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  • 99
    Thermo Fisher opti mem
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Opti Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 61036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opti mem/product/Thermo Fisher
    Average 99 stars, based on 61036 article reviews
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    99
    Thermo Fisher optimem
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21004 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem/product/Thermo Fisher
    Average 99 stars, based on 21004 article reviews
    Price from $9.99 to $1999.99
    optimem - by Bioz Stars, 2020-10
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    99
    Thermo Fisher opti mem reduced serum medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Opti Mem Reduced Serum Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opti mem reduced serum medium/product/Thermo Fisher
    Average 99 stars, based on 4884 article reviews
    Price from $9.99 to $1999.99
    opti mem reduced serum medium - by Bioz Stars, 2020-10
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    91
    Thermo Fisher serum free opti mem medium
    Secretion of different B4GalT4 versions. HEK293T cells stably overexpressing variants of B4GalT4 were cultured in <t>Opti-MEM</t> without FBS for 24 h. Conditioned media were concentrated by centrifugation in Amicon Pro 10 kDa cut-off membranes and analyzed by Western blotting with anti-B4GalT4 and anti-Mgat5 antibodies for protein assessment and loading control, respectively
    Serum Free Opti Mem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1016 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum free opti mem medium/product/Thermo Fisher
    Average 91 stars, based on 1016 article reviews
    Price from $9.99 to $1999.99
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    91
    Thermo Fisher serum free optimem
    Analyses of cell viability, cytotoxicity and caspase-3/7 activity after treatment of EJ28 cells with siRNAs and subsequent chemotherapy (CT). Cells were transfected with the respective siRNAs for four hours. “CT only” cells were treated with serum-free <t>OptiMEM</t> medium during transfection. Twenty-four hours after transfection start, cells were treated with 0.9 μg/mL mitomycin C for two hours. ApoTox-Glo triplex assay was performed 72 h after transfection. Values shown are mean values of duplicates and are relative to the control siRNA “ns-si” plus CT treatment (=100%).
    Serum Free Optimem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 887 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher opti mem low serum media
    Transient expression of vinculin partially rescues Certhrax-induced cell detachment. HeLa cells were transfected with pEGFP, pEGFP-vinculin, or pEGFP-paxillin ± pCerADPr. 20 h HPT, cells were washed 4 times in <t>Opti-MEM</t> and color micrographs taken.
    Opti Mem Low Serum Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in OptiMEM medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.

    Journal: Autophagy

    Article Title: Using LC3 to Monitor Autophagy Flux in the Retinal Pigment Epithelium

    doi:

    Figure Lengend Snippet: Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in OptiMEM medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.

    Article Snippet: Preparation of the transfection solution is as follows: Per well, dilute 0.3 μg GFP-LC3 plasmid DNA in 25 μl OptiMEM medium (no serum/antibiotics) in a sterile polystyrene tube and dilute 0.8 μl Lipofectamine 2000 (Invitrogen, 11668027) in 25 μl OptiMEM (no serum/antibiotics) into a second sterile polystyrene tube and incubate for 5 min at room temperature.

    Techniques: Labeling, Transfection, Plasmid Preparation, Marker

    Secretion of different B4GalT4 versions. HEK293T cells stably overexpressing variants of B4GalT4 were cultured in Opti-MEM without FBS for 24 h. Conditioned media were concentrated by centrifugation in Amicon Pro 10 kDa cut-off membranes and analyzed by Western blotting with anti-B4GalT4 and anti-Mgat5 antibodies for protein assessment and loading control, respectively

    Journal: Glycoconjugate Journal

    Article Title: N-glycosylation of the human β1,4-galactosyltransferase 4 is crucial for its activity and Golgi localization

    doi: 10.1007/s10719-020-09941-z

    Figure Lengend Snippet: Secretion of different B4GalT4 versions. HEK293T cells stably overexpressing variants of B4GalT4 were cultured in Opti-MEM without FBS for 24 h. Conditioned media were concentrated by centrifugation in Amicon Pro 10 kDa cut-off membranes and analyzed by Western blotting with anti-B4GalT4 and anti-Mgat5 antibodies for protein assessment and loading control, respectively

    Article Snippet: 2–3 h before the measurement the conditioned medium was replaced with the serum-free OPTI-MEM medium (Life Technologies, CA, USA).

    Techniques: Stable Transfection, Cell Culture, Centrifugation, Western Blot

    Analyses of cell viability, cytotoxicity and caspase-3/7 activity after treatment of EJ28 cells with siRNAs and subsequent chemotherapy (CT). Cells were transfected with the respective siRNAs for four hours. “CT only” cells were treated with serum-free OptiMEM medium during transfection. Twenty-four hours after transfection start, cells were treated with 0.9 μg/mL mitomycin C for two hours. ApoTox-Glo triplex assay was performed 72 h after transfection. Values shown are mean values of duplicates and are relative to the control siRNA “ns-si” plus CT treatment (=100%).

    Journal: International Journal of Molecular Sciences

    Article Title: Enhanced Inhibition of Bladder Cancer Cell Growth by Simultaneous Knockdown of Antiapoptotic Bcl-xL and Survivin in Combination with Chemotherapy

    doi: 10.3390/ijms140612297

    Figure Lengend Snippet: Analyses of cell viability, cytotoxicity and caspase-3/7 activity after treatment of EJ28 cells with siRNAs and subsequent chemotherapy (CT). Cells were transfected with the respective siRNAs for four hours. “CT only” cells were treated with serum-free OptiMEM medium during transfection. Twenty-four hours after transfection start, cells were treated with 0.9 μg/mL mitomycin C for two hours. ApoTox-Glo triplex assay was performed 72 h after transfection. Values shown are mean values of duplicates and are relative to the control siRNA “ns-si” plus CT treatment (=100%).

    Article Snippet: Twenty-four or 72 h after seeding, cells were transfected for 4 h in serum-free OptiMEM (Life Technologies, Darmstadt, Germany) with a total of 40 nM siRNAs using DOTAP liposomal transfection reagent (ratio 1:30, w /w ) according to the manufacturer’s instructions (Roche, Mannheim, Germany).

    Techniques: Activity Assay, Transfection

    Apoptosis rate after treatment of J82 bladder cancer cells with siRNAs and chemotherapy (CT). Cells were transfected with the respective siRNAs for four hours. “CT only” cells were treated with serum-free OptiMEM medium during transfection. Twenty-four hours after transfection start, cells were treated with 1.2 μg/mL cisplatin for 24 h or with 1.0 μg/mL mitomycin C for two hours. Apoptosis rate—presented as sum of early and late apoptotic cells—was determined 72 h after transfection start. Values shown are averages of two independent experiments. Error bars represent the mean deviation.

    Journal: International Journal of Molecular Sciences

    Article Title: Enhanced Inhibition of Bladder Cancer Cell Growth by Simultaneous Knockdown of Antiapoptotic Bcl-xL and Survivin in Combination with Chemotherapy

    doi: 10.3390/ijms140612297

    Figure Lengend Snippet: Apoptosis rate after treatment of J82 bladder cancer cells with siRNAs and chemotherapy (CT). Cells were transfected with the respective siRNAs for four hours. “CT only” cells were treated with serum-free OptiMEM medium during transfection. Twenty-four hours after transfection start, cells were treated with 1.2 μg/mL cisplatin for 24 h or with 1.0 μg/mL mitomycin C for two hours. Apoptosis rate—presented as sum of early and late apoptotic cells—was determined 72 h after transfection start. Values shown are averages of two independent experiments. Error bars represent the mean deviation.

    Article Snippet: Twenty-four or 72 h after seeding, cells were transfected for 4 h in serum-free OptiMEM (Life Technologies, Darmstadt, Germany) with a total of 40 nM siRNAs using DOTAP liposomal transfection reagent (ratio 1:30, w /w ) according to the manufacturer’s instructions (Roche, Mannheim, Germany).

    Techniques: Transfection

    Induction of apoptosis and reduction in cell counts after combined siRNA plus chemotherapy (CT) treatments in EJ28 bladder cancer cells. Cells were transfected with the respective siRNAs for four hours. “CT only” cells were treated with serum-free OptiMEM medium during transfection. Twenty-four hours after transfection start, cells were treated with 2.1 μg/mL cisplatin for 24 h ( a , c ) or with 0.9 μg/mL mitomycin C for 2 h ( b , d ). Rate of apoptosis—presented as sum of early and late apoptotic cells—( a , b ), as well as cell counts ( c , d ) were determined 48 h and 72 h after transfection start.

    Journal: International Journal of Molecular Sciences

    Article Title: Enhanced Inhibition of Bladder Cancer Cell Growth by Simultaneous Knockdown of Antiapoptotic Bcl-xL and Survivin in Combination with Chemotherapy

    doi: 10.3390/ijms140612297

    Figure Lengend Snippet: Induction of apoptosis and reduction in cell counts after combined siRNA plus chemotherapy (CT) treatments in EJ28 bladder cancer cells. Cells were transfected with the respective siRNAs for four hours. “CT only” cells were treated with serum-free OptiMEM medium during transfection. Twenty-four hours after transfection start, cells were treated with 2.1 μg/mL cisplatin for 24 h ( a , c ) or with 0.9 μg/mL mitomycin C for 2 h ( b , d ). Rate of apoptosis—presented as sum of early and late apoptotic cells—( a , b ), as well as cell counts ( c , d ) were determined 48 h and 72 h after transfection start.

    Article Snippet: Twenty-four or 72 h after seeding, cells were transfected for 4 h in serum-free OptiMEM (Life Technologies, Darmstadt, Germany) with a total of 40 nM siRNAs using DOTAP liposomal transfection reagent (ratio 1:30, w /w ) according to the manufacturer’s instructions (Roche, Mannheim, Germany).

    Techniques: Transfection

    Transient expression of vinculin partially rescues Certhrax-induced cell detachment. HeLa cells were transfected with pEGFP, pEGFP-vinculin, or pEGFP-paxillin ± pCerADPr. 20 h HPT, cells were washed 4 times in Opti-MEM and color micrographs taken.

    Journal: The Journal of Biological Chemistry

    Article Title: Bacillus cereus Certhrax ADP-ribosylates Vinculin to Disrupt Focal Adhesion Complexes and Cell Adhesion *

    doi: 10.1074/jbc.M113.500710

    Figure Lengend Snippet: Transient expression of vinculin partially rescues Certhrax-induced cell detachment. HeLa cells were transfected with pEGFP, pEGFP-vinculin, or pEGFP-paxillin ± pCerADPr. 20 h HPT, cells were washed 4 times in Opti-MEM and color micrographs taken.

    Article Snippet: Unbound trypan blue was removed, cells were washed in Opti-MEM low serum media (Invitrogen), and color images were obtained using a ×10 objective and Retiga-2000R CCD camera (QImaging) with NIS Elements software (Nikon).

    Techniques: Expressing, Transfection