Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Regression analysis in the newborn population. Results of the method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers. Graphs indicate Pearson correlation, slope and intercept for the main parameters. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: A method comparison study was undertaken to compare Sight OLO® with the Sysmex XN-9100™ System, the reference hematology analyzer presents at the Central Laboratory.

Techniques: Comparison

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Bias plot in the newborn population. Bland Altman of the results of a method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers for the main measurements in the newborn population. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: A method comparison study was undertaken to compare Sight OLO® with the Sysmex XN-9100™ System, the reference hematology analyzer presents at the Central Laboratory.

Techniques: Comparison

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Bias plot in the adult population. Bland Altman of the results of method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers for the main measurands in the adult population. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: A method comparison study was undertaken to compare Sight OLO® with the Sysmex XN-9100™ System, the reference hematology analyzer presents at the Central Laboratory.

Techniques: Comparison

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Instrumental analytical alarms (flags) concordance between Sight OLO® and Sysmex XN™ in the newborn population.

Article Snippet: A method comparison study was undertaken to compare Sight OLO® with the Sysmex XN-9100™ System, the reference hematology analyzer presents at the Central Laboratory.

Techniques:

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Instrumental analytical alarms (flags) concordance between Sight OLO® and Sysmex XN™ in the adult population.

Article Snippet: A method comparison study was undertaken to compare Sight OLO® with the Sysmex XN-9100™ System, the reference hematology analyzer presents at the Central Laboratory.

Techniques:

Journal:

Article Title: Requirement for Cellular Cyclin-Dependent Kinases in Herpes Simplex Virus Replication and Transcription

doi:

Figure Lengend Snippet: Concentrations of Rosco and Olo required to inhibit cell cycle progression in HEL and Vero cells. HEL (A, C, and E) and Vero (B and D) cells (3 × 105 to 6 × 105) were seeded in 35-mm dishes (330 to 600 cells/mm2) in the presence or absence of the indicated concentrations of Rosco (A and B), Olo (C and D), or Lova (E). Because of the short duration of some of Olo biological effects, cultures treated with Olo required a medium change at 6 hpi. After 24 h, cells were harvested by trypsinization and resuspended in Telford’s reagent and cellular DNA content was determined by FACS analysis. The percentages of cells in specific phases of the cell cycle are plotted against the drug concentration.

Article Snippet: Olo was purchased from Promega (Madison, Wis.); Rosco and iso-Olo were purchased from Calbiochem (San Diego, Calif.).

Techniques: Concentration Assay

Journal:

Article Title: Requirement for Cellular Cyclin-Dependent Kinases in Herpes Simplex Virus Replication and Transcription

doi:

Figure Lengend Snippet: Lova, staurosporine, iso-Olo, and PD98059 do not inhibit HSV-1 replication. HEL cells (2 × 105) in 12-well plates (500 cells/mm2) were infected with 2.5 PFU of HSV-1 KOS per cell. After adsorption for 1 h at 37°C, the inoculum was removed, the cells were washed twice with cold PBS, and control medium lacking drugs or containing the indicated concentration of the indicated drug was added. The drug concentrations were: 40 μM Rosco, 75 μM Olo or iso-Olo, 20 μM Lova, 5 ng of staurosporine (Stau)/ml, and 70 μM PD98059 (PD). These concentrations of drugs had no toxic effects on uninfected HEL cells for at least 24 h as determined by microscopic evaluation. Cultures treated with Olo required a medium change at 6 hpi. At the indicated times postinfection, cells were harvested, frozen, thawed, and sonicated and the virus was titrated by standard plaque assay. Viral titers are plotted against time postinfection (where time zero is the time of virus addition to cultured cells). Each time point indicates the average and range of two experiments. Results are presented as two graphs, A and B, for clarity; consequently, the no-treatment (Control) and Rosco-treated (Rosco) controls are shown in both panels.

Article Snippet: Olo was purchased from Promega (Madison, Wis.); Rosco and iso-Olo were purchased from Calbiochem (San Diego, Calif.).

Techniques: Infection, Adsorption, Concentration Assay, Sonication, Plaque Assay, Cell Culture

Journal:

Article Title: Requirement for Cellular Cyclin-Dependent Kinases in Herpes Simplex Virus Replication and Transcription

doi:

Figure Lengend Snippet: Concentrations of Rosco and Olo required to inhibit HSV-1 replication in HEL and Vero cells. HEL or Vero cells (2 × 105) in 12-well plates (500 cells/mm2) were infected with 2.5 PFU of HSV-1 strain KOS per cell. One hour later, the inoculum was removed, the cells were washed twice with cold PBS, and medium containing the indicated concentrations of Rosco (A), Olo (B), or PAA (C) was added. Cultures treated with Olo required a medium change at 6 hpi. After 24 h, cells were harvested and virus was titrated by standard plaque assay. Viral titers at 24 hpi are plotted against the drug concentration. Note that the scales on the y axis differ between panel B and panels A and C. Each time point indicates the average and range of two experiments.

