: Details of EOC patients and their examined tumors

Article Snippet: The AMHR2-OVCAR8 cell line was generated by cloning transcript variant 1 of human AMHR2 (Accession #NM_020547; Origene, Rockville, MD) into pEZ-M68 vector (GeneCopoeia, Rockville, MD) and using this expression vector to transfect OVCAR8 cells using Lipofectamine™ 3000 (Thermo Fisher).

Techniques: Immunohistochemical staining, Expressing

Journal: Oncotarget

Article Title: Immunotherapy of ovarian cancer with a monoclonal antibody specific for the extracellular domain of anti-Müllerian hormone receptor II

doi: 10.18632/oncotarget.27585

Figure Lengend Snippet: ( A ) The entire 132 amino acid sequence of human AMHR2-ED. ( B ) An overlapping series of 16-mer peptides spanning the entire sequence of human AMHR2-ED with one amino acid shifts were plated for direct ELISA testing using the 4D12G1 mAb as the primary antibody. The 4D12G1 mAb recognized residues AMHR2-ED 11–32. ( C ) Overlapping peptides spanning AMHR2-ED 13–30 were synthesized with alanine substitutions at each N-terminal residue or with glycine substitutions for any native N-terminal alanine residues. Competitive ELISA results showed that alanine substitutions at residues spanning AMHR2-ED 20-26 ( 20 KTLGELL 26 ) decreased binding of the 4D12G1 mAb to AMHR2-ED. ( D ) SPOT peptide arrays using 4-16-mer peptides spanning AMHR2-ED 9-40 were immobilized on cellulose membranes, treated with the 4D12G1 mAb, and the bound antibody was detected by chemiluminescence. The results showed that the AMHR2-ED 22–26 5-mer sequence ( 22 LGELL 26 ) represents the minimal sequence for binding of the 4D12G1 mAb. ( E ) SPOT peptide arrays were made using membrane bound 17-mer peptides spanning the AMHR2-ED 17–33 domain and containing alanine substitutions at each sequential amino acid. Alanine replacement of Leu 22 , Gly 23 , and Leu 26 completely abolished binding by the 4D12G1 mAb. All error bars indicate ±SD, and all experiments are representative of three experiments yielding similar data.

Article Snippet: The AMHR2-OVCAR8 cell line was generated by cloning transcript variant 1 of human AMHR2 (Accession #NM_020547; Origene, Rockville, MD) into pEZ-M68 vector (GeneCopoeia, Rockville, MD) and using this expression vector to transfect OVCAR8 cells using Lipofectamine™ 3000 (Thermo Fisher).

Techniques: Sequencing, Direct ELISA, Synthesized, Competitive ELISA, Binding Assay

Journal: Oncotarget

Article Title: Immunotherapy of ovarian cancer with a monoclonal antibody specific for the extracellular domain of anti-Müllerian hormone receptor II

doi: 10.18632/oncotarget.27585

Figure Lengend Snippet: ( A ) AMHR2-OVCAR8 cells were treated with a green fluorescent dye and the 4D12G1 mAb or an isotype control mAb. Apoptosis was assessed by live imaging using the IncuCyte S3 analyzer. 4D12G1 mAb induced substantial apoptosis at 16 hours (right panel) compared to isotype control mAb (left panel). ( B ) AMHR2-OVCAR8 cells were treated with different concentrations of the 4D12G1 mAb for 24 hours and Western blots of the cell lysates showed detection of the intact 116 kDa PARP-1 and its 89 kDa cleaved variant, consistent with apoptosis. Immunostaining with a β-actin antibody was used to confirm normalized lysate loading. ( C ) AMHR2-OVCAR8 cells were incubated with the 4D12G1 mAb for different time periods at either 37° C (left column) or 4° C (right column). Clustered patterns of cytoplasmic antibody-receptor complexes became increasingly more prominent at 2 and 3 hours after treatment at 37° C, but not at 4° C, and no staining occurred in cells treated with secondary antibody alone (right column, bottom panel). ( D ) AMHR2-OVCAR8 cells were incubated in either 10% normal human serum or 10% heat-inactivated human serum and treated for 4 hours with varying doses of either 4D12G1 mAb or isotype control mAb. Cell lysis mediated by CDC was measured by release of LDH activity and occurred only in cells treated with the 4D12G1 mAb. ( E ) AMHR2-OVCAR8 target cells were labeled with a green fluorescent dye and incubated with two different concentrations of 4D12G1 mAb or isotype control mAb. The cells were mixed with effector macrophages from C57BL/6 mouse bone marrow at an effector to target cell ratio of 10:1. Live target cells were analyzed by flow cytometry 3 days later for demonstrating ADCP. All error bars indicate ±SD. All experiments are representative of three experiments yielding similar data.

