Journal: Hepatology communications

Article Title: Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy.

doi: 10.1002/hep4.1234

Figure Lengend Snippet: FIG. 2. Images of BM biopsy in Cirrhosis and Control cases. (A) Panel I. Representative Images of cirrhosis and control BM (left) showing the osteoblast cells and bar graph (right) showing number of osteoblast (mean ± SE) in cirrhosis and control BM (H&E). Panel II. Cirrhosis and control BM (left) showing the osteocytes and bar graph (right) showing number of osteocytes (mean ± SE) in cirrhosis and control BM (H&E). Panel III. Alcian blue-stained chondroblasts in cirrhosis and control BM biopsy section (left) and bar graph (right) showing number of chondroblasts (mean ± SE) in cirrhosis and control BM. (B) Panel I. Representing osteocalcin+ cells (mean ± SE) in cirrhosis and control BM biopsy section (left) and bar graph (right) showing number of osteocalcin+ cells in cirrhosis and control BM. Panel II. Osteocalcin+ area (mean ± SE) in cirrhosis and control BM biopsy section (left) and bar graph (right) showing percentage osteocalcin+ area in cirrhosis and control BM. Panel III. Showing osteonectin+ cells (mean ± SE) in cirrhosis and control BM biopsy section (left) and bar graph (right) showing osteonectin in cirrhosis and control BM (C) Nestin+ BM MSCs in cirrhosis and control BM biopsy section (left) and bar graph (right) showing number of these cells in cirrhosis and control BM. (D) CD169+ BM macrophage (mean ± SE) in cirrhosis and control BM biopsy section (left) and bar graph (right) showing number of CD169+ cells in cirrhosis and control BM. (All image magnification ×200, area 1.3 mm2). Abbreviations: BM, bone marrow; H&E, hematoxylin and eosin.

Article Snippet: Osteocalcin- and osterix+ cell counting were done using Image J software (summarized in the Supporting Material). enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay for osteocalcin (ABIN368354; N-MID, Aachen, Germany), osteonectin (E-EL-H1351; Elabscience), bone morphogenetic protein 2 (BMP2) (E13650313; Sincere Biotech, Beijing, China), fibroblast growth factor 23 (FGF23) (E13652282; Sincere Biotech), and dipeptidyl peptidase 4 (DPP4) (E-EL-H0058; Elabscience) was performed on BM plasma and peripheral blood plasma of cirrhosis and control cases. skeletal survey A baseline bone survey for bone loss was done by a DEXA scan of the lumbar spine and femoral neck in cases of cirrhosis and was classified as described. (6) statistical analysis Statistical analysis was carried out using Statistical Package for Social Sciences (SPSS, version 22.0; IBM Corp, Armonk, NY).

Techniques: Control, Staining

Journal: Hepatology communications

Article Title: Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy.

doi: 10.1002/hep4.1234

Figure Lengend Snippet: FIG. 4. Graphs showing BM plasma (left) and peripheral blood plasma (right) of the same patients displaying levels of bone-building proteins. (A) Osteocalcin, (B) osteonectin, (C) bone morphogenic protein, and (D) anti-bone building proteins FGF23 and (E) DPP4 in control and patients with cirrhosis with Child-Turcotte-Pugh (CTP) A, B, or C { median (interquartile range) }.

Article Snippet: Osteocalcin- and osterix+ cell counting were done using Image J software (summarized in the Supporting Material). enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay for osteocalcin (ABIN368354; N-MID, Aachen, Germany), osteonectin (E-EL-H1351; Elabscience), bone morphogenetic protein 2 (BMP2) (E13650313; Sincere Biotech, Beijing, China), fibroblast growth factor 23 (FGF23) (E13652282; Sincere Biotech), and dipeptidyl peptidase 4 (DPP4) (E-EL-H0058; Elabscience) was performed on BM plasma and peripheral blood plasma of cirrhosis and control cases. skeletal survey A baseline bone survey for bone loss was done by a DEXA scan of the lumbar spine and femoral neck in cases of cirrhosis and was classified as described. (6) statistical analysis Statistical analysis was carried out using Statistical Package for Social Sciences (SPSS, version 22.0; IBM Corp, Armonk, NY).