Article Snippet: Olo was purchased from Promega (Madison, Wis.); Rosco and iso-Olo were purchased from Calbiochem (San Diego, Calif.).

Techniques: Infection, Plaque Assay, Concentration Assay

Journal:

Article Title: Requirement for Cellular Cyclin-Dependent Kinases in Herpes Simplex Virus Replication and Transcription

doi:

Figure Lengend Snippet: Absence of Rosco- or Olo-resistant variants of HSV after 11 passages in selective medium. HSV-1 strain KOS was passaged in medium containing either Olo, Rosco, or PAA as described in the text and in Table ​Table1,1, footnote a. After 11 passages in culture, 1,000 PFU of each passage was used to infect 105 Vero cells in duplicate. One set of infected cells was treated with inhibitory concentrations of the selective drugs (100 μg of PAA/ml, 100 μM Rosco, or 150 μM Olo), while the other set was left untreated (medium without drug). Virus was harvested and titrated at 24 hpi. The medium in cultures treated with Olo was changed at 6 hpi. The percentage of resistant virus after each passage was calculated by using the formula percent resistance = 100 × (PFU in the presence of selection drug/PFU in medium without drug) and is plotted against the passage number. (A) Percentage of virus resistant to Olo, Rosco, or PAA. The percentage of virus resistant to Rosco in the four different Rosco passage series (ROSCO 1, 1b, 9, and 9b) was so low as to be indistinguishable on this scale. Therefore, only the ROSCO 1 selection series was plotted. (B) Percentage of virus resistant to Rosco at different passages in the four Rosco selection series. Note the expanded scale of the y axis. The percentage of virus resistant to Olo or PAA was higher than 0.2% from the first passage on. Thus, the Olo and PAA selection series could not be plotted on this graph. ROSCO 1, selection of HSV-1 KOS in high Rosco concentrations (75, 50, 30, 30, and 50 μM from passages 7 to 11, respectively); ROSCO 1b, selection of HSV-1 KOS in low Rosco concentrations (40, 30, 30, and 50 μM from passages 7 to 10, respectively); ROSCO 9, selection in high Rosco concentrations (75, 50, 30, 30, and 50 μM from passages 7 to 11, respectively) of an HSV-1 stock previously passaged nine times in Olo; ROSCO 9b, selection in low Rosco concentrations (40, 30, 30, and 50 μM from passages 7 to 10, respectively) of an HSV-1 stock previously passaged nine times in Olo. See Table ​Table11 and the text for details.

Article Snippet: Olo was purchased from Promega (Madison, Wis.); Rosco and iso-Olo were purchased from Calbiochem (San Diego, Calif.).

Techniques: Infection, Selection

Journal:

Article Title: Requirement for Cellular Cyclin-Dependent Kinases in Herpes Simplex Virus Replication and Transcription

doi:

Figure Lengend Snippet: Viral DNA replication in the presence and absence of Olo or Rosco. HEL cells (9 × 105) in 60-mm dishes (320 cells/mm2) were infected with 2.5 PFU of HSV-1 per cell in the presence of 75 μM Olo (OLO) or 40 μM Rosco (ROSCO) or in the absence of a drug (CONTROL). DNA was extracted from infected cells at 1, 3, 6, 9, 12, and 18 hpi and from mock-infected cells (MI). Five micrograms of total DNA was blotted onto a nitrocellulose membrane, UV cross-linked, and hybridized to labeled HSV-specific riboprobes, and the membrane was exposed in a PhosphorImager (A). The signal hybridized to each slot was quantitated and corrected for loading as measured by GAPDH hybridization, and the amount of viral DNA in each sample at the indicated time after infection was plotted (B). The insert in panel B shows the amounts of viral DNA detected during the interval between 1 and 3 hpi on an expanded scale.

Article Snippet: Olo was purchased from Promega (Madison, Wis.); Rosco and iso-Olo were purchased from Calbiochem (San Diego, Calif.).

Techniques: Infection, Labeling, Hybridization

Journal:

Article Title: Requirement for Cellular Cyclin-Dependent Kinases in Herpes Simplex Virus Replication and Transcription

doi:

Figure Lengend Snippet: Viral RNA accumulation in the presence and absence of Olo or Rosco. HEL cells (7.5 × 105) in 60-mm dishes (275 cells/mm2) were infected with 2.5 PFU of HSV-1 per cell in the presence of 75 μM Olo (O) or 40 μM Rosco (R) or in the absence of a drug (C), and total (cellular and viral) RNA was extracted at 2, 5, 8, 12, or 15 hpi, as well as from mock-infected cells (MI). Levels of GAPDH-, ICP4-, TK-, and ICP8-specific RNAs were measured by standard RNase protection assays (ProtectDirect; Ambion) as recommended by the manufacturer, with minor modifications.

Article Snippet: Olo was purchased from Promega (Madison, Wis.); Rosco and iso-Olo were purchased from Calbiochem (San Diego, Calif.).

Techniques: Infection

Journal:

Article Title: Requirement for Cellular Cyclin-Dependent Kinases in Herpes Simplex Virus Replication and Transcription

doi:

Figure Lengend Snippet: Comparison of cdk-2 amino acids that interact with Rosco and Olo among cdks inhibited and not inhibited by these drugs. A comparison of the cdk-2 amino acids that interact with Rosco and Olo in all other recognized cdks, as well as in erk-1 and erk-2, was made by sequence analysis. The sequence of erk-2 (not shown) is identical to that of erk-1 in the relevant region. Alignment of the sequences was performed by using the Pileup program (GCG software package) with a gap weight of 12 and a gap extension weight of 4 (default values for both parameters). Analysis of the conserved residues shown by others to interact with ATP, Olo, or Rosco (11, 55) was performed manually. Only the relevant segments of the sequences are shown; the values in parentheses are the numbers of amino acids not shown. The numbers at the top correspond to the sequence of cdk-1 (cdc2). Boxed amino acids are those identical to the amino acids of cdk-2 that interact with ATP, as determined by Schulze-Gahmen and colleagues (52) and De Azevedo and colleagues (11). Note that nearly all of these amino acids are conserved. Amino acids of cdk-2 that interact with Rosco and Olo, as determined by Schulze-Gahmen and colleagues (52) and De Azevedo and colleagues (11), and their homologs in other cdks are enclosed by ovals; some of these residues also interact with ATP. Note that some amino acids are conserved in cdks known to be inhibited by Rosco and Olo but not in cdks known not to be inhibited by these drugs. The asterisks indicate the cdk predicted by this analysis to be inhibited by Rosco and Olo. The triangles indicate the cdks predicted by this analysis not to be inhibited by Rosco and Olo.

Article Snippet: Olo was purchased from Promega (Madison, Wis.); Rosco and iso-Olo were purchased from Calbiochem (San Diego, Calif.).

Techniques: Sequencing, Software

Journal: BMC Pharmacology & Toxicology

Article Title: Antihistamine effects and safety of fexofenadine: a systematic review and Meta-analysis of randomized controlled trials

doi: 10.1186/s40360-019-0363-1

Figure Lengend Snippet: Characteristics of included studies

Article Snippet: Kamei [ ], 2003 , RCT, crossover study , healthy , 11 , FEX 120 mg, n = 11 , d-CHL 4 mg, n = 11; OLO 10 mg, n = 11; PL n = 11 , 8 h , AE, sedative effects, CFF, CRT, CTT, LARS.

Techniques:

Journal: Journal of Ophthalmology

Article Title: Biodegradable PTLGA Terpolymers versus Collagen Implants Used as an Adjuvant in Trabeculectomy in Rabbit Eye

doi: 10.1155/2015/737198

Figure Lengend Snippet: Comparison of IOP measurements between the Ologen group and PTLGA terpolymer groups.

Article Snippet: OLO (Ologen; Aeon Astron Group B.V. Leiden, the Netherlands).

Techniques: Comparison

Journal: Journal of Ophthalmology

Article Title: Biodegradable PTLGA Terpolymers versus Collagen Implants Used as an Adjuvant in Trabeculectomy in Rabbit Eye

doi: 10.1155/2015/737198

Figure Lengend Snippet: Comparison of complications between the Ologen group and PTLGA terpolymer groups.

Article Snippet: OLO (Ologen; Aeon Astron Group B.V. Leiden, the Netherlands).

Techniques: Comparison

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Regression analysis in the newborn population. Results of the method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers. Graphs indicate Pearson correlation, slope and intercept for the main parameters. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques: Comparison

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Bias plot in the newborn population. Bland Altman of the results of a method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers for the main measurements in the newborn population. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques: Comparison

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Bias plot in the adult population. Bland Altman of the results of method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers for the main measurands in the adult population. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques: Comparison

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Instrumental analytical alarms (flags) concordance between Sight OLO® and Sysmex XN™ in the newborn population.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques:

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Instrumental analytical alarms (flags) concordance between Sight OLO® and Sysmex XN™ in the adult population.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques: