oligos Illumina Inc Search Results


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  • 99
    New England Biolabs nebnext multiplex oligos
    Nebnext Multiplex Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext multiplex oligos/product/New England Biolabs
    Average 99 stars, based on 3806 article reviews
    Price from $9.99 to $1999.99
    nebnext multiplex oligos - by Bioz Stars, 2020-04
    99/100 stars
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    93
    Illumina Inc oligonucleotides “oligos
    Oligonucleotides “Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligonucleotides “oligos/product/Illumina Inc
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    oligonucleotides “oligos - by Bioz Stars, 2020-04
    93/100 stars
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    96
    Illumina Inc oligos
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligos/product/Illumina Inc
    Average 96 stars, based on 364 article reviews
    Price from $9.99 to $1999.99
    oligos - by Bioz Stars, 2020-04
    96/100 stars
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    99
    Illumina Inc multiplex oligos
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Multiplex Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex oligos/product/Illumina Inc
    Average 99 stars, based on 332 article reviews
    Price from $9.99 to $1999.99
    multiplex oligos - by Bioz Stars, 2020-04
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    80
    Illumina Inc adapter oligonucleotides
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Adapter Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adapter oligonucleotides/product/Illumina Inc
    Average 80 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    adapter oligonucleotides - by Bioz Stars, 2020-04
    80/100 stars
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    86
    Illumina Inc surface linked oligonucleotides
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Surface Linked Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/surface linked oligonucleotides/product/Illumina Inc
    Average 86 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    surface linked oligonucleotides - by Bioz Stars, 2020-04
    86/100 stars
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    86
    Illumina Inc mouse oligonucleotides
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Mouse Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse oligonucleotides/product/Illumina Inc
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mouse oligonucleotides - by Bioz Stars, 2020-04
    86/100 stars
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    86
    Illumina Inc primer oligonucleotides
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Primer Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer oligonucleotides/product/Illumina Inc
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    primer oligonucleotides - by Bioz Stars, 2020-04
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    92
    Illumina Inc 6 mer oligonucleotides
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    6 Mer Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 mer oligonucleotides/product/Illumina Inc
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    6 mer oligonucleotides - by Bioz Stars, 2020-04
    92/100 stars
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    85
    Illumina Inc human oligonucleotides
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Human Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human oligonucleotides/product/Illumina Inc
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    human oligonucleotides - by Bioz Stars, 2020-04
    85/100 stars
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    94
    Illumina Inc paired end adaptor oligonucleotides
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Paired End Adaptor Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paired end adaptor oligonucleotides/product/Illumina Inc
    Average 94 stars, based on 174 article reviews
    Price from $9.99 to $1999.99
    paired end adaptor oligonucleotides - by Bioz Stars, 2020-04
    94/100 stars
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    94
    Illumina Inc sequencing adapter oligos
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Sequencing Adapter Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing adapter oligos/product/Illumina Inc
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    sequencing adapter oligos - by Bioz Stars, 2020-04
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    91
    Illumina Inc paired end adapter oligonucleotides
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Paired End Adapter Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paired end adapter oligonucleotides/product/Illumina Inc
    Average 91 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    paired end adapter oligonucleotides - by Bioz Stars, 2020-04
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    94
    Illumina Inc nextera index oligos
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Nextera Index Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera index oligos/product/Illumina Inc
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nextera index oligos - by Bioz Stars, 2020-04
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    85
    Illumina Inc illumina multiplexing oligos
    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative <t>PCR</t> for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture <t>oligos</t> and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.
    Illumina Multiplexing Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina multiplexing oligos/product/Illumina Inc
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    illumina multiplexing oligos - by Bioz Stars, 2020-04
    85/100 stars
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    96
    Illumina Inc brca2 oligonucleotides
    Relative expression of BRCA1 and <t>BRCA2</t> mRNA isoforms in rare variant samples compared to controls. (A) Natural expression ranges of mRNA splice isoforms calculated from lymphoblastoid cell lines (LCLs) not containing any known spliceogenic variants in BRCA1 (A) and BRCA2 (C) . Colored symbols overlaid indicate the relative mRNA isoform expression in LCLs containing known BRCA1 (B) or BRCA2 (D) splice disrupting variants. Only mRNA splice isoforms that were detected by more than 10 reads in at least two controls were included. Mean and upper and lower limits shown for each isoform [SE (95%)].
    Brca2 Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brca2 oligonucleotides/product/Illumina Inc
    Average 96 stars, based on 23 article reviews
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    brca2 oligonucleotides - by Bioz Stars, 2020-04
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    94
    Illumina Inc gene specific oligonucleotides
    Relative expression of BRCA1 and <t>BRCA2</t> mRNA isoforms in rare variant samples compared to controls. (A) Natural expression ranges of mRNA splice isoforms calculated from lymphoblastoid cell lines (LCLs) not containing any known spliceogenic variants in BRCA1 (A) and BRCA2 (C) . Colored symbols overlaid indicate the relative mRNA isoform expression in LCLs containing known BRCA1 (B) or BRCA2 (D) splice disrupting variants. Only mRNA splice isoforms that were detected by more than 10 reads in at least two controls were included. Mean and upper and lower limits shown for each isoform [SE (95%)].
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    Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from <t>microarray-synthesized</t> oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.
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    Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from <t>microarray-synthesized</t> oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.
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    Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from <t>microarray-synthesized</t> oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.
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    Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from <t>microarray-synthesized</t> oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.
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    Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from <t>microarray-synthesized</t> oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.
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    Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from <t>microarray-synthesized</t> oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.
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    Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from <t>microarray-synthesized</t> oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.
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    Differential hybridization mapping within positive PCR fragment sequences. ( A ) Hybridization to multiple <t>60-nt</t> oligonucleotides positioned opposite an intron sequence annotated on the antisense strand. ( B ) Hybridization to oligonucleotides representing a predicted exon within an annotated intron on the sense strand. ( C ) Control spots showing differential hybridization to a known exon (1) located on the strand opposite an annotated intron and (2) whose expression was previously verified. NCBI/UCSC sequence coordinates are offset by 13 Mb to approximate the size of the unsequenced p arm.
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    Differential hybridization mapping within positive PCR fragment sequences. ( A ) Hybridization to multiple <t>60-nt</t> oligonucleotides positioned opposite an intron sequence annotated on the antisense strand. ( B ) Hybridization to oligonucleotides representing a predicted exon within an annotated intron on the sense strand. ( C ) Control spots showing differential hybridization to a known exon (1) located on the strand opposite an annotated intron and (2) whose expression was previously verified. NCBI/UCSC sequence coordinates are offset by 13 Mb to approximate the size of the unsequenced p arm.
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    Differential hybridization mapping within positive PCR fragment sequences. ( A ) Hybridization to multiple <t>60-nt</t> oligonucleotides positioned opposite an intron sequence annotated on the antisense strand. ( B ) Hybridization to oligonucleotides representing a predicted exon within an annotated intron on the sense strand. ( C ) Control spots showing differential hybridization to a known exon (1) located on the strand opposite an annotated intron and (2) whose expression was previously verified. NCBI/UCSC sequence coordinates are offset by 13 Mb to approximate the size of the unsequenced p arm.
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    Image Search Results


    Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative PCR for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture oligos and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.

    Journal: Oncotarget

    Article Title: Hierarchical regulation of the genome: global changes in nucleosome organization potentiate genome response

    doi: 10.18632/oncotarget.6841

    Figure Lengend Snippet: Mapping nucleosome distributions following KSHV reactivation using mTSS-seq technique and mTSS-seq validation A. Experimental design for mapping nucleosome distributions following KSHV reactivation using our newly developed mTSS-seq technique. B. Validation of mTSS-seq using quantitative PCR for both on-target regions of the genome (within the sequence-capture region, two-kb region centered on a TSS) and off-target regions of the genome (not within the sequence-capture region, outside the two-region centered on a TSS). Quantitative PCR was performed for on- and off-target regions of the genome for both RHOC and ITGA4. The y-axis shows C t values. On average, C t values between the on- and off-targets of the sequence-captured libraries differ by 10.5 cycles. C. The periodic occurrence of AA/TT/AT/TA dinucleotides was calculated for all nucleosomal-sized fragments (147-148 bp) at the 0 hour time point. The x-axis represents the distance from the dyad axis. The y-axis is the frequency of AA/TT/AT/TA dinucleotides. An A/T-containing dinucleotide periodicity is seen every 10 bp at the 0 hour time point. D. Alignment of the midpoint fragments (purple) from mTSS-seq to the human genome shown in the UCSC Genome Browser ( http://genome.ucsc.edu ). Zooming in 5000X on human chromosome 2 to a six-kb window with two-kb of midpoint fragments at 0 hour time point (purple lines), along with the sequence-capture oligos and previously-published human-nucleosome distribution map for cell line GM18508 (red lines, (Gaffney et al., 2012)) at the TSSs of PUS10 and PEX13. The x-axis is genomic location. The y-axis is scaled reads per million.

    Article Snippet: The universal and indexed sequences were added by PCR using 23 ul of adaptor-ligated DNA fragments, NEBNext High Fidelity 2X PCR Master Mix, index primers provided in NEBNext Multiplex (NEB #E7335, #E7500) Oligos for Illumina, and Universal PCR Primers provided in NEBNext Multiplex (NEB #E7335, #E7500) Oligos for Illumina.

    Techniques: Real-time Polymerase Chain Reaction, Sequencing

    Diagram of tGBS. Digestion . Genomic DNA is digested with two REs: NspI leaves a 3′overhang and BfuCI leaves a 5′ overhang. Ligation . Two distinct oligos are ligated to the complementary 3′ and 5′ overhangs. The oligo matching the 3′ overhang contains a sample-specific internal barcode sequence for sample identification. The oligo matching the 5′ overhang is universal and present in every reaction for later amplification. Selective PCR . Target sites are selected using a selective primer with variable selective bases (‘CA’) that match selected sequences in the digested genome fragments and a non-selective primer. When properly amplified, the selected sequence is complementary to the selective bases. Final PCR . Primers matching the amplification primer and the selective primer which contain the full Proton adaptor sequence are used for amplification of the final library. Final on-target sequence . The final sequence contains the 5′ Proton adaptor sequence, an internal barcode, the NspI RE site, the target molecule, selective bases, the BfuCI RE site and the 3′ Proton adaptor sequence. It is possible to adapt the tGBS protocol for sequencing on an Illumina instrument by redesigning the ligation oligos and PCR primers.

    Journal: Nucleic Acids Research

    Article Title: tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci

    doi: 10.1093/nar/gkx853

    Figure Lengend Snippet: Diagram of tGBS. Digestion . Genomic DNA is digested with two REs: NspI leaves a 3′overhang and BfuCI leaves a 5′ overhang. Ligation . Two distinct oligos are ligated to the complementary 3′ and 5′ overhangs. The oligo matching the 3′ overhang contains a sample-specific internal barcode sequence for sample identification. The oligo matching the 5′ overhang is universal and present in every reaction for later amplification. Selective PCR . Target sites are selected using a selective primer with variable selective bases (‘CA’) that match selected sequences in the digested genome fragments and a non-selective primer. When properly amplified, the selected sequence is complementary to the selective bases. Final PCR . Primers matching the amplification primer and the selective primer which contain the full Proton adaptor sequence are used for amplification of the final library. Final on-target sequence . The final sequence contains the 5′ Proton adaptor sequence, an internal barcode, the NspI RE site, the target molecule, selective bases, the BfuCI RE site and the 3′ Proton adaptor sequence. It is possible to adapt the tGBS protocol for sequencing on an Illumina instrument by redesigning the ligation oligos and PCR primers.

    Article Snippet: We have tested Illumina oligos and barcodes ( ) and obtained similar levels of accuracy as reported for the Ion Proton platform (data not shown).

    Techniques: Ligation, Sequencing, Amplification, Polymerase Chain Reaction

    Purification of RNA oligomers and DNA amplimers after the CLIP procedure. ( a ) Autoradiograph of the 32 P-labeled RNA oligonucleotides with various sizes isolated as described in the text and subsequently ligated to the RA3 and RA5 adapters using the Illumina’s

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: High-Throughput Sequencing of RNA Isolated by Cross- Linking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs

    doi: 10.1007/978-1-62703-971-0_17

    Figure Lengend Snippet: Purification of RNA oligomers and DNA amplimers after the CLIP procedure. ( a ) Autoradiograph of the 32 P-labeled RNA oligonucleotides with various sizes isolated as described in the text and subsequently ligated to the RA3 and RA5 adapters using the Illumina’s

    Article Snippet: Illumina’s oligonucleotides are modified and purified in a proprietary manner ( see Note ): RNA 5′ adapter (RA5): 5′-GUUCAGAGUUCUACAGUCCGACGAUC-3′.

    Techniques: Purification, Cross-linking Immunoprecipitation, Autoradiography, Labeling, Isolation

    Relative expression of BRCA1 and BRCA2 mRNA isoforms in rare variant samples compared to controls. (A) Natural expression ranges of mRNA splice isoforms calculated from lymphoblastoid cell lines (LCLs) not containing any known spliceogenic variants in BRCA1 (A) and BRCA2 (C) . Colored symbols overlaid indicate the relative mRNA isoform expression in LCLs containing known BRCA1 (B) or BRCA2 (D) splice disrupting variants. Only mRNA splice isoforms that were detected by more than 10 reads in at least two controls were included. Mean and upper and lower limits shown for each isoform [SE (95%)].

    Journal: Frontiers in Oncology

    Article Title: Investigation of Experimental Factors That Underlie BRCA1/2 mRNA Isoform Expression Variation: Recommendations for Utilizing Targeted RNA Sequencing to Evaluate Potential Spliceogenic Variants

    doi: 10.3389/fonc.2018.00140

    Figure Lengend Snippet: Relative expression of BRCA1 and BRCA2 mRNA isoforms in rare variant samples compared to controls. (A) Natural expression ranges of mRNA splice isoforms calculated from lymphoblastoid cell lines (LCLs) not containing any known spliceogenic variants in BRCA1 (A) and BRCA2 (C) . Colored symbols overlaid indicate the relative mRNA isoform expression in LCLs containing known BRCA1 (B) or BRCA2 (D) splice disrupting variants. Only mRNA splice isoforms that were detected by more than 10 reads in at least two controls were included. Mean and upper and lower limits shown for each isoform [SE (95%)].