Article Snippet: The AMHR2-OVCAR8 cell line was generated by cloning transcript variant 1 of human AMHR2 (Accession #NM_020547; Origene, Rockville, MD) into pEZ-M68 vector (GeneCopoeia, Rockville, MD) and using this expression vector to transfect OVCAR8 cells using Lipofectamine™ 3000 (Thermo Fisher).

Techniques: Imaging, Western Blot, Variant Assay, Immunostaining, Incubation, Staining, Lysis, Activity Assay, Labeling, Flow Cytometry

Journal: Oncotarget

Article Title: Immunotherapy of ovarian cancer with a monoclonal antibody specific for the extracellular domain of anti-Müllerian hormone receptor II

doi: 10.18632/oncotarget.27585

Figure Lengend Snippet: Human EOC tumors were injected s. c. into immunodeficient mice. When tumors became palpable, mice were injected i. p. with 200 μg of either the 4D12G1 mAb or an isotype control mAb weekly for 5 continuous weeks. Treatment with the 4D12G1 mAb significantly inhibited the growth of OVCAR8 tumors in ( A ) severely immunodeficient NSG mice ( P < 0.001) and in ( B ) T cell-deficient athymic nude mice ( P < 0.0001). More importantly, treatment with the 4D12G1 mAb significantly inhibited the growth of three primary HGSOC tumors ( P < 0.0001 in all cases) generated from recently diagnosed patients and xenografted into immunodeficient NSG mice including ( C ) PDX-4, ( D ) PDX-6, and ( E ) PDX-9. ( F ) Detection of caspase-3 positive cells in the OVCAR8 (upper row) and PDX-4 tumors (lower row) from NSG mice at 20× is shown by arrows in mice treated with the 4D12G1 mAb (right column) compared to mice treated with isotype control mAb (left column). Caspase-3 data shown are representative of three experiments yielding similar results. All error bars indicate ± SD.

Article Snippet: The AMHR2-OVCAR8 cell line was generated by cloning transcript variant 1 of human AMHR2 (Accession #NM_020547; Origene, Rockville, MD) into pEZ-M68 vector (GeneCopoeia, Rockville, MD) and using this expression vector to transfect OVCAR8 cells using Lipofectamine™ 3000 (Thermo Fisher).

Techniques: Injection, Generated

Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: OVCAR-8 viability after a) 24 hours and b) 72 hours incubation with different concentrations of PTX-NCs, Si[PTX-NC]s and Taxol.

Article Snippet: 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Incubation

Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: a) NSCs migration after loading with Si[PTX-NC]s, PTX-NCs and Abraxane by Boyden chamber assay; b) OVCAR-8 viability after co-culture with different ratios of PTX formulations by luciferase luminescence assay. c) OVCAR-8 viability after 96 hours of treatments with equivalent concentration of PTX-NC, Si[PTX-NC] and NSC/Si[PTX-NC].

Article Snippet: 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Migration, Boyden Chamber Assay, Co-Culture Assay, Luciferase, Luminescence Assay, Concentration Assay

Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: Luminescent imaging of mice (A) before and (B) after treatment with (a) PTX-NC, (b) Si[PTX-NC], (c) NSC/Si[PTX-NC], (d) NSC, (e) PBS, (f) 2 first mice are from the PTX-NC and third mouse is from Si[PTX-NC], imaged 2 days earlier than the rest of mice. (C) Quantified analysis of images represented as a percentage of intensity after each week of treatment (Week 1, week2, week3 ) relative to the pre-treatment (Week0), (D) total tumor weight collected after sacrificing mice. Dose of PTX injected is 0.5 mg/kg for all PTX treatment groups. The NSC number in NSC group is matched to the Number in NSC/Si[PTX-NC]. All Treatments here started 3 weeks after IP injection of 2.10E6 OVCAR-8 cells, and was performed for 3 weeks, 2 times a week. After the pre-treatment imaging (in A), all mice were repartitioned into groups based on the mean intensities (with exclusion of mice with low to no signal) and then treated randomly with the mentioned treatments.

Article Snippet: 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Imaging, Injection

Journal: The EMBO Journal

Article Title: Inhibitors of eIF1A-ribosome interaction unveil uORF-dependent regulation of translation initiation and antitumor and antiviral effects

