Journal: Molecular Vision

Article Title: Gene and noncoding RNA regulation underlying photoreceptor protection: microarray study of dietary antioxidant saffron and photobiomodulation in rat retina

doi:

Figure Lengend Snippet: TaqMan Probes used for qPCR

Article Snippet: optineurin , Optn , Rn00595346_m1.

Techniques: TaqMan Assay, Control, Binding Assay

Journal: Molecular Vision

Article Title: Gene and noncoding RNA regulation underlying photoreceptor protection: microarray study of dietary antioxidant saffron and photobiomodulation in rat retina

doi:

Figure Lengend Snippet: Genes and ncRNA regulated by photobiomodulation and saffron during light damage but not regulated by light damage alone.

Article Snippet: optineurin , Optn , Rn00595346_m1.

Techniques: Binding Assay, Ubiquitin Proteomics

Journal: Autophagy

Article Title: Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis

doi: 10.1080/15548627.2023.2169309

Figure Lengend Snippet: MYH10 recruits autophagic receptor proteins to autophagosomes. (A) Co-immunoprecipitation analysis of MYH10 and autophagic receptors. HEK293T cells were transfected with vectors expressing proteins tagged with Flag-MYH10 and green fluorescent proteins (GFP, GFP-OPTN, GFP-CALCOCO2, or GFP-SQSTM1), and were subjected to co-immunoprecipitation with anti-Flag agarose beads in the absence or presence of amino acid starvation for 2 h. Western blotting was done with anti-Flag antibody or anti-GFP antibody. (B) Cellular localization of GFP-MYH10 with monomeric red fluorescent protein (mRFP)-autophagic receptors (OPTN, CALCOCO2, and SQSTM1). U2OS cells stably expressing GFP-MYH10 were transiently transfected with mRFP-OPTN, -CALCOCO2, or -SQSTM1. Scale bar: 20 μm. (C and F) Cells stably expressing YFP-PRKN were stained with MitoTracker (red) and antibodies against SQSTM1 (C) or OPTN (F) and treated with carbonyl cyanide m-chlorophenyl hydrazone (CCCP, 20 μM) and bafilomycin A 1 (BafA1, 100 nM) for 2 h. White arrowheads indicate PRKN-positive and SQSTM1- (C) or OPTN-negative (F) mitochondria. Scale bar: 20 μm. (D-H) Number and relative percentage of PRKN- and SQSTM1- (D and E) or OPTN-positive (G and H) mitophagosomes, which was calculated as the number of SQSTM1- or OPTN-positive mitophagosomes divided by the total number of PRKN-positive damaged mitochondria. Values are mean ± SEM of n >15 randomly selected cells. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by paired two-tailed t test.

Article Snippet: GFP-OPTN , Sino Biological , HG14478-ANG.

Techniques: Immunoprecipitation, Transfection, Expressing, Western Blot, Stable Transfection, Staining, Two Tailed Test

Journal: Autophagy

Article Title: Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis

doi: 10.1080/15548627.2023.2169309

Figure Lengend Snippet: Reagents and resources used in this study.

Article Snippet: GFP-OPTN , Sino Biological , HG14478-ANG.

Techniques: Protease Inhibitor, Electron Microscopy, Silver Staining, Plasmid Preparation, Negative Control, Subcloning, Recombinant, Expressing, shRNA