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  • 99
    New England Biolabs bst dna polymerase
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Bst Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1634 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher calf intestinal phosphatase
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Calf Intestinal Phosphatase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Eiken Chemical outer primers f3
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Outer Primers F3, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Bio-Serv outer primers f3
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Outer Primers F3, supplied by Bio-Serv, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Warner Instruments borosilicate glass pipettes
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Borosilicate Glass Pipettes, supplied by Warner Instruments, used in various techniques. Bioz Stars score: 92/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher amv reverse transcriptase
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Amv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2381 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Premier Biosoft lamp designer 1 10
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Lamp Designer 1 10, supplied by Premier Biosoft, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore betaine
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Betaine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Premier Biosoft licensed lamp designer 1 15 software
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Licensed Lamp Designer 1 15 Software, supplied by Premier Biosoft, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Eiken Chemical primerexplorer v4 software
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Primerexplorer V4 Software, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 88/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs bst 2 0 dna polymerase
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Bst 2 0 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Eiken Chemical primer explorer v5
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Primer Explorer V5, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Eiken Chemical primer explorer
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Primer Explorer, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sutter Instrument p 97 flaming brown micropipette puller
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    P 97 Flaming Brown Micropipette Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 92/100, based on 828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dntps
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Dntps, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4077 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Avantor primer explorer version 4 0
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Primer Explorer Version 4 0, supplied by Avantor, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen plasmid mini kit
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Plasmid Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4733 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs bst dna polymerase large fragment
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Bst Dna Polymerase Large Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa dntps
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Dntps, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14041 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    OptiGene Ltd isothermal master mix
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Isothermal Master Mix, supplied by OptiGene Ltd, used in various techniques. Bioz Stars score: 89/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bruker Corporation advance iii hd 400 spectrometer
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Advance Iii Hd 400 Spectrometer, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Eiken Chemical loopamp rna amplification kit
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Loopamp Rna Amplification Kit, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 91/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs thermopol buffer
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Thermopol Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa amv reverse transcriptase
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Amv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Eiken Chemical lamp primer
    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of <t>betaine.</t> Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.
    Lamp Primer, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 89/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the Bst DNA polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6

    Journal: Scientific Reports

    Article Title: Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis

    doi: 10.1038/s41598-017-13881-4

    Figure Lengend Snippet: Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the Bst DNA polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6

    Article Snippet: The quantity of Bst DNA polymerase in the reaction mixture was set at 2.0 U, 4.0 U, 6.0 U and 8.0 U. Mg2+ concentrations were set to 5.0 mM, 6.0 mM, 7.0 mM and 8.0 mM, respectively (Fig. ).

    Techniques: Amplification

    The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of betaine. Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.

    Journal: BMC Infectious Diseases

    Article Title: Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71

    doi: 10.1186/1471-2334-11-197

    Figure Lengend Snippet: The optimal condition of RT-LAMP for the detection of EV71 . (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of betaine. Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.

    Article Snippet: Reaction system of RT-LAMP The RT-LAMP reaction was performed in a 25 μl of reaction mixture containing 0.8 μM (each) FIP and BIP inner primers, 0.2 μM forward outer primer (F3) and backward outer primer (B3), 0.4 μM LF and LB (each), 0.4 mM deoxynucleoside triphosphates (dNTPs), 1 M betaine (Sigma, USA), 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 2.8 mM MgSO4 , 0.1% Triton X-100, 0.5 μl of 8 U Bst DNA polymerase (New England Biolabs, USA), 40 U of reverse transcriptase (Invitrogen), and 5 μl of target RNA.

    Techniques: RT Lamp Assay, Agarose Gel Electrophoresis, Marker, Concentration Assay