    Article Snippet: The targeted sequencing assay was custom designed in Illumina’s design studio using 34 BRCA1 and 28 BRCA2 oligonucleotides chosen from a database of validated predesigned probes (Tables S2 and S3 and Figures S2 and S3 in Supplementary Material).

    Techniques: Expressing, Variant Assay

    BRCA2 mRNA isoforms detected at six time points in an lymphoblastoid cell line (sample #7, Table S1 in Supplementary Material) treated with an nonsense-mediated decay inhibitor. A freeze–thaw process was undertaken after time points two and four. Three technical replicates are listed under each time point.

    Journal: Frontiers in Oncology

    Article Title: Investigation of Experimental Factors That Underlie BRCA1/2 mRNA Isoform Expression Variation: Recommendations for Utilizing Targeted RNA Sequencing to Evaluate Potential Spliceogenic Variants

    doi: 10.3389/fonc.2018.00140

    Figure Lengend Snippet: BRCA2 mRNA isoforms detected at six time points in an lymphoblastoid cell line (sample #7, Table S1 in Supplementary Material) treated with an nonsense-mediated decay inhibitor. A freeze–thaw process was undertaken after time points two and four. Three technical replicates are listed under each time point.

    Article Snippet: The targeted sequencing assay was custom designed in Illumina’s design studio using 34 BRCA1 and 28 BRCA2 oligonucleotides chosen from a database of validated predesigned probes (Tables S2 and S3 and Figures S2 and S3 in Supplementary Material).

    Techniques:

    Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from microarray-synthesized oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.

    Journal: Nucleic Acids Research

    Article Title: Highly selective retrieval of accurate DNA utilizing a pool of in situ-replicated DNA from multiple next-generation sequencing platforms

    doi: 10.1093/nar/gky016

    Figure Lengend Snippet: Schematic of in situ replication of DNA molecules from next-generation sequencing (NGS) platforms and subsequent PCR-based retrieval of target sequences. ( A ) Process flow chart for PCR-based methods for the retrieval of error-free DNA targets from an NGS-replica pool. ( B ) Preparation strategy of 454 GS Junior sequencing-based retrieval. Combinatorial barcode-tagged (CBT) pools were processed from microarray-synthesized oligonucleotides and subsequently ligated to the sheared genomic DNA as flanking sequences. The library was replicated in a sealed NGS plate. ( C ) Preparation strategy of a pre-NGS pool (MiSeq and Ion Proton). The barcoded library (cgc50 pool) was directly synthesized on a microarray. ( D ) Schematic of library replication in a MiSeq flow cell. ( E ) Schematic of library replication using melt-off DNA in the Ion Proton system. This process could be automatically performed using an Ion OneTouch™ ES system.

    Article Snippet: To use the CBT-based labeling method on the Illumina sequencer, another microarray oligonucleotides, consisting of cgc50 pool and CBT library, were redesigned and synthesized to be compatible with the Illumina platform ( ).

    Techniques: In Situ, Next-Generation Sequencing, Polymerase Chain Reaction, Flow Cytometry, Sequencing, Microarray, Synthesized

    Differential hybridization mapping within positive PCR fragment sequences. ( A ) Hybridization to multiple 60-nt oligonucleotides positioned opposite an intron sequence annotated on the antisense strand. ( B ) Hybridization to oligonucleotides representing a predicted exon within an annotated intron on the sense strand. ( C ) Control spots showing differential hybridization to a known exon (1) located on the strand opposite an annotated intron and (2) whose expression was previously verified. NCBI/UCSC sequence coordinates are offset by 13 Mb to approximate the size of the unsequenced p arm.

    Journal: Genes & Development

    Article Title: The transcriptional activity of human Chromosome 22

    doi: 10.1101/gad.1055203

    Figure Lengend Snippet: Differential hybridization mapping within positive PCR fragment sequences. ( A ) Hybridization to multiple 60-nt oligonucleotides positioned opposite an intron sequence annotated on the antisense strand. ( B ) Hybridization to oligonucleotides representing a predicted exon within an annotated intron on the sense strand. ( C ) Control spots showing differential hybridization to a known exon (1) located on the strand opposite an annotated intron and (2) whose expression was previously verified. NCBI/UCSC sequence coordinates are offset by 13 Mb to approximate the size of the unsequenced p arm.

    Article Snippet: The 60-nt oligonucleotides were purchased from Illumina.

    Techniques: Hybridization, Polymerase Chain Reaction, Sequencing, Expressing