Journal: Frontiers in Toxicology
Article Title: Optimisation of an in vitro human cardiovascular model on-a-chip for toxicological assessment of nicotine delivery products
doi: 10.3389/ftox.2024.1395670
Figure Lengend Snippet: (A) . Image and representative diagram of the 2-lane OrganoPlate. Boxes 1, 2 and 4 mark where reagents/cells can be added to the respective parts of the chip. x and y -axes show the chip orientation on the plate; (B) . Rocker with plate and diagrammatic representation of the direction and gradient of media flow; (C) . Diagrammatic representation of the formation of the cell layer following seeding of the human coronary artery endothelial cells (HCAECs) (cells shown in white) within the 2-lane OrganoPlate. Red areas represent the perfusion channel, blue areas the extracellular matrix (ECM) and grey the plate PhaseGuide. Bidirectional arrows represent the direction of flow and x and y -axes the orientation; g = gravity. (D) . Diagrammatic representation of the cell culture within the OrganoPlate 2-lane. The arrow represents the bidirectional flow of medium/suspension cells; green circles represent THP-1 cells; the endothelial cell vessel is represented in red and the extracellular collagen matrix in blue; (E) . Image of the 3D HCAEC vessel stained with CD31.
Article Snippet: Human Coronary Artery Endothelial Cells (HCAECs) were cultured on an OrganoPlate ® 2-lane chip (Mimetas BV) combined with THP-1 monocytes under flow conditions.
Techniques: Cell Culture, Suspension, Staining