doi: 10.1038/s44318-025-00449-6

Figure Lengend Snippet: ( A ) Gene and pathways enrichment analysis of 1Ai-3638 and 1Ai-8214 downregulated genes by the Gene Analytics tool. ( B ) OVCAR8 ovarian carcinoma cell line was incubated with increasing concentrations of 1Ais for 72 h, and then cell viability was assessed. Viability (100%) is normalized to 0 concentration (DMSO), n = 3 independent biological replicates, and error bars represent SD. ( C ) 1Ais toxicity assessment. Mice weight was measured at different time points after 1Ai-3638 and 1Ai-8214 subcutaneous injection for 3 weeks (assessed every 3–4 days, 3 mice per concentration, 21 mice in total) ( D ) OVCAR8 cell-derived xenografts weight after 1Ais treatment (10 mice per group). OVCAR8 cells were injected into the ovaries of immunocompromised female mice. Out of ten mice in each group (1Ai-3638,1Ai-8214 and DMSO) nine were included as they presented an ovarian tumor. 1Ais were injected into the mice’s peritoneal cavity five times a week for 4 weeks. Finally, mice were sacrificed, and tumor weight was measured. Results are presented as boxplots in which the center = median, box ends = interquartile range (IQR), bottom whisker = minimum and upper whisker= maximum, n = 9. Weight monitoring of mice during the experiment is presented in Appendix Fig. S . * P < 0.05, ** P < 0.01, Student t test. .

Article Snippet: OVCAR8 , Creative Biolabs , IOC-ZP305.

Techniques: Incubation, Concentration Assay, Injection, Derivative Assay, Whisker Assay

Journal: Nanomaterials

Article Title: Safety Evaluations of Rapamycin Perfluorocarbon Nanoparticles in Ovarian Tumor-Bearing Mice

doi: 10.3390/nano14211752

Figure Lengend Snippet: Rapamycin PFC nanoparticles inhibit tumoral angiogenesis without affecting normal vessels, illustrated by CD31 staining (Red). ( A , B ). Tumor from control group ( A ) and the rapamycin PFC nanoparticles-treated group ( B ) (magnification: 20×, scale bar: 50 µm) (Green: Human ovarian tumor cell, OVCAR8, engineered with stable GFP overexpression). ( C , D ). Heart from control group ( C ) and the rapamycin PFC nanoparticles-treated group ( D ) (magnification: 20×, scale bar: 50 µm). ( E , F ). Renal cortex from control group ( E ) and the rapamycin PFC nanoparticles-treated group ( F ) (magnification: 40×, scale bar: 20 µm). ( G , H ). Renal medulla from control group ( G ) and the rapamycin PFC nanoparticles-treated group ( H ) (magnification: 40×, scale bar: 20 µm). ( I , J ). Liver from control group ( I ) and the rapamycin PFC nanoparticles-treated group ( J ) (magnification: 20×, scale bar: 50 µm). Blue: DAPI staining.

Article Snippet: Human ovarian tumor cells, OVCAR8 engineered with stable GFP overexpression (CSC-RR0468, www.creative-biogene.com (accessed on 16 November 2021)), were seeded at 100,000 cells in a Delta T Dish 0.17 mm (clear) (Cat#: 04200417C-50, Bioptechs Inc., Butler, PA, USA).

Techniques: Staining, Control, Over Expression

Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: OVCAR-8 viability after a) 24 hours and b) 72 hours incubation with different concentrations of PTX-NCs, Si[PTX-NC]s and Taxol.

Article Snippet: In Vivo Orthotopic Ovarian Cancer Model, bioluminescent imaging and tumor collection 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Incubation

Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: a) NSCs migration after loading with Si[PTX-NC]s, PTX-NCs and Abraxane by Boyden chamber assay; b) OVCAR-8 viability after co-culture with different ratios of PTX formulations by luciferase luminescence assay. c) OVCAR-8 viability after 96 hours of treatments with equivalent concentration of PTX-NC, Si[PTX-NC] and NSC/Si[PTX-NC].

Article Snippet: In Vivo Orthotopic Ovarian Cancer Model, bioluminescent imaging and tumor collection 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Migration, Boyden Chamber Assay, Co-Culture Assay, Luciferase, Luminescence Assay, Concentration Assay

Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: Luminescent imaging of mice (A) before and (B) after treatment with (a) PTX-NC, (b) Si[PTX-NC], (c) NSC/Si[PTX-NC], (d) NSC, (e) PBS, (f) 2 first mice are from the PTX-NC and third mouse is from Si[PTX-NC], imaged 2 days earlier than the rest of mice. (C) Quantified analysis of images represented as a percentage of intensity after each week of treatment (Week 1, week2, week3 ) relative to the pre-treatment (Week0), (D) total tumor weight collected after sacrificing mice. Dose of PTX injected is 0.5 mg/kg for all PTX treatment groups. The NSC number in NSC group is matched to the Number in NSC/Si[PTX-NC]. All Treatments here started 3 weeks after IP injection of 2.10E6 OVCAR-8 cells, and was performed for 3 weeks, 2 times a week. After the pre-treatment imaging (in A), all mice were repartitioned into groups based on the mean intensities (with exclusion of mice with low to no signal) and then treated randomly with the mentioned treatments.

Article Snippet: In Vivo Orthotopic Ovarian Cancer Model, bioluminescent imaging and tumor collection 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Imaging, Injection