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  • 93
    R&D Systems opg
    Increased serum <t>OPG</t> levels in mice after microbial infection occurs via Fos family transcription factors. (A, B) Time-dependent elevation of OPG and <t>IFN-β</t> in serum, and colony forming units (CFU) in blood and spleen of 6-week-old C57BL/6J mice infected with Salmonella enterica χ3306 (A, n = 4 each point) or Staphylococcus aureus 92–1191 (B, n = 4 each point). “d” indicates death of one mouse. “ddd” indicates death of three mice. (C, D) Time-dependent elevation of OPG and IFN-β levels in serum after Mycobacterium (C, n = 4 each point) and influenza virus (D, n = 3 each point) infection of 6-week-old C57BL/6J mice. * P
    Opg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opg/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    opg - by Bioz Stars, 2021-09
    93/100 stars
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    opg  (Abcam)
    94
    Abcam opg
    Blocking of autophagy inhibits wear particle induced osteoclastogenesis in vitro. (a) The expression of <t>TRAP,</t> RANKL, and <t>OPG</t> was detected by real‐time PCR in RAW 264.7 cells under the indicated treatments. (b) RAW 264.7 cells were transfected with GFP‐LC3 for 24 h and then treated with Ti in the presence or absence of 3‐MA for 48 h to observe the punctuate GFP‐LC3 distribution by fluorescence microscopy; the expression of TRAP was also detected by immunofluorescence staining. (c) The cell culture supernatants in different groups were collected to detect RANKL and OPG with ELISA. (d) The expression of TRAP in RAW 264.7 cells in different groups was detected by western blotting. (e) Ti or 3‐MA treatment induced autophagy was detected by electron microscopy of bone sections. Data represent mean ± SEM; n = 5 (* p
    Opg, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opg/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    opg - by Bioz Stars, 2021-09
    94/100 stars
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    95
    BioVendor Instruments serum opg
    Blocking of autophagy inhibits wear particle induced osteoclastogenesis in vitro. (a) The expression of <t>TRAP,</t> RANKL, and <t>OPG</t> was detected by real‐time PCR in RAW 264.7 cells under the indicated treatments. (b) RAW 264.7 cells were transfected with GFP‐LC3 for 24 h and then treated with Ti in the presence or absence of 3‐MA for 48 h to observe the punctuate GFP‐LC3 distribution by fluorescence microscopy; the expression of TRAP was also detected by immunofluorescence staining. (c) The cell culture supernatants in different groups were collected to detect RANKL and OPG with ELISA. (d) The expression of TRAP in RAW 264.7 cells in different groups was detected by western blotting. (e) Ti or 3‐MA treatment induced autophagy was detected by electron microscopy of bone sections. Data represent mean ± SEM; n = 5 (* p
    Serum Opg, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum opg/product/BioVendor Instruments
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    serum opg - by Bioz Stars, 2021-09
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    86
    Santa Cruz Biotechnology opg
    Blocking of autophagy inhibits wear particle induced osteoclastogenesis in vitro. (a) The expression of <t>TRAP,</t> RANKL, and <t>OPG</t> was detected by real‐time PCR in RAW 264.7 cells under the indicated treatments. (b) RAW 264.7 cells were transfected with GFP‐LC3 for 24 h and then treated with Ti in the presence or absence of 3‐MA for 48 h to observe the punctuate GFP‐LC3 distribution by fluorescence microscopy; the expression of TRAP was also detected by immunofluorescence staining. (c) The cell culture supernatants in different groups were collected to detect RANKL and OPG with ELISA. (d) The expression of TRAP in RAW 264.7 cells in different groups was detected by western blotting. (e) Ti or 3‐MA treatment induced autophagy was detected by electron microscopy of bone sections. Data represent mean ± SEM; n = 5 (* p
    Opg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opg/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    opg - by Bioz Stars, 2021-09
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    Image Search Results


    Increased serum OPG levels in mice after microbial infection occurs via Fos family transcription factors. (A, B) Time-dependent elevation of OPG and IFN-β in serum, and colony forming units (CFU) in blood and spleen of 6-week-old C57BL/6J mice infected with Salmonella enterica χ3306 (A, n = 4 each point) or Staphylococcus aureus 92–1191 (B, n = 4 each point). “d” indicates death of one mouse. “ddd” indicates death of three mice. (C, D) Time-dependent elevation of OPG and IFN-β levels in serum after Mycobacterium (C, n = 4 each point) and influenza virus (D, n = 3 each point) infection of 6-week-old C57BL/6J mice. * P

    Journal: PLoS ONE

    Article Title: Osteoprotegerin Regulates Pancreatic β-Cell Homeostasis upon Microbial Invasion

    doi: 10.1371/journal.pone.0146544

    Figure Lengend Snippet: Increased serum OPG levels in mice after microbial infection occurs via Fos family transcription factors. (A, B) Time-dependent elevation of OPG and IFN-β in serum, and colony forming units (CFU) in blood and spleen of 6-week-old C57BL/6J mice infected with Salmonella enterica χ3306 (A, n = 4 each point) or Staphylococcus aureus 92–1191 (B, n = 4 each point). “d” indicates death of one mouse. “ddd” indicates death of three mice. (C, D) Time-dependent elevation of OPG and IFN-β levels in serum after Mycobacterium (C, n = 4 each point) and influenza virus (D, n = 3 each point) infection of 6-week-old C57BL/6J mice. * P

    Article Snippet: ELISA and biochemical tests Respective protein levels in mouse sera and culture media were evaluated using ELISA kits for OPG (R & D Systems), IFN-β (BD PharMingen), or insulin (Morinaga Institute of Biological Science).

    Techniques: Mouse Assay, Infection

    OPG expression in mouse β-cells and effect of OPG on glucose-stimulated insulin secretion. (A, B) Immunofluorescence analysis showing localization of OPG protein (red) with glucagon (green) (A) or insulin (green) (B) in pancreatic islets. OPG is predominantly expressed in β-cells based on co-localization with insulin. Scale bars, 20 μm. (C) Expression of Opg , Rank , and Rankl transcripts in isolated islets treated without or with LPS as measured by qPCR (n = 3–6). (D) Effect of LPS treatment on Opg , Rank and Rankl expression in MIN6 cells (n = 3). (E) Insulin secretion by MIN6 cells. Cells were untreated (n = 6) or treated with 100 ng/ml soluble RANKL (sRANKL, n = 6), 100 ng/ml recombinant OPG (rOPG, n = 6), 10 μg/ml LPS (n = 6), both LPS and sRANKL (n = 6), or both LPS and rOPG (n = 6), and then stimulated with 3, 9.8, or 20 mM glucose. Levels of secreted insulin were normalized to total cell protein. Shown are means ± SD. * P

    Journal: PLoS ONE

    Article Title: Osteoprotegerin Regulates Pancreatic β-Cell Homeostasis upon Microbial Invasion

    doi: 10.1371/journal.pone.0146544

    Figure Lengend Snippet: OPG expression in mouse β-cells and effect of OPG on glucose-stimulated insulin secretion. (A, B) Immunofluorescence analysis showing localization of OPG protein (red) with glucagon (green) (A) or insulin (green) (B) in pancreatic islets. OPG is predominantly expressed in β-cells based on co-localization with insulin. Scale bars, 20 μm. (C) Expression of Opg , Rank , and Rankl transcripts in isolated islets treated without or with LPS as measured by qPCR (n = 3–6). (D) Effect of LPS treatment on Opg , Rank and Rankl expression in MIN6 cells (n = 3). (E) Insulin secretion by MIN6 cells. Cells were untreated (n = 6) or treated with 100 ng/ml soluble RANKL (sRANKL, n = 6), 100 ng/ml recombinant OPG (rOPG, n = 6), 10 μg/ml LPS (n = 6), both LPS and sRANKL (n = 6), or both LPS and rOPG (n = 6), and then stimulated with 3, 9.8, or 20 mM glucose. Levels of secreted insulin were normalized to total cell protein. Shown are means ± SD. * P

    Article Snippet: ELISA and biochemical tests Respective protein levels in mouse sera and culture media were evaluated using ELISA kits for OPG (R & D Systems), IFN-β (BD PharMingen), or insulin (Morinaga Institute of Biological Science).

    Techniques: Expressing, Immunofluorescence, Isolation, Real-time Polymerase Chain Reaction, Recombinant

    OPG inhibits insulin secretion from β-cells under inflammatory conditions. When β-cells are exposed to inflammatory stimuli, they secrete OPG, which blocks RANKL-RANK signaling. Both osteoblast- and β-cell-derived OPG negatively regulates insulin secretion. Lower panel was adopted from Wei and Karsenty (2015) [ 45 ]. Glu, undercarboxylated. Gla, carboxylated.

    Journal: PLoS ONE

    Article Title: Osteoprotegerin Regulates Pancreatic β-Cell Homeostasis upon Microbial Invasion

    doi: 10.1371/journal.pone.0146544

    Figure Lengend Snippet: OPG inhibits insulin secretion from β-cells under inflammatory conditions. When β-cells are exposed to inflammatory stimuli, they secrete OPG, which blocks RANKL-RANK signaling. Both osteoblast- and β-cell-derived OPG negatively regulates insulin secretion. Lower panel was adopted from Wei and Karsenty (2015) [ 45 ]. Glu, undercarboxylated. Gla, carboxylated.

    Article Snippet: ELISA and biochemical tests Respective protein levels in mouse sera and culture media were evaluated using ELISA kits for OPG (R & D Systems), IFN-β (BD PharMingen), or insulin (Morinaga Institute of Biological Science).

    Techniques: Derivative Assay

    Blocking of autophagy inhibits wear particle induced osteoclastogenesis in vitro. (a) The expression of TRAP, RANKL, and OPG was detected by real‐time PCR in RAW 264.7 cells under the indicated treatments. (b) RAW 264.7 cells were transfected with GFP‐LC3 for 24 h and then treated with Ti in the presence or absence of 3‐MA for 48 h to observe the punctuate GFP‐LC3 distribution by fluorescence microscopy; the expression of TRAP was also detected by immunofluorescence staining. (c) The cell culture supernatants in different groups were collected to detect RANKL and OPG with ELISA. (d) The expression of TRAP in RAW 264.7 cells in different groups was detected by western blotting. (e) Ti or 3‐MA treatment induced autophagy was detected by electron microscopy of bone sections. Data represent mean ± SEM; n = 5 (* p

    Journal: Cell Biology International

    Article Title: Netrin‐1 regulates ERK1/2 signaling pathway and autophagy activation in wear particle‐induced osteoclastogenesis. Netrin‐1 regulates ERK1/2 signaling pathway and autophagy activation in wear particle‐induced osteoclastogenesis

    doi: 10.1002/cbin.11544

    Figure Lengend Snippet: Blocking of autophagy inhibits wear particle induced osteoclastogenesis in vitro. (a) The expression of TRAP, RANKL, and OPG was detected by real‐time PCR in RAW 264.7 cells under the indicated treatments. (b) RAW 264.7 cells were transfected with GFP‐LC3 for 24 h and then treated with Ti in the presence or absence of 3‐MA for 48 h to observe the punctuate GFP‐LC3 distribution by fluorescence microscopy; the expression of TRAP was also detected by immunofluorescence staining. (c) The cell culture supernatants in different groups were collected to detect RANKL and OPG with ELISA. (d) The expression of TRAP in RAW 264.7 cells in different groups was detected by western blotting. (e) Ti or 3‐MA treatment induced autophagy was detected by electron microscopy of bone sections. Data represent mean ± SEM; n = 5 (* p

    Article Snippet: Proteins were transferred to polyvinylidene difluoride (PVDF) membranes and then blocked with 5% bovine serum albumin in Tris‐buffered saline with Tween 20 (TBST) before immunodetection with antibodies to the following proteins: LC‐3 (1:1000; Abcam), TRAP (1:1000; Abcam), Beclin1 (1:1000; Abcam), Netrin‐1 (1:1000; Abcam), RANKL (1:1000; Abcam), OPG (1:1000; Abcam), ERK (1:1000; CST), p‐ERK (1:1000; CST), β‐actin (1:1000, CST), Unc5b (200 µg/ml, Abcam), DCC (200 µg/ml; Abcam) and recombinant Netrin‐1 (250 ng/ml; R & D).

    Techniques: Blocking Assay, In Vitro, Expressing, Real-time Polymerase Chain Reaction, Transfection, Fluorescence, Microscopy, Immunofluorescence, Staining, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot, Electron Microscopy

    Blocking of autophagy inhibits wear particle induced osteoclastogenesis in vivo. The air pouch mold was established, and Ti wear particles with or without 3‐MA were injected in the air pouch. (a) After 7 days, the implanted bones were harvested to detect the mRNA expression of TRAP and Netrin‐1 with real‐time PCR. (b) The harvested bones were also subjected to immunohistochemical staining to detect the expression of TRAP and Netrin‐1. (c) The mRNA expression of RANKL and OPG in pouch wall tissues was detected by real‐time PCR. (d) The protein expression of RANKL and OPG in pouch wall tissues was detected by western blotting. (e) The serum levels of RANKL and OPG were detected by ELISA. The mRNA expression of proinflammatory factors (IL‐1β, IL‐6, and TNF‐α) in pouch wall tissues was measured to assess the degree of inflammation. Data represent mean ± SEM; n = 5 (* p

    Journal: Cell Biology International

    Article Title: Netrin‐1 regulates ERK1/2 signaling pathway and autophagy activation in wear particle‐induced osteoclastogenesis. Netrin‐1 regulates ERK1/2 signaling pathway and autophagy activation in wear particle‐induced osteoclastogenesis

    doi: 10.1002/cbin.11544

    Figure Lengend Snippet: Blocking of autophagy inhibits wear particle induced osteoclastogenesis in vivo. The air pouch mold was established, and Ti wear particles with or without 3‐MA were injected in the air pouch. (a) After 7 days, the implanted bones were harvested to detect the mRNA expression of TRAP and Netrin‐1 with real‐time PCR. (b) The harvested bones were also subjected to immunohistochemical staining to detect the expression of TRAP and Netrin‐1. (c) The mRNA expression of RANKL and OPG in pouch wall tissues was detected by real‐time PCR. (d) The protein expression of RANKL and OPG in pouch wall tissues was detected by western blotting. (e) The serum levels of RANKL and OPG were detected by ELISA. The mRNA expression of proinflammatory factors (IL‐1β, IL‐6, and TNF‐α) in pouch wall tissues was measured to assess the degree of inflammation. Data represent mean ± SEM; n = 5 (* p

    Article Snippet: Proteins were transferred to polyvinylidene difluoride (PVDF) membranes and then blocked with 5% bovine serum albumin in Tris‐buffered saline with Tween 20 (TBST) before immunodetection with antibodies to the following proteins: LC‐3 (1:1000; Abcam), TRAP (1:1000; Abcam), Beclin1 (1:1000; Abcam), Netrin‐1 (1:1000; Abcam), RANKL (1:1000; Abcam), OPG (1:1000; Abcam), ERK (1:1000; CST), p‐ERK (1:1000; CST), β‐actin (1:1000, CST), Unc5b (200 µg/ml, Abcam), DCC (200 µg/ml; Abcam) and recombinant Netrin‐1 (250 ng/ml; R & D).

    Techniques: Blocking Assay, In Vivo, Injection, Expressing, Real-time Polymerase Chain Reaction, Immunohistochemistry, Staining, Western Blot, Enzyme-linked Immunosorbent Assay