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  • 99
    Thermo Fisher optimem
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21807 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem/product/Thermo Fisher
    Average 99 stars, based on 21807 article reviews
    Price from $9.99 to $1999.99
    optimem - by Bioz Stars, 2020-11
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    91
    Thermo Fisher serum free optimem
    Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free <t>Optimem</t> +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.
    Serum Free Optimem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum free optimem/product/Thermo Fisher
    Average 91 stars, based on 901 article reviews
    Price from $9.99 to $1999.99
    serum free optimem - by Bioz Stars, 2020-11
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    99
    Thermo Fisher optimem reduced serum medium
    Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free <t>Optimem</t> +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.
    Optimem Reduced Serum Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem reduced serum medium/product/Thermo Fisher
    Average 99 stars, based on 600 article reviews
    Price from $9.99 to $1999.99
    optimem reduced serum medium - by Bioz Stars, 2020-11
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    88
    Thermo Fisher antibiotic free optimem
    Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free <t>Optimem</t> +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.
    Antibiotic Free Optimem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibiotic free optimem/product/Thermo Fisher
    Average 88 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    antibiotic free optimem - by Bioz Stars, 2020-11
    88/100 stars
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    99
    Thermo Fisher optimem glutamax
    Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free <t>Optimem</t> +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.
    Optimem Glutamax, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem glutamax/product/Thermo Fisher
    Average 99 stars, based on 159 article reviews
    Price from $9.99 to $1999.99
    optimem glutamax - by Bioz Stars, 2020-11
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    99
    Thermo Fisher optimem gibco medium
    Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free <t>Optimem</t> +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.
    Optimem Gibco Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem gibco medium/product/Thermo Fisher
    Average 99 stars, based on 156 article reviews
    Price from $9.99 to $1999.99
    optimem gibco medium - by Bioz Stars, 2020-11
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    92
    Mirus Bio optimem
    Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free <t>Optimem</t> +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.
    Optimem, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 92/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem/product/Mirus Bio
    Average 92 stars, based on 108 article reviews
    Price from $9.99 to $1999.99
    optimem - by Bioz Stars, 2020-11
    92/100 stars
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    92
    Horizon Discovery optimem
    Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free <t>Optimem</t> +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.
    Optimem, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 92/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem/product/Horizon Discovery
    Average 92 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    optimem - by Bioz Stars, 2020-11
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    Image Search Results


    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in OptiMEM medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.

    Journal: Autophagy

    Article Title: Using LC3 to Monitor Autophagy Flux in the Retinal Pigment Epithelium

    doi:

    Figure Lengend Snippet: Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in OptiMEM medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.

    Article Snippet: Preparation of the transfection solution is as follows: Per well, dilute 0.3 μg GFP-LC3 plasmid DNA in 25 μl OptiMEM medium (no serum/antibiotics) in a sterile polystyrene tube and dilute 0.8 μl Lipofectamine 2000 (Invitrogen, 11668027) in 25 μl OptiMEM (no serum/antibiotics) into a second sterile polystyrene tube and incubate for 5 min at room temperature.

    Techniques: Labeling, Transfection, Plasmid Preparation, Marker

    Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free Optimem +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.

    Journal: PLoS ONE

    Article Title: Kinetics of Leptin Binding to the Q223R Leptin Receptor

    doi: 10.1371/journal.pone.0094843

    Figure Lengend Snippet: Expression and isolation of recombinant murine LepR ec -Fc fusion proteins. a ) Expression of murine LepR ec -Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepR ec -Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1 : Mock transfected at 48 hours. Lane 2 : WT transfected at 24 hours. Lane 3 : WT transfected at 48 hours. Lane 4 : Q223R transfected at 24 hours. Lane 5 : Q223R transfected at 48 hours. Lane 6 : Mock transfected at 48 hours. Lane 7 : WT transfected at 48 hours. Lane 8 : Q223R transfected at 48 hours. b ) Concentration and buffer exchange of murine LepR ec -Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free Optimem +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with α-murine IgG1 specific to the Fc region (bottom). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R supernatant. Lane 6 : Q223R Concentrate. Lane 7 : Q223R Filtrate. c ) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with α-murine leptin receptor (R D scientific). Lane 1 : Expression medium. Lane 2 : WT supernatant. Lane 3 : WT concentrate. Lane 4 : WT filtrate. Lane 5 : Q223R Filtrate. Lane 6 : Q223R Concentrate. Lane 7 : Q223R supernatant.

    Article Snippet: 12 hours after transfection, monolayers were washed and growth medium was replaced with serum free Optimem (Gibco) supplemented with 2 mM sodium butyrate.

    Techniques: Expressing, Isolation, Recombinant, Construct, Transfection, SDS Page, Western Blot, Concentration Assay, Buffer Exchange, Staining