Techniques: Clinical Proteomics, Control

Journal: Hepatology communications

Article Title: Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy.

doi: 10.1002/hep4.1234

Figure Lengend Snippet: FIG. 5. Distribution graphs. (A) Panel I. Osteoblasts. Panel II. Osteocytes. Panel III. Chondroblasts. (B) Panel I. Osteocalcin+ cells. Panel II. Percentage osteocalcin+ area. Panel III. Osteonectin+ cells. (C) Nestin+ MSCs. (D) CD169+ BM macrophages (all expressed in mean ± SE) in BM sections of patients with cirrhosis with normal bone density, osteopenia, and osteoporosis; evaluated by T score on bone densitometry scan.

Article Snippet: Osteocalcin- and osterix+ cell counting were done using Image J software (summarized in the Supporting Material). enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay for osteocalcin (ABIN368354; N-MID, Aachen, Germany), osteonectin (E-EL-H1351; Elabscience), bone morphogenetic protein 2 (BMP2) (E13650313; Sincere Biotech, Beijing, China), fibroblast growth factor 23 (FGF23) (E13652282; Sincere Biotech), and dipeptidyl peptidase 4 (DPP4) (E-EL-H0058; Elabscience) was performed on BM plasma and peripheral blood plasma of cirrhosis and control cases. skeletal survey A baseline bone survey for bone loss was done by a DEXA scan of the lumbar spine and femoral neck in cases of cirrhosis and was classified as described. (6) statistical analysis Statistical analysis was carried out using Statistical Package for Social Sciences (SPSS, version 22.0; IBM Corp, Armonk, NY).

Techniques:

Journal: The Journal of International Medical Research

Article Title: Osteonectin as a screening marker for pancreatic cancer: A prospective study

doi: 10.1177/0300060518772413

Figure Lengend Snippet: Osteonectin serum concentrations in ng/mL in patients with cancer, patients with acute pancreatitis, and healthy individuals

Article Snippet: Human osteonectin was detected by a quantitative sandwich enzyme immunoassay technique (Human Osteonectin ELISA kit; Cusabio, College Park, MD, USA) according to the manufacturer’s instructions.

Techniques:

Journal: The Journal of International Medical Research

Article Title: Osteonectin as a screening marker for pancreatic cancer: A prospective study

doi: 10.1177/0300060518772413

Figure Lengend Snippet: Box plot by group shows the median, 25th to 75th interquartile range, and minimum to maximum serum osteonectin concentrations in ng/mL.

Article Snippet: Human osteonectin was detected by a quantitative sandwich enzyme immunoassay technique (Human Osteonectin ELISA kit; Cusabio, College Park, MD, USA) according to the manufacturer’s instructions.

Techniques:

Journal: The Journal of International Medical Research

Article Title: Osteonectin as a screening marker for pancreatic cancer: A prospective study

doi: 10.1177/0300060518772413

Figure Lengend Snippet: Receiver operating characteristic curve. Serum osteonectin concentrations of >100.18 ng/mL at enrolment had 86% accuracy in predicting pancreatic cancer.

Article Snippet: Human osteonectin was detected by a quantitative sandwich enzyme immunoassay technique (Human Osteonectin ELISA kit; Cusabio, College Park, MD, USA) according to the manufacturer’s instructions.

Techniques:

Journal: The Journal of International Medical Research

Article Title: Osteonectin as a screening marker for pancreatic cancer: A prospective study

doi: 10.1177/0300060518772413

Figure Lengend Snippet: Least-square means and 95% confidence intervals of serum osteonectin concentrations in ng/mL among the three groups

Article Snippet: Human osteonectin was detected by a quantitative sandwich enzyme immunoassay technique (Human Osteonectin ELISA kit; Cusabio, College Park, MD, USA) according to the manufacturer’s instructions.

Techniques: